Restriction map for
pFN21AE6247
(Circular) (Six-base) MAPSORT of: pFN21AE6247.seq Check: 2651 from: 1 to: 5341
pFN21AE6247 5341 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2250 2250
Size: 5341
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 1937
Size: 747 4594
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3404 4788 5056 1632
Size: 1772 1384 268 1917
Fragments arranged by size:
1917 1772 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 2086 4070 2086
Size: 1984 3357
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 5309
AlwNI CAG_nnn'CTG
Cuts at: 2242 2470 2635 3084 4656 2242
Size: 228 165 449 1572 2927
Fragments arranged by size:
2927 1572 449 228 165
ApaLI G'TGCA_C
Cuts at: 2093 3398 4554 2093
Size: 1305 1156 2880
Fragments arranged by size:
2880 1305 1156
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 1870
Size: 73 723 4545
Fragments arranged by size:
4545 723 73
BamHI G'GATC_C
Cuts at: 2008 2671 3038 2008
Size: 663 367 4311
Fragments arranged by size:
4311 663 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2274 2662 4117 618
Size: 325 217 627 487 388 1455 1842
Fragments arranged by size:
1842 1455 627 487 388 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2660 1391
Size: 790 479 4072
Fragments arranged by size:
4072 790 479
BbeI G_GCGC'C
Cuts at: 2278 2278
Size: 5341
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3952 136
Size: 107 122 71 631 605 2280 1525
Fragments arranged by size:
2280 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5336 5336
Size: 5341
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2439 3402 4558 1790
Size: 307 342 963 1156 2573
Fragments arranged by size:
2573 1156 963 342 307
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 4030 442
Size: 416 53 83 399 24 1655 958 1753
Fragments arranged by size:
1753 1655 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2177 2378 4001 1239
Size: 851 87 201 1623 2579
Fragments arranged by size:
2579 1623 851 201 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3466 4331 4629 3466
Size: 865 298 4178
Fragments arranged by size:
4178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4004 882
Size: 617 2505 2219
Fragments arranged by size:
2505 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3132 493
Size: 711 1928 2702
Fragments arranged by size:
2702 1928 711
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2065 2275 3148 3530
Size: 53 83 186 936 532 210 873 382
Cuts at: 3530 4165 275
Size: 635 1451
Fragments arranged by size:
1451 936 873 635 532 382 210 186
83 53
BsaWI w'CCGG_w
Cuts at: 3774 4172 4446 4593 5004 3774
Size: 398 274 147 411 4111
Fragments arranged by size:
4111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 1178
Size: 30 165 30 747 30 4339
Fragments arranged by size:
4339 747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2377 1186
Size: 1191 4150
BseYI C'CCAG_C
Cuts at: 2518 4544 5040 2518
Size: 2026 496 2819
Fragments arranged by size:
2819 2026 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2648 1163
Size: 573 912 3856
Fragments arranged by size:
3856 912 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3552 4580 1540
Size: 275 186 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 3402 3487 4558 2097
Size: 1305 85 1071 2880
Fragments arranged by size:
2880 1305 1071 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
BsmBI CGTCTCn'nnnn_
Cuts at: 2066 2066
Size: 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2181 2439 2660 3402 3487 4558
Size: 399 307 84 258 221 742 85 1071
Cuts at: 4558 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 307 258 221 85
84
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2026 2250 2696 844
Size: 1182 224 446 3489
Fragments arranged by size:
3489 1182 446 224
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 1394
Size: 429 1405 3507
Fragments arranged by size:
3507 1405 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2505 3836 4010 65
Size: 1183 1257 1331 174 1396
Fragments arranged by size:
1396 1331 1257 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3985 4172 1700
Size: 110 2175 187 2869
Fragments arranged by size:
2869 2175 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 2042 2621 3401 4413 2042
Size: 579 780 1012 2970
Fragments arranged by size:
2970 1012 780 579
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2092 794
Size: 1298 4043
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 2518 2362
Size: 156 5185
BstYI r'GATC_y
Cuts at: 1972 2008 2046 2496 2671 3038 3423 3440 4892
Size: 36 38 450 175 367 385 17 1452
Cuts at: 4892 4978 4990 5336 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 450 385 367 346 175 86
38 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 2857 3651 3671 2372
Size: 485 794 20 4042
Fragments arranged by size:
4042 794 485 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2788 3677 5306
Size: 54 1425 50 275 18 958 889 1629
Cuts at: 5306 8
Size: 43
Fragments arranged by size:
1629 1425 958 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 1537
Size: 275 976 4090
Fragments arranged by size:
4090 976 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2304 3271 1353
Size: 951 967 3423
Fragments arranged by size:
3423 967 951
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2177 2378 3404 4001 4788 5056
Size: 393 458 87 201 1026 597 787 268
Cuts at: 5056 1239
Size: 1524
Fragments arranged by size:
1524 1026 787 597 458 393 268 201
87
EcoO109I rG'GnC_Cy
Cuts at: 2460 2460
Size: 5341
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 2278 4488 1678
Size: 600 2210 2531
Fragments arranged by size:
2531 2210 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2072 2104 2150
Size: 32 135 32 464 32 4 32 46
Cuts at: 2150 2182 2303 2335 3982 4014 4056 4088 1373
Size: 32 121 32 1647 32 42 32 2626
Fragments arranged by size:
2626 1647 464 135 121 46 42 32
32 32 32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2215 2685 2931 669
Size: 988 281 277 470 246 3079
Fragments arranged by size:
3079 988 470 281 277 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KasI G'GCGC_C
Cuts at: 2274 2274
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 4455 4639 1305
Size: 1248 1902 184 2007
Fragments arranged by size:
2007 1902 1248 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3519
Fragments arranged by size:
3519 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2537 3299 3658 3817 518
Size: 648 1194 177 762 359 159 2042
Fragments arranged by size:
2042 1194 762 648 359 177 159
MspA1I CmG'CkG
Cuts at: 1606 2757 3434 4582 4827 1606
Size: 1151 677 1148 245 2120
Fragments arranged by size:
2120 1151 1148 677 245
NarI GG'CG_CC
Cuts at: 2275 2275
Size: 5341
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2518 2699 4244 1132
Size: 97 1289 181 1545 2229
Fragments arranged by size:
2229 1545 1289 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5341
PfoI T'CCnGG_A
Cuts at: 2565 2565
Size: 5341
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2460 2460
Size: 5341
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2247 2379 2693 830
Size: 1417 132 314 3478
Fragments arranged by size:
3478 1417 314 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SapI GCTCTTCn'nnn_
Cuts at: 2304 2304
Size: 5341
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 2576 3589 5301 2576
Size: 1013 1712 2616
Fragments arranged by size:
2616 1712 1013
SfcI C'TryA_G
Cuts at: 826 1045 2243 2375 2689 3824 4505 4696 826
Size: 219 1198 132 314 1135 681 191 1471
Fragments arranged by size:
1471 1198 1135 681 314 219 191 132
SfoI GGC'GCC
Cuts at: 2276 2276
Size: 5341
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3445 4346 4608 820
Size: 197 926 1502 901 262 1553
Fragments arranged by size:
1553 1502 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 2443 3265 5
Size: 47 2391 822 2081
Fragments arranged by size:
2391 2081 822 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2004 2780 513
Size: 552 939 776 3074
Fragments arranged by size:
3074 939 776 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3368 3385 3538 3723 4168 325
Size: 1255 1788 17 153 185 445 1498
Fragments arranged by size:
1788 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 3587 5299
Size: 274 80 33 51 1443 597 1013 1712
Cuts at: 5299 96
Size: 138
Fragments arranged by size:
1712 1443 1013 597 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5301 5333 5301
Size: 32 5309
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2146 2677 2146
Size: 531 4810
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 2359
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 2313 3470 2313
Size: 1157 4184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI