Restriction map for
pFN21AE6238
(Circular) (Six-base) MAPSORT of: pFN21AE6238.seq Check: 8992 from: 1 to: 5614
pFN21AE6238 5614 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3677 5061 5329 1632
Size: 2045 1384 268 1917
Fragments arranged by size:
2045 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 4513 5490 1203
Size: 3310 977 1327
Fragments arranged by size:
3310 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AlwNI CAG_nnn'CTG
Cuts at: 3357 4929 3357
Size: 1572 4042
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2923 3149 3301 5410 1493
Size: 1430 226 152 2109 1697
Fragments arranged by size:
2109 1697 1430 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2379 2939 1870
Size: 73 436 560 4545
Fragments arranged by size:
4545 560 436 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2935 4390 618
Size: 325 217 627 1148 1455 1842
Fragments arranged by size:
1842 1455 1148 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2039 2933 1391
Size: 648 894 4072
Fragments arranged by size:
4072 894 648
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2888 928
Size: 971 989 3654
Fragments arranged by size:
3654 989 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 2433 1548
Size: 885 4729
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4225 136
Size: 107 122 71 631 605 2553 1525
Fragments arranged by size:
2553 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2444 5609 2444
Size: 3165 2449
BlpI GC'TnA_GC
Cuts at: 2178 3031 2178
Size: 853 4761
Bme1580I G_kGCm'C
Cuts at: 1790 3675 4831 1790
Size: 1885 1156 2573
Fragments arranged by size:
2573 1885 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4303 442
Size: 416 53 83 399 24 1928 958 1753
Fragments arranged by size:
1928 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2685 2685
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4274 1239
Size: 3035 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2655 3739 4604 4902 2655
Size: 1084 865 298 3367
Fragments arranged by size:
3367 1084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2048 2168 4277 882
Size: 617 549 120 2109 2219
Fragments arranged by size:
2219 2109 617 549 120
BsaAI yAC'GTr
Cuts at: 493 1204 2074 3405 493
Size: 711 870 1331 2702
Fragments arranged by size:
2702 1331 870 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2039 2862 4047 4445 4719 4866 5277 2039
Size: 823 1185 398 274 147 411 2376
Fragments arranged by size:
2376 1185 823 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 1186
Size: 1138 4476
BseYI C'CCAG_C
Cuts at: 2058 4817 5313 2058
Size: 2759 496 2359
Fragments arranged by size:
2759 2359 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5041
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 2085 3675 3760 4831 2031
Size: 54 1590 85 1071 2814
Fragments arranged by size:
2814 1590 1071 85 54
BsmI GAATG_Cn'
Cuts at: 2526 3125 3218 2526
Size: 599 93 4922
Fragments arranged by size:
4922 599 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2039 2085 2933 3675 3760 4831
Size: 399 241 8 46 848 742 85 1071
Cuts at: 4831 1391
Size: 2174
Fragments arranged by size:
2174 1071 848 742 399 241 85 46
8
BspEI T'CCGG_A
Cuts at: 2862 5277 2862
Size: 2415 3199
BspHI T'CATG_A
Cuts at: 3503 5233 3503
Size: 1730 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2969 844
Size: 2125 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2065 3501 1394
Size: 429 242 1436 3507
Fragments arranged by size:
3507 1436 429 242
BsrDI GCAATG_nn'
Cuts at: 65 1248 2184 4109 4283 65
Size: 1183 936 1925 174 1396
Fragments arranged by size:
1925 1396 1183 936 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4258 4445 1700
Size: 110 2448 187 2869
Fragments arranged by size:
2869 2448 187 110
BsrGI T'GTAC_A
Cuts at: 96 2329 96
Size: 2233 3381
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 3674 4686 3674
Size: 1012 4602
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5614
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2734
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2444 2944 3311 3696 3713 5165 5251 5263
Size: 472 500 367 385 17 1452 86 12
Cuts at: 5263 5609 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 500 472 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2266 513
Size: 552 939 262 3861
Fragments arranged by size:
3861 939 552 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 3924 3944 3130
Size: 794 20 4800
Fragments arranged by size:
4800 794 20
ClaI AT'CG_AT
Cuts at: 1554 2196 1554
Size: 642 4972
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2464 2499 2731 2766 529
Size: 35 1900 35 232 35 3377
Fragments arranged by size:
3377 1900 232 35 35 35
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 3061 3950
Size: 54 1425 50 275 18 186 1045 889
Cuts at: 3950 5579 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1045 889 275 186 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2514 2654 3544 1353
Size: 759 402 140 890 3423
Fragments arranged by size:
3423 890 759 402 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4201 4575 4721 387
Size: 1064 138 435 2177 374 146 1280
Fragments arranged by size:
2177 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3677 4274 5061 5329 1239
Size: 393 2045 597 787 268 1524
Fragments arranged by size:
2045 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2318 2869 2318
Size: 551 5063
EcoRI G'AATT_C
Cuts at: 2923 3301 2923
Size: 378 5236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 5614
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 4761 1678
Size: 3083 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2373 2377 2405 2409 4255
Size: 32 135 32 801 4 28 4 1846
Cuts at: 4255 4287 4329 4361 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1846 801 135 42 32 32 32
32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 2743 2958 3204 669
Size: 988 281 765 40 215 246 3079
Fragments arranged by size:
3079 988 765 281 246 215 40
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2138 4728 4912 1305
Size: 833 2590 184 2007
Fragments arranged by size:
2590 2007 833 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3792
Fragments arranged by size:
3792 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2090 3572 3931 4090 518
Size: 648 906 18 1482 359 159 2042
Fragments arranged by size:
2042 1482 906 648 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 3030 3707 4855 5100 1606
Size: 1424 677 1148 245 2120
Fragments arranged by size:
2120 1424 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2266 513
Size: 552 939 262 3861
Fragments arranged by size:
3861 939 552 262
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2818 2972 4517 1132
Size: 97 1589 154 1545 2229
Fragments arranged by size:
2229 1589 1545 154 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5614
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2318 2869 2318
Size: 551 5063
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2966 830
Size: 2136 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 2480 3862 5574 2480
Size: 1382 1712 2520
Fragments arranged by size:
2520 1712 1382
SfcI C'TryA_G
Cuts at: 826 1045 2962 4097 4778 4969 826
Size: 219 1917 1135 681 191 1471
Fragments arranged by size:
1917 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2379 2670 3718 4619 4881 820
Size: 197 926 436 291 1048 901 262 1553
Fragments arranged by size:
1553 1048 926 901 436 291 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2818 2972 1132
Size: 1686 154 3774
Fragments arranged by size:
3774 1686 154
SspI AAT'ATT
Cuts at: 5 52 2131 3538 5
Size: 47 2079 1407 2081
Fragments arranged by size:
2081 2079 1407 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2004 2251 2266 3053 513
Size: 552 939 247 15 787 3074
Fragments arranged by size:
3074 939 787 552 247 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3641 3658 3811 3996 4441 325
Size: 1255 2061 17 153 185 445 1498
Fragments arranged by size:
2061 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2329 2478 3860
Size: 274 80 33 51 1443 352 149 1382
Cuts at: 3860 5572 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1382 352 274 149 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2409 2441 5574 5606 2409
Size: 32 3133 32 2417
Fragments arranged by size:
3133 2417 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 2379 1943
Size: 436 5178
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI