Restriction map for
pFN21AE6220
(Circular) (Six-base) MAPSORT of: pFN21AE6220.seq Check: 138 from: 1 to: 5569
pFN21AE6220 5569 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2583 2912 1937
Size: 646 329 4594
Fragments arranged by size:
4594 646 329
Acc65I G'GTAC_C
Cuts at: 2289 2890 2289
Size: 601 4968
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 3632 5016 5284 1632
Size: 455 1545 1384 268 1917
Fragments arranged by size:
1917 1545 1384 455 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 2247 2703 2780 3312 4884 2247
Size: 456 77 532 1572 2932
Fragments arranged by size:
2932 1572 532 456 77
ApaI G_GGCC'C
Cuts at: 2222 2320 2222
Size: 98 5471
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2450 2470 2878 3104 3256 5365 1493
Size: 957 20 408 226 152 2109 1697
Fragments arranged by size:
2109 1697 957 408 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2894 1870
Size: 73 951 4545
Fragments arranged by size:
4545 951 73
BamHI G'GATC_C
Cuts at: 2019 2899 3266 2019
Size: 880 367 4322
Fragments arranged by size:
4322 880 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2270 2289 2376 2723 2890
Size: 325 217 627 483 19 87 347 167
Cuts at: 2890 4345 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 483 347 325 217 167
87 19
BanII G_rGCy'C
Cuts at: 1391 2152 2179 2222 2320 2888 1391
Size: 761 27 43 98 568 4072
Fragments arranged by size:
4072 761 568 98 43 27
BbeI G_GCGC'C
Cuts at: 2274 2380 2274
Size: 106 5463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2075 2529 928
Size: 971 176 454 3968
Fragments arranged by size:
3968 971 454 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3760 3794 1903
Size: 34 1823 34 3678
Fragments arranged by size:
3678 1823 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5569
BfrBI ATG'CAT
Cuts at: 2738 2738
Size: 5569
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4180 136
Size: 107 122 71 631 605 2508 1525
Fragments arranged by size:
2508 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2986 2986
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1790 2222 2320 2624 3630 4786 1790
Size: 432 98 304 1006 1156 2573
Fragments arranged by size:
2573 1156 1006 432 304 98
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2130 2318 2509
Size: 416 53 83 399 24 713 188 191
Cuts at: 2509 2612 2821 3300 4258 442
Size: 103 209 479 958 1753
Fragments arranged by size:
1753 958 713 479 416 399 209 191
188 103 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 2474 4229 1239
Size: 897 338 1755 2579
Fragments arranged by size:
2579 1755 897 338
Bpu10I CC'TnA_GC
Cuts at: 2639 2639
Size: 5569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2172 2299 3694 4559 4857 2172
Size: 127 1395 865 298 2884
Fragments arranged by size:
2884 1395 865 298 127
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4232 882
Size: 617 2733 2219
Fragments arranged by size:
2733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3360 493
Size: 711 2156 2702
Fragments arranged by size:
2702 2156 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2271 2377 3376 3758
Size: 53 83 186 936 738 106 999 382
Cuts at: 3758 4393 275
Size: 635 1451
Fragments arranged by size:
1451 999 936 738 635 382 186 106
83 53
BsaWI w'CCGG_w
Cuts at: 4002 4400 4674 4821 5232 4002
Size: 398 274 147 411 4339
Fragments arranged by size:
4339 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5344
Fragments arranged by size:
5344 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2561 1186
Size: 1375 4194
BseYI C'CCAG_C
Cuts at: 2084 2458 4772 5268 2084
Size: 374 2314 496 2385
Fragments arranged by size:
2385 2314 496 374
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2727 1163
Size: 573 991 4005
Fragments arranged by size:
4005 991 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2152 3630 3715 4786 2152
Size: 1478 85 1071 2935
Fragments arranged by size:
2935 1478 1071 85
BsmI GAATG_Cn'
Cuts at: 3080 3173 3080
Size: 93 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2179 2222 2320 2624 2888 3630
Size: 399 362 27 43 98 304 264 742
Cuts at: 3630 3715 4786 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 362 304 264 98
85 43 27
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 3458 5188 3458
Size: 1730 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2417 2924 844
Size: 1573 507 3489
Fragments arranged by size:
3489 1573 507
BsrBI CCG'CTC
Cuts at: 1394 1823 2283 2406 3456 1394
Size: 429 460 123 1050 3507
Fragments arranged by size:
3507 1050 460 429 123
BsrDI GCAATG_nn'
Cuts at: 65 1248 4064 4238 65
Size: 1183 2816 174 1396
Fragments arranged by size:
2816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4213 4400 1700
Size: 110 2403 187 2869
Fragments arranged by size:
2869 2403 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 3629 4641 3629
Size: 1012 4557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 2534 1184
Size: 1350 4219
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2801 2107
Size: 694 4875
BstYI r'GATC_y
Cuts at: 1972 2008 2019 2899 3266 3651 3668 5120 5206
Size: 36 11 880 367 385 17 1452 86
Cuts at: 5206 5218 5564 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 880 385 367 346 86 36
17 12 11
Bsu36I CC'TnA_GG
Cuts at: 2307 2818 2307
Size: 511 5058
BtgI C'CryG_G
Cuts at: 513 1065 2004 2719 2824 513
Size: 552 939 715 105 3258
Fragments arranged by size:
3258 939 715 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4830
Fragments arranged by size:
4830 683 56
BtsI GCAGTG_nn'
Cuts at: 2622 2715 2781 3085 3879 3899 2622
Size: 93 66 304 794 20 4292
Fragments arranged by size:
4292 794 304 93 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3016 3905 5534
Size: 54 1425 50 275 18 1186 889 1629
Cuts at: 5534 8
Size: 43
Fragments arranged by size:
1629 1425 1186 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3499 1353
Size: 2146 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2555 4156 4530 4676 387
Size: 1064 138 966 1601 374 146 1280
Fragments arranged by size:
1601 1280 1064 966 374 146 138
EcoICRI GAG'CTC
Cuts at: 2150 2150
Size: 5569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2136 2474 3632 4229 5016 5284
Size: 393 455 49 338 1158 597 787 268
Cuts at: 5284 1239
Size: 1524
Fragments arranged by size:
1524 1158 787 597 455 393 338 268
49
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 2576 2845 2306
Size: 270 269 5030
Fragments arranged by size:
5030 270 269
EcoO109I rG'GnC_Cy
Cuts at: 2054 2096 2183 2219 2237 2316 2317 2658 2054
Size: 42 87 36 18 79 1 341 4965
Fragments arranged by size:
4965 341 87 79 42 36 18 1
EcoRI G'AATT_C
Cuts at: 2450 2878 3256 2450
Size: 428 378 4763
Fragments arranged by size:
4763 428 378
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 2274 2380 4716 1678
Size: 596 106 2336 2531
Fragments arranged by size:
2531 2336 596 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2742 2774 2801 2833 4210
Size: 32 135 32 1170 32 27 32 1377
Cuts at: 4210 4242 4284 4316 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1377 1170 135 42 32 32 32
32 32 32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3159 669
Size: 988 281 975 246 3079
Fragments arranged by size:
3079 988 975 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KasI G'GCGC_C
Cuts at: 2270 2376 2270
Size: 106 5463
KpnI G_GTAC'C
Cuts at: 2293 2894 2293
Size: 601 4968
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4683 4867 1305
Size: 3378 184 2007
Fragments arranged by size:
3378 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3747
Fragments arranged by size:
3747 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2799 3527 3886 4045 518
Size: 648 1633 728 359 159 2042
Fragments arranged by size:
2042 1633 728 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2596 2985 3662 4810 5055 1606
Size: 482 508 389 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 508 482 389 245
NarI GG'CG_CC
Cuts at: 2271 2377 2271
Size: 106 5463
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4078
Fragments arranged by size:
4078 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NsiI A_TGCA'T
Cuts at: 2740 2740
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2927 4472 1132
Size: 97 1698 1545 2229
Fragments arranged by size:
2229 1698 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2100 1348
Size: 752 4817
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5569
PfoI T'CCnGG_A
Cuts at: 2143 2152 2143
Size: 9 5560
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 2183 2096
Size: 87 5482
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PspOMI G'GGCC_C
Cuts at: 2218 2316 2218
Size: 98 5471
PstI C_TGCA'G
Cuts at: 830 2629 2921 830
Size: 1799 292 3478
Fragments arranged by size:
3478 1799 292
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2088 2596 2088
Size: 508 5061
SacI G_AGCT'C
Cuts at: 2152 2152
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2419 2625 2917 4052 4733 4924 826
Size: 219 1374 206 292 1135 681 191 1471
Fragments arranged by size:
1471 1374 1135 681 292 219 206 191
SfoI GGC'GCC
Cuts at: 2272 2378 2272
Size: 106 5463
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2187 2278 3673 4574 4836 820
Size: 197 926 244 91 1395 901 262 1553
Fragments arranged by size:
1553 1395 926 901 262 244 197 91
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 2004 2223 3008 513
Size: 552 939 219 785 3074
Fragments arranged by size:
3074 939 785 552 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3596 3613 3766 3951 4396 325
Size: 1255 2016 17 153 185 445 1498
Fragments arranged by size:
2016 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3815 5527 96
Size: 274 80 33 51 1443 1838 1712 138
Fragments arranged by size:
1838 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 5529 5561 2000
Size: 32 3497 32 2008
Fragments arranged by size:
3497 2008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI