Restriction map for
pFN21AE6223
(Circular) (Six-base) MAPSORT of: pFN21AE6223.seq Check: 6519 from: 1 to: 5425
pFN21AE6223 5425 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2677 2677
Size: 5425
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2256 2746 2256
Size: 490 4935
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2669 3488 4872 5140 1632
Size: 1037 819 1384 268 1917
Fragments arranged by size:
1917 1384 1037 819 268
AflII C'TTAA_G
Cuts at: 820 1017 2125 820
Size: 197 1108 4120
Fragments arranged by size:
4120 1108 197
AflIII A'CryG_T
Cuts at: 1203 4324 5301 1203
Size: 3121 977 1327
Fragments arranged by size:
3121 1327 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 2032 4154 2032
Size: 2122 3303
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2295 1184
Size: 1111 4314
AlwNI CAG_nnn'CTG
Cuts at: 2269 2401 2695 3168 4740 2269
Size: 132 294 473 1572 2954
Fragments arranged by size:
2954 1572 473 294 132
ApaLI G'TGCA_C
Cuts at: 3482 4638 3482
Size: 1156 4269
ApoI r'AATT_y
Cuts at: 1493 2734 2960 3112 5221 1493
Size: 1241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2444 2750 1870
Size: 73 501 306 4545
Fragments arranged by size:
4545 501 306 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2164 2256 2746 4201 618
Size: 325 217 627 377 92 490 1455 1842
Fragments arranged by size:
1842 1455 627 490 377 325 217 92
BanII G_rGCy'C
Cuts at: 1391 2744 1391
Size: 1353 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2548 928
Size: 971 649 3805
Fragments arranged by size:
3805 971 649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2120 2154 3616 3650 1903
Size: 34 183 34 1462 34 3678
Fragments arranged by size:
3678 1462 183 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BfrBI ATG'CAT
Cuts at: 2530 2530
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2414 4036 136
Size: 107 122 71 631 605 742 1622 1525
Fragments arranged by size:
1622 1525 742 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5420 5420
Size: 5425
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2284 3486 4642 1790
Size: 353 141 1202 1156 2573
Fragments arranged by size:
2573 1202 1156 353 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 4114 442
Size: 416 53 83 399 24 1739 958 1753
Fragments arranged by size:
1753 1739 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2444 4085 1239
Size: 794 411 1641 2579
Fragments arranged by size:
2579 1641 794 411
Bpu10I CC'TnA_GC
Cuts at: 2497 2497
Size: 5425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 3550 4415 4713 2141
Size: 1409 865 298 2853
Fragments arranged by size:
2853 1409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4088 882
Size: 617 2589 2219
Fragments arranged by size:
2589 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3216 493
Size: 711 2012 2702
Fragments arranged by size:
2702 2012 711
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3232 3614 4249 275
Size: 53 83 186 936 1699 382 635 1451
Fragments arranged by size:
1699 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3858 4256 4530 4677 5088 3858
Size: 398 274 147 411 4195
Fragments arranged by size:
4195 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5200
Fragments arranged by size:
5200 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5425
BseYI C'CCAG_C
Cuts at: 2037 4628 5124 2037
Size: 2591 496 2338
Fragments arranged by size:
2591 2338 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4852
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2875 3636 4664 1540
Size: 275 186 874 761 1028 2301
Fragments arranged by size:
2301 1028 874 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3486 3571 4642 3486
Size: 85 1071 4269
Fragments arranged by size:
4269 1071 85
BsmI GAATG_Cn'
Cuts at: 2936 3029 2936
Size: 93 5332
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2284 2744 3486 3571 4642 1391
Size: 399 353 141 460 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 460 399 353 141 85
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 3314 5044 3314
Size: 1730 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 2277 2677 2780 844
Size: 1193 240 400 103 3489
Fragments arranged by size:
3489 1193 400 240 103
BsrBI CCG'CTC
Cuts at: 1394 1823 3312 1394
Size: 429 1489 3507
Fragments arranged by size:
3507 1489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3920 4094 65
Size: 1183 2672 174 1396
Fragments arranged by size:
2672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4069 4256 1700
Size: 110 2259 187 2869
Fragments arranged by size:
2869 2259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5425
BssSI C'ACGA_G
Cuts at: 3485 4497 3485
Size: 1012 4413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5425
BstBI TT'CG_AA
Cuts at: 2559 4229 2559
Size: 1670 3755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2104 2755 3122 3507 3524 4976 5062 5074
Size: 132 651 367 385 17 1452 86 12
Cuts at: 5074 5420 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 651 385 367 346 132 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2262 2262
Size: 5425
BtgI C'CryG_G
Cuts at: 513 1065 2004 2535 513
Size: 552 939 531 3403
Fragments arranged by size:
3403 939 552 531
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2145 487
Size: 683 56 919 3767
Fragments arranged by size:
3767 919 683 56
BtsI GCAGTG_nn'
Cuts at: 2613 2941 3735 3755 2613
Size: 328 794 20 4283
Fragments arranged by size:
4283 794 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2577 2712
Size: 54 1425 50 275 18 378 369 135
Cuts at: 2712 2872 3761 5390 8
Size: 160 889 1629 43
Fragments arranged by size:
1629 1425 889 378 369 275 160 135
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 1537
Size: 275 1060 4090
Fragments arranged by size:
4090 1060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2627 3355 1353
Size: 1274 728 3423
Fragments arranged by size:
3423 1274 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4012 4386 4532 387
Size: 1064 138 2423 374 146 1280
Fragments arranged by size:
2423 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2444 2669 3488 4085 4872 5140
Size: 393 401 411 225 819 597 787 268
Cuts at: 5140 1239
Size: 1524
Fragments arranged by size:
1524 819 787 597 411 401 393 268
225
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 2624 2159
Size: 465 4960
EcoO109I rG'GnC_Cy
Cuts at: 2242 2266 2617 2242
Size: 24 351 5050
Fragments arranged by size:
5050 351 24
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FspI TGC'GCA
Cuts at: 2096 3931 2096
Size: 1835 3590
HaeII r_GCGC'y
Cuts at: 1678 4572 1678
Size: 2894 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2101 2133 2372
Size: 32 135 32 446 32 51 32 239
Cuts at: 2372 2404 4066 4098 4140 4172 1373
Size: 32 1662 32 42 32 2626
Fragments arranged by size:
2626 1662 446 239 135 51 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KpnI G_GTAC'C
Cuts at: 2260 2750 2260
Size: 490 4935
MfeI C'AATT_G
Cuts at: 3024 3024
Size: 5425
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2403 4539 4723 1305
Size: 1098 2136 184 2007
Fragments arranged by size:
2136 2007 1098 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2579 2714 10
Size: 54 1425 343 378 369 135 2721
Fragments arranged by size:
2721 1425 378 369 343 135 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3383 3742 3901 518
Size: 648 2217 359 159 2042
Fragments arranged by size:
2217 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2841 3518 4666 4911 1606
Size: 1235 677 1148 245 2120
Fragments arranged by size:
2120 1235 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3934
Fragments arranged by size:
3934 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NsiI A_TGCA'T
Cuts at: 2532 2532
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2783 4328 1132
Size: 97 1554 1545 2229
Fragments arranged by size:
2229 1554 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2703 1348
Size: 1355 4070
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 1108
Size: 1293 4132
PfoI T'CCnGG_A
Cuts at: 2625 2625
Size: 5425
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2470 3494 3526 5007 5039 2438
Size: 32 1024 32 1481 32 2824
Fragments arranged by size:
2824 1481 1024 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 2266 2617 2242
Size: 24 351 5050
Fragments arranged by size:
5050 351 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5425
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PspXI vC'TCGA_Gb
Cuts at: 2444 2444
Size: 5425
PstI C_TGCA'G
Cuts at: 830 2274 2439 2674 2777 830
Size: 1444 165 235 103 3478
Fragments arranged by size:
3478 1444 235 165 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SbfI CC_TGCA'GG
Cuts at: 2274 2777 2274
Size: 503 4922
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SfcI C'TryA_G
Cuts at: 826 1045 2270 2435 2670 2773 3908 4589 4780
Size: 219 1225 165 235 103 1135 681 191
Cuts at: 4780 826
Size: 1471
Fragments arranged by size:
1471 1225 1135 681 235 219 191 165
103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2752 1872
Size: 880 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2125 2156 2444 3529 4430 4692
Size: 197 926 182 31 288 1085 901 262
Cuts at: 4692 820
Size: 1553
Fragments arranged by size:
1553 1085 926 901 288 262 197 182
31
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 2471 1517
Size: 954 4471
StyI C'CwwG_G
Cuts at: 513 1065 2004 2655 2864 513
Size: 552 939 651 209 3074
Fragments arranged by size:
3074 939 651 552 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2366 2528 2695 3452 3469 3622 3807
Size: 1255 786 162 167 757 17 153 185
Cuts at: 3807 4252 325
Size: 445 1498
Fragments arranged by size:
1498 1255 786 757 445 185 167 162
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3671 5383 96
Size: 274 80 33 51 1443 1694 1712 138
Fragments arranged by size:
1712 1694 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5385 5417 5385
Size: 32 5393
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5425
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 2444 1943
Size: 501 4924
XmaI C'CCGG_G
Cuts at: 1870 2750 1870
Size: 880 4545
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI