Restriction map for
pFN21AE6259
(Circular) (Six-base) MAPSORT of: pFN21AE6259.seq Check: 9409 from: 1 to: 5428
pFN21AE6259 5428 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2596 2771 1937
Size: 659 175 4594
Fragments arranged by size:
4594 659 175
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2636 2703 3491 4875 5143 1632
Size: 1004 67 788 1384 268 1917
Fragments arranged by size:
1917 1384 1004 788 268 67
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 4327 5304 1203
Size: 3124 977 1327
Fragments arranged by size:
3124 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 2558 3171 4743 2558
Size: 613 1572 3243
Fragments arranged by size:
3243 1572 613
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2737 2963 3115 5224 1493
Size: 1244 226 152 2109 1697
Fragments arranged by size:
2109 1697 1244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 1870
Size: 73 810 4545
Fragments arranged by size:
4545 810 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2202 2749 4204 618
Size: 325 217 627 415 547 1455 1842
Fragments arranged by size:
1842 1455 627 547 415 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 2165 2534 2747 1391
Size: 631 143 369 213 4072
Fragments arranged by size:
4072 631 369 213 143
BbeI G_GCGC'C
Cuts at: 2206 2206
Size: 5428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2666 2715 928
Size: 971 767 49 3641
Fragments arranged by size:
3641 971 767 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3619 3653 1903
Size: 34 1682 34 3678
Fragments arranged by size:
3678 1682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 2520 2649 1548
Size: 972 129 4327
Fragments arranged by size:
4327 972 129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4039 136
Size: 107 122 71 631 605 2367 1525
Fragments arranged by size:
2367 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2845 2845
Size: 5428
Bme1580I G_kGCm'C
Cuts at: 1790 3489 4645 1790
Size: 1699 1156 2573
Fragments arranged by size:
2573 1699 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2537 3159 4117
Size: 416 53 83 399 24 1120 622 958
Cuts at: 4117 442
Size: 1753
Fragments arranged by size:
1753 1120 958 622 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4088 1239
Size: 2849 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 2414 3553 4418 4716 2135
Size: 279 1139 865 298 2847
Fragments arranged by size:
2847 1139 865 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2039 4091 882
Size: 617 540 2052 2219
Fragments arranged by size:
2219 2052 617 540
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2203 3235 3617
Size: 53 83 186 936 533 137 1032 382
Cuts at: 3617 4252 275
Size: 635 1451
Fragments arranged by size:
1451 1032 936 635 533 382 186 137
83 53
BsaWI w'CCGG_w
Cuts at: 3861 4259 4533 4680 5091 3861
Size: 398 274 147 411 4198
Fragments arranged by size:
4198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 2105 2135 2589
Size: 30 165 30 648 30 24 30 454
Cuts at: 2589 2619 1178
Size: 30 3987
Fragments arranged by size:
3987 648 454 165 30 30 30 30
30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2327 1186
Size: 826 315 4287
Fragments arranged by size:
4287 826 315
BseYI C'CCAG_C
Cuts at: 2158 2551 4631 5127 2158
Size: 393 2080 496 2459
Fragments arranged by size:
2459 2080 496 393
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 1163
Size: 573 387 4468
Fragments arranged by size:
4468 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 2878 3639 4667 1540
Size: 275 186 37 840 761 1028 2301
Fragments arranged by size:
2301 1028 840 761 275 186 37
BsiHKAI G_wGCw'C
Cuts at: 2514 3489 3574 4645 2514
Size: 975 85 1071 3297
Fragments arranged by size:
3297 1071 975 85
BsmI GAATG_Cn'
Cuts at: 2939 3032 2939
Size: 93 5335
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2165 2514 2534 2747 3489 3574
Size: 399 232 143 349 20 213 742 85
Cuts at: 3574 4645 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 349 232 213 143
85 20
BspEI T'CCGG_A
Cuts at: 5091 5091
Size: 5428
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2783 844
Size: 1295 644 3489
Fragments arranged by size:
3489 1295 644
BsrBI CCG'CTC
Cuts at: 1394 1823 3315 1394
Size: 429 1492 3507
Fragments arranged by size:
3507 1492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3923 4097 65
Size: 1183 2675 174 1396
Fragments arranged by size:
2675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2643 4072 4259 1700
Size: 110 833 1429 187 2869
Fragments arranged by size:
2869 1429 833 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 3488 4500 3488
Size: 1012 4416
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2101 794
Size: 1307 4121
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstXI CCAn_nnnn'nTGG
Cuts at: 2348 2348
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2758 3125 3510 3527 4979 5065 5077 5423
Size: 786 367 385 17 1452 86 12 346
Cuts at: 5423 1972
Size: 1977
Fragments arranged by size:
1977 1452 786 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4876
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 2944 3738 3758 2069
Size: 875 794 20 3739
Fragments arranged by size:
3739 875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DraIII CAC_nnn'GTG
Cuts at: 2518 2518
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4435 809
Size: 507 3119 1802
Fragments arranged by size:
3119 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2875 3764
Size: 54 1425 50 275 18 205 840 889
Cuts at: 3764 5393 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 840 275 205 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 2875 1537
Size: 275 223 840 4090
Fragments arranged by size:
4090 840 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 3358 1353
Size: 2005 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2113 2528 4015 4389 4535 387
Size: 1064 138 524 415 1487 374 146 1280
Fragments arranged by size:
1487 1280 1064 524 415 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2636 2703 3491 4088 4875 5143 1239
Size: 393 1004 67 788 597 787 268 1524
Fragments arranged by size:
1524 1004 788 787 597 393 268 67
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 2027
Size: 5428
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 2086 2206 4575 1678
Size: 408 120 2369 2531
Fragments arranged by size:
2531 2369 408 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2412 2444 4069
Size: 32 135 32 479 32 329 32 1625
Cuts at: 4069 4101 4143 4175 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1625 479 329 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 2772 3018 669
Size: 988 281 436 398 246 3079
Fragments arranged by size:
3079 988 436 398 281 246
HindIII A'AGCT_T
Cuts at: 748 2468 748
Size: 1720 3708
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KasI G'GCGC_C
Cuts at: 2202 2202
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4542 4726 1305
Size: 3237 184 2007
Fragments arranged by size:
3237 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3606
Fragments arranged by size:
3606 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2454 3386 3745 3904 518
Size: 648 1288 932 359 159 2042
Fragments arranged by size:
2042 1288 932 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2558 2844 3521 4669 4914 1606
Size: 952 286 677 1148 245 2120
Fragments arranged by size:
2120 1148 952 677 286 245
NarI GG'CG_CC
Cuts at: 2203 2203
Size: 5428
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4876
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2035 2875 2035
Size: 840 4588
NspI r_CATG'y
Cuts at: 1132 1229 2056 2179 2441 2786 4331 1132
Size: 97 827 123 262 345 1545 2229
Fragments arranged by size:
2229 1545 827 345 262 123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2199 2780 830
Size: 1369 581 3478
Fragments arranged by size:
3478 1369 581
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
PvuII CAG'CTG
Cuts at: 2558 2558
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SfcI C'TryA_G
Cuts at: 826 1045 2195 2597 2776 3911 4592 4783 826
Size: 219 1150 402 179 1135 681 191 1471
Fragments arranged by size:
1471 1150 1135 681 402 219 191 179
SfoI GGC'GCC
Cuts at: 2204 2204
Size: 5428
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 2429 3532 4433 4695 820
Size: 197 926 207 279 1103 901 262 1553
Fragments arranged by size:
1553 1103 926 901 279 262 207 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 2191 3352 5
Size: 47 2139 1161 2081
Fragments arranged by size:
2139 2081 1161 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2169 2252 2367 2590 2867 513
Size: 552 1104 83 115 223 277 3074
Fragments arranged by size:
3074 1104 552 277 223 115 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 3455 3472 3625 3810 4255 325
Size: 1255 483 1392 17 153 185 445 1498
Fragments arranged by size:
1498 1392 1255 483 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3674 5386 96
Size: 274 80 33 51 1443 1697 1712 138
Fragments arranged by size:
1712 1697 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2105 2137 2343 2375 5388 5420 2105
Size: 32 206 32 3013 32 2113
Fragments arranged by size:
3013 2113 206 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI