Restriction map for
pFN21AE6230
(Circular) (Six-base) MAPSORT of: pFN21AE6230.seq Check: 4608 from: 1 to: 5605
pFN21AE6230 5605 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2244 2948 1937
Size: 307 704 4594
Fragments arranged by size:
4594 704 307
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2331 2843 3668 5052 5320 1632
Size: 699 512 825 1384 268 1917
Fragments arranged by size:
1917 1384 825 699 512 268
AfeI AGC'GCT
Cuts at: 2177 2177
Size: 5605
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 2239 2427 4504 5481 1203
Size: 1036 188 2077 977 1327
Fragments arranged by size:
2077 1327 1036 977 188
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 2600 3348 4920 2600
Size: 748 1572 3285
Fragments arranged by size:
3285 1572 748
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2220 2489 2914 3140 3292 5401 1493
Size: 727 269 425 226 152 2109 1697
Fragments arranged by size:
2109 1697 727 425 269 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 2183 2930 1870
Size: 73 191 49 747 4545
Fragments arranged by size:
4545 747 191 73 49
BamHI G'GATC_C
Cuts at: 2633 2770 2935 3302 2633
Size: 137 165 367 4936
Fragments arranged by size:
4936 367 165 137
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2142 2165 2926 4381 618
Size: 325 217 627 355 23 761 1455 1842
Fragments arranged by size:
1842 1455 761 627 355 325 217 23
BanII G_rGCy'C
Cuts at: 1391 2071 2924 1391
Size: 680 853 4072
Fragments arranged by size:
4072 853 680
BbeI G_GCGC'C
Cuts at: 2146 2146
Size: 5605
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 928
Size: 971 423 4211
Fragments arranged by size:
4211 971 423
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2102 3497 4707 2102
Size: 1395 1210 3000
Fragments arranged by size:
3000 1395 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2392 5600 2392
Size: 3208 2397
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2168 3666 4822 1790
Size: 378 1498 1156 2573
Fragments arranged by size:
2573 1498 1156 378
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2090 2090
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 2551 4265 1239
Size: 1220 92 1714 2579
Fragments arranged by size:
2579 1714 1220 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2180 4268 882
Size: 617 681 2088 2219
Fragments arranged by size:
2219 2088 681 617
BsaAI yAC'GTr
Cuts at: 493 1204 3396 493
Size: 711 2192 2702
Fragments arranged by size:
2702 2192 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2143 3412 3794
Size: 53 83 186 936 552 58 1269 382
Cuts at: 3794 4429 275
Size: 635 1451
Fragments arranged by size:
1451 1269 936 635 552 382 186 83
58 53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5605
BseYI C'CCAG_C
Cuts at: 4808 5304 4808
Size: 496 5109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5032
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 3666 3751 4822 2121
Size: 1545 85 1071 2904
Fragments arranged by size:
2904 1545 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2121 2168 2924 3666 3751 4822
Size: 399 281 50 47 756 742 85 1071
Cuts at: 4822 1391
Size: 2174
Fragments arranged by size:
2174 1071 756 742 399 281 85 50
47
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2577 2960 844
Size: 1733 383 3489
Fragments arranged by size:
3489 1733 383
BsrBI CCG'CTC
Cuts at: 1394 1823 2157 3492 1394
Size: 429 334 1335 3507
Fragments arranged by size:
3507 1335 429 334
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2052 4249 4436 1700
Size: 110 242 2197 187 2869
Fragments arranged by size:
2869 2197 242 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2600 794
Size: 1806 3799
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2392 2633 2770 2935 3302 3687 3704 5156
Size: 420 241 137 165 367 385 17 1452
Cuts at: 5156 5242 5254 5600 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 420 385 367 346 241 165
137 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2877 2877
Size: 5605
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2215 487
Size: 683 56 989 3877
Fragments arranged by size:
3877 989 683 56
BtsI GCAGTG_nn'
Cuts at: 2156 3121 3915 3935 2156
Size: 965 794 20 3826
Fragments arranged by size:
3826 965 794 20
ClaI AT'CG_AT
Cuts at: 1554 2795 1554
Size: 1241 4364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DraIII CAC_nnn'GTG
Cuts at: 2481 2481
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3052 3941
Size: 54 1425 50 275 18 177 1045 889
Cuts at: 3941 5570 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1045 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2737 3535 1353
Size: 1384 798 3423
Fragments arranged by size:
3423 1384 798
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2459 2551 2843 3668 4265 5052
Size: 393 699 128 92 292 825 597 787
Cuts at: 5052 5320 1239
Size: 268 1524
Fragments arranged by size:
1524 825 787 699 597 393 292 268
128 92
EcoRI G'AATT_C
Cuts at: 2489 2914 3292 2489
Size: 425 378 4802
Fragments arranged by size:
4802 425 378
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 2146 2179 4752 1678
Size: 468 33 2573 2531
Fragments arranged by size:
2573 2531 468 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2777 2809 2870 2902 4246
Size: 32 135 32 1205 32 61 32 1344
Cuts at: 4246 4278 4320 4352 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1344 1205 135 61 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KasI G'GCGC_C
Cuts at: 2142 2142
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4719 4903 1305
Size: 3414 184 2007
Fragments arranged by size:
3414 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3606
Fragments arranged by size:
3606 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3563 3922 4081 518
Size: 648 2397 359 159 2042
Fragments arranged by size:
2397 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2422 3021 3698 4846 5091 1606
Size: 816 599 677 1148 245 2120
Fragments arranged by size:
2120 1148 816 677 599 245
NaeI GCC'GGC
Cuts at: 2054 2054
Size: 5605
NarI GG'CG_CC
Cuts at: 2143 2143
Size: 5605
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NgoMIV G'CCGG_C
Cuts at: 2052 2052
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2243 2431 2963 4508 1132
Size: 97 1014 188 532 1545 2229
Fragments arranged by size:
2229 1545 1014 532 188 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 2239 2427 4504 2239
Size: 188 2077 3340
Fragments arranged by size:
3340 2077 188
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 2801 3175 2801
Size: 374 5231
PstI C_TGCA'G
Cuts at: 830 2546 2957 830
Size: 1716 411 3478
Fragments arranged by size:
3478 1716 411
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
RsrII CG'GwC_CG
Cuts at: 2159 2159
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2542 2839 2953 4088 4769 4960 826
Size: 219 1497 297 114 1135 681 191 1471
Fragments arranged by size:
1497 1471 1135 681 297 219 191 114
SfoI GGC'GCC
Cuts at: 2144 2144
Size: 5605
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 2815 3529 5
Size: 47 2763 714 2081
Fragments arranged by size:
2763 2081 714 47
StuI AGG'CCT
Cuts at: 1517 2723 1517
Size: 1206 4399
StyI C'CwwG_G
Cuts at: 513 1065 2004 3044 513
Size: 552 939 1040 3074
Fragments arranged by size:
3074 1040 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2716 3632 3649 3802 3987 4432 325
Size: 1255 1136 916 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1136 916 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 3851 5563
Size: 274 80 33 51 1443 234 1640 1712
Cuts at: 5563 96
Size: 138
Fragments arranged by size:
1712 1640 1443 274 234 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2078 2785 2078
Size: 707 4898
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI