Restriction map for
pFN21AE6262
(Circular) (Six-base) MAPSORT of: pFN21AE6262.seq Check: 743 from: 1 to: 5539
pFN21AE6262 5539 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2882 1937
Size: 945 4594
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2690 2823 3602 4986 5254 1632
Size: 1058 133 779 1384 268 1917
Fragments arranged by size:
1917 1384 1058 779 268 133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5539
AlwNI CAG_nnn'CTG
Cuts at: 3282 4854 3282
Size: 1572 3967
ApaI G_GGCC'C
Cuts at: 2110 2110
Size: 5539
ApaLI G'TGCA_C
Cuts at: 3596 4752 3596
Size: 1156 4383
ApoI r'AATT_y
Cuts at: 1493 2750 2785 2848 3074 3226 5335 1493
Size: 1257 35 63 226 152 2109 1697
Fragments arranged by size:
2109 1697 1257 226 152 63 35
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 2178 2864 1870
Size: 73 166 69 686 4545
Fragments arranged by size:
4545 686 166 73 69
BamHI G'GATC_C
Cuts at: 2326 2869 3236 2326
Size: 543 367 4629
Fragments arranged by size:
4629 543 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2100 2860 4315 618
Size: 325 217 627 313 760 1455 1842
Fragments arranged by size:
1842 1455 760 627 325 313 217
BanII G_rGCy'C
Cuts at: 1391 2110 2858 1391
Size: 719 748 4072
Fragments arranged by size:
4072 748 719
BbeI G_GCGC'C
Cuts at: 2104 2104
Size: 5539
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 1903
Size: 34 1793 34 3678
Fragments arranged by size:
3678 1793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 2631 1548
Size: 1083 4456
BfrBI ATG'CAT
Cuts at: 2677 2677
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2260 5534 2260
Size: 3274 2265
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 2110 3600 4756 1790
Size: 320 1490 1156 2573
Fragments arranged by size:
2573 1490 1156 320
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5539
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2377 3270 4228
Size: 416 53 83 399 24 960 893 958
Cuts at: 4228 442
Size: 1753
Fragments arranged by size:
1753 960 958 893 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4199 1239
Size: 2960 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3664 4529 4827 3664
Size: 865 298 4376
Fragments arranged by size:
4376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3330 493
Size: 711 2126 2702
Fragments arranged by size:
2702 2126 711
BsaBI GATnn'nnATC
Cuts at: 2187 5533 2187
Size: 3346 2193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2101 2121 3346 3728
Size: 53 83 186 936 568 20 1225 382
Cuts at: 3728 4363 275
Size: 635 1451
Fragments arranged by size:
1451 1225 936 635 568 382 186 83
53 20
BsaWI w'CCGG_w
Cuts at: 2056 3972 4370 4644 4791 5202 2056
Size: 1916 398 274 147 411 2393
Fragments arranged by size:
2393 1916 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5539
BseYI C'CCAG_C
Cuts at: 2022 4742 5238 2022
Size: 2720 496 2323
Fragments arranged by size:
2720 2323 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2277 1163
Size: 573 541 4425
Fragments arranged by size:
4425 573 541
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2989 3750 4778 1540
Size: 275 186 988 761 1028 2301
Fragments arranged by size:
2301 1028 988 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3600 3685 4756 3600
Size: 85 1071 4383
Fragments arranged by size:
4383 1071 85
BsmI GAATG_Cn'
Cuts at: 2832 3050 3143 2832
Size: 218 93 5228
Fragments arranged by size:
5228 218 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2858 3600 3685 4756 1391
Size: 399 320 748 742 85 1071 2174
Fragments arranged by size:
2174 1071 748 742 399 320 85
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2894 844
Size: 2050 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2128 2143 3426 1394
Size: 429 305 15 1283 3507
Fragments arranged by size:
3507 1283 429 305 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4183 4370 1700
Size: 110 2373 187 2869
Fragments arranged by size:
2869 2373 187 110
BsrGI T'GTAC_A
Cuts at: 96 2434 96
Size: 2338 3201
BssHII G'CGCG_C
Cuts at: 1925 2173 1925
Size: 248 5291
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5539
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2260 2318 2326 2869 3236 3621 3638 5090
Size: 288 58 8 543 367 385 17 1452
Cuts at: 5090 5176 5188 5534 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 543 385 367 346 288 86
58 17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2004 2305 2757 513
Size: 552 939 301 452 3295
Fragments arranged by size:
3295 939 552 452 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 3055 3849 3869 3055
Size: 794 20 4725
Fragments arranged by size:
4725 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2378 4546 809
Size: 507 1062 2168 1802
Fragments arranged by size:
2168 1802 1062 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504
Size: 54 1425 50 275 18 1156 889 1629
Cuts at: 5504 8
Size: 43
Fragments arranged by size:
1629 1425 1156 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2986 1537
Size: 275 1174 4090
Fragments arranged by size:
4090 1174 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3469 1353
Size: 2116 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4126 4500 4646 387
Size: 1064 138 2537 374 146 1280
Fragments arranged by size:
2537 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2690 2823 3602 4199 4986 5254 1239
Size: 393 1058 133 779 597 787 268 1524
Fragments arranged by size:
1524 1058 787 779 597 393 268 133
EcoNI CCTnn'n_nnAGG
Cuts at: 2291 2291
Size: 5539
EcoO109I rG'GnC_Cy
Cuts at: 2371 2371
Size: 5539
EcoRI G'AATT_C
Cuts at: 2750 2848 3226 2750
Size: 98 378 5063
Fragments arranged by size:
5063 378 98
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 5539
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2096 2104 4686 1678
Size: 418 8 2582 2531
Fragments arranged by size:
2582 2531 418 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 4180 4212 4254
Size: 32 135 32 431 32 2145 32 42
Cuts at: 4254 4286 1373
Size: 32 2626
Fragments arranged by size:
2626 2145 431 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3129 669
Size: 988 281 945 246 3079
Fragments arranged by size:
3079 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 2698 748
Size: 1950 3589
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KasI G'GCGC_C
Cuts at: 2100 2100
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4653 4837 1305
Size: 3348 184 2007
Fragments arranged by size:
3348 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2676 3497 3856 4015 518
Size: 648 1510 821 359 159 2042
Fragments arranged by size:
2042 1510 821 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2455 2955 3632 4780 5025 1606
Size: 531 318 500 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 500 318 245
NarI GG'CG_CC
Cuts at: 2101 2101
Size: 5539
NcoI C'CATG_G
Cuts at: 513 1065 2004 2305 2757 513
Size: 552 939 301 452 3295
Fragments arranged by size:
3295 939 552 452 301
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NsiI A_TGCA'T
Cuts at: 2679 2679
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2677 2897 4442 1132
Size: 97 1448 220 1545 2229
Fragments arranged by size:
2229 1545 1448 220 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5539
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 2371
Size: 5539
PshAI GACnn'nnGTC
Cuts at: 1613 2769 1613
Size: 1156 4383
PsiI TTA'TAA
Cuts at: 2226 2792 3109 2226
Size: 566 317 4656
Fragments arranged by size:
4656 566 317
PspOMI G'GGCC_C
Cuts at: 2106 2106
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2079 2891 830
Size: 1249 812 3478
Fragments arranged by size:
3478 1249 812
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SanDI GG'GwC_CC
Cuts at: 2371 2371
Size: 5539
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SfcI C'TryA_G
Cuts at: 826 1045 2075 2887 4022 4703 4894 826
Size: 219 1030 812 1135 681 191 1471
Fragments arranged by size:
1471 1135 1030 812 681 219 191
SfoI GGC'GCC
Cuts at: 2102 2102
Size: 5539
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2111 2180 2866 1872
Size: 239 69 686 4545
Fragments arranged by size:
4545 686 239 69
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3643 4544 4806 820
Size: 197 926 1700 901 262 1553
Fragments arranged by size:
1700 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5539
StyI C'CwwG_G
Cuts at: 513 1065 2004 2305 2757 2978 513
Size: 552 939 301 452 221 3074
Fragments arranged by size:
3074 939 552 452 301 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3566 3583 3736 3921 4366 325
Size: 1255 1986 17 153 185 445 1498
Fragments arranged by size:
1986 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2434 2548 3785
Size: 274 80 33 51 1443 457 114 1237
Cuts at: 3785 5497 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1237 457 274 138 114 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5539
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2109 2178 2864 1870
Size: 239 69 686 4545
Fragments arranged by size:
4545 686 239 69
XmnI GAAnn'nnTTC
Cuts at: 2667 3668 2667
Size: 1001 4538
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI XcmI