Restriction map for
pFN21AE6303
(Circular) (Six-base) MAPSORT of: pFN21AE6303.seq Check: 6014 from: 1 to: 5746
pFN21AE6303 5746 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 2058 2603 3067 2058
Size: 545 464 4737
Fragments arranged by size:
4737 545 464
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2371 2965 3809 5193 5461 1632
Size: 635 104 594 844 1384 268 1917
Fragments arranged by size:
1917 1384 844 635 594 268 104
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 3489 5061 3489
Size: 1572 4174
ApaI G_GGCC'C
Cuts at: 2014 2014
Size: 5746
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 2219 2228 3055 3281 3433 5542 1493
Size: 726 9 827 226 152 2109 1697
Fragments arranged by size:
2109 1697 827 726 226 152 9
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2117 3071 1870
Size: 73 70 104 954 4545
Fragments arranged by size:
4545 954 104 73 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2587 2620 2587
Size: 33 5713
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2058 2239 2603 3067 4522
Size: 325 217 627 271 181 364 464 1455
Cuts at: 4522 618
Size: 1842
Fragments arranged by size:
1842 1455 627 464 364 325 271 217
181
BanII G_rGCy'C
Cuts at: 1391 2014 3065 1391
Size: 623 1051 4072
Fragments arranged by size:
4072 1051 623
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2965 928
Size: 971 1066 3709
Fragments arranged by size:
3709 1066 971
BbvCI CC'TCA_GC
Cuts at: 2289 2289
Size: 5746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2098 2132 2895 2929 3937 3971 1903
Size: 34 161 34 763 34 1008 34 3678
Fragments arranged by size:
3678 1008 763 161 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BfrBI ATG'CAT
Cuts at: 2461 2461
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2293 4357
Size: 107 122 71 631 605 337 284 2064
Cuts at: 4357 136
Size: 1525
Fragments arranged by size:
2064 1525 631 605 337 284 122 107
71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 2529 3163 2529
Size: 634 5112
Bme1580I G_kGCm'C
Cuts at: 1790 2014 3807 4963 1790
Size: 224 1793 1156 2573
Fragments arranged by size:
2573 1793 1156 224
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4406 1239
Size: 3167 2579
Bpu10I CC'TnA_GC
Cuts at: 2289 2289
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4409 882
Size: 617 2910 2219
Fragments arranged by size:
2910 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 3935 4570 275
Size: 53 83 186 936 2020 382 635 1451
Fragments arranged by size:
2020 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5746
BseYI C'CCAG_C
Cuts at: 4949 5445 4949
Size: 496 5250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2329 1163
Size: 573 593 4580
Fragments arranged by size:
4580 593 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3807 3892 4963 3807
Size: 85 1071 4590
Fragments arranged by size:
4590 1071 85
BsmI GAATG_Cn'
Cuts at: 2872 3257 3350 2872
Size: 385 93 5268
Fragments arranged by size:
5268 385 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 3065 3807 3892 4963 1391
Size: 399 224 1051 742 85 1071 2174
Fragments arranged by size:
2174 1071 1051 742 399 224 85
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 3101 844
Size: 1226 1031 3489
Fragments arranged by size:
3489 1226 1031
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2607 4241 4415 65
Size: 1183 1359 1634 174 1396
Fragments arranged by size:
1634 1396 1359 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 2915 4550 2915
Size: 1635 4111
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstXI CCAn_nnnn'nTGG
Cuts at: 2516 2614 2759 2887 2516
Size: 98 145 128 5375
Fragments arranged by size:
5375 145 128 98
BstYI r'GATC_y
Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741
Size: 1104 367 385 17 1452 86 12 346
Cuts at: 5741 1972
Size: 1977
Fragments arranged by size:
1977 1452 1104 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2700 2758 2880 3016 513
Size: 552 939 696 58 122 136 3243
Fragments arranged by size:
3243 939 696 552 136 122 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2937 487
Size: 683 56 1711 3296
Fragments arranged by size:
3296 1711 683 56
BtsI GCAGTG_nn'
Cuts at: 2274 2303 3262 4056 4076 2274
Size: 29 959 794 20 3944
Fragments arranged by size:
3944 959 794 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2737 3193 4082
Size: 54 1425 50 275 18 907 456 889
Cuts at: 4082 5711 8
Size: 1629 43
Fragments arranged by size:
1629 1425 907 889 456 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 2387 3676 1353
Size: 933 101 1289 3423
Fragments arranged by size:
3423 1289 933 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2371 2965 3809 4406 5193 5461
Size: 393 635 104 594 844 597 787 268
Cuts at: 5461 1239
Size: 1524
Fragments arranged by size:
1524 844 787 635 597 594 393 268
104
EcoNI CCTnn'n_nnAGG
Cuts at: 2472 2472
Size: 5746
EcoO109I rG'GnC_Cy
Cuts at: 2010 2010
Size: 5746
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 5746
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 2022 2356 4893 1678
Size: 344 334 2537 2531
Fragments arranged by size:
2537 2531 344 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 4387 4419 4461
Size: 32 135 32 609 32 2174 32 42
Cuts at: 4461 4493 1373
Size: 32 2626
Fragments arranged by size:
2626 2174 609 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 2062 2607 3071 2062
Size: 545 464 4737
Fragments arranged by size:
4737 545 464
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2617 2908 4860 5044 1305
Size: 1312 291 1952 184 2007
Fragments arranged by size:
2007 1952 1312 291 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2739 10
Size: 54 1425 343 907 3017
Fragments arranged by size:
3017 1425 907 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2757 2885 3704 4063 4222 518
Size: 648 1591 128 819 359 159 2042
Fragments arranged by size:
2042 1591 819 648 359 159 128
MspA1I CmG'CkG
Cuts at: 1606 2170 2293 2528 3162 3839 4987 5232 1606
Size: 564 123 235 634 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 634 564 245 235 123
NcoI C'CATG_G
Cuts at: 513 1065 2004 2700 2880 513
Size: 552 939 696 180 3379
Fragments arranged by size:
3379 939 696 552 180
NdeI CA'TA_TG
Cuts at: 387 2770 387
Size: 2383 3363
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NsiI A_TGCA'T
Cuts at: 2463 2463
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PacI TTA_AT'TAA
Cuts at: 3042 3042
Size: 5746
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2792 1108
Size: 1684 4062
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 2234 3316 2234
Size: 1082 4664
PspOMI G'GGCC_C
Cuts at: 2010 2010
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2170 2528 2170
Size: 358 5388
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SapI GCTCTTCn'nnn_
Cuts at: 2286 2387 2286
Size: 101 5645
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 3094 4229 4910 5101 826
Size: 219 2049 1135 681 191 1471
Fragments arranged by size:
2049 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 2015 3073 1872
Size: 143 1058 4545
Fragments arranged by size:
4545 1058 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3850 4751 5013 820
Size: 197 926 1907 901 262 1553
Fragments arranged by size:
1907 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3000 3670 5
Size: 47 2948 670 2081
Fragments arranged by size:
2948 2081 670 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2004 2700 2880 3185 513
Size: 552 939 696 180 305 3074
Fragments arranged by size:
3074 939 696 552 305 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2605 3773 3790 3943 4128 4573 325
Size: 1255 1025 1168 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1168 1025 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3992 5704 96
Size: 274 80 33 51 1443 2015 1712 138
Fragments arranged by size:
2015 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 5706
Size: 32 5714
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 2013 3071 1870
Size: 143 1058 4545
Fragments arranged by size:
4545 1058 143
XmnI GAAnn'nnTTC
Cuts at: 2254 3875 2254
Size: 1621 4125
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI