Restriction map for
pFN21AE6283
(Circular) (Six-base) MAPSORT of: pFN21AE6283.seq Check: 9368 from: 1 to: 5509
pFN21AE6283 5509 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3319 278
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2852 1937
Size: 915 4594
Acc65I G'GTAC_C
Cuts at: 2384 2830 2384
Size: 446 5063
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2322 2529 3572 4956 5224 1632
Size: 690 207 1043 1384 268 1917
Fragments arranged by size:
1917 1384 1043 690 268 207
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 4408 5385 1203
Size: 3205 977 1327
Fragments arranged by size:
3205 1327 977
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 4238 4238
Size: 5509
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5509
AlwNI CAG_nnn'CTG
Cuts at: 2333 3252 4824 2333
Size: 919 1572 3018
Fragments arranged by size:
3018 1572 919
ApaLI G'TGCA_C
Cuts at: 3566 4722 3566
Size: 1156 4353
ApoI r'AATT_y
Cuts at: 1493 2818 3044 3196 5305 1493
Size: 1325 226 152 2109 1697
Fragments arranged by size:
2109 1697 1325 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4063 160
Size: 872 3031 1606
Fragments arranged by size:
3031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2834 1870
Size: 73 127 764 4545
Fragments arranged by size:
4545 764 127 73
BamHI G'GATC_C
Cuts at: 2839 3206 2839
Size: 367 5142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2350 2384 2636 2830 4285
Size: 325 217 627 563 34 252 194 1455
Cuts at: 4285 618
Size: 1842
Fragments arranged by size:
1842 1455 627 563 325 252 217 194
34
BanII G_rGCy'C
Cuts at: 1391 2012 2135 2507 2828 1391
Size: 621 123 372 321 4072
Fragments arranged by size:
4072 621 372 321 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 2310 928
Size: 971 347 64 4127
Fragments arranged by size:
4127 971 347 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2051 2085 3700 3734 1903
Size: 34 114 34 1615 34 3678
Fragments arranged by size:
3678 1615 114 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3401 4611 3401
Size: 1210 4299
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4120 136
Size: 107 122 71 631 605 2448 1525
Fragments arranged by size:
2448 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2540 5504 2540
Size: 2964 2545
BlpI GC'TnA_GC
Cuts at: 2011 2926 2011
Size: 915 4594
Bme1580I G_kGCm'C
Cuts at: 1790 3570 4726 1790
Size: 1780 1156 2573
Fragments arranged by size:
2573 1780 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2021 2383 3240
Size: 416 53 83 399 24 604 362 857
Cuts at: 3240 4198 442
Size: 958 1753
Fragments arranged by size:
1753 958 857 604 416 399 362 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5509
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 5509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2760 4169 1239
Size: 950 571 1409 2579
Fragments arranged by size:
2579 1409 950 571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2503 3634 4499 4797 2503
Size: 1131 865 298 3215
Fragments arranged by size:
3215 1131 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2247 4172 882
Size: 617 748 1925 2219
Fragments arranged by size:
2219 1925 748 617
BsaAI yAC'GTr
Cuts at: 493 1204 3300 493
Size: 711 2096 2702
Fragments arranged by size:
2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3316 3698 4333 275
Size: 53 83 186 936 1783 382 635 1451
Fragments arranged by size:
1783 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3942 4340 4614 4761 5172 3942
Size: 398 274 147 411 4279
Fragments arranged by size:
4279 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5284
Fragments arranged by size:
5284 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5509
BseYI C'CCAG_C
Cuts at: 4712 5208 4712
Size: 496 5013
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4936
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2959 3720 4748 1540
Size: 275 186 958 761 1028 2301
Fragments arranged by size:
2301 1028 958 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2135 2507 3570 3655 4726 2135
Size: 372 1063 85 1071 2918
Fragments arranged by size:
2918 1071 1063 372 85
BsmI GAATG_Cn'
Cuts at: 3020 3113 3020
Size: 93 5416
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2135 2507 2828 3570 3655 4726
Size: 399 222 123 372 321 742 85 1071
Cuts at: 4726 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 372 321 222 123
85
BspEI T'CCGG_A
Cuts at: 5172 5172
Size: 5509
BspHI T'CATG_A
Cuts at: 3398 5128 3398
Size: 1730 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2864 844
Size: 2020 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2205 3396 1394
Size: 429 382 1191 3507
Fragments arranged by size:
3507 1191 429 382
BsrDI GCAATG_nn'
Cuts at: 65 1248 4004 4178 65
Size: 1183 2756 174 1396
Fragments arranged by size:
2756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4153 4340 1700
Size: 110 2343 187 2869
Fragments arranged by size:
2869 2343 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5509
BssSI C'ACGA_G
Cuts at: 3569 4581 3569
Size: 1012 4497
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5509
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstXI CCAn_nnnn'nTGG
Cuts at: 2125 2125
Size: 5509
BstYI r'GATC_y
Cuts at: 1972 2197 2540 2597 2839 3206 3591 3608 5060
Size: 225 343 57 242 367 385 17 1452
Cuts at: 5060 5146 5158 5504 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 343 242 225
86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4018
Fragments arranged by size:
4018 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4770
Fragments arranged by size:
4770 683 56
BtsI GCAGTG_nn'
Cuts at: 2254 3025 3819 3839 2254
Size: 771 794 20 3924
Fragments arranged by size:
3924 794 771 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2813 2813
Size: 5509
DraIII CAC_nnn'GTG
Cuts at: 2096 2096
Size: 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4516 809
Size: 507 3200 1802
Fragments arranged by size:
3200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2493 2956 3845
Size: 54 1425 50 275 18 663 463 889
Cuts at: 3845 5474 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 663 463 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2956 1537
Size: 275 1144 4090
Fragments arranged by size:
4090 1144 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3439 1353
Size: 2086 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2037 2093 4096 4470 4616 387
Size: 1064 138 448 56 2003 374 146 1280
Fragments arranged by size:
2003 1280 1064 448 374 146 138 56
EcoICRI GAG'CTC
Cuts at: 2133 2505 2133
Size: 372 5137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2322 2529 2760 3572 4169 4956
Size: 393 557 133 207 231 812 597 787
Cuts at: 4956 5224 1239
Size: 268 1524
Fragments arranged by size:
1524 812 787 597 557 393 268 231
207 133
EcoO109I rG'GnC_Cy
Cuts at: 2524 2524
Size: 5509
EcoRI G'AATT_C
Cuts at: 2818 3196 2818
Size: 378 5131
FspI TGC'GCA
Cuts at: 2111 2558 4015 2111
Size: 447 1457 3605
Fragments arranged by size:
3605 1457 447
HaeII r_GCGC'y
Cuts at: 1678 4656 1678
Size: 2978 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 2297 2329 2501
Size: 32 135 32 617 32 76 32 172
Cuts at: 2501 2533 2589 2612 2621 2644 4150 4182 4224
Size: 32 56 23 9 23 1506 32 42
Cuts at: 4224 4256 1373
Size: 32 2626
Fragments arranged by size:
2626 1506 617 172 135 76 56 42
32 32 32 32 32 32 32 23
23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2853 3099 669
Size: 988 281 915 246 3079
Fragments arranged by size:
3079 988 915 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5509
HpaI GTT'AAC
Cuts at: 3099 3099
Size: 5509
KpnI G_GTAC'C
Cuts at: 2388 2834 2388
Size: 446 5063
MfeI C'AATT_G
Cuts at: 3108 3108
Size: 5509
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2532 4623 4807 1305
Size: 1227 2091 184 2007
Fragments arranged by size:
2091 2007 1227 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2495 10
Size: 54 1425 343 663 3024
Fragments arranged by size:
3024 1425 663 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 3467 3826 3985 518
Size: 648 957 1344 359 159 2042
Fragments arranged by size:
2042 1344 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2281 2925 3602 4750 4995 1606
Size: 675 644 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 644 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4018
Fragments arranged by size:
4018 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5509
NotI GC'GGCC_GC
Cuts at: 2956 2956
Size: 5509
NspI r_CATG'y
Cuts at: 1132 1229 2186 2867 4412 1132
Size: 97 957 681 1545 2229
Fragments arranged by size:
2229 1545 957 681 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5509
PciI A'CATG_T
Cuts at: 4408 4408
Size: 5509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5509
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 5091 5123 3578
Size: 32 1481 32 3964
Fragments arranged by size:
3964 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2524 2524
Size: 5509
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5509
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PspXI vC'TCGA_Gb
Cuts at: 2070 2070
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2619 2861 830
Size: 1789 242 3478
Fragments arranged by size:
3478 1789 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
PvuII CAG'CTG
Cuts at: 2281 2281
Size: 5509
SacI G_AGCT'C
Cuts at: 2135 2507 2135
Size: 372 5137
SalI G'TCGA_C
Cuts at: 1936 2851 1936
Size: 915 4594
SanDI GG'GwC_CC
Cuts at: 2524 2524
Size: 5509
SbfI CC_TGCA'GG
Cuts at: 2619 2861 2619
Size: 242 5267
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2615 2857 3992 4673 4864 826
Size: 219 1570 242 1135 681 191 1471
Fragments arranged by size:
1570 1471 1135 681 242 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5509
SmaI CCC'GGG
Cuts at: 1872 2836 1872
Size: 964 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2070 2518 3613 4514 4776 820
Size: 197 926 127 448 1095 901 262 1553
Fragments arranged by size:
1553 1095 926 901 448 262 197 127
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2867 1132
Size: 1735 3774
SspI AAT'ATT
Cuts at: 5 52 3433 5
Size: 47 3381 2081
Fragments arranged by size:
3381 2081 47
StuI AGG'CCT
Cuts at: 1517 2148 1517
Size: 631 4878
StyI C'CwwG_G
Cuts at: 513 1065 2004 2417 2457 2796 2948 513
Size: 552 939 413 40 339 152 3074
Fragments arranged by size:
3074 939 552 413 339 152 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 3553 3706 3891 4336 325
Size: 1255 1956 17 153 185 445 1498
Fragments arranged by size:
1956 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3755 5467 96
Size: 274 80 33 51 1443 1778 1712 138
Fragments arranged by size:
1778 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5469 5501 5469
Size: 32 5477
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5509
XbaI T'CTAG_A
Cuts at: 2845 2845
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 2070 1943
Size: 127 5382
XmaI C'CCGG_G
Cuts at: 1870 2834 1870
Size: 964 4545
XmnI GAAnn'nnTTC
Cuts at: 3638 3638
Size: 5509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3317 276
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI