Restriction map for
pFN21AE6219
(Circular) (Six-base) MAPSORT of: pFN21AE6219.seq Check: 7324 from: 1 to: 5251
pFN21AE6219 5251 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3061 278
Size: 53 83 186 936 1525 2468
Fragments arranged by size:
2468 1525 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2594 1937
Size: 657 4594
Acc65I G'GTAC_C
Cuts at: 2572 2572
Size: 5251
AclI AA'CG_TT
Cuts at: 1563 3378 3751 1563
Size: 1815 373 3063
Fragments arranged by size:
3063 1815 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2202 2250 2447 3314 4698 4966 1632
Size: 446 124 48 197 867 1384 268 1917
Fragments arranged by size:
1917 1384 867 446 268 197 124 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5054
AflIII A'CryG_T
Cuts at: 1203 4150 5127 1203
Size: 2947 977 1327
Fragments arranged by size:
2947 1327 977
AgeI A'CCGG_T
Cuts at: 4082 4082
Size: 5251
AhdI GACnn_n'nnGTC
Cuts at: 3980 3980
Size: 5251
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5251
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2222 1184
Size: 1038 4213
AlwNI CAG_nnn'CTG
Cuts at: 2056 2994 4566 2056
Size: 938 1572 2741
Fragments arranged by size:
2741 1572 938
ApaLI G'TGCA_C
Cuts at: 3308 4464 3308
Size: 1156 4095
ApoI r'AATT_y
Cuts at: 1493 2560 2786 2938 5047 1493
Size: 1067 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3805 160
Size: 872 2773 1606
Fragments arranged by size:
2773 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5251
AvaI C'yCGr_G
Cuts at: 1870 1943 2167 2576 1870
Size: 73 224 409 4545
Fragments arranged by size:
4545 409 224 73
BamHI G'GATC_C
Cuts at: 2581 2948 2581
Size: 367 4884
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2572 4027 618
Size: 325 217 627 785 1455 1842
Fragments arranged by size:
1842 1455 785 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2174 2196 2390 2570 1391
Size: 783 22 194 180 4072
Fragments arranged by size:
4072 783 194 180 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2273 928
Size: 971 374 3906
Fragments arranged by size:
3906 971 374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3442 3476 1903
Size: 34 1505 34 3678
Fragments arranged by size:
3678 1505 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3143 4353 3143
Size: 1210 4041
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5251
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 3862 136
Size: 107 122 71 631 605 367 1823 1525
Fragments arranged by size:
1823 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 5246 5246
Size: 5251
BlpI GC'TnA_GC
Cuts at: 2668 2668
Size: 5251
Bme1580I G_kGCm'C
Cuts at: 1790 3312 4468 1790
Size: 1522 1156 2573
Fragments arranged by size:
2573 1522 1156
BmgBI CAC'GTC
Cuts at: 1318 2476 1318
Size: 1158 4093
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2982 3940 442
Size: 416 53 83 399 24 1565 958 1753
Fragments arranged by size:
1753 1565 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2303 2506 3911 1239
Size: 875 189 203 1405 2579
Fragments arranged by size:
2579 1405 875 203 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2357 3376 4241 4539 2357
Size: 1019 865 298 3069
Fragments arranged by size:
3069 1019 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3914 882
Size: 617 2415 2219
Fragments arranged by size:
2415 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3042 493
Size: 711 1838 2702
Fragments arranged by size:
2702 1838 711
BsaBI GATnn'nnATC
Cuts at: 5245 5245
Size: 5251
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3058 3440 4075 275
Size: 53 83 186 936 1525 382 635 1451
Fragments arranged by size:
1525 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3684 4082 4356 4503 4914 3684
Size: 398 274 147 411 4021
Fragments arranged by size:
4021 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5026
Fragments arranged by size:
5026 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2117 2249 2516 1186
Size: 844 87 132 267 3921
Fragments arranged by size:
3921 844 267 132 87
BseYI C'CCAG_C
Cuts at: 2383 4454 4950 2383
Size: 2071 496 2684
Fragments arranged by size:
2684 2071 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 2207 2282 1163
Size: 573 456 15 75 4132
Fragments arranged by size:
4132 573 456 75 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2361 2701 3462 4490 1540
Size: 275 186 360 340 761 1028 2301
Fragments arranged by size:
2301 1028 761 360 340 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2196 3312 3397 4468 2148
Size: 48 1116 85 1071 2931
Fragments arranged by size:
2931 1116 1071 85 48
BsmI GAATG_Cn'
Cuts at: 2762 2855 2762
Size: 93 5158
BsmBI CGTCTCn'nnnn_
Cuts at: 2466 2466
Size: 5251
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2174 2196 2390 2570 3312 3397
Size: 399 358 26 22 194 180 742 85
Cuts at: 3397 4468 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 194 180 85
26 22
BspEI T'CCGG_A
Cuts at: 4914 4914
Size: 5251
BspHI T'CATG_A
Cuts at: 3140 4870 3140
Size: 1730 3521
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2422 2475 2606 844
Size: 1578 53 131 3489
Fragments arranged by size:
3489 1578 131 53
BsrBI CCG'CTC
Cuts at: 1394 1823 2461 3138 1394
Size: 429 638 677 3507
Fragments arranged by size:
3507 677 638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3746 3920 65
Size: 1183 2498 174 1396
Fragments arranged by size:
2498 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3895 4082 1700
Size: 110 2085 187 2869
Fragments arranged by size:
2869 2085 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5251
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5251
BssSI C'ACGA_G
Cuts at: 3311 4323 3311
Size: 1012 4239
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5251
BstBI TT'CG_AA
Cuts at: 4055 4055
Size: 5251
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5251
BstXI CCAn_nnnn'nTGG
Cuts at: 2092 2092
Size: 5251
BstYI r'GATC_y
Cuts at: 1972 2581 2948 3333 3350 4802 4888 4900 5246
Size: 609 367 385 17 1452 86 12 346
Cuts at: 5246 1972
Size: 1977
Fragments arranged by size:
1977 1452 609 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2414 2414
Size: 5251
BtgI C'CryG_G
Cuts at: 513 1065 2004 2085 513
Size: 552 939 81 3679
Fragments arranged by size:
3679 939 552 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4512
Fragments arranged by size:
4512 683 56
BtsI GCAGTG_nn'
Cuts at: 2146 2336 2767 3561 3581 2146
Size: 190 431 794 20 3816
Fragments arranged by size:
3816 794 431 190 20
ClaI AT'CG_AT
Cuts at: 1554 2220 1554
Size: 666 4585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5216
DraI TTT'AAA
Cuts at: 2555 2555
Size: 5251
DraIII CAC_nnn'GTG
Cuts at: 2401 2401
Size: 5251
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4258 809
Size: 507 2942 1802
Fragments arranged by size:
2942 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2358 2698
Size: 54 1425 50 275 18 252 276 340
Cuts at: 2698 3587 5216 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 340 276 275 252 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2358 2698 1537
Size: 275 546 340 4090
Fragments arranged by size:
4090 546 340 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2226 3181 1353
Size: 701 172 955 3423
Fragments arranged by size:
3423 955 701 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2156 3838 4212 4358 387
Size: 1064 138 567 1682 374 146 1280
Fragments arranged by size:
1682 1280 1064 567 374 146 138
EcoICRI GAG'CTC
Cuts at: 2194 2194
Size: 5251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2114 2202 2250 2303 2447 2506
Size: 393 446 36 88 48 53 144 59
Cuts at: 2506 3314 3911 4698 4966 1239
Size: 808 597 787 268 1524
Fragments arranged by size:
1524 808 787 597 446 393 268 144
88 59 53 48 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2429 2429
Size: 5251
EcoO109I rG'GnC_Cy
Cuts at: 2377 2464 2377
Size: 87 5164
EcoRI G'AATT_C
Cuts at: 2560 2938 2560
Size: 378 4873
FspI TGC'GCA
Cuts at: 2351 3757 2351
Size: 1406 3845
FspAI rTGC'GCAy
Cuts at: 2351 2351
Size: 5251
HaeII r_GCGC'y
Cuts at: 1678 4398 1678
Size: 2720 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2492 2524 2531 2563 3892
Size: 32 135 32 920 32 7 32 1329
Cuts at: 3892 3924 3966 3998 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 920 135 42 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2595 2841 669
Size: 988 281 657 246 3079
Fragments arranged by size:
3079 988 657 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5251
HpaI GTT'AAC
Cuts at: 2841 2841
Size: 5251
KpnI G_GTAC'C
Cuts at: 2576 2576
Size: 5251
MfeI C'AATT_G
Cuts at: 2850 2850
Size: 5251
MluI A'CGCG_T
Cuts at: 5127 5127
Size: 5251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4365 4549 1305
Size: 3060 184 2007
Fragments arranged by size:
3060 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3429
Fragments arranged by size:
3429 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 3209 3568 3727 518
Size: 648 1086 957 359 159 2042
Fragments arranged by size:
2042 1086 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2206 2667 3344 4492 4737 1606
Size: 552 48 461 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 461 245 48
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3760
Fragments arranged by size:
3760 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5251
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5251
NotI GC'GGCC_GC
Cuts at: 2698 2698
Size: 5251
NspI r_CATG'y
Cuts at: 1132 1229 2356 2609 4154 1132
Size: 97 1127 253 1545 2229
Fragments arranged by size:
2229 1545 1127 253 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5251
PciI A'CATG_T
Cuts at: 4150 4150
Size: 5251
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5251
PmeI GTTT'AAAC
Cuts at: 2555 2555
Size: 5251
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 4833 4865 3320
Size: 32 1481 32 3706
Fragments arranged by size:
3706 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2464 2464
Size: 5251
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5251
PsiI TTA'TAA
Cuts at: 2821 2821
Size: 5251
PstI C_TGCA'G
Cuts at: 830 2076 2603 830
Size: 1246 527 3478
Fragments arranged by size:
3478 1246 527
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5251
PvuII CAG'CTG
Cuts at: 2206 2206
Size: 5251
SacI G_AGCT'C
Cuts at: 2196 2196
Size: 5251
SalI G'TCGA_C
Cuts at: 1936 2593 1936
Size: 657 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 5251
SbfI CC_TGCA'GG
Cuts at: 2603 2603
Size: 5251
ScaI AGT'ACT
Cuts at: 3499 5211 3499
Size: 1712 3539
SfcI C'TryA_G
Cuts at: 826 1045 2072 2599 3734 4415 4606 826
Size: 219 1027 527 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 527 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2039 2039
Size: 5251
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5251
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5251
SmaI CCC'GGG
Cuts at: 1872 2169 2578 1872
Size: 297 409 4545
Fragments arranged by size:
4545 409 297
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2372 3355 4256 4518 820
Size: 197 926 429 983 901 262 1553
Fragments arranged by size:
1553 983 926 901 429 262 197
SnaBI TAC'GTA
Cuts at: 493 3042 493
Size: 2549 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5251
SphI G_CATG'C
Cuts at: 1132 2609 1132
Size: 1477 3774
SspI AAT'ATT
Cuts at: 5 52 3175 5
Size: 47 3123 2081
Fragments arranged by size:
3123 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5251
StyI C'CwwG_G
Cuts at: 513 1065 2004 2690 513
Size: 552 939 686 3074
Fragments arranged by size:
3074 939 686 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 3278 3295 3448 3633 4078 325
Size: 1255 888 810 17 153 185 445 1498
Fragments arranged by size:
1498 1255 888 810 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3497 5209 96
Size: 274 80 33 51 1443 1520 1712 138
Fragments arranged by size:
1712 1520 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5211 5243 5211
Size: 32 5219
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5251
XbaI T'CTAG_A
Cuts at: 2587 2587
Size: 5251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2278
Size: 5251
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5251
XmaI C'CCGG_G
Cuts at: 1870 2167 2576 1870
Size: 297 409 4545
Fragments arranged by size:
4545 409 297
XmnI GAAnn'nnTTC
Cuts at: 3380 3380
Size: 5251
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3059 276
Size: 53 83 186 936 1525 2468
Fragments arranged by size:
2468 1525 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoRV FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfoI SrfI SwaI