Restriction map for
pFN21AE6239
(Circular) (Six-base) MAPSORT of: pFN21AE6239.seq Check: 3990 from: 1 to: 5560
pFN21AE6239 5560 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2823 2823
Size: 5560
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2903 1937
Size: 966 4594
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2868 3623 5007 5275 1632
Size: 1236 755 1384 268 1917
Fragments arranged by size:
1917 1384 1236 755 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5363
AflIII A'CryG_T
Cuts at: 1203 4459 5436 1203
Size: 3256 977 1327
Fragments arranged by size:
3256 1327 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 2594 1166
Size: 1428 4132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 2107 2221 2782 3303 4875 2107
Size: 114 561 521 1572 2792
Fragments arranged by size:
2792 1572 561 521 114
ApaI G_GGCC'C
Cuts at: 2122 2132 2547 2122
Size: 10 415 5135
Fragments arranged by size:
5135 415 10
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2869 3095 3247 5356 1493
Size: 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 1870
Size: 73 942 4545
Fragments arranged by size:
4545 942 73
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2881 4336 618
Size: 325 217 627 1094 1455 1842
Fragments arranged by size:
1842 1455 1094 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2042 2122 2132 2174 2415 2547 2879 1391
Size: 651 80 10 42 241 132 332 4072
Fragments arranged by size:
4072 651 332 241 132 80 42 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4589
BbvCI CC'TCA_GC
Cuts at: 2072 2072
Size: 5560
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2498 2532 3751 3785 1903
Size: 34 50 34 477 34 1219 34 3678
Fragments arranged by size:
3678 1219 477 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 136
Size: 107 122 71 631 605 2499 1525
Fragments arranged by size:
2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 2122 2132 2547 3621 4777 1790
Size: 332 10 415 1074 1156 2573
Fragments arranged by size:
2573 1156 1074 415 332 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2015 1056
Size: 959 4601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2390 2849 4220 1239
Size: 977 174 459 1371 2579
Fragments arranged by size:
2579 1371 977 459 174
Bpu10I CC'TnA_GC
Cuts at: 2072 2273 2634 2072
Size: 201 361 4998
Fragments arranged by size:
4998 361 201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 3685 4550 4848 2729
Size: 956 865 298 3441
Fragments arranged by size:
3441 956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4223 882
Size: 617 2724 2219
Fragments arranged by size:
2724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2145 2724 3351 493
Size: 711 941 579 627 2702
Fragments arranged by size:
2702 941 711 627 579
BsaBI GATnn'nnATC
Cuts at: 2093 5554 2093
Size: 3461 2099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3367 3749 4384 275
Size: 53 83 186 936 1834 382 635 1451
Fragments arranged by size:
1834 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2112 3993 4391 4665 4812 5223 2112
Size: 1881 398 274 147 411 2449
Fragments arranged by size:
2449 1881 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5335
Fragments arranged by size:
5335 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 1186
Size: 1072 4488
BseYI C'CCAG_C
Cuts at: 2033 4763 5259 2033
Size: 2730 496 2334
Fragments arranged by size:
2730 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4987
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2042 2174 2415 2664 3621 3706 4777 2042
Size: 132 241 249 957 85 1071 2825
Fragments arranged by size:
2825 1071 957 249 241 132 85
BsmI GAATG_Cn'
Cuts at: 3071 3164 3071
Size: 93 5467
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2122 2132 2174 2415 2547 2664
Size: 399 252 80 10 42 241 132 117
Cuts at: 2664 2879 3621 3706 4777 1391
Size: 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 252 241 215 132
117 85 80 42 10
BspEI T'CCGG_A
Cuts at: 5223 5223
Size: 5560
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2823 2915 844
Size: 1979 92 3489
Fragments arranged by size:
3489 1979 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3447 1394
Size: 429 1624 3507
Fragments arranged by size:
3507 1624 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2439 4055 4229 65
Size: 1183 1191 1616 174 1396
Fragments arranged by size:
1616 1396 1191 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4204 4391 1700
Size: 110 2394 187 2869
Fragments arranged by size:
2869 2394 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 2414 2507 3620 4632 2414
Size: 93 1113 1012 3342
Fragments arranged by size:
3342 1113 1012 93
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5560
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstXI CCAn_nnnn'nTGG
Cuts at: 2677 2677
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2890 3257 3642 3659 5111 5197 5209 5555
Size: 918 367 385 17 1452 86 12 346
Cuts at: 5555 1972
Size: 1977
Fragments arranged by size:
1977 1452 918 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2391 513
Size: 552 1326 3682
Fragments arranged by size:
3682 1326 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 3076 3870 3890 3076
Size: 794 20 4746
Fragments arranged by size:
4746 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2610 3007
Size: 54 1425 50 275 18 261 519 397
Cuts at: 3007 3896 5525 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 519 397 275 261 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2403 3490 1353
Size: 837 213 1087 3423
Fragments arranged by size:
3423 1087 837 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2182 2672 4147 4521 4667 387
Size: 1064 138 593 490 1475 374 146 1280
Fragments arranged by size:
1475 1280 1064 593 490 374 146 138
EcoICRI GAG'CTC
Cuts at: 2040 2172 2413 2040
Size: 132 241 5187
Fragments arranged by size:
5187 241 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2390 2849 2868 3623 4220 5007
Size: 393 584 174 459 19 755 597 787
Cuts at: 5007 5275 1239
Size: 268 1524
Fragments arranged by size:
1524 787 755 597 584 459 393 268
174 19
EcoO109I rG'GnC_Cy
Cuts at: 2118 2119 2128 2129 2470 2543 2544 2779 2118
Size: 1 9 1 341 73 1 235 4899
Fragments arranged by size:
4899 341 235 73 9 1 1 1
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 2057 2819 4066 2057
Size: 762 1247 3551
Fragments arranged by size:
3551 1247 762
HaeII r_GCGC'y
Cuts at: 1678 2581 2827 4707 1678
Size: 903 246 1880 2531
Fragments arranged by size:
2531 1880 903 246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4201 4233 4275 4307 1373
Size: 32 135 32 2629 32 42 32 2626
Fragments arranged by size:
2629 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2904 3150 669
Size: 988 281 966 246 3079
Fragments arranged by size:
3079 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4674 4858 1305
Size: 3369 184 2007
Fragments arranged by size:
3369 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 2612 10
Size: 54 1425 343 261 519 2958
Fragments arranged by size:
2958 1425 519 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2594 2675 3518 3877 4036 518
Size: 648 1428 81 843 359 159 2042
Fragments arranged by size:
2042 1428 843 648 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 2976 3653 4801 5046 1606
Size: 1370 677 1148 245 2120
Fragments arranged by size:
2120 1370 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2391 513
Size: 552 1326 3682
Fragments arranged by size:
3682 1326 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 2011 1052
Size: 959 4601
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2056 2632 2918 4463 1132
Size: 97 827 576 286 1545 2229
Fragments arranged by size:
2229 1545 827 576 286 97
PasI CC'CwG_GG
Cuts at: 1348 2122 1348
Size: 774 4786
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2221 2392 1108
Size: 1113 171 4276
Fragments arranged by size:
4276 1113 171
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PmlI CAC'GTG
Cuts at: 2145 2145
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2470 2779 2470
Size: 309 5251
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PspOMI G'GGCC_C
Cuts at: 2118 2128 2543 2118
Size: 10 415 5135
Fragments arranged by size:
5135 415 10
PstI C_TGCA'G
Cuts at: 830 2478 2912 830
Size: 1648 434 3478
Fragments arranged by size:
3478 1648 434
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
SacI G_AGCT'C
Cuts at: 2042 2174 2415 2042
Size: 132 241 5187
Fragments arranged by size:
5187 241 132
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SapI GCTCTTCn'nnn_
Cuts at: 2403 2403
Size: 5560
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SexAI A'CCwGG_T
Cuts at: 2154 2535 2775 2154
Size: 381 240 4939
Fragments arranged by size:
4939 381 240
SfcI C'TryA_G
Cuts at: 826 1045 2044 2206 2474 2908 4043 4724 4915
Size: 219 999 162 268 434 1135 681 191
Cuts at: 4915 826
Size: 1471
Fragments arranged by size:
1471 1135 999 681 434 268 219 191
162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 3664 4565 4827 820
Size: 197 926 801 920 901 262 1553
Fragments arranged by size:
1553 926 920 901 801 262 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 2261 1517
Size: 744 4816
StyI C'CwwG_G
Cuts at: 513 1065 2262 2391 2999 513
Size: 552 1197 129 608 3074
Fragments arranged by size:
3074 1197 608 552 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3587 3604 3757 3942 4387 325
Size: 1255 2007 17 153 185 445 1498
Fragments arranged by size:
2007 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3806 5518 96
Size: 274 80 33 51 1443 1829 1712 138
Fragments arranged by size:
1829 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2523 2555 5520 5552 2523
Size: 32 2965 32 2531
Fragments arranged by size:
2965 2531 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 2596
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 3689 3689
Size: 5560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspXI PsrI PvuII RsrII SacII SanDI
SfiI SfoI SrfI SwaI