Restriction map for
pFN21AE6279
(Circular) (Six-base) MAPSORT of: pFN21AE6279.seq Check: 8514 from: 1 to: 5431
pFN21AE6279 5431 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2255 2255
Size: 5431
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 278
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2752 2752
Size: 5431
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 3494 4878 5146 1632
Size: 497 1365 1384 268 1917
Fragments arranged by size:
1917 1384 1365 497 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 4330 5307 1203
Size: 3127 977 1327
Fragments arranged by size:
3127 1327 977
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 2213 1166
Size: 1047 4384
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 3174 4746 3174
Size: 1572 3859
ApaI G_GGCC'C
Cuts at: 2488 2488
Size: 5431
ApaLI G'TGCA_C
Cuts at: 3488 4644 3488
Size: 1156 4275
ApoI r'AATT_y
Cuts at: 1493 2740 2966 3118 5227 1493
Size: 1247 226 152 2109 1697
Fragments arranged by size:
2109 1697 1247 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2437 2508 2756 1870
Size: 73 99 395 71 248 4545
Fragments arranged by size:
4545 395 248 99 73 71
BamHI G'GATC_C
Cuts at: 2761 3128 2761
Size: 367 5064
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2103 2752 4207 618
Size: 325 217 627 251 65 649 1455 1842
Fragments arranged by size:
1842 1455 649 627 325 251 217 65
BanII G_rGCy'C
Cuts at: 1391 2488 2566 2750 1391
Size: 1097 78 184 4072
Fragments arranged by size:
4072 1097 184 78
BbeI G_GCGC'C
Cuts at: 2042 2107 2042
Size: 65 5366
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2527 928
Size: 971 628 3832
Fragments arranged by size:
3832 971 628
BbvCI CC'TCA_GC
Cuts at: 2159 2159
Size: 5431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3622 3656 1903
Size: 34 1685 34 3678
Fragments arranged by size:
3678 1685 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4533 3323
Size: 1210 4221
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 2510 4042
Size: 107 122 71 631 605 421 417 1532
Cuts at: 4042 136
Size: 1525
Fragments arranged by size:
1532 1525 631 605 421 417 122 107
71
BglII A'GATC_T
Cuts at: 5426 5426
Size: 5431
BlpI GC'TnA_GC
Cuts at: 2848 2848
Size: 5431
Bme1580I G_kGCm'C
Cuts at: 1790 2358 2488 3492 4648 1790
Size: 568 130 1004 1156 2573
Fragments arranged by size:
2573 1156 1004 568 130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2643 3162 4120
Size: 416 53 83 399 24 1226 519 958
Cuts at: 4120 442
Size: 1753
Fragments arranged by size:
1753 1226 958 519 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 4091 1239
Size: 1133 1719 2579
Fragments arranged by size:
2579 1719 1133
Bpu10I CC'TnA_GC
Cuts at: 2159 2159
Size: 5431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 3556 4421 4719 2327
Size: 1229 865 298 3039
Fragments arranged by size:
3039 1229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2246 2542 4094 882
Size: 617 747 296 1552 2219
Fragments arranged by size:
2219 1552 747 617 296
BsaAI yAC'GTr
Cuts at: 493 1204 3222 493
Size: 711 2018 2702
Fragments arranged by size:
2702 2018 711
BsaBI GATnn'nnATC
Cuts at: 5425 5425
Size: 5431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2039 2104 3238 3620
Size: 53 83 186 936 506 65 1134 382
Cuts at: 3620 4255 275
Size: 635 1451
Fragments arranged by size:
1451 1134 936 635 506 382 186 83
65 53
BsaWI w'CCGG_w
Cuts at: 3864 4262 4536 4683 5094 3864
Size: 398 274 147 411 4201
Fragments arranged by size:
4201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5206
Fragments arranged by size:
5206 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2683 1186
Size: 1497 3934
BseYI C'CCAG_C
Cuts at: 4634 5130 4634
Size: 496 4935
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 2464 1163
Size: 573 609 119 4130
Fragments arranged by size:
4130 609 573 119
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2881 3642 4670 1540
Size: 275 186 880 761 1028 2301
Fragments arranged by size:
2301 1028 880 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2235 3492 3577 4648 2235
Size: 1257 85 1071 3018
Fragments arranged by size:
3018 1257 1071 85
BsmI GAATG_Cn'
Cuts at: 2149 2942 3035 2149
Size: 793 93 4545
Fragments arranged by size:
4545 793 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2358 2488 2566 2750 3492 3577
Size: 399 445 123 130 78 184 742 85
Cuts at: 3577 4648 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 445 399 184 130 123
85 78
BspEI T'CCGG_A
Cuts at: 5094 5094
Size: 5431
BspHI T'CATG_A
Cuts at: 3320 5050 3320
Size: 1730 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2255 2786 844
Size: 1411 531 3489
Fragments arranged by size:
3489 1411 531
BsrBI CCG'CTC
Cuts at: 1394 1823 3318 1394
Size: 429 1495 3507
Fragments arranged by size:
3507 1495 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2614 3926 4100 65
Size: 1183 1366 1312 174 1396
Fragments arranged by size:
1396 1366 1312 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4075 4262 1700
Size: 110 2265 187 2869
Fragments arranged by size:
2869 2265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5431
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5431
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5431
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2215
Size: 5431
BstYI r'GATC_y
Cuts at: 1972 2761 3128 3513 3530 4982 5068 5080 5426
Size: 789 367 385 17 1452 86 12 346
Cuts at: 5426 1972
Size: 1977
Fragments arranged by size:
1977 1452 789 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2061 2543 513
Size: 552 939 30 27 482 3401
Fragments arranged by size:
3401 939 552 482 30 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4692
Fragments arranged by size:
4692 683 56
BtsI GCAGTG_nn'
Cuts at: 2208 2358 2947 3741 3761 2208
Size: 150 589 794 20 3878
Fragments arranged by size:
3878 794 589 150 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2878 3767 5396
Size: 54 1425 50 275 18 1048 889 1629
Cuts at: 5396 8
Size: 43
Fragments arranged by size:
1629 1425 1048 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2878 1537
Size: 275 1066 4090
Fragments arranged by size:
4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 2306 3361 1353
Size: 914 39 1055 3423
Fragments arranged by size:
3423 1055 914 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2240 2465 4018 4392 4538 387
Size: 1064 138 651 225 1553 374 146 1280
Fragments arranged by size:
1553 1280 1064 651 374 225 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2372 3494 4091 4878 5146 1239
Size: 393 497 243 1122 597 787 268 1524
Fragments arranged by size:
1524 1122 787 597 497 393 268 243
EcoNI CCTnn'n_nnAGG
Cuts at: 2309 2309
Size: 5431
EcoO109I rG'GnC_Cy
Cuts at: 2485 2662 2684 2485
Size: 177 22 5232
Fragments arranged by size:
5232 177 22
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 2042 2107 4578 1678
Size: 364 65 2471 2531
Fragments arranged by size:
2531 2471 364 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2539 2571 4072 4104 4146
Size: 32 135 32 967 32 1501 32 42
Cuts at: 4146 4178 1373
Size: 32 2626
Fragments arranged by size:
2626 1501 967 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2775 3021 669
Size: 988 281 837 246 3079
Fragments arranged by size:
3079 988 837 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KasI G'GCGC_C
Cuts at: 2038 2103 2038
Size: 65 5366
KpnI G_GTAC'C
Cuts at: 2756 2756
Size: 5431
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4545 4729 1305
Size: 3240 184 2007
Fragments arranged by size:
3240 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3609
Fragments arranged by size:
3609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 3389 3748 3907 518
Size: 648 1047 1176 359 159 2042
Fragments arranged by size:
2042 1176 1047 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2036 2063 2417 2531 2847 3524 4672
Size: 406 24 27 354 114 316 677 1148
Cuts at: 4672 4917 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 406 354 316 245 114
27 24
NarI GG'CG_CC
Cuts at: 2039 2104 2039
Size: 65 5366
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3940
Fragments arranged by size:
3940 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2878 2878
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2789 4334 1132
Size: 97 1560 1545 2229
Fragments arranged by size:
2229 1560 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 4330 4330
Size: 5431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5431
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5013 5045 3500
Size: 32 1481 32 3886
Fragments arranged by size:
3886 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2662 2662
Size: 5431
PshAI GACnn'nnGTC
Cuts at: 1613 2667 1613
Size: 1054 4377
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PspOMI G'GGCC_C
Cuts at: 2484 2484
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2661 2783 830
Size: 1831 122 3478
Fragments arranged by size:
3478 1831 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
PvuII CAG'CTG
Cuts at: 2417 2531 2417
Size: 114 5317
SacII CC_GC'GG
Cuts at: 2037 2064 2037
Size: 27 5404
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SapI GCTCTTCn'nnn_
Cuts at: 2267 2267
Size: 5431
SbfI CC_TGCA'GG
Cuts at: 2783 2783
Size: 5431
ScaI AGT'ACT
Cuts at: 3679 5391 3679
Size: 1712 3719
SexAI A'CCwGG_T
Cuts at: 2292 2292
Size: 5431
SfcI C'TryA_G
Cuts at: 826 1045 2657 2779 3914 4595 4786 826
Size: 219 1612 122 1135 681 191 1471
Fragments arranged by size:
1612 1471 1135 681 219 191 122
SfoI GGC'GCC
Cuts at: 2040 2105 2040
Size: 65 5366
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2044 2510 2758 1872
Size: 172 466 248 4545
Fragments arranged by size:
4545 466 248 172
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 3535 4436 4698 820
Size: 197 926 399 1193 901 262 1553
Fragments arranged by size:
1553 1193 926 901 399 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2789 1132
Size: 1657 3774
SrfI GCCC'GGGC
Cuts at: 2044 2044
Size: 5431
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5431
StyI C'CwwG_G
Cuts at: 513 1065 2004 2870 513
Size: 552 939 866 3074
Fragments arranged by size:
3074 939 866 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3458 3475 3628 3813 4258 325
Size: 1255 1878 17 153 185 445 1498
Fragments arranged by size:
1878 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 3677 5389
Size: 274 80 33 51 1443 135 1565 1712
Cuts at: 5389 96
Size: 138
Fragments arranged by size:
1712 1565 1443 274 138 135 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2074 2106 5391 5423 2074
Size: 32 3285 32 2082
Fragments arranged by size:
3285 2082 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2212
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2042 2508 2756 1870
Size: 172 466 248 4545
Fragments arranged by size:
4545 466 248 172
XmnI GAAnn'nnTTC
Cuts at: 3560 3560
Size: 5431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 276
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SanDI SfiI SwaI