Restriction map for
pFN21AE6305
(Circular) (Six-base) MAPSORT of: pFN21AE6305.seq Check: 1164 from: 1 to: 5935
pFN21AE6305 5935 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 3278 1937
Size: 1161 180 4594
Fragments arranged by size:
4594 1161 180
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2166 2985 3998 5382 5650 1632
Size: 534 819 1013 1384 268 1917
Fragments arranged by size:
1917 1384 1013 819 534 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 2551 4834 5811 1203
Size: 1348 2283 977 1327
Fragments arranged by size:
2283 1348 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 2966 1166
Size: 1800 4135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AlwNI CAG_nnn'CTG
Cuts at: 2056 2246 2288 2590 3008 3678 5250 2056
Size: 190 42 302 418 670 1572 2741
Fragments arranged by size:
2741 1572 670 418 302 190 42
ApaLI G'TGCA_C
Cuts at: 3992 5148 3992
Size: 1156 4779
ApoI r'AATT_y
Cuts at: 1493 2363 2390 2426 3042 3244 3470 3622 5731
Size: 870 27 36 616 202 226 152 2109
Cuts at: 5731 1493
Size: 1697
Fragments arranged by size:
2109 1697 870 616 226 202 152 36
27
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2652 3260 1870
Size: 73 709 608 4545
Fragments arranged by size:
4545 709 608 73
AvrII C'CTAG_G
Cuts at: 2072 2072
Size: 5935
BamHI G'GATC_C
Cuts at: 2904 3265 3632 2904
Size: 361 367 5207
Fragments arranged by size:
5207 367 361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2109 3256 4711 618
Size: 325 217 627 322 1147 1455 1842
Fragments arranged by size:
1842 1455 1147 627 325 322 217
BanII G_rGCy'C
Cuts at: 1391 3254 1391
Size: 1863 4072
BbeI G_GCGC'C
Cuts at: 2113 2113
Size: 5935
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4964
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4126 4160 1903
Size: 34 50 34 2105 34 3678
Fragments arranged by size:
3678 2105 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 2977 1548
Size: 1429 4506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2481 4546 136
Size: 107 122 71 631 605 809 2065 1525
Fragments arranged by size:
2065 1525 809 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 2199 2531 3352 2199
Size: 332 821 4782
Fragments arranged by size:
4782 821 332
Bme1580I G_kGCm'C
Cuts at: 1790 3996 5152 1790
Size: 2206 1156 2573
Fragments arranged by size:
2573 2206 1156
BmgBI CAC'GTC
Cuts at: 1318 2552 1318
Size: 1234 4701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3666 4624 442
Size: 416 53 83 399 24 2249 958 1753
Fragments arranged by size:
2249 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2268 4595 1239
Size: 1029 2327 2579
Fragments arranged by size:
2579 2327 1029
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4060 4925 5223 4060
Size: 865 298 4772
Fragments arranged by size:
4772 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4598 882
Size: 617 3099 2219
Fragments arranged by size:
3099 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3726 493
Size: 711 2522 2702
Fragments arranged by size:
2702 2522 711
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2110 3742 4124 4759
Size: 53 83 186 936 577 1632 382 635
Cuts at: 4759 275
Size: 1451
Fragments arranged by size:
1632 1451 936 635 577 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2982 4368 4766 5040 5187 5598 2982
Size: 1386 398 274 147 411 3319
Fragments arranged by size:
3319 1386 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2724 2754 1178
Size: 30 165 30 1321 30 4359
Fragments arranged by size:
4359 1321 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3138 1186
Size: 1952 3983
BseYI C'CCAG_C
Cuts at: 2861 5138 5634 2861
Size: 2277 496 3162
Fragments arranged by size:
3162 2277 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2990 1163
Size: 573 1254 4108
Fragments arranged by size:
4108 1254 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2092 3024 3199 3996 4081 5152 2092
Size: 932 175 797 85 1071 2875
Fragments arranged by size:
2875 1071 932 797 175 85
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
BsmBI CGTCTCn'nnnn_
Cuts at: 2779 2779
Size: 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 3024 3199 3254 3996 4081 5152
Size: 399 302 932 175 55 742 85 1071
Cuts at: 5152 1391
Size: 2174
Fragments arranged by size:
2174 1071 932 742 399 302 175 85
55
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 2407 3824 5554 2407
Size: 1417 1730 2788
Fragments arranged by size:
2788 1730 1417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 3290 844
Size: 1954 492 3489
Fragments arranged by size:
3489 1954 492
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 2203 3822 1394
Size: 429 239 141 1619 3507
Fragments arranged by size:
3507 1619 429 239 141
BsrDI GCAATG_nn'
Cuts at: 65 1248 4430 4604 65
Size: 1183 3182 174 1396
Fragments arranged by size:
3182 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4579 4766 1700
Size: 110 2769 187 2869
Fragments arranged by size:
2869 2769 187 110
BsrGI T'GTAC_A
Cuts at: 96 2490 96
Size: 2394 3541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 3995 5007 3995
Size: 1012 4923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2014 2056 2755 794
Size: 1220 42 699 3974
Fragments arranged by size:
3974 1220 699 42
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstYI r'GATC_y
Cuts at: 1972 2904 3265 3632 4017 4034 5486 5572 5584
Size: 932 361 367 385 17 1452 86 12
Cuts at: 5584 5930 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 932 385 367 361 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3099 3099
Size: 5935
Bsu36I CC'TnA_GG
Cuts at: 2939 2939
Size: 5935
BtgI C'CryG_G
Cuts at: 513 1065 2536 2964 513
Size: 552 1471 428 3484
Fragments arranged by size:
3484 1471 552 428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5196
Fragments arranged by size:
5196 683 56
BtsI GCAGTG_nn'
Cuts at: 2239 2732 3006 3081 3451 4245 4265 2239
Size: 493 274 75 370 794 20 3909
Fragments arranged by size:
3909 794 493 370 274 75 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 2395 3239 2395
Size: 844 5091
DraIII CAC_nnn'GTG
Cuts at: 2096 2096
Size: 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2728 4942 809
Size: 507 1412 2214 1802
Fragments arranged by size:
2214 1802 1412 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3153 3382 4271
Size: 54 1425 50 275 18 1323 229 889
Cuts at: 4271 5900 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1323 889 275 229 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3217 3865 1353
Size: 1864 648 3423
Fragments arranged by size:
3423 1864 648
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2013 4522 4896 5042 387
Size: 1064 138 424 2509 374 146 1280
Fragments arranged by size:
2509 1280 1064 424 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2268 2985 3998 4595 5382 5650
Size: 393 534 102 717 1013 597 787 268
Cuts at: 5650 1239
Size: 1524
Fragments arranged by size:
1524 1013 787 717 597 534 393 268
102
EcoO109I rG'GnC_Cy
Cuts at: 2034 2914 2034
Size: 880 5055
EcoRI G'AATT_C
Cuts at: 2363 2426 3042 3244 3622 2363
Size: 63 616 202 378 4676
Fragments arranged by size:
4676 616 378 202 63
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 2084 2113 5082 1678
Size: 406 29 2969 2531
Fragments arranged by size:
2969 2531 406 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2038 2070 4576 4608 4650
Size: 32 135 32 466 32 2506 32 42
Cuts at: 4650 4682 1373
Size: 32 2626
Fragments arranged by size:
2626 2506 466 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2280 3279 3525 669
Size: 988 281 342 999 246 3079
Fragments arranged by size:
3079 999 988 342 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KasI G'GCGC_C
Cuts at: 2109 2109
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5049 5233 1305
Size: 3744 184 2007
Fragments arranged by size:
3744 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4113
Fragments arranged by size:
4113 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2966 3017 3893 4252 4411 518
Size: 648 1800 51 876 359 159 2042
Fragments arranged by size:
2042 1800 876 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 3351 4028 5176 5421 1606
Size: 1745 677 1148 245 2120
Fragments arranged by size:
2120 1745 1148 677 245
NarI GG'CG_CC
Cuts at: 2110 2110
Size: 5935
NcoI C'CATG_G
Cuts at: 513 1065 2964 513
Size: 552 1899 3484
Fragments arranged by size:
3484 1899 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 3293 4838 1132
Size: 97 2064 1545 2229
Fragments arranged by size:
2229 2064 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2718 1108
Size: 1610 4325
PfoI T'CCnGG_A
Cuts at: 2900 2907 2900
Size: 7 5928
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2914 2914
Size: 5935
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 2874 3505 2874
Size: 631 5304
PstI C_TGCA'G
Cuts at: 830 2234 3287 830
Size: 1404 1053 3478
Fragments arranged by size:
3478 1404 1053
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 3287 3287
Size: 5935
ScaI AGT'ACT
Cuts at: 2453 4183 5895 2453
Size: 1730 1712 2493
Fragments arranged by size:
2493 1730 1712
SfcI C'TryA_G
Cuts at: 826 1045 2230 2777 3283 4418 5099 5290 826
Size: 219 1185 547 506 1135 681 191 1471
Fragments arranged by size:
1471 1185 1135 681 547 506 219 191
SfoI GGC'GCC
Cuts at: 2111 2111
Size: 5935
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 3262 1872
Size: 1390 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4039 4940 5202 820
Size: 197 926 2096 901 262 1553
Fragments arranged by size:
2096 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 2484 1517
Size: 967 4968
StyI C'CwwG_G
Cuts at: 513 1065 2072 2964 3177 3374 513
Size: 552 1007 892 213 197 3074
Fragments arranged by size:
3074 1007 892 552 213 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3962 3979 4132 4317 4762 325
Size: 1255 2382 17 153 185 445 1498
Fragments arranged by size:
2382 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2451 2490 4181
Size: 274 80 33 51 1443 474 39 1691
Cuts at: 4181 5893 96
Size: 1712 138
Fragments arranged by size:
1712 1691 1443 474 274 138 80 51
39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2724 2756 5895 5927 2724
Size: 32 3139 32 2732
Fragments arranged by size:
3139 2732 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2284 1316
Size: 968 4967
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 3260 1870
Size: 1390 4545
XmnI GAAnn'nnTTC
Cuts at: 2998 4064 2998
Size: 1066 4869
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI