Restriction map for
pFN21AE6301
(Circular) (Six-base) MAPSORT of: pFN21AE6301.seq Check: 6564 from: 1 to: 5974
pFN21AE6301 5974 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2901 3317 1937
Size: 964 416 4594
Fragments arranged by size:
4594 964 416
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 2701 2753 4037 5421 5689 1632
Size: 848 221 52 1284 1384 268 1917
Fragments arranged by size:
1917 1384 1284 848 268 221 52
AfeI AGC'GCT
Cuts at: 2261 2390 2261
Size: 129 5845
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 3143 1166
Size: 1977 3997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 2251 3717 5289 2251
Size: 1466 1572 2936
Fragments arranged by size:
2936 1572 1466
ApaI G_GGCC'C
Cuts at: 2477 2477
Size: 5974
ApaLI G'TGCA_C
Cuts at: 2426 4031 5187 2426
Size: 1605 1156 3213
Fragments arranged by size:
3213 1605 1156
ApoI r'AATT_y
Cuts at: 1493 2968 3283 3509 3661 5770 1493
Size: 1475 315 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 315 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 1870
Size: 73 1356 4545
Fragments arranged by size:
4545 1356 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2510 2543 2510
Size: 33 5941
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2477 3084 3293 1391
Size: 1086 607 209 4072
Fragments arranged by size:
4072 1086 607 209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2156 928
Size: 971 257 4746
Fragments arranged by size:
4746 971 257
BbvCI CC'TCA_GC
Cuts at: 2346 2346
Size: 5974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 3033 4585
Size: 107 122 71 631 605 685 676 1552
Cuts at: 4585 136
Size: 1525
Fragments arranged by size:
1552 1525 685 676 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3023 3391 3023
Size: 368 5606
Bme1580I G_kGCm'C
Cuts at: 1790 2430 2439 2477 3093 4035 5191 1790
Size: 640 9 38 616 942 1156 2573
Fragments arranged by size:
2573 1156 942 640 616 38 9
BmgBI CAC'GTC
Cuts at: 1318 3250 1318
Size: 1932 4042
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2242 2249 3705
Size: 416 53 83 399 24 825 7 1456
Cuts at: 3705 4663 442
Size: 958 1753
Fragments arranged by size:
1753 1456 958 825 416 399 83 53
24 7
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2047 2624 4634 1239
Size: 791 17 577 2010 2579
Fragments arranged by size:
2579 2010 791 577 17
Bpu10I CC'TnA_GC
Cuts at: 2346 2346
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4099 4964 5262 4099
Size: 865 298 4811
Fragments arranged by size:
4811 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2127 2501 2600 4637 882
Size: 617 628 374 99 2037 2219
Fragments arranged by size:
2219 2037 628 617 374 99
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2328 3216 4407 4805 5079 5226 5637 2328
Size: 888 1191 398 274 147 411 2665
Fragments arranged by size:
2665 1191 888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 3176 3191 1186
Size: 847 1143 15 3969
Fragments arranged by size:
3969 1143 847 15
BseYI C'CCAG_C
Cuts at: 2829 3228 5177 5673 2829
Size: 399 1949 496 3130
Fragments arranged by size:
3130 1949 496 399
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2430 4035 4120 5191 2430
Size: 1605 85 1071 3213
Fragments arranged by size:
3213 1605 1071 85
BsmI GAATG_Cn'
Cuts at: 2554 3485 3578 2554
Size: 931 93 4950
Fragments arranged by size:
4950 931 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2702 2702
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 2439 2477 3084 3093 3293 4035
Size: 399 640 9 38 607 9 200 742
Cuts at: 4035 4120 5191 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 640 607 399 200 85
38 9 9
BspEI T'CCGG_A
Cuts at: 3216 5637 3216
Size: 2421 3553
BspHI T'CATG_A
Cuts at: 2337 3863 5593 2337
Size: 1526 1730 2718
Fragments arranged by size:
2718 1730 1526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3022 3861 1394
Size: 429 1199 839 3507
Fragments arranged by size:
3507 1199 839 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2292 2703 4618 4805 1700
Size: 110 482 411 1915 187 2869
Fragments arranged by size:
2869 1915 482 411 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 2407 4034 5046 2407
Size: 1627 1012 3335
Fragments arranged by size:
3335 1627 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2434 2656 794
Size: 1640 222 4112
Fragments arranged by size:
4112 1640 222
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 2198 2845 3236 2198
Size: 647 391 4936
Fragments arranged by size:
4936 647 391
BstYI r'GATC_y
Cuts at: 1972 3304 3671 4056 4073 5525 5611 5623 5969
Size: 1332 367 385 17 1452 86 12 346
Cuts at: 5969 1972
Size: 1977
Fragments arranged by size:
1977 1452 1332 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2128 513
Size: 552 939 124 4359
Fragments arranged by size:
4359 939 552 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 3053 3490 4284 4304 3053
Size: 437 794 20 4723
Fragments arranged by size:
4723 794 437 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DraIII CAC_nnn'GTG
Cuts at: 2434 2434
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 3017 3421
Size: 54 1425 50 275 18 741 446 404
Cuts at: 3421 4310 5939 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 741 446 404 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2372 2639 2738 3185 3218 3904 1353
Size: 1019 267 99 447 33 686 3423
Fragments arranged by size:
3423 1019 686 447 267 99 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2768 4561 4935 5081 387
Size: 1064 138 1179 1793 374 146 1280
Fragments arranged by size:
1793 1280 1179 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2047 2480 2624 2701 2753 4037
Size: 393 398 17 433 144 77 52 1284
Cuts at: 4037 4634 5421 5689 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1284 787 597 433 398 393 268
144 77 52 17
EcoO109I rG'GnC_Cy
Cuts at: 2164 2248 3010 2164
Size: 84 762 5128
Fragments arranged by size:
5128 762 84
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FspI TGC'GCA
Cuts at: 2859 4480 2859
Size: 1621 4353
HaeII r_GCGC'y
Cuts at: 1678 2263 2392 5121 1678
Size: 585 129 2729 2531
Fragments arranged by size:
2729 2531 585 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4615 4647 4689 4721 1373
Size: 32 135 32 3043 32 42 32 2626
Fragments arranged by size:
3043 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2208 3318 3564 669
Size: 988 281 270 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 270 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 2208 3564 2208
Size: 1356 4618
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2573 10
Size: 54 1425 343 741 3411
Fragments arranged by size:
3411 1425 741 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3143 3932 4291 4450 518
Size: 648 1977 789 359 159 2042
Fragments arranged by size:
2042 1977 789 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3048 3118 3390 4067 5215 5460 1606
Size: 1442 70 272 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 272 245 70
NaeI GCC'GGC
Cuts at: 2705 2705
Size: 5974
NcoI C'CATG_G
Cuts at: 513 1065 2004 2128 513
Size: 552 939 124 4359
Fragments arranged by size:
4359 939 552 124
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NgoMIV G'CCGG_C
Cuts at: 2703 2703
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3332 4877 1132
Size: 97 2103 1545 2229
Fragments arranged by size:
2229 2103 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2191 2670 1348
Size: 843 479 4652
Fragments arranged by size:
4652 843 479
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3099 1108
Size: 1991 3983
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2164
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 2473 2473
Size: 5974
PstI C_TGCA'G
Cuts at: 830 3060 3136 3326 830
Size: 2230 76 190 3478
Fragments arranged by size:
3478 2230 190 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 3118 3118
Size: 5974
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SexAI A'CCwGG_T
Cuts at: 2067 2067
Size: 5974
SfcI C'TryA_G
Cuts at: 826 1045 2225 2498 3056 3132 3322 4457 5138
Size: 219 1180 273 558 76 190 1135 681
Cuts at: 5138 5329 826
Size: 191 1471
Fragments arranged by size:
1471 1180 1135 681 558 273 219 191
190 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4078 4979 5241 820
Size: 197 926 2135 901 262 1553
Fragments arranged by size:
2135 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 2128 2160 2211 3093 3156 3413
Size: 552 939 124 32 51 882 63 257
Cuts at: 3413 513
Size: 3074
Fragments arranged by size:
3074 939 882 552 257 124 63 51
32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 4001 4018 4171 4356 4801 325
Size: 1255 522 1899 17 153 185 445 1498
Fragments arranged by size:
1899 1498 1255 522 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2985 4220 5932
Size: 274 80 33 51 1443 1008 1235 1712
Cuts at: 5932 96
Size: 138
Fragments arranged by size:
1712 1443 1235 1008 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2986 3018 5934 5966 2986
Size: 32 2916 32 2994
Fragments arranged by size:
2994 2916 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2960 3254 2960
Size: 294 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI