Restriction map for
pFN21AE6257
(Circular) (Six-base) MAPSORT of: pFN21AE6257.seq Check: 306 from: 1 to: 5446
pFN21AE6257 5446 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3256 278
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2789 1937
Size: 852 4594
Acc65I G'GTAC_C
Cuts at: 2767 2767
Size: 5446
AclI AA'CG_TT
Cuts at: 1563 3573 3946 1563
Size: 2010 373 3063
Fragments arranged by size:
3063 2010 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2466 3509 4893 5161 1632
Size: 834 1043 1384 268 1917
Fragments arranged by size:
1917 1384 1043 834 268
AflII C'TTAA_G
Cuts at: 820 1017 2285 820
Size: 197 1268 3981
Fragments arranged by size:
3981 1268 197
AflIII A'CryG_T
Cuts at: 1203 4345 5322 1203
Size: 3142 977 1327
Fragments arranged by size:
3142 1327 977
AgeI A'CCGG_T
Cuts at: 2187 4277 2187
Size: 2090 3356
AhdI GACnn_n'nnGTC
Cuts at: 4175 4175
Size: 5446
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5446
AlwNI CAG_nnn'CTG
Cuts at: 2666 3189 4761 2666
Size: 523 1572 3351
Fragments arranged by size:
3351 1572 523
ApaLI G'TGCA_C
Cuts at: 3503 4659 3503
Size: 1156 4290
ApoI r'AATT_y
Cuts at: 1493 2755 2981 3133 5242 1493
Size: 1262 226 152 2109 1697
Fragments arranged by size:
2109 1697 1262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4000 160
Size: 872 2968 1606
Fragments arranged by size:
2968 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 1870
Size: 73 828 4545
Fragments arranged by size:
4545 828 73
BamHI G'GATC_C
Cuts at: 2448 2776 3143 2448
Size: 328 367 4751
Fragments arranged by size:
4751 367 328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2767 4222 618
Size: 325 217 627 980 1455 1842
Fragments arranged by size:
1842 1455 980 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2682 2765 1391
Size: 1291 83 4072
Fragments arranged by size:
4072 1291 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2389 928
Size: 971 490 3985
Fragments arranged by size:
3985 971 490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2020 2054 3637 3671 1903
Size: 34 83 34 1583 34 3678
Fragments arranged by size:
3678 1583 83 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3338 4548 3338
Size: 1210 4236
BclI T'GATC_A
Cuts at: 1548 2070 1548
Size: 522 4924
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4057 136
Size: 107 122 71 631 605 337 2048 1525
Fragments arranged by size:
2048 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5441 5441
Size: 5446
BlpI GC'TnA_GC
Cuts at: 2863 2863
Size: 5446
Bme1580I G_kGCm'C
Cuts at: 1790 3507 4663 1790
Size: 1717 1156 2573
Fragments arranged by size:
2573 1717 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5446
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2575 3177 4135
Size: 416 53 83 399 24 1158 602 958
Cuts at: 4135 442
Size: 1753
Fragments arranged by size:
1753 1158 958 602 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5446
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2726 2726
Size: 5446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2721 4106 1239
Size: 950 532 1385 2579
Fragments arranged by size:
2579 1385 950 532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3571 4436 4734 3571
Size: 865 298 4283
Fragments arranged by size:
4283 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2140 2375 4109 882
Size: 617 641 235 1734 2219
Fragments arranged by size:
2219 1734 641 617 235
BsaAI yAC'GTr
Cuts at: 493 1204 3237 493
Size: 711 2033 2702
Fragments arranged by size:
2702 2033 711
BsaBI GATnn'nnATC
Cuts at: 5440 5440
Size: 5446
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3253 3635 4270 275
Size: 53 83 186 936 1720 382 635 1451
Fragments arranged by size:
1720 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2187 3879 4277 4551 4698 5109 2187
Size: 1692 398 274 147 411 2524
Fragments arranged by size:
2524 1692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5221
Fragments arranged by size:
5221 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2569 1186
Size: 1383 4063
BseYI C'CCAG_C
Cuts at: 2081 4649 5145 2081
Size: 2568 496 2382
Fragments arranged by size:
2568 2382 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2014 1163
Size: 573 278 4595
Fragments arranged by size:
4595 573 278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2188 2896 3657 4685 1540
Size: 275 186 187 708 761 1028 2301
Fragments arranged by size:
2301 1028 761 708 275 187 186
BsiHKAI G_wGCw'C
Cuts at: 3507 3592 4663 3507
Size: 85 1071 4290
Fragments arranged by size:
4290 1071 85
BsmI GAATG_Cn'
Cuts at: 2957 3050 2957
Size: 93 5353
BsmBI CGTCTCn'nnnn_
Cuts at: 2551 2551
Size: 5446
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2682 2765 3507 3592 4663 1391
Size: 399 892 83 742 85 1071 2174
Fragments arranged by size:
2174 1071 892 742 399 85 83
BspEI T'CCGG_A
Cuts at: 5109 5109
Size: 5446
BspHI T'CATG_A
Cuts at: 3335 5065 3335
Size: 1730 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2801 844
Size: 1957 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3333 1394
Size: 429 1510 3507
Fragments arranged by size:
3507 1510 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3941 4115 65
Size: 1183 2693 174 1396
Fragments arranged by size:
2693 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2187 4090 4277 1700
Size: 110 377 1903 187 2869
Fragments arranged by size:
2869 1903 377 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5446
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5446
BssSI C'ACGA_G
Cuts at: 3506 4518 3506
Size: 1012 4434
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5446
BstBI TT'CG_AA
Cuts at: 4250 4250
Size: 5446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5446
BstYI r'GATC_y
Cuts at: 1972 2448 2776 3143 3528 3545 4997 5083 5095
Size: 476 328 367 385 17 1452 86 12
Cuts at: 5095 5441 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 476 385 367 346 328 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2452 2673 513
Size: 552 939 448 221 3286
Fragments arranged by size:
3286 939 552 448 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2295 487
Size: 683 56 1069 3638
Fragments arranged by size:
3638 1069 683 56
BtsI GCAGTG_nn'
Cuts at: 2962 3756 3776 2962
Size: 794 20 4632
Fragments arranged by size:
4632 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5411
DraI TTT'AAA
Cuts at: 2750 2750
Size: 5446
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2188 4453 809
Size: 507 872 2265 1802
Fragments arranged by size:
2265 1802 872 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2893 3782 5411
Size: 54 1425 50 275 18 1063 889 1629
Cuts at: 5411 8
Size: 43
Fragments arranged by size:
1629 1425 1063 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2893 1537
Size: 275 1081 4090
Fragments arranged by size:
4090 1081 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3376 1353
Size: 2023 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2336 4033 4407 4553 387
Size: 1064 138 747 1697 374 146 1280
Fragments arranged by size:
1697 1280 1064 747 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2466 2721 3509 4106 4893 5161
Size: 393 557 277 255 788 597 787 268
Cuts at: 5161 1239
Size: 1524
Fragments arranged by size:
1524 788 787 597 557 393 277 268
255
EcoNI CCTnn'n_nnAGG
Cuts at: 2249 2551 2249
Size: 302 5144
EcoO109I rG'GnC_Cy
Cuts at: 2086 2706 2086
Size: 620 4826
EcoRI G'AATT_C
Cuts at: 2755 3133 2755
Size: 378 5068
FspI TGC'GCA
Cuts at: 3952 3952
Size: 5446
HaeII r_GCGC'y
Cuts at: 1678 4593 1678
Size: 2915 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2322 2347 2354 4087
Size: 32 135 32 743 7 25 7 1733
Cuts at: 4087 4119 4161 4193 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1733 743 135 42 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2790 3036 669
Size: 988 281 852 246 3079
Fragments arranged by size:
3079 988 852 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5446
HpaI GTT'AAC
Cuts at: 3036 3036
Size: 5446
KpnI G_GTAC'C
Cuts at: 2771 2771
Size: 5446
MfeI C'AATT_G
Cuts at: 3045 3045
Size: 5446
MluI A'CGCG_T
Cuts at: 5322 5322
Size: 5446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4560 4744 1305
Size: 3255 184 2007
Fragments arranged by size:
3255 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3624
Fragments arranged by size:
3624 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3404 3763 3922 518
Size: 648 2238 359 159 2042
Fragments arranged by size:
2238 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2699 2862 3539 4687 4932 1606
Size: 1093 163 677 1148 245 2120
Fragments arranged by size:
2120 1148 1093 677 245 163
NcoI C'CATG_G
Cuts at: 513 1065 2004 2452 2673 513
Size: 552 939 448 221 3286
Fragments arranged by size:
3286 939 552 448 221
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5446
NotI GC'GGCC_GC
Cuts at: 2893 2893
Size: 5446
NspI r_CATG'y
Cuts at: 1132 1229 2804 4349 1132
Size: 97 1575 1545 2229
Fragments arranged by size:
2229 1575 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5446
PciI A'CATG_T
Cuts at: 4345 4345
Size: 5446
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5446
PmeI GTTT'AAAC
Cuts at: 2750 2750
Size: 5446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 5028 5060 3515
Size: 32 1481 32 3901
Fragments arranged by size:
3901 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 2706 2086
Size: 620 4826
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5446
PsiI TTA'TAA
Cuts at: 3016 3016
Size: 5446
PstI C_TGCA'G
Cuts at: 830 2798 830
Size: 1968 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5446
PvuII CAG'CTG
Cuts at: 2699 2699
Size: 5446
SalI G'TCGA_C
Cuts at: 1936 2788 1936
Size: 852 4594
SanDI GG'GwC_CC
Cuts at: 2086 2706 2086
Size: 620 4826
SbfI CC_TGCA'GG
Cuts at: 2798 2798
Size: 5446
ScaI AGT'ACT
Cuts at: 3694 5406 3694
Size: 1712 3734
SfcI C'TryA_G
Cuts at: 826 1045 2249 2794 3929 4610 4801 826
Size: 219 1204 545 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 681 545 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5446
SmaI CCC'GGG
Cuts at: 1872 2773 1872
Size: 901 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2285 3550 4451 4713 820
Size: 197 926 342 1265 901 262 1553
Fragments arranged by size:
1553 1265 926 901 342 262 197
SnaBI TAC'GTA
Cuts at: 493 3237 493
Size: 2744 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5446
SphI G_CATG'C
Cuts at: 1132 2804 1132
Size: 1672 3774
SspI AAT'ATT
Cuts at: 5 52 2413 3370 5
Size: 47 2361 957 2081
Fragments arranged by size:
2361 2081 957 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5446
StyI C'CwwG_G
Cuts at: 513 1065 2004 2452 2673 2885 513
Size: 552 939 448 221 212 3074
Fragments arranged by size:
3074 939 552 448 221 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2198 2350 3473 3490 3643 3828 4273
Size: 1255 618 152 1123 17 153 185 445
Cuts at: 4273 325
Size: 1498
Fragments arranged by size:
1498 1255 1123 618 445 185 153 152
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3692 5404 96
Size: 274 80 33 51 1443 1715 1712 138
Fragments arranged by size:
1715 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5406 5438 5406
Size: 32 5414
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5446
XbaI T'CTAG_A
Cuts at: 2782 2782
Size: 5446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 5446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5446
XmaI C'CCGG_G
Cuts at: 1870 2771 1870
Size: 901 4545
XmnI GAAnn'nnTTC
Cuts at: 3575 3575
Size: 5446
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3254 276
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI