Restriction map for
pFN21AE6217
(Circular) (Six-base) MAPSORT of: pFN21AE6217.seq Check: 6424 from: 1 to: 5497
pFN21AE6217 5497 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2361 3307 278
Size: 53 83 186 936 825 946 2468
Fragments arranged by size:
2468 946 936 825 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2391 2840 1937
Size: 454 449 4594
Fragments arranged by size:
4594 454 449
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3560 4944 5212 1632
Size: 1928 1384 268 1917
Fragments arranged by size:
1928 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 2520 4396 5373 1203
Size: 1317 1876 977 1327
Fragments arranged by size:
1876 1327 1317 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AlwNI CAG_nnn'CTG
Cuts at: 2718 3240 4812 2718
Size: 522 1572 3403
Fragments arranged by size:
3403 1572 522
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2699 2806 3032 3184 5293 1493
Size: 1206 107 226 152 2109 1697
Fragments arranged by size:
2109 1697 1206 226 152 107
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2822 1870
Size: 73 879 4545
Fragments arranged by size:
4545 879 73
BamHI G'GATC_C
Cuts at: 2424 2562 2827 3194 2424
Size: 138 265 367 4727
Fragments arranged by size:
4727 367 265 138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2818 4273 618
Size: 325 217 627 1031 1455 1842
Fragments arranged by size:
1842 1455 1031 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2816 1391
Size: 1425 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2441 2524 928
Size: 971 542 83 3901
Fragments arranged by size:
3901 971 542 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2276 3389 4599 2276
Size: 1113 1210 3174
Fragments arranged by size:
3174 1210 1113
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2107 4108
Size: 107 122 71 631 605 337 98 2001
Cuts at: 4108 136
Size: 1525
Fragments arranged by size:
2001 1525 631 605 337 122 107 98
71
BglII A'GATC_T
Cuts at: 2511 5492 2511
Size: 2981 2516
BlpI GC'TnA_GC
Cuts at: 2914 2914
Size: 5497
Bme1580I G_kGCm'C
Cuts at: 1790 3558 4714 1790
Size: 1768 1156 2573
Fragments arranged by size:
2573 1768 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2085 2282 2726
Size: 416 53 83 399 24 668 197 444
Cuts at: 2726 3228 4186 442
Size: 502 958 1753
Fragments arranged by size:
1753 958 668 502 444 416 399 197
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4157 1239
Size: 2918 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2744 3622 4487 4785 2744
Size: 878 865 298 3456
Fragments arranged by size:
3456 878 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4160 882
Size: 617 2661 2219
Fragments arranged by size:
2661 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2280 2358 2456 3304
Size: 53 83 186 936 747 78 98 848
Cuts at: 3304 3686 4321 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 848 747 635 382 186 98
83 78 53
BsaWI w'CCGG_w
Cuts at: 2598 3930 4328 4602 4749 5160 2598
Size: 1332 398 274 147 411 2935
Fragments arranged by size:
2935 1332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2181 2211 1178
Size: 30 165 30 778 30 4464
Fragments arranged by size:
4464 778 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5497
BseYI C'CCAG_C
Cuts at: 4700 5196 4700
Size: 496 5001
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4924
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3708 4736 1540
Size: 275 186 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3558 3643 4714 3558
Size: 85 1071 4341
Fragments arranged by size:
4341 1071 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2816 3558 3643 4714 1391
Size: 399 1026 742 85 1071 2174
Fragments arranged by size:
2174 1071 1026 742 399 85
BspEI T'CCGG_A
Cuts at: 2598 5160 2598
Size: 2562 2935
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2852 844
Size: 2008 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 2671 4141 4328 1700
Size: 110 291 570 1470 187 2869
Fragments arranged by size:
2869 1470 570 291 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstXI CCAn_nnnn'nTGG
Cuts at: 2276 2276
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2043 2424 2511 2562 2827 3194 3579 3596
Size: 71 381 87 51 265 367 385 17
Cuts at: 3596 5048 5134 5146 5492 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 381 367 346 265 87
86 71 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 2303 2303
Size: 5497
BtgI C'CryG_G
Cuts at: 513 1065 2004 2150 513
Size: 552 939 146 3860
Fragments arranged by size:
3860 939 552 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 3013 3807 3827 2358
Size: 655 794 20 4028
Fragments arranged by size:
4028 794 655 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2944 3833 5462
Size: 54 1425 50 275 18 1114 889 1629
Cuts at: 5462 8
Size: 43
Fragments arranged by size:
1629 1425 1114 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3560 4157 4944 5212 1239
Size: 393 1928 597 787 268 1524
Fragments arranged by size:
1928 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2052 2052
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2063 2121 4644 1678
Size: 385 58 2523 2531
Fragments arranged by size:
2531 2523 385 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4138 4170 4212 4244 1373
Size: 32 135 32 2566 32 42 32 2626
Fragments arranged by size:
2626 2566 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2341 2841 3087 669
Size: 988 281 403 500 246 3079
Fragments arranged by size:
3079 988 500 403 281 246
HindIII A'AGCT_T
Cuts at: 748 2762 748
Size: 2014 3483
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2248 4611 4795 1305
Size: 943 2363 184 2007
Fragments arranged by size:
2363 2007 943 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3675
Fragments arranged by size:
3675 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 2288 2316 3455 3814 3973 518
Size: 648 1108 14 28 1139 359 159 2042
Fragments arranged by size:
2042 1139 1108 648 359 159 28 14
MspA1I CmG'CkG
Cuts at: 1606 2913 3590 4738 4983 1606
Size: 1307 677 1148 245 2120
Fragments arranged by size:
2120 1307 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4006
Fragments arranged by size:
4006 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2524 2855 4400 1132
Size: 97 1295 331 1545 2229
Fragments arranged by size:
2229 1545 1295 331 97
PasI CC'CwG_GG
Cuts at: 1348 2718 1348
Size: 1370 4127
PciI A'CATG_T
Cuts at: 2520 4396 2520
Size: 1876 3621
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2031 1108
Size: 923 4574
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2849 830
Size: 2019 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2845 3980 4661 4852 826
Size: 219 1800 1135 681 191 1471
Fragments arranged by size:
1800 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2759 3601 4502 4764 820
Size: 197 926 816 842 901 262 1553
Fragments arranged by size:
1553 926 901 842 816 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2004 2793 2936 513
Size: 552 939 789 143 3074
Fragments arranged by size:
3074 939 789 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3524 3541 3694 3879 4324 325
Size: 1255 1944 17 153 185 445 1498
Fragments arranged by size:
1944 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2181 2213 5457 5489 2181
Size: 32 3244 32 2189
Fragments arranged by size:
3244 2189 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2359 3305 276
Size: 53 83 186 936 825 946 2468
Fragments arranged by size:
2468 946 936 825 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI