Restriction map for
pFN21AE6243
(Circular) (Six-base) MAPSORT of: pFN21AE6243.seq Check: 2417 from: 1 to: 5608
pFN21AE6243 5608 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2456 2929 2456
Size: 473 5135
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 3671 5055 5323 1632
Size: 786 1253 1384 268 1917
Fragments arranged by size:
1917 1384 1253 786 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 3351 4923 3351
Size: 1572 4036
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2917 3143 3295 5404 1493
Size: 1424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 1870
Size: 73 990 4545
Fragments arranged by size:
4545 990 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2440 2473 2440
Size: 33 5575
BamHI G'GATC_C
Cuts at: 2496 2938 3305 2496
Size: 442 367 4799
Fragments arranged by size:
4799 442 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2456 2929 4384 618
Size: 325 217 627 669 473 1455 1842
Fragments arranged by size:
1842 1455 669 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 2625 2927 1391
Size: 1234 302 4072
Fragments arranged by size:
4072 1234 302
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2192 928
Size: 971 293 4344
Fragments arranged by size:
4344 971 293
BbvCI CC'TCA_GC
Cuts at: 2762 2762
Size: 5608
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 1903
Size: 34 1862 34 3678
Fragments arranged by size:
3678 1862 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2846 4219 136
Size: 107 122 71 631 605 1174 1373 1525
Fragments arranged by size:
1525 1373 1174 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 2327 3025 2327
Size: 698 4910
Bme1580I G_kGCm'C
Cuts at: 1790 2632 3669 4825 1790
Size: 842 1037 1156 2573
Fragments arranged by size:
2573 1156 1037 842
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2683 3339 4297
Size: 416 53 83 399 24 1266 656 958
Cuts at: 4297 442
Size: 1753
Fragments arranged by size:
1753 1266 958 656 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2415 4268 1239
Size: 1176 1853 2579
Fragments arranged by size:
2579 1853 1176
Bpu10I CC'TnA_GC
Cuts at: 2762 2762
Size: 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2687 3733 4598 4896 2021
Size: 666 1046 865 298 2733
Fragments arranged by size:
2733 1046 865 666 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3797 4432 275
Size: 53 83 186 936 1882 382 635 1451
Fragments arranged by size:
1882 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2181 2599 4041 4439 4713 4860 5271 2181
Size: 418 1442 398 274 147 411 2518
Fragments arranged by size:
2518 1442 418 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2710 2740 1178
Size: 30 165 30 1307 30 4046
Fragments arranged by size:
4046 1307 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5608
BseYI C'CCAG_C
Cuts at: 2337 2351 2545 2648 2836 4811 5307 2337
Size: 14 194 103 188 1975 496 2638
Fragments arranged by size:
2638 1975 496 194 188 103 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2706 1163
Size: 573 970 4065
Fragments arranged by size:
4065 970 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2625 2703 3669 3754 4825 2328
Size: 297 78 966 85 1071 3111
Fragments arranged by size:
3111 1071 966 297 85 78
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2625 2632 2703 2927 3669 3754
Size: 399 538 297 7 71 224 742 85
Cuts at: 3754 4825 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 297 224 85
71 7
BspEI T'CCGG_A
Cuts at: 2181 2599 5271 2181
Size: 418 2672 2518
Fragments arranged by size:
2672 2518 418
BspHI T'CATG_A
Cuts at: 2385 2565 3497 5227 2385
Size: 180 932 1730 2766
Fragments arranged by size:
2766 1730 932 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 2963 844
Size: 1208 911 3489
Fragments arranged by size:
3489 1208 911
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 3495 1394
Size: 429 212 1460 3507
Fragments arranged by size:
3507 1460 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 2655 4103 4277 65
Size: 1183 1407 1448 174 1396
Fragments arranged by size:
1448 1407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 4252 4439 1700
Size: 110 241 2201 187 2869
Fragments arranged by size:
2869 2201 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 2799 96
Size: 2703 2905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 2087 1184
Size: 903 4705
BstXI CCAn_nnnn'nTGG
Cuts at: 2492 2492
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2496 2637 2664 2938 3305 3690 3707 5159
Size: 524 141 27 274 367 385 17 1452
Cuts at: 5159 5245 5257 5603 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 524 385 367 346 274 141
86 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4117
Fragments arranged by size:
4117 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 2823 3124 3918 3938 2823
Size: 301 794 20 4493
Fragments arranged by size:
4493 794 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2118 2153 529
Size: 35 1554 35 3984
Fragments arranged by size:
3984 1554 35 35
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2049 3055
Size: 54 1425 50 275 18 177 42 1006
Cuts at: 3055 3944 5573 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1006 889 275 177 54 50
43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3538 1353
Size: 2185 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2623 2623
Size: 5608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 2418 3671 4268 5055 5323 1239
Size: 393 783 3 1253 597 787 268 1524
Fragments arranged by size:
1524 1253 787 783 597 393 268 3
EcoO109I rG'GnC_Cy
Cuts at: 2166 2166
Size: 5608
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 4755 1678
Size: 3077 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2190 2222 2388 2420 2710
Size: 32 135 32 618 32 166 32 290
Cuts at: 2710 2742 4249 4281 4323 4355 1373
Size: 32 1507 32 42 32 2626
Fragments arranged by size:
2626 1507 618 290 166 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2460 2933 2460
Size: 473 5135
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2137 4722 4906 1305
Size: 832 2585 184 2007
Fragments arranged by size:
2585 2007 832 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2575 3566 3925 4084 518
Size: 648 1089 320 991 359 159 2042
Fragments arranged by size:
2042 1089 991 648 359 320 159
MspA1I CmG'CkG
Cuts at: 1606 2351 3024 3701 4849 5094 1606
Size: 745 673 677 1148 245 2120
Fragments arranged by size:
2120 1148 745 677 673 245
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 5608
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4117
Fragments arranged by size:
4117 939 552
NdeI CA'TA_TG
Cuts at: 387 2572 387
Size: 2185 3423
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2552 1108
Size: 1444 4164
PfoI T'CCnGG_A
Cuts at: 2392 2775 2392
Size: 383 5225
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2166 2166
Size: 5608
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 5576
PstI C_TGCA'G
Cuts at: 830 2121 2960 830
Size: 1291 839 3478
Fragments arranged by size:
3478 1291 839
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2351 2351
Size: 5608
SacI G_AGCT'C
Cuts at: 2625 2625
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2117 2956 4091 4772 4963 826
Size: 219 1072 839 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 839 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 2702 3712 4613 4875 820
Size: 197 926 93 666 1010 901 262 1553
Fragments arranged by size:
1553 1010 926 901 666 262 197 93
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2004 2460 2857 3047 513
Size: 552 939 456 397 190 3074
Fragments arranged by size:
3074 939 552 456 397 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 2328 3635 3652 3805 3990 4435
Size: 1255 495 253 1307 17 153 185 445
Cuts at: 4435 325
Size: 1498
Fragments arranged by size:
1498 1307 1255 495 445 253 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 2448 2799
Size: 274 80 33 51 1443 180 291 351
Cuts at: 2799 3854 5566 96
Size: 1055 1712 138
Fragments arranged by size:
1712 1443 1055 351 291 274 180 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 2102 1316
Size: 786 4822
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2902 2902
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI