Restriction map for
pFN21AE6306
(Circular) (Six-base) MAPSORT of: pFN21AE6306.seq Check: 3572 from: 1 to: 5671
pFN21AE6306 5671 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2952 3014 1937
Size: 1015 62 4594
Fragments arranged by size:
4594 1015 62
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3734 5118 5386 1632
Size: 2102 1384 268 1917
Fragments arranged by size:
2102 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 2499 4570 5547 1203
Size: 1296 2071 977 1327
Fragments arranged by size:
2071 1327 1296 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 2192 2474 2486 1166
Size: 1026 282 12 4351
Fragments arranged by size:
4351 1026 282 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2889 2921 2889
Size: 32 5639
AlwNI CAG_nnn'CTG
Cuts at: 2206 2717 3414 4986 2206
Size: 511 697 1572 2891
Fragments arranged by size:
2891 1572 697 511
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2980 3206 3358 5467 1493
Size: 1487 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 1870
Size: 73 1053 4545
Fragments arranged by size:
4545 1053 73
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2186 2539 2839 2992 4447
Size: 325 217 627 399 353 300 153 1455
Cuts at: 4447 618
Size: 1842
Fragments arranged by size:
1842 1455 627 399 353 325 300 217
153
BanII G_rGCy'C
Cuts at: 1391 2604 2990 1391
Size: 1213 386 4072
Fragments arranged by size:
4072 1213 386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 2304 1548
Size: 756 4915
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2555 4282
Size: 107 122 71 631 605 337 546 1727
Cuts at: 4282 136
Size: 1525
Fragments arranged by size:
1727 1525 631 605 546 337 122 107
71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 2813 3088 2813
Size: 275 5396
Bme1580I G_kGCm'C
Cuts at: 1790 2181 2544 3732 4888 1790
Size: 391 363 1188 1156 2573
Fragments arranged by size:
2573 1188 1156 391 363
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2146 2259 3402
Size: 416 53 83 399 24 729 113 1143
Cuts at: 3402 4360 442
Size: 958 1753
Fragments arranged by size:
1753 1143 958 729 416 399 113 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 4331 1239
Size: 1070 2022 2579
Fragments arranged by size:
2579 2022 1070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 2960 3796 4661 4959 2774
Size: 186 836 865 298 3486
Fragments arranged by size:
3486 865 836 298 186
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2972 4334 882
Size: 617 1473 1362 2219
Fragments arranged by size:
2219 1473 1362 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2889 2919 1178
Size: 30 165 30 1486 30 3930
Fragments arranged by size:
3930 1486 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5671
BseYI C'CCAG_C
Cuts at: 2535 4874 5370 2535
Size: 2339 496 2836
Fragments arranged by size:
2836 2339 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2774 1163
Size: 573 1038 4060
Fragments arranged by size:
4060 1038 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2604 3732 3817 4888 2604
Size: 1128 85 1071 3387
Fragments arranged by size:
3387 1128 1071 85
BsmI GAATG_Cn'
Cuts at: 2416 3182 3275 2416
Size: 766 93 4812
Fragments arranged by size:
4812 766 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2544 2604 2990 3732 3817 4888
Size: 399 391 363 60 386 742 85 1071
Cuts at: 4888 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 391 386 363 85
60
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2794 3026 844
Size: 1950 232 3489
Fragments arranged by size:
3489 1950 232
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 2817 3558 1394
Size: 429 302 692 741 3507
Fragments arranged by size:
3507 741 692 429 302
BsrDI GCAATG_nn'
Cuts at: 65 1248 2798 4166 4340 65
Size: 1183 1550 1368 174 1396
Fragments arranged by size:
1550 1396 1368 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2344 4315 4502 1700
Size: 110 534 1971 187 2869
Fragments arranged by size:
2869 1971 534 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2770 2809 794
Size: 1976 39 3656
Fragments arranged by size:
3656 1976 39
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 2294 2476 2488 2149
Size: 145 182 12 5332
Fragments arranged by size:
5332 182 145 12
BstYI r'GATC_y
Cuts at: 1972 3001 3368 3753 3770 5222 5308 5320 5666
Size: 1029 367 385 17 1452 86 12 346
Cuts at: 5666 1972
Size: 1977
Fragments arranged by size:
1977 1452 1029 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2481 513
Size: 552 939 477 3703
Fragments arranged by size:
3703 939 552 477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2369 2469 3187 3981 4001 2369
Size: 100 718 794 20 4039
Fragments arranged by size:
4039 794 718 100 20
ClaI AT'CG_AT
Cuts at: 1554 2034 1554
Size: 480 5191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2478 2667 3118
Size: 54 1425 50 275 18 648 189 451
Cuts at: 3118 4007 5636 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 648 451 275 189 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 3601 1353
Size: 800 1448 3423
Fragments arranged by size:
3423 1448 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2602 2602
Size: 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 3734 4331 5118 5386 1239
Size: 393 677 1425 597 787 268 1524
Fragments arranged by size:
1524 1425 787 677 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2785 2785
Size: 5671
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 2519 4818 1678
Size: 841 2299 2531
Fragments arranged by size:
2531 2299 841
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4312 4344 4386 4418 1373
Size: 32 135 32 2740 32 42 32 2626
Fragments arranged by size:
2740 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2395 2505 3015 3261 669
Size: 988 281 457 110 510 246 3079
Fragments arranged by size:
3079 988 510 457 281 246 110
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4785 4969 1305
Size: 3480 184 2007
Fragments arranged by size:
3480 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2480 10
Size: 54 1425 343 648 3201
Fragments arranged by size:
3201 1425 648 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2474 2486 2498 2834 2869 3629
Size: 648 1026 282 12 12 336 35 760
Cuts at: 3629 3988 4147 518
Size: 359 159 2042
Fragments arranged by size:
2042 1026 760 648 359 336 282 159
35 12 12
MspA1I CmG'CkG
Cuts at: 1606 2203 2535 2552 3087 3764 4912 5157 1606
Size: 597 332 17 535 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 597 535 332 245 17
NaeI GCC'GGC
Cuts at: 2346 2346
Size: 5671
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4180
Fragments arranged by size:
4180 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NgoMIV G'CCGG_C
Cuts at: 2344 2344
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2281 2503 3029 4574 1132
Size: 97 1052 222 526 1545 2229
Fragments arranged by size:
2229 1545 1052 526 222 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 2499 4570 2499
Size: 2071 3600
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PfoI T'CCnGG_A
Cuts at: 2654 2654
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2889 2921 3740 3772 5253 5285 2889
Size: 32 819 32 1481 32 3275
Fragments arranged by size:
3275 1481 819 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 2785
Size: 5671
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 3023 830
Size: 2193 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2203 2203
Size: 5671
SacI G_AGCT'C
Cuts at: 2604 2604
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SapI GCTCTTCn'nnn_
Cuts at: 2153 2153
Size: 5671
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2332 3019 4154 4835 5026 826
Size: 219 1287 687 1135 681 191 1471
Fragments arranged by size:
1471 1287 1135 687 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2753 2939 3775 4676 4938 820
Size: 197 926 810 186 836 901 262 1553
Fragments arranged by size:
1553 926 901 836 810 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2004 2142 2523 3110 513
Size: 552 939 138 381 587 3074
Fragments arranged by size:
3074 939 587 552 381 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2356 2527 2696 3698 3715 3868
Size: 1255 624 152 171 169 1002 17 153
Cuts at: 3868 4053 4498 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1002 624 445 185 171 169
153 152 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2604 2636 5631 5663 2604
Size: 32 2995 32 2612
Fragments arranged by size:
2995 2612 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2246 2149
Size: 97 5574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 2081 3800 2081
Size: 1719 3952
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI