Restriction map for
pFN21AE6258
(Circular) (Six-base) MAPSORT of: pFN21AE6258.seq Check: 7115 from: 1 to: 5455
pFN21AE6258 5455 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3265 278
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2700 2798 1937
Size: 763 98 4594
Fragments arranged by size:
4594 763 98
Acc65I G'GTAC_C
Cuts at: 2776 2776
Size: 5455
AclI AA'CG_TT
Cuts at: 1563 3582 3955 1563
Size: 2019 373 3063
Fragments arranged by size:
3063 2019 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3518 4902 5170 1632
Size: 1886 1384 268 1917
Fragments arranged by size:
1917 1886 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 4354 5331 1203
Size: 3151 977 1327
Fragments arranged by size:
3151 1327 977
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 2561 1166
Size: 1395 4060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2206 1184
Size: 1022 4433
AlwNI CAG_nnn'CTG
Cuts at: 2273 3198 4770 2273
Size: 925 1572 2958
Fragments arranged by size:
2958 1572 925
ApaI G_GGCC'C
Cuts at: 2621 2621
Size: 5455
ApaLI G'TGCA_C
Cuts at: 3512 4668 3512
Size: 1156 4299
ApoI r'AATT_y
Cuts at: 1493 2764 2990 3142 5251 1493
Size: 1271 226 152 2109 1697
Fragments arranged by size:
2109 1697 1271 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2780 1870
Size: 73 837 4545
Fragments arranged by size:
4545 837 73
BamHI G'GATC_C
Cuts at: 2668 2730 2785 3152 2668
Size: 62 55 367 4971
Fragments arranged by size:
4971 367 62 55
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2420 2776 4231 618
Size: 325 217 627 633 356 1455 1842
Fragments arranged by size:
1842 1455 633 627 356 325 217
BanII G_rGCy'C
Cuts at: 1391 2621 2774 1391
Size: 1230 153 4072
Fragments arranged by size:
4072 1230 153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2135 928
Size: 971 236 4248
Fragments arranged by size:
4248 971 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3646 3680 1903
Size: 34 1709 34 3678
Fragments arranged by size:
3678 1709 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4557 3347
Size: 1210 4245
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4066 136
Size: 107 122 71 631 605 2394 1525
Fragments arranged by size:
2394 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5450 5450
Size: 5455
BlpI GC'TnA_GC
Cuts at: 2872 2872
Size: 5455
Bme1580I G_kGCm'C
Cuts at: 1790 2621 3516 4672 1790
Size: 831 895 1156 2573
Fragments arranged by size:
2573 1156 895 831
BmgBI CAC'GTC
Cuts at: 1318 2454 1318
Size: 1136 4319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 3186 4144
Size: 416 53 83 399 24 1041 728 958
Cuts at: 4144 442
Size: 1753
Fragments arranged by size:
1753 1041 958 728 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 4115 1239
Size: 980 1896 2579
Fragments arranged by size:
2579 1896 980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 3580 4445 4743 2180
Size: 1400 865 298 2892
Fragments arranged by size:
2892 1400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 4118 882
Size: 617 555 2064 2219
Fragments arranged by size:
2219 2064 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 5449 5449
Size: 5455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3262 3644 4279 275
Size: 53 83 186 936 1729 382 635 1451
Fragments arranged by size:
1729 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3888 4286 4560 4707 5118 3888
Size: 398 274 147 411 4225
Fragments arranged by size:
4225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5230
Fragments arranged by size:
5230 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2389 1186
Size: 1072 131 4252
Fragments arranged by size:
4252 1072 131
BseYI C'CCAG_C
Cuts at: 2100 2442 4658 5154 2100
Size: 342 2216 496 2401
Fragments arranged by size:
2401 2216 496 342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2597 1163
Size: 573 861 4021
Fragments arranged by size:
4021 861 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3666 4694 1540
Size: 275 186 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 3516 3601 4672 2517
Size: 999 85 1071 3300
Fragments arranged by size:
3300 1071 999 85
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
BsmBI CGTCTCn'nnnn_
Cuts at: 2732 2732
Size: 5455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2517 2621 2774 3516 3601 4672 1391
Size: 399 727 104 153 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 727 399 153 104 85
BspEI T'CCGG_A
Cuts at: 5118 5118
Size: 5455
BspHI T'CATG_A
Cuts at: 3344 5074 3344
Size: 1730 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2810 844
Size: 1966 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3342 1394
Size: 429 1519 3507
Fragments arranged by size:
3507 1519 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2335 3950 4124 65
Size: 1183 1087 1615 174 1396
Fragments arranged by size:
1615 1396 1183 1087 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4099 4286 1700
Size: 110 2289 187 2869
Fragments arranged by size:
2869 2289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5455
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5455
BssSI C'ACGA_G
Cuts at: 3515 4527 3515
Size: 1012 4443
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2427 794
Size: 1633 3822
BstBI TT'CG_AA
Cuts at: 4259 4259
Size: 5455
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5455
BstYI r'GATC_y
Cuts at: 1972 2668 2730 2785 3152 3537 3554 5006 5092
Size: 696 62 55 367 385 17 1452 86
Cuts at: 5092 5104 5450 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 696 385 367 346 86 62
55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2559 513
Size: 552 1494 3409
Fragments arranged by size:
3409 1494 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2143 2340 2503 487
Size: 683 56 917 197 163 3439
Fragments arranged by size:
3439 917 683 197 163 56
BtsI GCAGTG_nn'
Cuts at: 2188 2412 2971 3765 3785 2188
Size: 224 559 794 20 3858
Fragments arranged by size:
3858 794 559 224 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5420
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DraIII CAC_nnn'GTG
Cuts at: 2408 2408
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2902 3791 5420
Size: 54 1425 50 275 18 1072 889 1629
Cuts at: 5420 8
Size: 43
Fragments arranged by size:
1629 1425 1072 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2902 1537
Size: 275 1090 4090
Fragments arranged by size:
4090 1090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2646 3385 1353
Size: 1293 739 3423
Fragments arranged by size:
3423 1293 739
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4042 4416 4562 387
Size: 1064 138 2453 374 146 1280
Fragments arranged by size:
2453 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 3518 4115 4902 5170 1239
Size: 393 587 1299 597 787 268 1524
Fragments arranged by size:
1524 1299 787 597 587 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2237 2237
Size: 5455
EcoO109I rG'GnC_Cy
Cuts at: 2617 2618 2617
Size: 1 5454
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FspI TGC'GCA
Cuts at: 3961 3961
Size: 5455
HaeII r_GCGC'y
Cuts at: 1678 4602 1678
Size: 2924 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373
Size: 32 135 32 2524 32 42 32 2626
Fragments arranged by size:
2626 2524 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2799 3045 669
Size: 988 281 861 246 3079
Fragments arranged by size:
3079 988 861 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 3045 3045
Size: 5455
KpnI G_GTAC'C
Cuts at: 2780 2780
Size: 5455
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 5331 5331
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4569 4753 1305
Size: 3264 184 2007
Fragments arranged by size:
3264 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3633
Fragments arranged by size:
3633 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 2342 2561 3413 3772 3931 518
Size: 648 1162 14 219 852 359 159 2042
Fragments arranged by size:
2042 1162 852 648 359 219 159 14
MspA1I CmG'CkG
Cuts at: 1606 2427 2871 3548 4696 4941 1606
Size: 821 444 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 677 444 245
NcoI C'CATG_G
Cuts at: 513 1065 2559 513
Size: 552 1494 3409
Fragments arranged by size:
3409 1494 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5455
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2813 4358 1132
Size: 97 1584 1545 2229
Fragments arranged by size:
2229 1584 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5455
PciI A'CATG_T
Cuts at: 4354 4354
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2373 1108
Size: 1265 4190
PfoI T'CCnGG_A
Cuts at: 2733 2733
Size: 5455
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 5037 5069 3524
Size: 32 1481 32 3910
Fragments arranged by size:
3910 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5455
PsiI TTA'TAA
Cuts at: 3025 3025
Size: 5455
PspOMI G'GGCC_C
Cuts at: 2617 2617
Size: 5455
PstI C_TGCA'G
Cuts at: 830 2019 2807 830
Size: 1189 788 3478
Fragments arranged by size:
3478 1189 788
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
PvuII CAG'CTG
Cuts at: 2427 2427
Size: 5455
SalI G'TCGA_C
Cuts at: 1936 2797 1936
Size: 861 4594
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 3703 5415 3703
Size: 1712 3743
SfcI C'TryA_G
Cuts at: 826 1045 2015 2803 3938 4619 4810 826
Size: 219 970 788 1135 681 191 1471
Fragments arranged by size:
1471 1135 970 788 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5455
SmaI CCC'GGG
Cuts at: 1872 2782 1872
Size: 910 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2195 3559 4460 4722 820
Size: 197 926 252 1364 901 262 1553
Fragments arranged by size:
1553 1364 926 901 262 252 197
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2813 1132
Size: 1681 3774
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5455
StyI C'CwwG_G
Cuts at: 513 1065 2559 2894 513
Size: 552 1494 335 3074
Fragments arranged by size:
3074 1494 552 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3482 3499 3652 3837 4282 325
Size: 1255 1902 17 153 185 445 1498
Fragments arranged by size:
1902 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3701 5413 96
Size: 274 80 33 51 1443 1724 1712 138
Fragments arranged by size:
1724 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5415 5447 5415
Size: 32 5423
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5455
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5455
XmaI C'CCGG_G
Cuts at: 1870 2780 1870
Size: 910 4545
XmnI GAAnn'nnTTC
Cuts at: 3584 3584
Size: 5455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3263 276
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI