Restriction map for
pFN21AE6250
(Circular) (Six-base) MAPSORT of: pFN21AE6250.seq Check: 6559 from: 1 to: 5479
pFN21AE6250 5479 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2421 3289 278
Size: 53 83 186 936 885 868 2468
Fragments arranged by size:
2468 936 885 868 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3542 4926 5194 1632
Size: 1910 1384 268 1917
Fragments arranged by size:
1917 1910 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 2658 4378 5355 1203
Size: 1455 1720 977 1327
Fragments arranged by size:
1720 1455 1327 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AlwNI CAG_nnn'CTG
Cuts at: 3222 4794 3222
Size: 1572 3907
ApaI G_GGCC'C
Cuts at: 2017 2017
Size: 5479
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2788 3014 3166 5275 1493
Size: 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2166 2531 2804 1870
Size: 73 223 365 273 4545
Fragments arranged by size:
4545 365 273 223 73
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2800 4255 618
Size: 325 217 627 1013 1455 1842
Fragments arranged by size:
1842 1455 1013 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2017 2050 2165 2798 1391
Size: 626 33 115 633 4072
Fragments arranged by size:
4072 633 626 115 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3670 3704 1903
Size: 34 50 34 1649 34 3678
Fragments arranged by size:
3678 1649 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4090 136
Size: 107 122 71 631 605 2418 1525
Fragments arranged by size:
2418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2896 2896
Size: 5479
Bme1580I G_kGCm'C
Cuts at: 1790 2017 3540 4696 1790
Size: 227 1523 1156 2573
Fragments arranged by size:
2573 1523 1156 227
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4168 442
Size: 416 53 83 399 24 1793 958 1753
Fragments arranged by size:
1793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2415 4139 1239
Size: 1176 1724 2579
Fragments arranged by size:
2579 1724 1176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2212 4142 882
Size: 617 713 1930 2219
Fragments arranged by size:
2219 1930 713 617
BsaAI yAC'GTr
Cuts at: 493 1204 2566 3270 493
Size: 711 1362 704 2702
Fragments arranged by size:
2702 1362 711 704
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2418 2681 3286 3668
Size: 53 83 186 936 885 263 605 382
Cuts at: 3668 4303 275
Size: 635 1451
Fragments arranged by size:
1451 936 885 635 605 382 263 186
83 53
BsaWI w'CCGG_w
Cuts at: 3912 4310 4584 4731 5142 3912
Size: 398 274 147 411 4249
Fragments arranged by size:
4249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2602 2632 1178
Size: 30 165 30 1199 30 4025
Fragments arranged by size:
4025 1199 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2623 1186
Size: 1437 4042
BseYI C'CCAG_C
Cuts at: 2029 2063 2752 4682 5178 2029
Size: 34 689 1930 496 2330
Fragments arranged by size:
2330 1930 689 496 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 1163
Size: 573 426 4480
Fragments arranged by size:
4480 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3540 3625 4696 3540
Size: 85 1071 4323
Fragments arranged by size:
4323 1071 85
BsmI GAATG_Cn'
Cuts at: 2990 3083 2990
Size: 93 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2050 2165 2798 3540 3625 4696
Size: 399 227 33 115 633 742 85 1071
Cuts at: 4696 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 633 399 227 115 85
33
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2834 844
Size: 1990 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 1394
Size: 429 1543 3507
Fragments arranged by size:
3507 1543 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2703 3974 4148 65
Size: 1183 1455 1271 174 1396
Fragments arranged by size:
1455 1396 1271 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4123 4310 1700
Size: 110 2313 187 2869
Fragments arranged by size:
2869 2313 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 3539 4551 3539
Size: 1012 4467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 2170 1184
Size: 986 4493
BstXI CCAn_nnnn'nTGG
Cuts at: 2752 2752
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474
Size: 837 367 385 17 1452 86 12 346
Cuts at: 5474 1972
Size: 1977
Fragments arranged by size:
1977 1452 837 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2149 513
Size: 552 1084 3843
Fragments arranged by size:
3843 1084 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2179 2995 3789 3809 2179
Size: 816 794 20 3849
Fragments arranged by size:
3849 816 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2144 4486 809
Size: 507 828 2342 1802
Fragments arranged by size:
2342 1802 828 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2926 3815 5444
Size: 54 1425 50 275 18 1096 889 1629
Cuts at: 5444 8
Size: 43
Fragments arranged by size:
1629 1425 1096 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2098 3409 1353
Size: 740 5 1311 3423
Fragments arranged by size:
3423 1311 740 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 3542 4139 4926 5194 1239
Size: 393 783 1127 597 787 268 1524
Fragments arranged by size:
1524 1127 787 783 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2014 2313 2344 2014
Size: 299 31 5149
Fragments arranged by size:
5149 299 31
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 2236 4626 1678
Size: 558 2390 2531
Fragments arranged by size:
2531 2390 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2502 2534 4120
Size: 32 135 32 632 32 266 32 1586
Cuts at: 4120 4152 4194 4226 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1586 632 266 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 2658 5355 2658
Size: 2697 2782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4593 4777 1305
Size: 3288 184 2007
Fragments arranged by size:
3288 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3437 3796 3955 518
Size: 648 2271 359 159 2042
Fragments arranged by size:
2271 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2895 3572 4720 4965 1606
Size: 1289 677 1148 245 2120
Fragments arranged by size:
2120 1289 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2149 513
Size: 552 1084 3843
Fragments arranged by size:
3843 1084 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2837 4382 1132
Size: 97 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2202 1108
Size: 1094 4385
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2313 2344 2313
Size: 31 5448
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PspOMI G'GGCC_C
Cuts at: 2013 2013
Size: 5479
PstI C_TGCA'G
Cuts at: 830 2607 2831 830
Size: 1777 224 3478
Fragments arranged by size:
3478 1777 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SanDI GG'GwC_CC
Cuts at: 2344 2344
Size: 5479
SapI GCTCTTCn'nnn_
Cuts at: 2093 2093
Size: 5479
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SfcI C'TryA_G
Cuts at: 826 1045 2389 2603 2827 3962 4643 4834 826
Size: 219 1344 214 224 1135 681 191 1471
Fragments arranged by size:
1471 1344 1135 681 224 219 214 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2168 2806 1872
Size: 296 638 4545
Fragments arranged by size:
4545 638 296
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2149 2283 2745 2918 513
Size: 552 1084 134 462 173 3074
Fragments arranged by size:
3074 1084 552 462 173 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3506 3523 3676 3861 4306 325
Size: 1255 1926 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3725 5437 96
Size: 274 80 33 51 1443 1748 1712 138
Fragments arranged by size:
1748 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2602 2634 5439 5471 2602
Size: 32 2805 32 2610
Fragments arranged by size:
2805 2610 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 2071
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2166 2804 1870
Size: 296 638 4545
Fragments arranged by size:
4545 638 296
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2419 3287 276
Size: 53 83 186 936 885 868 2468
Fragments arranged by size:
2468 936 885 868 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI
SwaI