Restriction map for
pFN21AE6227
(Circular) (Six-base) MAPSORT of: pFN21AE6227.seq Check: 5246 from: 1 to: 5362
pFN21AE6227 5362 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3172 278
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2445 2705 1937
Size: 508 260 4594
Fragments arranged by size:
4594 508 260
Acc65I G'GTAC_C
Cuts at: 2093 2683 2093
Size: 590 4772
AclI AA'CG_TT
Cuts at: 1563 3489 3862 1563
Size: 1926 373 3063
Fragments arranged by size:
3063 1926 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2332 3425 4809 5077 1632
Size: 700 1093 1384 268 1917
Fragments arranged by size:
1917 1384 1093 700 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5165
AflIII A'CryG_T
Cuts at: 1203 4261 5238 1203
Size: 3058 977 1327
Fragments arranged by size:
3058 1327 977
AgeI A'CCGG_T
Cuts at: 4193 4193
Size: 5362
AhdI GACnn_n'nnGTC
Cuts at: 4091 4091
Size: 5362
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2427 1184
Size: 1243 4119
AlwNI CAG_nnn'CTG
Cuts at: 3105 4677 3105
Size: 1572 3790
ApaLI G'TGCA_C
Cuts at: 3419 4575 3419
Size: 1156 4206
ApoI r'AATT_y
Cuts at: 1493 2671 2897 3049 5158 1493
Size: 1178 226 152 2109 1697
Fragments arranged by size:
2109 1697 1178 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3916 160
Size: 872 2884 1606
Fragments arranged by size:
2884 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
AvaI C'yCGr_G
Cuts at: 1870 1943 2687 1870
Size: 73 744 4545
Fragments arranged by size:
4545 744 73
BamHI G'GATC_C
Cuts at: 2692 3059 2692
Size: 367 4995
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2683 4138 618
Size: 325 217 627 306 590 1455 1842
Fragments arranged by size:
1842 1455 627 590 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2235 2681 1391
Size: 844 446 4072
Fragments arranged by size:
4072 844 446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4391
BbvCI CC'TCA_GC
Cuts at: 2555 2555
Size: 5362
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3553 3587 1903
Size: 34 1616 34 3678
Fragments arranged by size:
3678 1616 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3254 4464 3254
Size: 1210 4152
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5362
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 3973 136
Size: 107 122 71 631 605 358 1943 1525
Fragments arranged by size:
1943 1525 631 605 358 122 107 71
BglII A'GATC_T
Cuts at: 2040 2388 5357 2040
Size: 348 2969 2045
Fragments arranged by size:
2969 2045 348
BlpI GC'TnA_GC
Cuts at: 2779 2779
Size: 5362
Bme1580I G_kGCm'C
Cuts at: 1790 3423 4579 1790
Size: 1633 1156 2573
Fragments arranged by size:
2573 1633 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3093 4051 442
Size: 416 53 83 399 24 1676 958 1753
Fragments arranged by size:
1753 1676 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4022 1239
Size: 2783 2579
Bpu10I CC'TnA_GC
Cuts at: 2555 2555
Size: 5362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2449 3487 4352 4650 2449
Size: 1038 865 298 3161
Fragments arranged by size:
3161 1038 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4025 882
Size: 617 2526 2219
Fragments arranged by size:
2526 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3153 493
Size: 711 1949 2702
Fragments arranged by size:
2702 1949 711
BsaBI GATnn'nnATC
Cuts at: 5356 5356
Size: 5362
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2568 3169 3551 4186
Size: 53 83 186 936 1035 601 382 635
Cuts at: 4186 275
Size: 1451
Fragments arranged by size:
1451 1035 936 635 601 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3795 4193 4467 4614 5025 3795
Size: 398 274 147 411 4132
Fragments arranged by size:
4132 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2279 1178
Size: 30 165 30 846 30 4261
Fragments arranged by size:
4261 846 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2254 2612 2669 1186
Size: 1068 358 57 3879
Fragments arranged by size:
3879 1068 358 57
BseYI C'CCAG_C
Cuts at: 2512 2532 4565 5061 2512
Size: 20 2033 496 2813
Fragments arranged by size:
2813 2033 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2506 1163
Size: 573 770 4019
Fragments arranged by size:
4019 770 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2812 3573 4601 1540
Size: 275 186 811 761 1028 2301
Fragments arranged by size:
2301 1028 811 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2235 3423 3508 4579 2235
Size: 1188 85 1071 3018
Fragments arranged by size:
3018 1188 1071 85
BsmI GAATG_Cn'
Cuts at: 2873 2966 2873
Size: 93 5269
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2681 3423 3508 4579 1391
Size: 399 445 446 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 445 399 85
BspEI T'CCGG_A
Cuts at: 5025 5025
Size: 5362
BspHI T'CATG_A
Cuts at: 3251 4981 3251
Size: 1730 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2717 844
Size: 1873 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3249 1394
Size: 429 1426 3507
Fragments arranged by size:
3507 1426 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3857 4031 65
Size: 1183 2609 174 1396
Fragments arranged by size:
2609 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2607 4006 4193 1700
Size: 110 210 587 1399 187 2869
Fragments arranged by size:
2869 1399 587 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5362
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5362
BssSI C'ACGA_G
Cuts at: 3422 4434 3422
Size: 1012 4350
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5362
BstBI TT'CG_AA
Cuts at: 4166 4166
Size: 5362
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5362
BstYI r'GATC_y
Cuts at: 1972 2040 2388 2692 3059 3444 3461 4913 4999
Size: 68 348 304 367 385 17 1452 86
Cuts at: 4999 5011 5357 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 348 346 304 86
68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 513
Size: 552 939 72 3799
Fragments arranged by size:
3799 939 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4623
Fragments arranged by size:
4623 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 2878 3672 3692 2518
Size: 360 794 20 4188
Fragments arranged by size:
4188 794 360 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5362
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5327
DraI TTT'AAA
Cuts at: 2666 2666
Size: 5362
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4369 809
Size: 507 3053 1802
Fragments arranged by size:
3053 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 2415 2547
Size: 54 1425 50 275 18 543 42 132
Cuts at: 2547 2809 3698 5327 8
Size: 262 889 1629 43
Fragments arranged by size:
1629 1425 889 543 275 262 132 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2809 1537
Size: 275 997 4090
Fragments arranged by size:
4090 997 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2138 3292 1353
Size: 785 1154 3423
Fragments arranged by size:
3423 1154 785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3949 4323 4469 387
Size: 1064 138 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2233 2233
Size: 5362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2332 3425 4022 4809 5077 1239
Size: 393 700 1093 597 787 268 1524
Fragments arranged by size:
1524 1093 787 700 597 393 268
EcoRI G'AATT_C
Cuts at: 2671 3049 2671
Size: 378 4984
FspI TGC'GCA
Cuts at: 3868 3868
Size: 5362
HaeII r_GCGC'y
Cuts at: 1678 4509 1678
Size: 2831 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 4003 4035 4077
Size: 32 135 32 677 32 1722 32 42
Cuts at: 4077 4109 1373
Size: 32 2626
Fragments arranged by size:
2626 1722 677 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2167 2706 2952 669
Size: 988 281 229 539 246 3079
Fragments arranged by size:
3079 988 539 281 246 229
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5362
HpaI GTT'AAC
Cuts at: 2952 2952
Size: 5362
KpnI G_GTAC'C
Cuts at: 2097 2687 2097
Size: 590 4772
MfeI C'AATT_G
Cuts at: 2961 2961
Size: 5362
MluI A'CGCG_T
Cuts at: 5238 5238
Size: 5362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4476 4660 1305
Size: 3171 184 2007
Fragments arranged by size:
3171 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 10
Size: 54 1425 343 717 2823
Fragments arranged by size:
2823 1425 717 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3320 3679 3838 518
Size: 648 2154 359 159 2042
Fragments arranged by size:
2154 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2407 2532 2778 3455 4603 4848 1606
Size: 801 125 246 677 1148 245 2120
Fragments arranged by size:
2120 1148 801 677 246 245 125
NaeI GCC'GGC
Cuts at: 2022 2022
Size: 5362
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3871
Fragments arranged by size:
3871 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5362
NgoMIV G'CCGG_C
Cuts at: 2020 2020
Size: 5362
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5362
NotI GC'GGCC_GC
Cuts at: 2809 2809
Size: 5362
NruI TCG'CGA
Cuts at: 2071 2071
Size: 5362
NspI r_CATG'y
Cuts at: 1132 1229 2320 2485 2720 4265 1132
Size: 97 1091 165 235 1545 2229
Fragments arranged by size:
2229 1545 1091 235 165 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5362
PciI A'CATG_T
Cuts at: 4261 4261
Size: 5362
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2532 1108
Size: 1424 3938
PmeI GTTT'AAAC
Cuts at: 2666 2666
Size: 5362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 4944 4976 3431
Size: 32 1481 32 3817
Fragments arranged by size:
3817 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5362
PsiI TTA'TAA
Cuts at: 2932 2932
Size: 5362
PstI C_TGCA'G
Cuts at: 830 2714 830
Size: 1884 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5362
PvuII CAG'CTG
Cuts at: 2407 2407
Size: 5362
SacI G_AGCT'C
Cuts at: 2235 2235
Size: 5362
SalI G'TCGA_C
Cuts at: 1936 2704 1936
Size: 768 4594
SbfI CC_TGCA'GG
Cuts at: 2714 2714
Size: 5362
ScaI AGT'ACT
Cuts at: 3610 5322 3610
Size: 1712 3650
SfcI C'TryA_G
Cuts at: 826 1045 2710 3845 4526 4717 826
Size: 219 1665 1135 681 191 1471
Fragments arranged by size:
1665 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5362
SmaI CCC'GGG
Cuts at: 1872 2689 1872
Size: 817 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2464 3466 4367 4629 820
Size: 197 926 521 1002 901 262 1553
Fragments arranged by size:
1553 1002 926 901 521 262 197
SnaBI TAC'GTA
Cuts at: 493 3153 493
Size: 2660 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5362
SphI G_CATG'C
Cuts at: 1132 2720 1132
Size: 1588 3774
SspI AAT'ATT
Cuts at: 5 52 3286 5
Size: 47 3234 2081
Fragments arranged by size:
3234 2081 47
StuI AGG'CCT
Cuts at: 1517 2424 1517
Size: 907 4455
StyI C'CwwG_G
Cuts at: 513 1065 2004 2310 2801 513
Size: 552 939 306 491 3074
Fragments arranged by size:
3074 939 552 491 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3389 3406 3559 3744 4189 325
Size: 1255 1809 17 153 185 445 1498
Fragments arranged by size:
1809 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 3608 5320
Size: 274 80 33 51 1443 528 1103 1712
Cuts at: 5320 96
Size: 138
Fragments arranged by size:
1712 1443 1103 528 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5322 5354 5322
Size: 32 5330
Tth111I GACn'n_nGTC
Cuts at: 1316 2162 1316
Size: 846 4516
XbaI T'CTAG_A
Cuts at: 2276 2698 2276
Size: 422 4940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2302 2302
Size: 5362
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5362
XmaI C'CCGG_G
Cuts at: 1870 2687 1870
Size: 817 4545
XmnI GAAnn'nnTTC
Cuts at: 3491 3491
Size: 5362
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3170 276
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI