Restriction map for
pFN21AE6240
(Circular) (Six-base) MAPSORT of: pFN21AE6240.seq Check: 308 from: 1 to: 5290
pFN21AE6240 5290 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3100 278
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2633 1937
Size: 696 4594
Acc65I G'GTAC_C
Cuts at: 2611 2611
Size: 5290
AclI AA'CG_TT
Cuts at: 1563 3417 3790 1563
Size: 1854 373 3063
Fragments arranged by size:
3063 1854 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 2537 3353 4737 5005 1632
Size: 797 108 816 1384 268 1917
Fragments arranged by size:
1917 1384 816 797 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5093
AflIII A'CryG_T
Cuts at: 1203 4189 5166 1203
Size: 2986 977 1327
Fragments arranged by size:
2986 1327 977
AgeI A'CCGG_T
Cuts at: 4121 4121
Size: 5290
AhdI GACnn_n'nnGTC
Cuts at: 2473 4019 2473
Size: 1546 3744
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2141 1184
Size: 957 4333
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2465 2497 2465
Size: 32 5258
AlwNI CAG_nnn'CTG
Cuts at: 3033 4605 3033
Size: 1572 3718
ApaI G_GGCC'C
Cuts at: 2331 2331
Size: 5290
ApaLI G'TGCA_C
Cuts at: 3347 4503 3347
Size: 1156 4134
ApoI r'AATT_y
Cuts at: 1493 2318 2599 2825 2977 5086 1493
Size: 825 281 226 152 2109 1697
Fragments arranged by size:
2109 1697 825 281 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3844 160
Size: 872 2812 1606
Fragments arranged by size:
2812 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
AvaI C'yCGr_G
Cuts at: 1870 1943 2330 2615 1870
Size: 73 387 285 4545
Fragments arranged by size:
4545 387 285 73
BamHI G'GATC_C
Cuts at: 2620 2987 2620
Size: 367 4923
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2611 4066 618
Size: 325 217 627 246 578 1455 1842
Fragments arranged by size:
1842 1455 627 578 325 246 217
BanII G_rGCy'C
Cuts at: 1391 2331 2535 2609 1391
Size: 940 204 74 4072
Fragments arranged by size:
4072 940 204 74
BbeI G_GCGC'C
Cuts at: 2037 2037
Size: 5290
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2278 2312 3481 3515 1903
Size: 34 341 34 1169 34 3678
Fragments arranged by size:
3678 1169 341 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3182 4392 3182
Size: 1210 4080
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5290
BfrBI ATG'CAT
Cuts at: 2366 2366
Size: 5290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2335 3901 136
Size: 107 122 71 631 605 663 1566 1525
Fragments arranged by size:
1566 1525 663 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5285 5285
Size: 5290
BlpI GC'TnA_GC
Cuts at: 2707 2707
Size: 5290
Bme1580I G_kGCm'C
Cuts at: 1790 2331 2578 3351 4507 1790
Size: 541 247 773 1156 2573
Fragments arranged by size:
2573 1156 773 541 247
BmgBI CAC'GTC
Cuts at: 1318 2085 1318
Size: 767 4523
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2479 3021 3979
Size: 416 53 83 399 24 1062 542 958
Cuts at: 3979 442
Size: 1753
Fragments arranged by size:
1753 1062 958 542 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 3950 1239
Size: 833 1878 2579
Fragments arranged by size:
2579 1878 833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 3415 4280 4578 2192
Size: 1223 865 298 2904
Fragments arranged by size:
2904 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3953 882
Size: 617 2454 2219
Fragments arranged by size:
2454 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2503 3081 493
Size: 711 1299 578 2702
Fragments arranged by size:
2702 1299 711 578
BsaBI GATnn'nnATC
Cuts at: 5284 5284
Size: 5290
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2253 3097 3479
Size: 53 83 186 936 501 219 844 382
Cuts at: 3479 4114 275
Size: 635 1451
Fragments arranged by size:
1451 936 844 635 501 382 219 186
83 53
BsaWI w'CCGG_w
Cuts at: 3723 4121 4395 4542 4953 3723
Size: 398 274 147 411 4060
Fragments arranged by size:
4060 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2465 2495 1178
Size: 30 165 30 1062 30 3973
Fragments arranged by size:
3973 1062 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 1186
Size: 1357 3933
BseYI C'CCAG_C
Cuts at: 2448 4493 4989 2448
Size: 2045 496 2749
Fragments arranged by size:
2749 2045 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4717
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2740 3501 4529 1540
Size: 275 186 739 761 1028 2301
Fragments arranged by size:
2301 1028 761 739 275 186
BsiHKAI G_wGCw'C
Cuts at: 3351 3436 4507 3351
Size: 85 1071 4134
Fragments arranged by size:
4134 1071 85
BsmI GAATG_Cn'
Cuts at: 2801 2894 2801
Size: 93 5197
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2535 2578 2609 3351 3436 4507
Size: 399 541 204 43 31 742 85 1071
Cuts at: 4507 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 541 399 204 85 43
31
BspEI T'CCGG_A
Cuts at: 4953 4953
Size: 5290
BspHI T'CATG_A
Cuts at: 3179 4909 3179
Size: 1730 3560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2106 2645 844
Size: 1262 539 3489
Fragments arranged by size:
3489 1262 539
BsrBI CCG'CTC
Cuts at: 1394 1823 2058 2092 3177 1394
Size: 429 235 34 1085 3507
Fragments arranged by size:
3507 1085 429 235 34
BsrDI GCAATG_nn'
Cuts at: 65 1248 3785 3959 65
Size: 1183 2537 174 1396
Fragments arranged by size:
2537 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2155 3934 4121 1700
Size: 110 345 1779 187 2869
Fragments arranged by size:
2869 1779 345 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5290
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5290
BssSI C'ACGA_G
Cuts at: 3350 4362 3350
Size: 1012 4278
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5290
BstBI TT'CG_AA
Cuts at: 4094 4094
Size: 5290
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5290
BstYI r'GATC_y
Cuts at: 1972 2620 2987 3372 3389 4841 4927 4939 5285
Size: 648 367 385 17 1452 86 12 346
Cuts at: 5285 1972
Size: 1977
Fragments arranged by size:
1977 1452 648 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3799
Fragments arranged by size:
3799 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4551
Fragments arranged by size:
4551 683 56
BtsI GCAGTG_nn'
Cuts at: 2439 2806 3600 3620 2439
Size: 367 794 20 4109
Fragments arranged by size:
4109 794 367 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5290
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5255
DraI TTT'AAA
Cuts at: 2594 2594
Size: 5290
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4297 809
Size: 507 2981 1802
Fragments arranged by size:
2981 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2277 2737
Size: 54 1425 50 275 18 330 117 460
Cuts at: 2737 3626 5255 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 460 330 275 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2737 1537
Size: 275 925 4090
Fragments arranged by size:
4090 925 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2353 3220 1353
Size: 1000 867 3423
Fragments arranged by size:
3423 1000 867
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3877 4251 4397 387
Size: 1064 138 2288 374 146 1280
Fragments arranged by size:
2288 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2429 2537 3353 3950 4737 5005
Size: 393 440 357 108 816 597 787 268
Cuts at: 5005 1239
Size: 1524
Fragments arranged by size:
1524 816 787 597 440 393 357 268
108
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2396
Size: 5290
EcoO109I rG'GnC_Cy
Cuts at: 2260 2374 2260
Size: 114 5176
EcoRI G'AATT_C
Cuts at: 2599 2977 2599
Size: 378 4912
FspI TGC'GCA
Cuts at: 3796 3796
Size: 5290
HaeII r_GCGC'y
Cuts at: 1678 2037 2245 4437 1678
Size: 359 208 2192 2531
Fragments arranged by size:
2531 2192 359 208
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 3931 3963 4005
Size: 32 135 32 887 32 1440 32 42
Cuts at: 4005 4037 1373
Size: 32 2626
Fragments arranged by size:
2626 1440 887 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2634 2880 669
Size: 988 281 696 246 3079
Fragments arranged by size:
3079 988 696 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5290
HpaI GTT'AAC
Cuts at: 2880 2880
Size: 5290
KasI G'GCGC_C
Cuts at: 2033 2033
Size: 5290
KpnI G_GTAC'C
Cuts at: 2615 2615
Size: 5290
MfeI C'AATT_G
Cuts at: 2889 2889
Size: 5290
MluI A'CGCG_T
Cuts at: 5166 5166
Size: 5290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4404 4588 1305
Size: 3099 184 2007
Fragments arranged by size:
3099 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 10
Size: 54 1425 343 447 3021
Fragments arranged by size:
3021 1425 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2273 3248 3607 3766 518
Size: 648 1107 975 359 159 2042
Fragments arranged by size:
2042 1107 975 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2166 2706 3383 4531 4776 1606
Size: 560 540 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 560 540 245
NaeI GCC'GGC
Cuts at: 2157 2157
Size: 5290
NarI GG'CG_CC
Cuts at: 2034 2034
Size: 5290
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3799
Fragments arranged by size:
3799 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5290
NgoMIV G'CCGG_C
Cuts at: 2155 2155
Size: 5290
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5290
NotI GC'GGCC_GC
Cuts at: 2737 2737
Size: 5290
NsiI A_TGCA'T
Cuts at: 2368 2368
Size: 5290
NspI r_CATG'y
Cuts at: 1132 1229 2648 4193 1132
Size: 97 1419 1545 2229
Fragments arranged by size:
2229 1545 1419 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5290
PciI A'CATG_T
Cuts at: 4189 4189
Size: 5290
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5290
PfoI T'CCnGG_A
Cuts at: 2256 2256
Size: 5290
PmeI GTTT'AAAC
Cuts at: 2594 2594
Size: 5290
PmlI CAC'GTG
Cuts at: 2503 2503
Size: 5290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2465 2497 3359 3391 4872 4904 2465
Size: 32 862 32 1481 32 2851
Fragments arranged by size:
2851 1481 862 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 2260
Size: 5290
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5290
PsiI TTA'TAA
Cuts at: 2860 2860
Size: 5290
PspOMI G'GGCC_C
Cuts at: 2327 2327
Size: 5290
PstI C_TGCA'G
Cuts at: 830 2642 830
Size: 1812 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5290
SalI G'TCGA_C
Cuts at: 1936 2632 1936
Size: 696 4594
SbfI CC_TGCA'GG
Cuts at: 2642 2642
Size: 5290
ScaI AGT'ACT
Cuts at: 3538 5250 3538
Size: 1712 3578
SfcI C'TryA_G
Cuts at: 826 1045 2638 3773 4454 4645 826
Size: 219 1593 1135 681 191 1471
Fragments arranged by size:
1593 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2335 2335
Size: 5290
SfoI GGC'GCC
Cuts at: 2035 2035
Size: 5290
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5290
SmaI CCC'GGG
Cuts at: 1872 2617 1872
Size: 745 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 3394 4295 4557 820
Size: 197 926 264 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 264 262 197
SnaBI TAC'GTA
Cuts at: 493 3081 493
Size: 2588 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5290
SphI G_CATG'C
Cuts at: 1132 2648 1132
Size: 1516 3774
SspI AAT'ATT
Cuts at: 5 52 3214 5
Size: 47 3162 2081
Fragments arranged by size:
3162 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5290
StyI C'CwwG_G
Cuts at: 513 1065 2004 2729 513
Size: 552 939 725 3074
Fragments arranged by size:
3074 939 725 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3317 3334 3487 3672 4117 325
Size: 1255 1737 17 153 185 445 1498
Fragments arranged by size:
1737 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2541 3536 5248
Size: 274 80 33 51 1443 564 995 1712
Cuts at: 5248 96
Size: 138
Fragments arranged by size:
1712 1443 995 564 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5250 5282 5250
Size: 32 5258
Tth111I GACn'n_nGTC
Cuts at: 1316 2252 1316
Size: 936 4354
XbaI T'CTAG_A
Cuts at: 2626 2626
Size: 5290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2389
Size: 5290
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5290
XmaI C'CCGG_G
Cuts at: 1870 2615 1870
Size: 745 4545
XmnI GAAnn'nnTTC
Cuts at: 3419 3419
Size: 5290
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3098 276
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI PacI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SrfI SwaI