Restriction map for
pFN21AE6282
(Circular) (Six-base) MAPSORT of: pFN21AE6282.seq Check: 8837 from: 1 to: 5473
pFN21AE6282 5473 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3283 278
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 1937
Size: 879 4594
Acc65I G'GTAC_C
Cuts at: 2794 2794
Size: 5473
AclI AA'CG_TT
Cuts at: 1563 3600 3973 1563
Size: 2037 373 3063
Fragments arranged by size:
3063 2037 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 3536 4920 5188 1632
Size: 443 1461 1384 268 1917
Fragments arranged by size:
1917 1461 1384 443 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5276
AflIII A'CryG_T
Cuts at: 1203 4372 5349 1203
Size: 3169 977 1327
Fragments arranged by size:
3169 1327 977
AgeI A'CCGG_T
Cuts at: 4304 4304
Size: 5473
AhdI GACnn_n'nnGTC
Cuts at: 4202 4202
Size: 5473
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5473
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2415 1184
Size: 1231 4242
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2148 2180 2148
Size: 32 5441
AlwNI CAG_nnn'CTG
Cuts at: 2479 3216 4788 2479
Size: 737 1572 3164
Fragments arranged by size:
3164 1572 737
ApaLI G'TGCA_C
Cuts at: 3530 4686 3530
Size: 1156 4317
ApoI r'AATT_y
Cuts at: 1493 2189 2782 3008 3160 5269 1493
Size: 696 593 226 152 2109 1697
Fragments arranged by size:
2109 1697 696 593 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4027 160
Size: 872 2995 1606
Fragments arranged by size:
2995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2798 1870
Size: 73 70 785 4545
Fragments arranged by size:
4545 785 73 70
AvrII C'CTAG_G
Cuts at: 2071 2071
Size: 5473
BamHI G'GATC_C
Cuts at: 2803 3170 2803
Size: 367 5106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2794 4249 618
Size: 325 217 627 1007 1455 1842
Fragments arranged by size:
1842 1455 1007 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2792 1391
Size: 1401 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3664 3698 1903
Size: 34 50 34 1643 34 3678
Fragments arranged by size:
3678 1643 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3365 4575 3365
Size: 1210 4263
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 4084 136
Size: 107 122 71 631 605 370 2042 1525
Fragments arranged by size:
2042 1525 631 605 370 122 107 71
BglII A'GATC_T
Cuts at: 5468 5468
Size: 5473
BlpI GC'TnA_GC
Cuts at: 2890 2890
Size: 5473
Bme1580I G_kGCm'C
Cuts at: 1790 3534 4690 1790
Size: 1744 1156 2573
Fragments arranged by size:
2573 1744 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5473
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2256 3204 4162
Size: 416 53 83 399 24 839 948 958
Cuts at: 4162 442
Size: 1753
Fragments arranged by size:
1753 958 948 839 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4133 1239
Size: 2894 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2739 3598 4463 4761 2739
Size: 859 865 298 3451
Fragments arranged by size:
3451 865 859 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2291 4136 882
Size: 617 792 1845 2219
Fragments arranged by size:
2219 1845 792 617
BsaAI yAC'GTr
Cuts at: 493 1204 2671 3264 493
Size: 711 1467 593 2702
Fragments arranged by size:
2702 1467 711 593
BsaBI GATnn'nnATC
Cuts at: 5467 5467
Size: 5473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3280 3662 4297 275
Size: 53 83 186 936 1747 382 635 1451
Fragments arranged by size:
1747 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3906 4304 4578 4725 5136 3906
Size: 398 274 147 411 4243
Fragments arranged by size:
4243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2148 2178 1178
Size: 30 165 30 745 30 4473
Fragments arranged by size:
4473 745 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5473
BseYI C'CCAG_C
Cuts at: 4676 5172 4676
Size: 496 4977
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2633 1163
Size: 573 897 4003
Fragments arranged by size:
4003 897 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2923 3684 4712 1540
Size: 275 186 922 761 1028 2301
Fragments arranged by size:
2301 1028 922 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3534 3619 4690 3534
Size: 85 1071 4317
Fragments arranged by size:
4317 1071 85
BsmI GAATG_Cn'
Cuts at: 2065 2452 2984 3077 2065
Size: 387 532 93 4461
Fragments arranged by size:
4461 532 387 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2792 3534 3619 4690 1391
Size: 399 1002 742 85 1071 2174
Fragments arranged by size:
2174 1071 1002 742 399 85
BspEI T'CCGG_A
Cuts at: 5136 5136
Size: 5473
BspHI T'CATG_A
Cuts at: 3362 5092 3362
Size: 1730 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2828 844
Size: 1984 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3360 1394
Size: 429 1537 3507
Fragments arranged by size:
3507 1537 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 2721 3968 4142 65
Size: 1183 1275 198 1247 174 1396
Fragments arranged by size:
1396 1275 1247 1183 198 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4117 4304 1700
Size: 110 2307 187 2869
Fragments arranged by size:
2869 2307 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5473
BssSI C'ACGA_G
Cuts at: 3533 4545 3533
Size: 1012 4461
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2372 2674 794
Size: 1578 302 3593
Fragments arranged by size:
3593 1578 302
BstBI TT'CG_AA
Cuts at: 4277 4277
Size: 5473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5473
BstYI r'GATC_y
Cuts at: 1972 2803 3170 3555 3572 5024 5110 5122 5468
Size: 831 367 385 17 1452 86 12 346
Cuts at: 5468 1972
Size: 1977
Fragments arranged by size:
1977 1452 831 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2184 2184
Size: 5473
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4921
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4734
Fragments arranged by size:
4734 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 2989 3783 3803 2304
Size: 685 794 20 3974
Fragments arranged by size:
3974 794 685 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5438
DraI TTT'AAA
Cuts at: 2777 2777
Size: 5473
DraIII CAC_nnn'GTG
Cuts at: 2674 2674
Size: 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4480 809
Size: 507 3164 1802
Fragments arranged by size:
3164 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2920 3809 5438
Size: 54 1425 50 275 18 1090 889 1629
Cuts at: 5438 8
Size: 43
Fragments arranged by size:
1629 1425 1090 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2920 1537
Size: 275 1108 4090
Fragments arranged by size:
4090 1108 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 2406 3403 1353
Size: 795 258 997 3423
Fragments arranged by size:
3423 997 795 258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2017 4060 4434 4580 387
Size: 1064 138 428 2043 374 146 1280
Fragments arranged by size:
2043 1280 1064 428 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 3536 4133 4920 5188 1239
Size: 393 443 1461 597 787 268 1524
Fragments arranged by size:
1524 1461 787 597 443 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2100 2228 2100
Size: 128 5345
EcoRI G'AATT_C
Cuts at: 2189 2782 3160 2189
Size: 593 378 4502
Fragments arranged by size:
4502 593 378
FspI TGC'GCA
Cuts at: 3979 3979
Size: 5473
HaeII r_GCGC'y
Cuts at: 1678 2494 4620 1678
Size: 816 2126 2531
Fragments arranged by size:
2531 2126 816
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4114 4146 4188 4220 1373
Size: 32 135 32 2542 32 42 32 2626
Fragments arranged by size:
2626 2542 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2817 3063 669
Size: 988 281 879 246 3079
Fragments arranged by size:
3079 988 879 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5473
HpaI GTT'AAC
Cuts at: 3063 3063
Size: 5473
KpnI G_GTAC'C
Cuts at: 2798 2798
Size: 5473
MfeI C'AATT_G
Cuts at: 2451 2709 3072 2451
Size: 258 363 4852
Fragments arranged by size:
4852 363 258
MluI A'CGCG_T
Cuts at: 5349 5349
Size: 5473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2458 4587 4771 1305
Size: 1153 2129 184 2007
Fragments arranged by size:
2129 2007 1153 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3651
Fragments arranged by size:
3651 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 3431 3790 3949 518
Size: 648 1362 903 359 159 2042
Fragments arranged by size:
2042 1362 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 2889 3566 4714 4959 1606
Size: 436 847 677 1148 245 2120
Fragments arranged by size:
2120 1148 847 677 436 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4921
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5473
NotI GC'GGCC_GC
Cuts at: 2920 2920
Size: 5473
NspI r_CATG'y
Cuts at: 1132 1229 2831 4376 1132
Size: 97 1602 1545 2229
Fragments arranged by size:
2229 1602 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5473
PciI A'CATG_T
Cuts at: 4372 4372
Size: 5473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5473
PmeI GTTT'AAAC
Cuts at: 2777 2777
Size: 5473
PmlI CAC'GTG
Cuts at: 2671 2671
Size: 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2148 2180 3542 3574 5055 5087 2148
Size: 32 1362 32 1481 32 2534
Fragments arranged by size:
2534 1481 1362 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2100 2228 2100
Size: 128 5345
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5473
PsiI TTA'TAA
Cuts at: 3043 3043
Size: 5473
PstI C_TGCA'G
Cuts at: 830 2825 830
Size: 1995 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5473
SalI G'TCGA_C
Cuts at: 1936 2815 1936
Size: 879 4594
SbfI CC_TGCA'GG
Cuts at: 2825 2825
Size: 5473
ScaI AGT'ACT
Cuts at: 2319 3721 5433 2319
Size: 1402 1712 2359
Fragments arranged by size:
2359 1712 1402
SfcI C'TryA_G
Cuts at: 826 1045 2821 3956 4637 4828 826
Size: 219 1776 1135 681 191 1471
Fragments arranged by size:
1776 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5473
SmaI CCC'GGG
Cuts at: 1872 2015 2800 1872
Size: 143 785 4545
Fragments arranged by size:
4545 785 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2754 3577 4478 4740 820
Size: 197 926 811 823 901 262 1553
Fragments arranged by size:
1553 926 901 823 811 262 197
SnaBI TAC'GTA
Cuts at: 493 3264 493
Size: 2771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5473
SphI G_CATG'C
Cuts at: 1132 2831 1132
Size: 1699 3774
SspI AAT'ATT
Cuts at: 5 52 3397 5
Size: 47 3345 2081
Fragments arranged by size:
3345 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5473
StyI C'CwwG_G
Cuts at: 513 1065 2071 2912 513
Size: 552 1006 841 3074
Fragments arranged by size:
3074 1006 841 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 3500 3517 3670 3855 4300 325
Size: 1255 1067 853 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1067 853 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2236 2317 3719
Size: 274 80 33 51 1443 259 81 1402
Cuts at: 3719 5431 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1402 274 259 138 81 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2112 2144 5433 5465 2112
Size: 32 3289 32 2120
Fragments arranged by size:
3289 2120 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5473
XbaI T'CTAG_A
Cuts at: 2809 2809
Size: 5473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5473
XmaI C'CCGG_G
Cuts at: 1870 2013 2798 1870
Size: 143 785 4545
Fragments arranged by size:
4545 785 143
XmnI GAAnn'nnTTC
Cuts at: 3602 3602
Size: 5473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3281 276
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI