Restriction map for
pFN21AE6296
(Circular) (Six-base) MAPSORT of: pFN21AE6296.seq Check: 7258 from: 1 to: 5254
pFN21AE6296 5254 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2017 2017
Size: 5254
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3064 278
Size: 53 83 186 936 1528 2468
Fragments arranged by size:
2468 1528 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2597 1937
Size: 660 4594
Acc65I G'GTAC_C
Cuts at: 2575 2575
Size: 5254
AclI AA'CG_TT
Cuts at: 1563 3381 3754 1563
Size: 1818 373 3063
Fragments arranged by size:
3063 1818 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3317 4701 4969 1632
Size: 1685 1384 268 1917
Fragments arranged by size:
1917 1685 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5057
AflIII A'CryG_T
Cuts at: 1203 4153 5130 1203
Size: 2950 977 1327
Fragments arranged by size:
2950 1327 977
AgeI A'CCGG_T
Cuts at: 4085 4085
Size: 5254
AhdI GACnn_n'nnGTC
Cuts at: 3983 3983
Size: 5254
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5254
AlwNI CAG_nnn'CTG
Cuts at: 2212 2230 2347 2997 4569 2212
Size: 18 117 650 1572 2897
Fragments arranged by size:
2897 1572 650 117 18
ApaLI G'TGCA_C
Cuts at: 3311 4467 3311
Size: 1156 4098
ApoI r'AATT_y
Cuts at: 1493 2563 2789 2941 5050 1493
Size: 1070 226 152 2109 1697
Fragments arranged by size:
2109 1697 1070 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3808 160
Size: 872 2776 1606
Fragments arranged by size:
2776 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5254
AvaI C'yCGr_G
Cuts at: 1870 1943 2579 1870
Size: 73 636 4545
Fragments arranged by size:
4545 636 73
BamHI G'GATC_C
Cuts at: 2584 2951 2584
Size: 367 4887
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2575 4030 618
Size: 325 217 627 788 1455 1842
Fragments arranged by size:
1842 1455 788 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2298 2573 1391
Size: 700 207 275 4072
Fragments arranged by size:
4072 700 275 207
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3445 3479 1903
Size: 34 1508 34 3678
Fragments arranged by size:
3678 1508 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3146 4356 3146
Size: 1210 4044
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5254
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2501 3865 136
Size: 107 122 71 631 605 829 1364 1525
Fragments arranged by size:
1525 1364 829 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5249 5249
Size: 5254
BlpI GC'TnA_GC
Cuts at: 2671 2671
Size: 5254
Bme1580I G_kGCm'C
Cuts at: 1790 3315 4471 1790
Size: 1525 1156 2573
Fragments arranged by size:
2573 1525 1156
BmgBI CAC'GTC
Cuts at: 1318 2143 1318
Size: 825 4429
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2985 3943 442
Size: 416 53 83 399 24 1568 958 1753
Fragments arranged by size:
1753 1568 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2171 2345 2426 3914 1239
Size: 866 66 174 81 1488 2579
Fragments arranged by size:
2579 1488 866 174 81 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3379 4244 4542 3379
Size: 865 298 4091
Fragments arranged by size:
4091 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3917 882
Size: 617 2418 2219
Fragments arranged by size:
2418 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2461 3045 493
Size: 711 1257 584 2702
Fragments arranged by size:
2702 1257 711 584
BsaBI GATnn'nnATC
Cuts at: 5248 5248
Size: 5254
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 3061 3443 4078
Size: 53 83 186 936 525 1003 382 635
Cuts at: 4078 275
Size: 1451
Fragments arranged by size:
1451 1003 936 635 525 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3687 4085 4359 4506 4917 3687
Size: 398 274 147 411 4024
Fragments arranged by size:
4024 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5029
Fragments arranged by size:
5029 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5254
BseYI C'CCAG_C
Cuts at: 2016 2283 2388 2449 4457 4953 2016
Size: 267 105 61 2008 496 2317
Fragments arranged by size:
2317 2008 496 267 105 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 1163
Size: 573 669 4012
Fragments arranged by size:
4012 669 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2539 2704 3465 4493 1540
Size: 275 186 538 165 761 1028 2301
Fragments arranged by size:
2301 1028 761 538 275 186 165
BsiHKAI G_wGCw'C
Cuts at: 2091 3315 3400 4471 2091
Size: 1224 85 1071 2874
Fragments arranged by size:
2874 1224 1071 85
BsmI GAATG_Cn'
Cuts at: 2765 2858 2765
Size: 93 5161
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2298 2573 3315 3400 4471 1391
Size: 399 301 207 275 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 301 275 207 85
BspEI T'CCGG_A
Cuts at: 4917 4917
Size: 5254
BspHI T'CATG_A
Cuts at: 3143 4873 3143
Size: 1730 3524
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2017 2238 2427 2478 2609 844
Size: 1173 221 189 51 131 3489
Fragments arranged by size:
3489 1173 221 189 131 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2292 3141 1394
Size: 429 469 849 3507
Fragments arranged by size:
3507 849 469 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3749 3923 65
Size: 1183 2501 174 1396
Fragments arranged by size:
2501 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2495 3898 4085 1700
Size: 110 685 1403 187 2869
Fragments arranged by size:
2869 1403 685 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5254
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5254
BssSI C'ACGA_G
Cuts at: 2297 2423 3314 4326 2297
Size: 126 891 1012 3225
Fragments arranged by size:
3225 1012 891 126
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2230 794
Size: 1436 3818
BstBI TT'CG_AA
Cuts at: 4058 4058
Size: 5254
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5254
BstYI r'GATC_y
Cuts at: 1972 2353 2584 2951 3336 3353 4805 4891 4903
Size: 381 231 367 385 17 1452 86 12
Cuts at: 4903 5249 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 381 367 346 231 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2304 2370 513
Size: 552 939 300 66 3397
Fragments arranged by size:
3397 939 552 300 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4515
Fragments arranged by size:
4515 683 56
BtsI GCAGTG_nn'
Cuts at: 2770 3564 3584 2770
Size: 794 20 4440
Fragments arranged by size:
4440 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5254
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5219
DraI TTT'AAA
Cuts at: 2558 2558
Size: 5254
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4261 809
Size: 507 2945 1802
Fragments arranged by size:
2945 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2239 2301
Size: 54 1425 50 275 18 177 232 62
Cuts at: 2301 2367 2536 2701 3590 5219 8
Size: 66 169 165 889 1629 43
Fragments arranged by size:
1629 1425 889 275 232 177 169 165
66 62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2536 2701 1537
Size: 275 724 165 4090
Fragments arranged by size:
4090 724 275 165
EarI CTCTTCn'nnn_
Cuts at: 1353 3184 1353
Size: 1831 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2302 3841 4215 4361 387
Size: 1064 138 713 1539 374 146 1280
Fragments arranged by size:
1539 1280 1064 713 374 146 138
EcoICRI GAG'CTC
Cuts at: 2089 2089
Size: 5254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2171 2345 2426 3317 3914 4701
Size: 393 473 66 174 81 891 597 787
Cuts at: 4701 4969 1239
Size: 268 1524
Fragments arranged by size:
1524 891 787 597 473 393 268 174
81 66
EcoRI G'AATT_C
Cuts at: 2563 2941 2563
Size: 378 4876
EcoRV GAT'ATC
Cuts at: 2260 2260
Size: 5254
FspI TGC'GCA
Cuts at: 2474 3760 2474
Size: 1286 3968
HaeII r_GCGC'y
Cuts at: 1678 4401 1678
Size: 2723 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3895 3927 3969 4001 1373
Size: 32 135 32 2323 32 42 32 2626
Fragments arranged by size:
2626 2323 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 2598 2844 669
Size: 988 281 400 260 246 3079
Fragments arranged by size:
3079 988 400 281 260 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5254
HpaI GTT'AAC
Cuts at: 2844 2844
Size: 5254
KpnI G_GTAC'C
Cuts at: 2579 2579
Size: 5254
MfeI C'AATT_G
Cuts at: 2853 2853
Size: 5254
MluI A'CGCG_T
Cuts at: 5130 5130
Size: 5254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 4368 4552 1305
Size: 858 2205 184 2007
Fragments arranged by size:
2205 2007 858 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2241 2303 10
Size: 54 1425 343 177 232 62 2961
Fragments arranged by size:
2961 1425 343 232 177 62 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3212 3571 3730 518
Size: 648 2046 359 159 2042
Fragments arranged by size:
2046 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2365 2392 2670 3347 4495 4740 1606
Size: 759 27 278 677 1148 245 2120
Fragments arranged by size:
2120 1148 759 677 278 245 27
NaeI GCC'GGC
Cuts at: 2497 2497
Size: 5254
NcoI C'CATG_G
Cuts at: 513 1065 2004 2304 513
Size: 552 939 300 3463
Fragments arranged by size:
3463 939 552 300
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5254
NgoMIV G'CCGG_C
Cuts at: 2495 2495
Size: 5254
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5254
NotI GC'GGCC_GC
Cuts at: 2536 2701 2536
Size: 165 5089
NspI r_CATG'y
Cuts at: 1132 1229 2204 2612 4157 1132
Size: 97 975 408 1545 2229
Fragments arranged by size:
2229 1545 975 408 97
PasI CC'CwG_GG
Cuts at: 1348 2530 1348
Size: 1182 4072
PciI A'CATG_T
Cuts at: 4153 4153
Size: 5254
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2404 1108
Size: 1296 3958
PfoI T'CCnGG_A
Cuts at: 2403 2403
Size: 5254
PmeI GTTT'AAAC
Cuts at: 2558 2558
Size: 5254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2498 2530 3323 3355 4836 4868 2498
Size: 32 793 32 1481 32 2884
Fragments arranged by size:
2884 1481 793 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5254
PsiI TTA'TAA
Cuts at: 2824 2824
Size: 5254
PstI C_TGCA'G
Cuts at: 830 2226 2606 830
Size: 1396 380 3478
Fragments arranged by size:
3478 1396 380
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5254
PvuII CAG'CTG
Cuts at: 2365 2392 2365
Size: 27 5227
SacI G_AGCT'C
Cuts at: 2091 2091
Size: 5254
SalI G'TCGA_C
Cuts at: 1936 2596 1936
Size: 660 4594
SbfI CC_TGCA'GG
Cuts at: 2606 2606
Size: 5254
ScaI AGT'ACT
Cuts at: 3502 5214 3502
Size: 1712 3542
SfcI C'TryA_G
Cuts at: 826 1045 2222 2602 3737 4418 4609 826
Size: 219 1177 380 1135 681 191 1471
Fragments arranged by size:
1471 1177 1135 681 380 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5254
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5254
SmaI CCC'GGG
Cuts at: 1872 2581 1872
Size: 709 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3358 4259 4521 820
Size: 197 926 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3045 493
Size: 2552 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5254
SphI G_CATG'C
Cuts at: 1132 2204 2612 1132
Size: 1072 408 3774
Fragments arranged by size:
3774 1072 408
SspI AAT'ATT
Cuts at: 5 52 3178 5
Size: 47 3126 2081
Fragments arranged by size:
3126 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5254
StyI C'CwwG_G
Cuts at: 513 1065 2004 2278 2304 2693 513
Size: 552 939 274 26 389 3074
Fragments arranged by size:
3074 939 552 389 274 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2441 3281 3298 3451 3636 4081 325
Size: 1255 861 840 17 153 185 445 1498
Fragments arranged by size:
1498 1255 861 840 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3500 5212 96
Size: 274 80 33 51 1443 1523 1712 138
Fragments arranged by size:
1712 1523 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5214 5246 5214
Size: 32 5222
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5254
XbaI T'CTAG_A
Cuts at: 2590 2590
Size: 5254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 2446
Size: 5254
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5254
XmaI C'CCGG_G
Cuts at: 1870 2579 1870
Size: 709 4545
XmnI GAAnn'nnTTC
Cuts at: 2159 3383 2159
Size: 1224 4030
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3062 276
Size: 53 83 186 936 1528 2468
Fragments arranged by size:
2468 1528 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI