Restriction map for
pFN21AE6235
(Circular) (Six-base) MAPSORT of: pFN21AE6235.seq Check: 2716 from: 1 to: 5452
pFN21AE6235 5452 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3262 278
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2795 1937
Size: 858 4594
Acc65I G'GTAC_C
Cuts at: 2773 2773
Size: 5452
AclI AA'CG_TT
Cuts at: 1563 3579 3952 1563
Size: 2016 373 3063
Fragments arranged by size:
3063 2016 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3515 4899 5167 1632
Size: 1883 1384 268 1917
Fragments arranged by size:
1917 1883 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5255
AflIII A'CryG_T
Cuts at: 1203 2243 4351 5328 1203
Size: 1040 2108 977 1327
Fragments arranged by size:
2108 1327 1040 977
AgeI A'CCGG_T
Cuts at: 4283 4283
Size: 5452
AhdI GACnn_n'nnGTC
Cuts at: 4181 4181
Size: 5452
AleI CACnn'nnGTG
Cuts at: 1166 2093 1166
Size: 927 4525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5452
AlwNI CAG_nnn'CTG
Cuts at: 2526 3195 4767 2526
Size: 669 1572 3211
Fragments arranged by size:
3211 1572 669
ApaLI G'TGCA_C
Cuts at: 3509 4665 3509
Size: 1156 4296
ApoI r'AATT_y
Cuts at: 1493 2761 2987 3139 5248 1493
Size: 1268 226 152 2109 1697
Fragments arranged by size:
2109 1697 1268 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4006 160
Size: 872 2974 1606
Fragments arranged by size:
2974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
AvaI C'yCGr_G
Cuts at: 1870 1943 2777 1870
Size: 73 834 4545
Fragments arranged by size:
4545 834 73
BamHI G'GATC_C
Cuts at: 2782 3149 2782
Size: 367 5085
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2201 2773 4228 618
Size: 325 217 627 351 63 572 1455 1842
Fragments arranged by size:
1842 1455 627 572 351 325 217 63
BanII G_rGCy'C
Cuts at: 1391 2313 2771 1391
Size: 922 458 4072
Fragments arranged by size:
4072 922 458
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2071 928
Size: 971 172 4309
Fragments arranged by size:
4309 971 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3643 3677 1903
Size: 34 1706 34 3678
Fragments arranged by size:
3678 1706 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4554 3344
Size: 1210 4242
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4063 136
Size: 107 122 71 631 605 2391 1525
Fragments arranged by size:
2391 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5447 5447
Size: 5452
BlpI GC'TnA_GC
Cuts at: 2307 2869 2307
Size: 562 4890
Bme1580I G_kGCm'C
Cuts at: 1790 2141 3513 4669 1790
Size: 351 1372 1156 2573
Fragments arranged by size:
2573 1372 1156 351
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 2677 3183
Size: 416 53 83 399 24 683 577 506
Cuts at: 3183 4141 442
Size: 958 1753
Fragments arranged by size:
1753 958 683 577 506 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2500 2522 2676 4112 1239
Size: 1261 22 154 1436 2579
Fragments arranged by size:
2579 1436 1261 154 22
Bpu10I CC'TnA_GC
Cuts at: 2447 2447
Size: 5452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3577 4442 4740 3577
Size: 865 298 4289
Fragments arranged by size:
4289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 4115 882
Size: 617 519 2097 2219
Fragments arranged by size:
2219 2097 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 3243 493
Size: 711 2039 2702
Fragments arranged by size:
2702 2039 711
BsaBI GATnn'nnATC
Cuts at: 2547 5446 2547
Size: 2899 2553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3259 3641 4276 275
Size: 53 83 186 936 1726 382 635 1451
Fragments arranged by size:
1726 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3885 4283 4557 4704 5115 3885
Size: 398 274 147 411 4222
Fragments arranged by size:
4222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2161 2191 1178
Size: 30 165 30 758 30 4439
Fragments arranged by size:
4439 758 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 2048 2428 1186
Size: 843 19 380 4210
Fragments arranged by size:
4210 843 380 19
BseYI C'CCAG_C
Cuts at: 2519 4655 5151 2519
Size: 2136 496 2820
Fragments arranged by size:
2820 2136 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 2483 1163
Size: 573 720 27 4132
Fragments arranged by size:
4132 720 573 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2902 3663 4691 1540
Size: 275 186 901 761 1028 2301
Fragments arranged by size:
2301 1028 901 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2313 2486 3513 3598 4669 2190
Size: 123 173 1027 85 1071 2973
Fragments arranged by size:
2973 1071 1027 173 123 85
BsmI GAATG_Cn'
Cuts at: 2675 2963 3056 2675
Size: 288 93 5071
Fragments arranged by size:
5071 288 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2190 2313 2486 2771 3513 3598
Size: 399 351 49 123 173 285 742 85
Cuts at: 3598 4669 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 351 285 173 123
85 49
BspEI T'CCGG_A
Cuts at: 5115 5115
Size: 5452
BspHI T'CATG_A
Cuts at: 2344 3341 5071 2344
Size: 997 1730 2725
Fragments arranged by size:
2725 1730 997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2807 844
Size: 1963 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3339 1394
Size: 429 1516 3507
Fragments arranged by size:
3507 1516 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2410 3947 4121 65
Size: 1183 1162 1537 174 1396
Fragments arranged by size:
1537 1396 1183 1162 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4096 4283 1700
Size: 110 2286 187 2869
Fragments arranged by size:
2869 2286 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5452
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5452
BssSI C'ACGA_G
Cuts at: 3512 4524 3512
Size: 1012 4440
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5452
BstBI TT'CG_AA
Cuts at: 4256 4256
Size: 5452
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5452
BstXI CCAn_nnnn'nTGG
Cuts at: 2690 2690
Size: 5452
BstYI r'GATC_y
Cuts at: 1972 2557 2782 3149 3534 3551 5003 5089 5101
Size: 585 225 367 385 17 1452 86 12
Cuts at: 5101 5447 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 585 385 367 346 225 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2366 2366
Size: 5452
BtgI C'CryG_G
Cuts at: 513 1065 2004 2258 513
Size: 552 939 254 3707
Fragments arranged by size:
3707 939 552 254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2635 487
Size: 683 56 1409 3304
Fragments arranged by size:
3304 1409 683 56
BtsI GCAGTG_nn'
Cuts at: 2430 2968 3762 3782 2430
Size: 538 794 20 4100
Fragments arranged by size:
4100 794 538 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2194 2229 529
Size: 35 1630 35 3752
Fragments arranged by size:
3752 1630 35 35
DraI TTT'AAA
Cuts at: 2756 2756
Size: 5452
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4459 809
Size: 507 3143 1802
Fragments arranged by size:
3143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2577 2730 2899
Size: 54 1425 50 275 18 747 153 169
Cuts at: 2899 3788 5417 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 747 275 169 153 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2899 1537
Size: 275 1087 4090
Fragments arranged by size:
4090 1087 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3382 1353
Size: 2029 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4039 4413 4559 387
Size: 1064 138 2450 374 146 1280
Fragments arranged by size:
2450 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 2311
Size: 5452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2500 2522 2676 3515 4112 4899 5167
Size: 393 868 22 154 839 597 787 268
Cuts at: 5167 1239
Size: 1524
Fragments arranged by size:
1524 868 839 787 597 393 268 154
22
EcoNI CCTnn'n_nnAGG
Cuts at: 2365 2365
Size: 5452
EcoRI G'AATT_C
Cuts at: 2761 3139 2761
Size: 378 5074
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 5452
FspI TGC'GCA
Cuts at: 3958 3958
Size: 5452
HaeII r_GCGC'y
Cuts at: 1678 4599 1678
Size: 2921 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 4093 4125 4167
Size: 32 135 32 457 32 2032 32 42
Cuts at: 4167 4199 1373
Size: 32 2626
Fragments arranged by size:
2626 2032 457 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2593 2796 3042 669
Size: 988 281 655 203 246 3079
Fragments arranged by size:
3079 988 655 281 246 203
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5452
HpaI GTT'AAC
Cuts at: 2593 3042 2593
Size: 449 5003
KpnI G_GTAC'C
Cuts at: 2777 2777
Size: 5452
MfeI C'AATT_G
Cuts at: 3051 3051
Size: 5452
MluI A'CGCG_T
Cuts at: 5328 5328
Size: 5452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2092 4566 4750 1305
Size: 787 2474 184 2007
Fragments arranged by size:
2474 2007 787 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3630
Fragments arranged by size:
3630 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 3410 3769 3928 518
Size: 648 927 1317 359 159 2042
Fragments arranged by size:
2042 1317 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2523 2868 3545 4693 4938 1606
Size: 917 345 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 345 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2258 513
Size: 552 939 254 3707
Fragments arranged by size:
3707 939 552 254
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5452
NotI GC'GGCC_GC
Cuts at: 2899 2899
Size: 5452
NspI r_CATG'y
Cuts at: 1132 1229 2247 2698 2810 4355 1132
Size: 97 1018 451 112 1545 2229
Fragments arranged by size:
2229 1545 1018 451 112 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5452
PciI A'CATG_T
Cuts at: 2243 4351 2243
Size: 2108 3344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5452
PfoI T'CCnGG_A
Cuts at: 2499 2499
Size: 5452
PmeI GTTT'AAAC
Cuts at: 2756 2756
Size: 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 5034 5066 3521
Size: 32 1481 32 3907
Fragments arranged by size:
3907 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5452
PsiI TTA'TAA
Cuts at: 3022 3022
Size: 5452
PstI C_TGCA'G
Cuts at: 830 2804 830
Size: 1974 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5452
PvuII CAG'CTG
Cuts at: 2523 2523
Size: 5452
SacI G_AGCT'C
Cuts at: 2313 2313
Size: 5452
SalI G'TCGA_C
Cuts at: 1936 2794 1936
Size: 858 4594
SbfI CC_TGCA'GG
Cuts at: 2804 2804
Size: 5452
ScaI AGT'ACT
Cuts at: 3700 5412 3700
Size: 1712 3740
SfcI C'TryA_G
Cuts at: 826 1045 2800 3935 4616 4807 826
Size: 219 1755 1135 681 191 1471
Fragments arranged by size:
1755 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5452
SmaI CCC'GGG
Cuts at: 1872 2779 1872
Size: 907 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3556 4457 4719 820
Size: 197 926 1613 901 262 1553
Fragments arranged by size:
1613 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3243 493
Size: 2750 2702
SpeI A'CTAG_T
Cuts at: 152 2621 152
Size: 2469 2983
SphI G_CATG'C
Cuts at: 1132 2698 2810 1132
Size: 1566 112 3774
Fragments arranged by size:
3774 1566 112
SspI AAT'ATT
Cuts at: 5 52 3376 5
Size: 47 3324 2081
Fragments arranged by size:
3324 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5452
StyI C'CwwG_G
Cuts at: 513 1065 2004 2258 2891 513
Size: 552 939 254 633 3074
Fragments arranged by size:
3074 939 633 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3479 3496 3649 3834 4279 325
Size: 1255 1899 17 153 185 445 1498
Fragments arranged by size:
1899 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3698 5410 96
Size: 274 80 33 51 1443 1721 1712 138
Fragments arranged by size:
1721 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2161 2193 5412 5444 2161
Size: 32 3219 32 2169
Fragments arranged by size:
3219 2169 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5452
XbaI T'CTAG_A
Cuts at: 2788 2788
Size: 5452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5452
XmaI C'CCGG_G
Cuts at: 1870 2777 1870
Size: 907 4545
XmnI GAAnn'nnTTC
Cuts at: 3581 3581
Size: 5452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3260 276
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI