Restriction map for
pFN21AE6293
(Circular) (Six-base) MAPSORT of: pFN21AE6293.seq Check: 8568 from: 1 to: 5437
pFN21AE6293 5437 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2411 2411
Size: 5437
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 1937
Size: 843 4594
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3500 4884 5152 1632
Size: 1868 1384 268 1917
Fragments arranged by size:
1917 1868 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 2177 4268 2177
Size: 2091 3346
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5437
AlwNI CAG_nnn'CTG
Cuts at: 3180 4752 3180
Size: 1572 3865
ApaI G_GGCC'C
Cuts at: 2717 2717
Size: 5437
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2183 2288 2746 2972 3124 5233 1493
Size: 690 105 458 226 152 2109 1697
Fragments arranged by size:
2109 1697 690 458 226 152 105
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2489 2592 2726 2762 1870
Size: 73 546 103 134 36 4545
Fragments arranged by size:
4545 546 134 103 73 36
BamHI G'GATC_C
Cuts at: 2258 2767 3134 2258
Size: 509 367 4561
Fragments arranged by size:
4561 509 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2497 2758 4213 618
Size: 325 217 627 710 261 1455 1842
Fragments arranged by size:
1842 1455 710 627 325 261 217
BanII G_rGCy'C
Cuts at: 1391 2488 2717 2756 1391
Size: 1097 229 39 4072
Fragments arranged by size:
4072 1097 229 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2199 2540 928
Size: 971 300 341 3825
Fragments arranged by size:
3825 971 341 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2347 2381 3628 3662 1903
Size: 34 410 34 1247 34 3678
Fragments arranged by size:
3678 1247 410 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4539 3329
Size: 1210 4227
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4048 136
Size: 107 122 71 631 605 2376 1525
Fragments arranged by size:
2376 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2538 5432 2538
Size: 2894 2543
BlpI GC'TnA_GC
Cuts at: 2854 2854
Size: 5437
Bme1580I G_kGCm'C
Cuts at: 1790 2502 2717 3498 4654 1790
Size: 712 215 781 1156 2573
Fragments arranged by size:
2573 1156 781 712 215
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3168 4126 442
Size: 416 53 83 399 24 1751 958 1753
Fragments arranged by size:
1753 1751 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4097 1239
Size: 2858 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2688 4100 882
Size: 617 1189 1412 2219
Fragments arranged by size:
2219 1412 1189 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3244 3626 4261 275
Size: 53 83 186 936 1711 382 635 1451
Fragments arranged by size:
1711 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2177 3870 4268 4542 4689 5100 2177
Size: 1693 398 274 147 411 2514
Fragments arranged by size:
2514 1693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5212
Fragments arranged by size:
5212 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5437
BseYI C'CCAG_C
Cuts at: 2128 2502 4640 5136 2128
Size: 374 2138 496 2429
Fragments arranged by size:
2429 2138 496 374
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2439 1163
Size: 573 703 4161
Fragments arranged by size:
4161 703 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2887 3648 4676 1540
Size: 275 186 886 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 3498 3583 4654 2601
Size: 897 85 1071 3384
Fragments arranged by size:
3384 1071 897 85
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2488 2502 2601 2717 2756 3498 3583
Size: 399 698 14 99 116 39 742 85
Cuts at: 3583 4654 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 698 399 116 99 85
39 14
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2411 2484 2792 844
Size: 1567 73 308 3489
Fragments arranged by size:
3489 1567 308 73
BsrBI CCG'CTC
Cuts at: 1394 1823 3324 1394
Size: 429 1501 3507
Fragments arranged by size:
3507 1501 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3932 4106 65
Size: 1183 2684 174 1396
Fragments arranged by size:
2684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2177 4081 4268 1700
Size: 110 367 1904 187 2869
Fragments arranged by size:
2869 1904 367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5437
BstXI CCAn_nnnn'nTGG
Cuts at: 2404 2543 2404
Size: 139 5298
BstYI r'GATC_y
Cuts at: 1972 2258 2538 2767 3134 3519 3536 4988 5074
Size: 286 280 229 367 385 17 1452 86
Cuts at: 5074 5086 5432 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 286 280 229
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2132 2953 3747 3767 2132
Size: 821 794 20 3802
Fragments arranged by size:
3802 821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2359 2394 2605 2640 529
Size: 35 1795 35 211 35 3326
Fragments arranged by size:
3326 1795 211 35 35 35
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4444 809
Size: 507 3128 1802
Fragments arranged by size:
3128 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2884 3773 5402
Size: 54 1425 50 275 18 1054 889 1629
Cuts at: 5402 8
Size: 43
Fragments arranged by size:
1629 1425 1054 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2884 1537
Size: 275 1072 4090
Fragments arranged by size:
4090 1072 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 2691 3367 1353
Size: 1160 178 676 3423
Fragments arranged by size:
3423 1160 676 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3500 4097 4884 5152 1239
Size: 393 1868 597 787 268 1524
Fragments arranged by size:
1868 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2304 2713 2304
Size: 409 5028
EcoRI G'AATT_C
Cuts at: 2183 2746 3124 2183
Size: 563 378 4496
Fragments arranged by size:
4496 563 378
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 4584 1678
Size: 2906 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2238 2270 2530 2562 4078
Size: 32 135 32 666 32 260 32 1516
Cuts at: 4078 4110 4152 4184 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1516 666 260 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2122 2781 3027 669
Size: 988 281 184 659 246 3079
Fragments arranged by size:
3079 988 659 281 246 184
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2457 4551 4735 1305
Size: 1152 2094 184 2007
Fragments arranged by size:
2094 2007 1152 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3615
Fragments arranged by size:
3615 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 3395 3754 3913 518
Size: 648 1362 867 359 159 2042
Fragments arranged by size:
2042 1362 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2853 3530 4678 4923 1606
Size: 1247 677 1148 245 2120
Fragments arranged by size:
2120 1247 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2795 4340 1132
Size: 97 1566 1545 2229
Fragments arranged by size:
2229 1566 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5437
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PspOMI G'GGCC_C
Cuts at: 2713 2713
Size: 5437
PstI C_TGCA'G
Cuts at: 830 2677 2789 830
Size: 1847 112 3478
Fragments arranged by size:
3478 1847 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SapI GCTCTTCn'nnn_
Cuts at: 2513 2513
Size: 5437
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SfcI C'TryA_G
Cuts at: 826 1045 2673 2785 3920 4601 4792 826
Size: 219 1628 112 1135 681 191 1471
Fragments arranged by size:
1628 1471 1135 681 219 191 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2764 1872
Size: 892 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4442 4704 820
Size: 197 926 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5437
StyI C'CwwG_G
Cuts at: 513 1065 2004 2277 2623 2706 2876 513
Size: 552 939 273 346 83 170 3074
Fragments arranged by size:
3074 939 552 346 273 170 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3464 3481 3634 3819 4264 325
Size: 1255 1884 17 153 185 445 1498
Fragments arranged by size:
1884 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 3683 5395
Size: 274 80 33 51 1443 471 1235 1712
Cuts at: 5395 96
Size: 138
Fragments arranged by size:
1712 1443 1235 471 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2404 2404
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2762 1870
Size: 892 4545
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI