Restriction map for
pFN21AE6267
(Circular) (Six-base) MAPSORT of: pFN21AE6267.seq Check: 1565 from: 1 to: 5608
pFN21AE6267 5608 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2692 2721 3671 5055 5323 1632
Size: 1060 29 950 1384 268 1917
Fragments arranged by size:
1917 1384 1060 950 268 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 2681 1166
Size: 1515 4093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2525 1184
Size: 1341 4267
AlwNI CAG_nnn'CTG
Cuts at: 2077 2329 2414 2497 2836 2893 3351 4923 2077
Size: 252 85 83 339 57 458 1572 2762
Fragments arranged by size:
2762 1572 458 339 252 85 83 57
ApaI G_GGCC'C
Cuts at: 2242 2666 2819 2242
Size: 424 153 5031
Fragments arranged by size:
5031 424 153
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2917 3143 3295 5404 1493
Size: 1424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2606 2652 2933 1870
Size: 73 663 46 281 4545
Fragments arranged by size:
4545 663 281 73 46
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2621 2734 2929 4384 618
Size: 325 217 627 834 113 195 1455 1842
Fragments arranged by size:
1842 1455 834 627 325 217 195 113
BanII G_rGCy'C
Cuts at: 1391 2242 2526 2666 2819 2927 1391
Size: 851 284 140 153 108 4072
Fragments arranged by size:
4072 851 284 153 140 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2002 2698 928
Size: 971 103 696 3838
Fragments arranged by size:
3838 971 696 103
BbvCI CC'TCA_GC
Cuts at: 2881 2881
Size: 5608
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 1903
Size: 34 1862 34 3678
Fragments arranged by size:
3678 1862 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2043 3500 4710 2043
Size: 1457 1210 2941
Fragments arranged by size:
2941 1457 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2242 2504 2666 2819 3669 4825 1790
Size: 452 262 162 153 850 1156 2573
Fragments arranged by size:
2573 1156 850 452 262 162 153
BmgBI CAC'GTC
Cuts at: 1318 2669 1318
Size: 1351 4257
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2514 3339 4297
Size: 416 53 83 399 24 1097 825 958
Cuts at: 4297 442
Size: 1753
Fragments arranged by size:
1753 1097 958 825 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2591 2915 4268 1239
Size: 1352 324 1353 2579
Fragments arranged by size:
2579 1353 1352 324
Bpu10I CC'TnA_GC
Cuts at: 2829 2881 2829
Size: 52 5556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 4598 4896 3733
Size: 865 298 4445
Fragments arranged by size:
4445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2218 2683 3399 493
Size: 711 1014 465 716 2702
Fragments arranged by size:
2702 1014 716 711 465
BsaBI GATnn'nnATC
Cuts at: 2210 2594 5602 2210
Size: 384 3008 2216
Fragments arranged by size:
3008 2216 384
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2284 3415 3797 4432
Size: 53 83 186 936 751 1131 382 635
Cuts at: 4432 275
Size: 1451
Fragments arranged by size:
1451 1131 936 751 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2046 4041 4439 4713 4860 5271 2046
Size: 1995 398 274 147 411 2383
Fragments arranged by size:
2383 1995 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2073 2094 2124 1178
Size: 30 165 30 640 30 21 30 4662
Fragments arranged by size:
4662 640 165 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 1186
Size: 916 4692
BseYI C'CCAG_C
Cuts at: 2691 4811 5307 2691
Size: 2120 496 2992
Fragments arranged by size:
2992 2120 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 2786 1163
Size: 573 606 444 3985
Fragments arranged by size:
3985 606 573 444
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3669 3754 4825 3669
Size: 85 1071 4452
Fragments arranged by size:
4452 1071 85
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
BsmBI CGTCTCn'nnnn_
Cuts at: 2236 2675 2236
Size: 439 5169
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2242 2504 2526 2666 2819 2927 3669
Size: 399 452 262 22 140 153 108 742
Cuts at: 3669 3754 4825 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 399 262 153 140
108 85 22
BspEI T'CCGG_A
Cuts at: 2046 5271 2046
Size: 3225 2383
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2963 844
Size: 2119 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4103 4277 65
Size: 1183 2855 174 1396
Fragments arranged by size:
2855 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 2218 2182
Size: 36 5572
BstYI r'GATC_y
Cuts at: 1972 2833 2938 3305 3690 3707 5159 5245 5257
Size: 861 105 367 385 17 1452 86 12
Cuts at: 5257 5603 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 861 385 367 346 105 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2811 2811
Size: 5608
BtgI C'CryG_G
Cuts at: 513 1065 2004 2616 513
Size: 552 939 612 3505
Fragments arranged by size:
3505 939 612 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 2078 3124 3918 3938 2078
Size: 1046 794 20 3748
Fragments arranged by size:
3748 1046 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2265 2529
Size: 54 1425 50 275 18 366 69 264
Cuts at: 2529 3055 3944 5573 8
Size: 526 889 1629 43
Fragments arranged by size:
1629 1425 889 526 366 275 264 69
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2678 3538 1353
Size: 1325 860 3423
Fragments arranged by size:
3423 1325 860
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2591 2692 2721 2915 3671 4268 5055
Size: 393 959 101 29 194 756 597 787
Cuts at: 5055 5323 1239
Size: 268 1524
Fragments arranged by size:
1524 959 787 756 597 393 268 194
101 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2489 2489
Size: 5608
EcoO109I rG'GnC_Cy
Cuts at: 2020 2238 2656 2696 2815 2020
Size: 218 418 40 119 4813
Fragments arranged by size:
4813 418 218 119 40
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 4755 1678
Size: 3077 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2825 2857 4249 4281 4323
Size: 32 135 32 1253 32 1392 32 42
Cuts at: 4323 4355 1373
Size: 32 2626
Fragments arranged by size:
2626 1392 1253 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2260 2847 4722 4906 1305
Size: 955 587 1875 184 2007
Fragments arranged by size:
2007 1875 955 587 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2267 2531 10
Size: 54 1425 343 435 264 3087
Fragments arranged by size:
3087 1425 435 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2318 2507 2681 3566 3925 4084
Size: 648 1050 102 189 174 885 359 159
Cuts at: 4084 518
Size: 2042
Fragments arranged by size:
2042 1050 885 648 359 189 174 159
102
MspA1I CmG'CkG
Cuts at: 1606 2788 3024 3701 4849 5094 1606
Size: 1182 236 677 1148 245 2120
Fragments arranged by size:
2120 1182 1148 677 245 236
NcoI C'CATG_G
Cuts at: 513 1065 2004 2616 513
Size: 552 939 612 3505
Fragments arranged by size:
3505 939 612 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2292 1348
Size: 944 4664
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2791 1108
Size: 1683 3925
PfoI T'CCnGG_A
Cuts at: 2484 2535 2484
Size: 51 5557
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2656 2696 2656
Size: 40 5568
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PspOMI G'GGCC_C
Cuts at: 2238 2662 2815 2238
Size: 424 153 5031
Fragments arranged by size:
5031 424 153
PstI C_TGCA'G
Cuts at: 830 2960 830
Size: 2130 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2788 2788
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2381 2956 4091 4772 4963 826
Size: 219 1336 575 1135 681 191 1471
Fragments arranged by size:
1471 1336 1135 681 575 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2654 2935 1872
Size: 782 281 4545
Fragments arranged by size:
4545 782 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3712 4613 4875 820
Size: 197 926 1769 901 262 1553
Fragments arranged by size:
1769 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2004 2119 2421 2616 3047 513
Size: 552 939 115 302 195 431 3074
Fragments arranged by size:
3074 939 552 431 302 195 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2081 2132 2210 2609 2722 3635 3652
Size: 1255 501 51 78 399 113 913 17
Cuts at: 3652 3805 3990 4435 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 913 501 445 399 185 153
113 78 51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2101 3854 5566
Size: 274 80 33 51 1443 124 1753 1712
Cuts at: 5566 96
Size: 138
Fragments arranged by size:
1753 1712 1443 274 138 124 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2314 2314
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2652 2933 1870
Size: 782 281 4545
Fragments arranged by size:
4545 782 281
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI