Restriction map for
pFN21AE6244
(Circular) (Six-base) MAPSORT of: pFN21AE6244.seq Check: 2523 from: 1 to: 5539
pFN21AE6244 5539 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2671 2753 2671
Size: 82 5457
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2727 3349 278
Size: 53 83 186 936 1191 622 2468
Fragments arranged by size:
2468 1191 936 622 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2882 1937
Size: 945 4594
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2646 3602 4986 5254 1632
Size: 1014 956 1384 268 1917
Fragments arranged by size:
1917 1384 1014 956 268
AfeI AGC'GCT
Cuts at: 2345 2438 2345
Size: 93 5446
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5539
AlwNI CAG_nnn'CTG
Cuts at: 2686 3282 4854 2686
Size: 596 1572 3371
Fragments arranged by size:
3371 1572 596
ApaI G_GGCC'C
Cuts at: 2388 2388
Size: 5539
ApaLI G'TGCA_C
Cuts at: 3596 4752 3596
Size: 1156 4383
ApoI r'AATT_y
Cuts at: 1493 2848 3074 3226 5335 1493
Size: 1355 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2408 2516 2864 1870
Size: 73 465 108 348 4545
Fragments arranged by size:
4545 465 348 108 73
BamHI G'GATC_C
Cuts at: 2869 3236 2869
Size: 367 5172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2564 2593 2860 4315 618
Size: 325 217 627 777 29 267 1455 1842
Fragments arranged by size:
1842 1455 777 627 325 267 217 29
BanII G_rGCy'C
Cuts at: 1391 2232 2388 2858 1391
Size: 841 156 470 4072
Fragments arranged by size:
4072 841 470 156
BbeI G_GCGC'C
Cuts at: 2568 2568
Size: 5539
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2410 928
Size: 971 511 4057
Fragments arranged by size:
4057 971 511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 1903
Size: 34 1793 34 3678
Fragments arranged by size:
3678 1793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2163 4150 136
Size: 107 122 71 631 605 491 1987 1525
Fragments arranged by size:
1987 1525 631 605 491 122 107 71
BglII A'GATC_T
Cuts at: 5534 5534
Size: 5539
BlpI GC'TnA_GC
Cuts at: 2063 2956 2063
Size: 893 4646
Bme1580I G_kGCm'C
Cuts at: 1790 2388 2598 3600 4756 1790
Size: 598 210 1002 1156 2573
Fragments arranged by size:
2573 1156 1002 598 210
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5539
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 4228 442
Size: 416 53 83 399 24 1853 958 1753
Fragments arranged by size:
1853 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4199 1239
Size: 2960 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3664 4529 4827 3664
Size: 865 298 4376
Fragments arranged by size:
4376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3330 493
Size: 711 2126 2702
Fragments arranged by size:
2702 2126 711
BsaBI GATnn'nnATC
Cuts at: 5533 5533
Size: 5539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2415 2565 2724 3346
Size: 53 83 186 936 882 150 159 622
Cuts at: 3346 3728 4363 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 882 635 622 382 186 159
150 83 53
BsaWI w'CCGG_w
Cuts at: 2338 3972 4370 4644 4791 5202 2338
Size: 1634 398 274 147 411 2675
Fragments arranged by size:
2675 1634 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2617 1186
Size: 1431 4108
BseYI C'CCAG_C
Cuts at: 4742 5238 4742
Size: 496 5043
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2078 2372 2699 2846 1163
Size: 573 342 294 327 147 3856
Fragments arranged by size:
3856 573 342 327 294 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 2167 2989 3750 4778 1540
Size: 275 186 75 91 822 761 1028 2301
Fragments arranged by size:
2301 1028 822 761 275 186 91 75
BsiHKAI G_wGCw'C
Cuts at: 3600 3685 4756 3600
Size: 85 1071 4383
Fragments arranged by size:
4383 1071 85
BsmI GAATG_Cn'
Cuts at: 3050 3143 3050
Size: 93 5446
BsmBI CGTCTCn'nnnn_
Cuts at: 2293 2293
Size: 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2388 2598 2858 3600 3685 4756
Size: 399 442 156 210 260 742 85 1071
Cuts at: 4756 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 442 399 260 210 156
85
BspEI T'CCGG_A
Cuts at: 2338 5202 2338
Size: 2864 2675
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2553 2671 2753 2894 844
Size: 1709 118 82 141 3489
Fragments arranged by size:
3489 1709 141 118 82
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 1394
Size: 429 1603 3507
Fragments arranged by size:
3507 1603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2430 2830 4183 4370 1700
Size: 110 620 400 1353 187 2869
Fragments arranged by size:
2869 1353 620 400 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 2106 1925
Size: 181 5358
BssSI C'ACGA_G
Cuts at: 2735 3599 4611 2735
Size: 864 1012 3663
Fragments arranged by size:
3663 1012 864
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5539
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2508 2869 3236 3621 3638 5090 5176 5188
Size: 536 361 367 385 17 1452 86 12
Cuts at: 5188 5534 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 536 385 367 361 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2130 2158 2388 2607 513
Size: 552 939 24 102 28 230 219 3445
Fragments arranged by size:
3445 939 552 230 219 102 28 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 2445 2712 2808 3055 3849 3869 2445
Size: 267 96 247 794 20 4115
Fragments arranged by size:
4115 794 267 247 96 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2025 2073
Size: 54 1425 50 275 18 185 10 48
Cuts at: 2073 2164 2463 2986 3875 5504 8
Size: 91 299 523 889 1629 43
Fragments arranged by size:
1629 1425 889 523 299 275 185 91
54 50 48 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2073 2164 2986 1537
Size: 275 261 91 822 4090
Fragments arranged by size:
4090 822 275 261 91
EarI CTCTTCn'nnn_
Cuts at: 1353 2381 2623 3469 1353
Size: 1028 242 846 3423
Fragments arranged by size:
3423 1028 846 242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2116 2735 4126 4500 4646 387
Size: 1064 138 527 619 1391 374 146 1280
Fragments arranged by size:
1391 1280 1064 619 527 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2646 3602 4199 4986 5254 1239
Size: 393 1014 956 597 787 268 1524
Fragments arranged by size:
1524 1014 956 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2327 2327
Size: 5539
EcoO109I rG'GnC_Cy
Cuts at: 2248 2332 2384 2542 2248
Size: 84 52 158 5245
Fragments arranged by size:
5245 158 84 52
EcoRI G'AATT_C
Cuts at: 2848 3226 2848
Size: 378 5161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2584 2671 2584
Size: 87 5452
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2036 2347 2440 2568 4686 1678
Size: 358 311 93 128 2118 2531
Fragments arranged by size:
2531 2118 358 311 128 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4180 4212 4254 4286 1373
Size: 32 135 32 2608 32 42 32 2626
Fragments arranged by size:
2626 2608 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3129 669
Size: 988 281 945 246 3079
Fragments arranged by size:
3079 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 2209 748
Size: 1461 4078
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KasI G'GCGC_C
Cuts at: 2564 2564
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2316 4653 4837 1305
Size: 1011 2337 184 2007
Fragments arranged by size:
2337 2007 1011 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 2456 3497 3856 4015 518
Size: 648 1284 6 1041 359 159 2042
Fragments arranged by size:
2042 1284 1041 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2030 2062 2706 2955 3632 4780 5025 1606
Size: 424 32 644 249 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 644 424 249 245 32
NaeI GCC'GGC
Cuts at: 2832 2832
Size: 5539
NarI GG'CG_CC
Cuts at: 2565 2565
Size: 5539
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4048
Fragments arranged by size:
4048 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NgoMIV G'CCGG_C
Cuts at: 2830 2830
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2897 4442 1132
Size: 97 1668 1545 2229
Fragments arranged by size:
2229 1668 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5539
PfoI T'CCnGG_A
Cuts at: 2307 2307
Size: 5539
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2332 2542 2248
Size: 84 210 5245
Fragments arranged by size:
5245 210 84
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PspOMI G'GGCC_C
Cuts at: 2384 2384
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2265 2891 830
Size: 1435 626 3478
Fragments arranged by size:
3478 1435 626
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
PvuII CAG'CTG
Cuts at: 2062 2062
Size: 5539
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SanDI GG'GwC_CC
Cuts at: 2248 2248
Size: 5539
SapI GCTCTTCn'nnn_
Cuts at: 2381 2381
Size: 5539
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 2219 3787 5499 2219
Size: 1568 1712 2259
Fragments arranged by size:
2259 1712 1568
SfcI C'TryA_G
Cuts at: 826 1045 2261 2887 4022 4703 4894 826
Size: 219 1216 626 1135 681 191 1471
Fragments arranged by size:
1471 1216 1135 681 626 219 191
SfoI GGC'GCC
Cuts at: 2566 2566
Size: 5539
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2866 1872
Size: 994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3643 4544 4806 820
Size: 197 926 1700 901 262 1553
Fragments arranged by size:
1700 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5539
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2978 513
Size: 552 939 84 890 3074
Fragments arranged by size:
3074 939 890 552 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3566 3583 3736 3921 4366 325
Size: 1255 1986 17 153 185 445 1498
Fragments arranged by size:
1986 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 2217 2613
Size: 274 80 33 51 1443 123 117 396
Cuts at: 2613 3785 5497 96
Size: 1172 1712 138
Fragments arranged by size:
1712 1443 1172 396 274 138 123 117
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5539
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2458 2605 2458
Size: 147 5392
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2864 1870
Size: 994 4545
XmnI GAAnn'nnTTC
Cuts at: 2651 2654 3668 2651
Size: 3 1014 4522
Fragments arranged by size:
4522 1014 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2725 3347 276
Size: 53 83 186 936 1191 622 2468
Fragments arranged by size:
2468 1191 936 622 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SexAI SfiI SrfI SwaI