Restriction map for
pFN21AE6212
(Circular) (Six-base) MAPSORT of: pFN21AE6212.seq Check: 1513 from: 1 to: 5419
pFN21AE6212 5419 bp
With 182 enzymes: SgfI *
March 18, 2015 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3229 278
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2762 1937
Size: 825 4594
Acc65I G'GTAC_C
Cuts at: 2133 2740 2133
Size: 607 4812
AclI AA'CG_TT
Cuts at: 1563 3546 3919 1563
Size: 1983 373 3063
Fragments arranged by size:
3063 1983 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3482 4866 5134 1632
Size: 1850 1384 268 1917
Fragments arranged by size:
1917 1850 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5222
AflIII A'CryG_T
Cuts at: 1203 2127 2145 4318 5295 1203
Size: 924 18 2173 977 1327
Fragments arranged by size:
2173 1327 977 924 18
AgeI A'CCGG_T
Cuts at: 4250 4250
Size: 5419
AhdI GACnn_n'nnGTC
Cuts at: 4148 4148
Size: 5419
AleI CACnn'nnGTG
Cuts at: 1166 2561 1166
Size: 1395 4024
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2526 1184
Size: 1342 4077
AlwNI CAG_nnn'CTG
Cuts at: 3162 4734 3162
Size: 1572 3847
ApaLI G'TGCA_C
Cuts at: 2303 2698 3476 4632 2303
Size: 395 778 1156 3090
Fragments arranged by size:
3090 1156 778 395
ApoI r'AATT_y
Cuts at: 1493 2543 2728 2954 3106 5215 1493
Size: 1050 185 226 152 2109 1697
Fragments arranged by size:
2109 1697 1050 226 185 152
AseI AT'TA_AT
Cuts at: 160 1032 3973 160
Size: 872 2941 1606
Fragments arranged by size:
2941 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 2369 2744 1870
Size: 73 288 138 375 4545
Fragments arranged by size:
4545 375 288 138 73
BamHI G'GATC_C
Cuts at: 2749 3116 2749
Size: 367 5052
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2133 2740 4195 618
Size: 325 217 627 346 607 1455 1842
Fragments arranged by size:
1842 1455 627 607 346 325 217
BanII G_rGCy'C
Cuts at: 1391 2232 2378 2386 2420 2738 1391
Size: 841 146 8 34 318 4072
Fragments arranged by size:
4072 841 318 146 34 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2383 928
Size: 971 484 3964
Fragments arranged by size:
3964 971 484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2014 2048 3610 3644 1903
Size: 34 77 34 1562 34 3678
Fragments arranged by size:
3678 1562 77 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3311 4521 3311
Size: 1210 4209
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5419
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4030 136
Size: 107 122 71 631 605 2358 1525
Fragments arranged by size:
2358 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2100 5414 2100
Size: 3314 2105
BlpI GC'TnA_GC
Cuts at: 2836 2836
Size: 5419
Bme1580I G_kGCm'C
Cuts at: 1790 2307 2702 3480 4636 1790
Size: 517 395 778 1156 2573
Fragments arranged by size:
2573 1156 778 517 395
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3150 4108 442
Size: 416 53 83 399 24 1733 958 1753
Fragments arranged by size:
1753 1733 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2261 2570 4079 1239
Size: 842 180 309 1509 2579
Fragments arranged by size:
2579 1509 842 309 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3544 4409 4707 3544
Size: 865 298 4256
Fragments arranged by size:
4256 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4082 882
Size: 617 2583 2219
Fragments arranged by size:
2583 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2128 2173 3210 493
Size: 711 924 45 1037 2702
Fragments arranged by size:
2702 1037 924 711 45
BsaBI GATnn'nnATC
Cuts at: 5413 5413
Size: 5419
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2646 3226 3608
Size: 53 83 186 936 531 582 580 382
Cuts at: 3608 4243 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 582 580 531 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2477 3852 4250 4524 4671 5082 2477
Size: 1375 398 274 147 411 2814
Fragments arranged by size:
2814 1375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5194
Fragments arranged by size:
5194 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5419
BseYI C'CCAG_C
Cuts at: 2043 2152 2238 4622 5118 2043
Size: 109 86 2384 496 2344
Fragments arranged by size:
2384 2344 496 109 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2585 2594 1163
Size: 573 849 9 3988
Fragments arranged by size:
3988 849 573 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2438 2869 3630 4658 1540
Size: 275 186 437 431 761 1028 2301
Fragments arranged by size:
2301 1028 761 437 431 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2420 2702 3480 3565 4636 2307
Size: 113 282 778 85 1071 3090
Fragments arranged by size:
3090 1071 778 282 113 85
BsmI GAATG_Cn'
Cuts at: 2930 3023 2930
Size: 93 5326
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2307 2378 2386 2420 2702 2738
Size: 399 442 75 71 8 34 282 36
Cuts at: 2738 3480 3565 4636 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 442 399 282 85 75
71 36 34 8
BspEI T'CCGG_A
Cuts at: 2477 5082 2477
Size: 2605 2814
BspHI T'CATG_A
Cuts at: 3308 5038 3308
Size: 1730 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2280 2774 844
Size: 1436 494 3489
Fragments arranged by size:
3489 1436 494
BsrBI CCG'CTC
Cuts at: 1394 1823 2314 2622 3306 1394
Size: 429 491 308 684 3507
Fragments arranged by size:
3507 684 491 429 308
BsrDI GCAATG_nn'
Cuts at: 65 1248 2015 2667 3914 4088 65
Size: 1183 767 652 1247 174 1396
Fragments arranged by size:
1396 1247 1183 767 652 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4063 4250 1700
Size: 110 2253 187 2869
Fragments arranged by size:
2869 2253 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5419
BssHII G'CGCG_C
Cuts at: 1925 2243 1925
Size: 318 5101
BssSI C'ACGA_G
Cuts at: 2291 3479 4491 2291
Size: 1188 1012 3219
Fragments arranged by size:
3219 1188 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 2572 794
Size: 1427 351 3641
Fragments arranged by size:
3641 1427 351
BstBI TT'CG_AA
Cuts at: 2397 4223 2397
Size: 1826 3593
BstEII G'GTnAC_C
Cuts at: 1184 2470 2485 1184
Size: 1286 15 4118
Fragments arranged by size:
4118 1286 15
BstYI r'GATC_y
Cuts at: 1972 2100 2749 3116 3501 3518 4970 5056 5068
Size: 128 649 367 385 17 1452 86 12
Cuts at: 5068 5414 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 649 385 367 346 128 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2284 2589 513
Size: 552 1219 305 3343
Fragments arranged by size:
3343 1219 552 305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2728 487
Size: 683 56 1502 3178
Fragments arranged by size:
3178 1502 683 56
BtsI GCAGTG_nn'
Cuts at: 2935 3729 3749 2935
Size: 794 20 4605
Fragments arranged by size:
4605 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5384
DraI TTT'AAA
Cuts at: 2723 2723
Size: 5419
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2468 4426 809
Size: 507 1152 1958 1802
Fragments arranged by size:
1958 1802 1152 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2435 2866 3755
Size: 54 1425 50 275 18 605 431 889
Cuts at: 3755 5384 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 605 431 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2435 2866 1537
Size: 275 623 431 4090
Fragments arranged by size:
4090 623 431 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3349 1353
Size: 1996 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4006 4380 4526 387
Size: 1064 138 2417 374 146 1280
Fragments arranged by size:
2417 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2418 2418
Size: 5419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2261 2570 3482 4079 4866 5134
Size: 393 449 180 309 912 597 787 268
Cuts at: 5134 1239
Size: 1524
Fragments arranged by size:
1524 912 787 597 449 393 309 268
180
EcoRI G'AATT_C
Cuts at: 2543 2728 3106 2543
Size: 185 378 4856
Fragments arranged by size:
4856 378 185
FspI TGC'GCA
Cuts at: 3925 3925
Size: 5419
HaeII r_GCGC'y
Cuts at: 1678 4566 1678
Size: 2888 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2373 2405 4060 4092 4134
Size: 32 135 32 801 32 1655 32 42
Cuts at: 4134 4166 1373
Size: 32 2626
Fragments arranged by size:
2626 1655 801 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2763 3009 669
Size: 988 281 72 753 246 3079
Fragments arranged by size:
3079 988 753 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5419
HpaI GTT'AAC
Cuts at: 3009 3009
Size: 5419
KpnI G_GTAC'C
Cuts at: 2137 2744 2137
Size: 607 4812
MfeI C'AATT_G
Cuts at: 3018 3018
Size: 5419
MluI A'CGCG_T
Cuts at: 2145 5295 2145
Size: 3150 2269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4533 4717 1305
Size: 3228 184 2007
Fragments arranged by size:
3228 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3597
Fragments arranged by size:
3597 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2561 3377 3736 3895 518
Size: 648 1395 816 359 159 2042
Fragments arranged by size:
2042 1395 816 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2343 2591 2835 3512 4660 4905 1606
Size: 606 131 248 244 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 606 248 245 244 131
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4867
NdeI CA'TA_TG
Cuts at: 387 2091 387
Size: 1704 3715
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5419
NotI GC'GGCC_GC
Cuts at: 2866 2866
Size: 5419
NspI r_CATG'y
Cuts at: 1132 1229 2777 4322 1132
Size: 97 1548 1545 2229
Fragments arranged by size:
2229 1548 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5419
PciI A'CATG_T
Cuts at: 4318 4318
Size: 5419
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5419
PfoI T'CCnGG_A
Cuts at: 2547 2547
Size: 5419
PmeI GTTT'AAAC
Cuts at: 2723 2723
Size: 5419
PmlI CAC'GTG
Cuts at: 2128 2173 2128
Size: 45 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 5001 5033 3488
Size: 32 1481 32 3874
Fragments arranged by size:
3874 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5419
PsiI TTA'TAA
Cuts at: 2989 2989
Size: 5419
PstI C_TGCA'G
Cuts at: 830 2217 2265 2502 2771 830
Size: 1387 48 237 269 3478
Fragments arranged by size:
3478 1387 269 237 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5419
PvuII CAG'CTG
Cuts at: 2212 2212
Size: 5419
SacI G_AGCT'C
Cuts at: 2420 2420
Size: 5419
SacII CC_GC'GG
Cuts at: 2592 2592
Size: 5419
SalI G'TCGA_C
Cuts at: 1936 2761 1936
Size: 825 4594
SbfI CC_TGCA'GG
Cuts at: 2771 2771
Size: 5419
ScaI AGT'ACT
Cuts at: 3667 5379 3667
Size: 1712 3707
SfcI C'TryA_G
Cuts at: 826 1045 2213 2261 2498 2682 2767 3902 4583
Size: 219 1168 48 237 184 85 1135 681
Cuts at: 4583 4774 826
Size: 191 1471
Fragments arranged by size:
1471 1168 1135 681 237 219 191 184
85 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5419
SmaI CCC'GGG
Cuts at: 1872 2746 1872
Size: 874 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3523 4424 4686 820
Size: 197 926 1580 901 262 1553
Fragments arranged by size:
1580 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3210 493
Size: 2717 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5419
SphI G_CATG'C
Cuts at: 1132 2777 1132
Size: 1645 3774
SspI AAT'ATT
Cuts at: 5 52 3343 5
Size: 47 3291 2081
Fragments arranged by size:
3291 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5419
StyI C'CwwG_G
Cuts at: 513 1065 2319 2858 513
Size: 552 1254 539 3074
Fragments arranged by size:
3074 1254 552 539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2480 3446 3463 3616 3801 4246 325
Size: 1255 900 966 17 153 185 445 1498
Fragments arranged by size:
1498 1255 966 900 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3665 5377 96
Size: 274 80 33 51 1443 1688 1712 138
Fragments arranged by size:
1712 1688 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5379 5411 5379
Size: 32 5387
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5419
XbaI T'CTAG_A
Cuts at: 2378 2755 2378
Size: 377 5042
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5419
XmaI C'CCGG_G
Cuts at: 1870 2744 1870
Size: 874 4545
XmnI GAAnn'nnTTC
Cuts at: 3548 3548
Size: 5419
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3227 276
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI