Restriction map for
pFN21AE6242
(Circular) (Six-base) MAPSORT of: pFN21AE6242.seq Check: 7020 from: 1 to: 5536
pFN21AE6242 5536 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3346 278
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2879 1937
Size: 942 4594
Acc65I G'GTAC_C
Cuts at: 2354 2857 2354
Size: 503 5033
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 3599 4983 5251 1632
Size: 714 1253 1384 268 1917
Fragments arranged by size:
1917 1384 1253 714 268
AfeI AGC'GCT
Cuts at: 2028 2028
Size: 5536
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 4435 5412 1203
Size: 3232 977 1327
Fragments arranged by size:
3232 1327 977
AgeI A'CCGG_T
Cuts at: 4367 4367
Size: 5536
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5536
AlwNI CAG_nnn'CTG
Cuts at: 2152 2786 3279 4851 2152
Size: 634 493 1572 2837
Fragments arranged by size:
2837 1572 634 493
ApaLI G'TGCA_C
Cuts at: 2368 2552 3593 4749 2368
Size: 184 1041 1156 3155
Fragments arranged by size:
3155 1156 1041 184
ApoI r'AATT_y
Cuts at: 1493 2344 2845 3071 3223 5332 1493
Size: 851 501 226 152 2109 1697
Fragments arranged by size:
2109 1697 851 501 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2358 2861 1870
Size: 73 149 266 503 4545
Fragments arranged by size:
4545 503 266 149 73
BamHI G'GATC_C
Cuts at: 2622 2866 3233 2622
Size: 244 367 4925
Fragments arranged by size:
4925 367 244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2354 2857 4312 618
Size: 325 217 627 567 503 1455 1842
Fragments arranged by size:
1842 1455 627 567 503 325 217
BanII G_rGCy'C
Cuts at: 1391 2174 2443 2517 2735 2855 1391
Size: 783 269 74 218 120 4072
Fragments arranged by size:
4072 783 269 218 120 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4565
BbvCI CC'TCA_GC
Cuts at: 2787 2787
Size: 5536
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2349 2383 3727 3761 1903
Size: 34 412 34 1344 34 3678
Fragments arranged by size:
3678 1344 412 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2530 3428 4638 2530
Size: 898 1210 3428
Fragments arranged by size:
3428 1210 898
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4147 136
Size: 107 122 71 631 605 2475 1525
Fragments arranged by size:
2475 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5531 5531
Size: 5536
BlpI GC'TnA_GC
Cuts at: 2953 2953
Size: 5536
Bme1580I G_kGCm'C
Cuts at: 1790 2372 2556 3597 4753 1790
Size: 582 184 1041 1156 2573
Fragments arranged by size:
2573 1156 1041 582 184
BmgBI CAC'GTC
Cuts at: 1318 2101 2122 2260 1318
Size: 783 21 138 4594
Fragments arranged by size:
4594 783 138 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4225 442
Size: 416 53 83 399 24 1850 958 1753
Fragments arranged by size:
1850 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 4196 1239
Size: 819 2138 2579
Fragments arranged by size:
2579 2138 819
Bpu10I CC'TnA_GC
Cuts at: 2168 2787 2168
Size: 619 4917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3661 4526 4824 3661
Size: 865 298 4373
Fragments arranged by size:
4373 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2480 2744 4199 882
Size: 617 981 264 1455 2219
Fragments arranged by size:
2219 1455 981 617 264
BsaAI yAC'GTr
Cuts at: 493 1204 3327 493
Size: 711 2123 2702
Fragments arranged by size:
2702 2123 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2604 3343 3725 4360
Size: 53 83 186 936 1071 739 382 635
Cuts at: 4360 275
Size: 1451
Fragments arranged by size:
1451 1071 936 739 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3969 4367 4641 4788 5199 3969
Size: 398 274 147 411 4306
Fragments arranged by size:
4306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5311
Fragments arranged by size:
5311 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2516 1186
Size: 1327 3 4206
Fragments arranged by size:
4206 1327 3
BseYI C'CCAG_C
Cuts at: 2573 4739 5235 2573
Size: 2166 496 2874
Fragments arranged by size:
2874 2166 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2766 1163
Size: 573 1030 3933
Fragments arranged by size:
3933 1030 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2142 2986 3747 4775 1540
Size: 275 186 141 844 761 1028 2301
Fragments arranged by size:
2301 1028 844 761 275 186 141
BsiHKAI G_wGCw'C
Cuts at: 2109 2372 2556 3597 3682 4753 2109
Size: 263 184 1041 85 1071 2892
Fragments arranged by size:
2892 1071 1041 263 184 85
BsmI GAATG_Cn'
Cuts at: 2680 3047 3140 2680
Size: 367 93 5076
Fragments arranged by size:
5076 367 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2174 2372 2443 2517 2556 2735
Size: 399 319 65 198 71 74 39 179
Cuts at: 2735 2855 3597 3682 4753 1391
Size: 120 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 198 179 120
85 74 71 65 39
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 2427 2517 3425 5155 2427
Size: 90 908 1730 2808
Fragments arranged by size:
2808 1730 908 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 844
Size: 2047 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3423 1394
Size: 429 1600 3507
Fragments arranged by size:
3507 1600 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4031 4205 65
Size: 1183 2783 174 1396
Fragments arranged by size:
2783 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 4180 4367 1700
Size: 110 272 2098 187 2869
Fragments arranged by size:
2869 2098 272 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5536
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5536
BssSI C'ACGA_G
Cuts at: 3596 4608 3596
Size: 1012 4524
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5536
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5536
BstYI r'GATC_y
Cuts at: 1972 2622 2664 2866 3233 3618 3635 5087 5173
Size: 650 42 202 367 385 17 1452 86
Cuts at: 5173 5185 5531 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 650 385 367 346 202 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2797 513
Size: 552 1732 3252
Fragments arranged by size:
3252 1732 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2266 487
Size: 683 56 1040 3757
Fragments arranged by size:
3757 1040 683 56
BtsI GCAGTG_nn'
Cuts at: 2488 3052 3846 3866 2488
Size: 564 794 20 4158
Fragments arranged by size:
4158 794 564 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2264 4543 809
Size: 507 948 2279 1802
Fragments arranged by size:
2279 1802 948 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2139 2983
Size: 54 1425 50 275 18 231 78 844
Cuts at: 2983 3872 5501 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 844 275 231 78 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2139 2983 1537
Size: 275 327 844 4090
Fragments arranged by size:
4090 844 327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2813 3466 1353
Size: 1460 653 3423
Fragments arranged by size:
3423 1460 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 4123 4497 4643 387
Size: 1064 138 585 1949 374 146 1280
Fragments arranged by size:
1949 1280 1064 585 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2346 3599 4196 4983 5251 1239
Size: 393 426 288 1253 597 787 268 1524
Fragments arranged by size:
1524 1253 787 597 426 393 288 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2237 2237
Size: 5536
EcoO109I rG'GnC_Cy
Cuts at: 2164 2251 2714 2792 2164
Size: 87 463 78 4908
Fragments arranged by size:
4908 463 87 78
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FspI TGC'GCA
Cuts at: 2237 4042 2237
Size: 1805 3731
HaeII r_GCGC'y
Cuts at: 1678 2030 4683 1678
Size: 352 2653 2531
Fragments arranged by size:
2653 2531 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4177 4209 4251 4283 1373
Size: 32 135 32 2605 32 42 32 2626
Fragments arranged by size:
2626 2605 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2269 2880 3126 669
Size: 988 281 331 611 246 3079
Fragments arranged by size:
3079 988 611 331 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KpnI G_GTAC'C
Cuts at: 2358 2861 2358
Size: 503 5033
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4650 4834 1305
Size: 3345 184 2007
Fragments arranged by size:
3345 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2063 10
Size: 54 1425 343 231 3483
Fragments arranged by size:
3483 1425 343 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3494 3853 4012 518
Size: 648 2328 359 159 2042
Fragments arranged by size:
2328 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2152 2188 2952 3629 4777 5022 1606
Size: 546 36 764 677 1148 245 2120
Fragments arranged by size:
2120 1148 764 677 546 245 36
NaeI GCC'GGC
Cuts at: 2084 2084
Size: 5536
NcoI C'CATG_G
Cuts at: 513 1065 2797 513
Size: 552 1732 3252
Fragments arranged by size:
3252 1732 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NgoMIV G'CCGG_C
Cuts at: 2082 2082
Size: 5536
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2983 2983
Size: 5536
NspI r_CATG'y
Cuts at: 1132 1229 2894 4439 1132
Size: 97 1665 1545 2229
Fragments arranged by size:
2229 1665 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2687 1348
Size: 1339 4197
PciI A'CATG_T
Cuts at: 4435 4435
Size: 5536
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 1108
Size: 1080 4456
PfoI T'CCnGG_A
Cuts at: 2618 2618
Size: 5536
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2714 2792 2714
Size: 78 5458
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5536
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PstI C_TGCA'G
Cuts at: 830 2157 2208 2456 2469 2888 830
Size: 1327 51 248 13 419 3478
Fragments arranged by size:
3478 1327 419 248 51 13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
PvuII CAG'CTG
Cuts at: 2152 2188 2152
Size: 36 5500
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SanDI GG'GwC_CC
Cuts at: 2714 2792 2714
Size: 78 5458
SbfI CC_TGCA'GG
Cuts at: 2888 2888
Size: 5536
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SfcI C'TryA_G
Cuts at: 826 1045 2153 2204 2452 2465 2884 4019 4700
Size: 219 1108 51 248 13 419 1135 681
Cuts at: 4700 4891 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1108 681 419 248 219 191
51 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2863 1872
Size: 991 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3640 4541 4803 820
Size: 197 926 1697 901 262 1553
Fragments arranged by size:
1697 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2894 1132
Size: 1762 3774
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5536
StyI C'CwwG_G
Cuts at: 513 1065 2398 2718 2797 2975 513
Size: 552 1333 320 79 178 3074
Fragments arranged by size:
3074 1333 552 320 178 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2683 3563 3580 3733 3918 4363 325
Size: 1255 1103 880 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1103 880 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2760 3782 5494
Size: 274 80 33 51 1443 783 1022 1712
Cuts at: 5494 96
Size: 138
Fragments arranged by size:
1712 1443 1022 783 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2626 2658 5496 5528 2626
Size: 32 2838 32 2634
Fragments arranged by size:
2838 2634 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2861 1870
Size: 991 4545
XmnI GAAnn'nnTTC
Cuts at: 3665 3665
Size: 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3344 276
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI