Restriction map for
pFN21AE6204
(Circular) (Six-base) MAPSORT of: pFN21AE6204.seq Check: 3276 from: 1 to: 5527
pFN21AE6204 5527 bp
With 182 enzymes: SgfI *
March 18, 2015 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3337 278
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2848 2848
Size: 5527
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 3590 4974 5242 1632
Size: 527 1431 1384 268 1917
Fragments arranged by size:
1917 1431 1384 527 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 4426 5403 1203
Size: 3223 977 1327
Fragments arranged by size:
3223 1327 977
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2583 1184
Size: 1399 4128
AlwNI CAG_nnn'CTG
Cuts at: 3270 4842 3270
Size: 1572 3955
ApaI G_GGCC'C
Cuts at: 2033 2033
Size: 5527
ApaLI G'TGCA_C
Cuts at: 3584 4740 3584
Size: 1156 4371
ApoI r'AATT_y
Cuts at: 1493 2836 3062 3214 5323 1493
Size: 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2852 1870
Size: 73 83 826 4545
Fragments arranged by size:
4545 826 83 73
BamHI G'GATC_C
Cuts at: 2857 3224 2857
Size: 367 5160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 2257 2848 4303 618
Size: 325 217 627 317 153 591 1455 1842
Fragments arranged by size:
1842 1455 627 591 325 317 217 153
BanII G_rGCy'C
Cuts at: 1391 2033 2846 1391
Size: 642 813 4072
Fragments arranged by size:
4072 813 642
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 928
Size: 971 402 4154
Fragments arranged by size:
4154 971 402
BbvCI CC'TCA_GC
Cuts at: 2153 2153
Size: 5527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2007 2041 3718 3752 1903
Size: 34 70 34 1677 34 3678
Fragments arranged by size:
3678 1677 70 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3419 4629 3419
Size: 1210 4317
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5527
BfrBI ATG'CAT
Cuts at: 2738 2738
Size: 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4138 136
Size: 107 122 71 631 605 2466 1525
Fragments arranged by size:
2466 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5522 5522
Size: 5527
BlpI GC'TnA_GC
Cuts at: 2168 2944 2168
Size: 776 4751
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2109 3588 4744 1790
Size: 243 76 1479 1156 2573
Fragments arranged by size:
2573 1479 1156 243 76
BmgBI CAC'GTC
Cuts at: 1318 2058 1318
Size: 740 4787
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2401 3258 4216
Size: 416 53 83 399 24 984 857 958
Cuts at: 4216 442
Size: 1753
Fragments arranged by size:
1753 984 958 857 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2379 4187 1239
Size: 1140 1808 2579
Fragments arranged by size:
2579 1808 1140
Bpu10I CC'TnA_GC
Cuts at: 2153 2153
Size: 5527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3652 4517 4815 3652
Size: 865 298 4364
Fragments arranged by size:
4364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2790 3318 493
Size: 711 1586 528 2702
Fragments arranged by size:
2702 1586 711 528
BsaBI GATnn'nnATC
Cuts at: 2610 5521 2610
Size: 2911 2616
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3334 3716 4351 275
Size: 53 83 186 936 1801 382 635 1451
Fragments arranged by size:
1801 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3960 4358 4632 4779 5190 3960
Size: 398 274 147 411 4297
Fragments arranged by size:
4297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5302
Fragments arranged by size:
5302 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2757 1186
Size: 1571 3956
BseYI C'CCAG_C
Cuts at: 4730 5226 4730
Size: 496 5031
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 1163
Size: 573 378 4576
Fragments arranged by size:
4576 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2977 3738 4766 1540
Size: 275 186 976 761 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3588 3673 4744 3588
Size: 85 1071 4371
Fragments arranged by size:
4371 1071 85
BsmI GAATG_Cn'
Cuts at: 2401 3038 3131 2401
Size: 637 93 4797
Fragments arranged by size:
4797 637 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2109 2846 3588 3673 4744 1391
Size: 399 243 76 737 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 737 399 243 85 76
BspEI T'CCGG_A
Cuts at: 5190 5190
Size: 5527
BspHI T'CATG_A
Cuts at: 3416 5146 3416
Size: 1730 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2882 844
Size: 2038 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 2172 3414 1394
Size: 429 202 147 1242 3507
Fragments arranged by size:
3507 1242 429 202 147
BsrDI GCAATG_nn'
Cuts at: 65 1248 2676 4022 4196 65
Size: 1183 1428 1346 174 1396
Fragments arranged by size:
1428 1396 1346 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2187 4171 4358 1700
Size: 110 377 1984 187 2869
Fragments arranged by size:
2869 1984 377 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5527
BssHII G'CGCG_C
Cuts at: 1925 2076 1925
Size: 151 5376
BssSI C'ACGA_G
Cuts at: 3587 4599 3587
Size: 1012 4515
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2387 794
Size: 1593 3934
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2857 3224 3609 3626 5078 5164 5176 5522
Size: 885 367 385 17 1452 86 12 346
Cuts at: 5522 1972
Size: 1977
Fragments arranged by size:
1977 1452 885 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2062 2100 2205 2719 513
Size: 552 997 38 105 514 3321
Fragments arranged by size:
3321 997 552 514 105 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3837 3857 3043
Size: 794 20 4713
Fragments arranged by size:
4713 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5492
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4534 809
Size: 507 3218 1802
Fragments arranged by size:
3218 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2173 2974
Size: 54 1425 50 275 18 222 121 801
Cuts at: 2974 3863 5492 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 801 275 222 121 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2974 1537
Size: 275 1162 4090
Fragments arranged by size:
4090 1162 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3457 1353
Size: 2104 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2379 3590 4187 4974 5242 1239
Size: 393 527 220 1211 597 787 268 1524
Fragments arranged by size:
1524 1211 787 597 527 393 268 220
EcoO109I rG'GnC_Cy
Cuts at: 2593 2759 2593
Size: 166 5361
EcoRI G'AATT_C
Cuts at: 2836 3214 2836
Size: 378 5149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 5527
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 4674 1678
Size: 2996 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4168 4200 4242 4274 1373
Size: 32 135 32 2596 32 42 32 2626
Fragments arranged by size:
2626 2596 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2871 3117 669
Size: 988 281 933 246 3079
Fragments arranged by size:
3079 988 933 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KpnI G_GTAC'C
Cuts at: 2852 2852
Size: 5527
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2112 4641 4825 1305
Size: 807 2529 184 2007
Fragments arranged by size:
2529 2007 807 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3705
Fragments arranged by size:
3705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2733 3485 3844 4003 518
Size: 648 1089 478 752 359 159 2042
Fragments arranged by size:
2042 1089 752 648 478 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2943 3620 4768 5013 1606
Size: 458 879 677 1148 245 2120
Fragments arranged by size:
2120 1148 879 677 458 245
NaeI GCC'GGC
Cuts at: 2189 2189
Size: 5527
NcoI C'CATG_G
Cuts at: 513 1065 2719 513
Size: 552 1654 3321
Fragments arranged by size:
3321 1654 552
NdeI CA'TA_TG
Cuts at: 387 2383 387
Size: 1996 3531
NgoMIV G'CCGG_C
Cuts at: 2187 2187
Size: 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NsiI A_TGCA'T
Cuts at: 2740 2740
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2885 4430 1132
Size: 97 1656 1545 2229
Fragments arranged by size:
2229 1656 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5527
PciI A'CATG_T
Cuts at: 4426 4426
Size: 5527
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5527
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2593 2759 2593
Size: 166 5361
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5527
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PspOMI G'GGCC_C
Cuts at: 2029 2029
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2572 2879 830
Size: 1742 307 3478
Fragments arranged by size:
3478 1742 307
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
SacII CC_GC'GG
Cuts at: 2065 2065
Size: 5527
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SanDI GG'GwC_CC
Cuts at: 2593 2593
Size: 5527
SbfI CC_TGCA'GG
Cuts at: 2879 2879
Size: 5527
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SfcI C'TryA_G
Cuts at: 826 1045 2568 2875 4010 4691 4882 826
Size: 219 1523 307 1135 681 191 1471
Fragments arranged by size:
1523 1471 1135 681 307 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2854 1872
Size: 982 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3631 4532 4794 820
Size: 197 926 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 3451 5
Size: 47 3399 2081
Fragments arranged by size:
3399 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5527
StyI C'CwwG_G
Cuts at: 513 1065 2088 2719 2966 513
Size: 552 1023 631 247 3074
Fragments arranged by size:
3074 1023 631 552 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2363 2420 3554 3571 3724 3909 4354
Size: 1255 783 57 1134 17 153 185 445
Cuts at: 4354 325
Size: 1498
Fragments arranged by size:
1498 1255 1134 783 445 185 153 57
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3773 5485 96
Size: 274 80 33 51 1443 1796 1712 138
Fragments arranged by size:
1796 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5487 5519 5487
Size: 32 5495
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5527
XbaI T'CTAG_A
Cuts at: 2608 2863 2608
Size: 255 5272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2809 2809
Size: 5527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5527
XmaI C'CCGG_G
Cuts at: 1870 2852 1870
Size: 982 4545
XmnI GAAnn'nnTTC
Cuts at: 2339 3656 2339
Size: 1317 4210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3335 276
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI
SfiI SfoI SrfI SwaI