Restriction map for
pFN21AE6189
(Circular) (Six-base) MAPSORT of: pFN21AE6189.seq Check: 2708 from: 1 to: 5218
pFN21AE6189 5218 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3028 278
Size: 53 83 186 936 1492 2468
Fragments arranged by size:
2468 1492 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2561 1937
Size: 624 4594
Acc65I G'GTAC_C
Cuts at: 2539 2539
Size: 5218
AclI AA'CG_TT
Cuts at: 1563 3345 3718 1563
Size: 1782 373 3063
Fragments arranged by size:
3063 1782 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3281 4665 4933 1632
Size: 1649 1384 268 1917
Fragments arranged by size:
1917 1649 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5021
AflIII A'CryG_T
Cuts at: 1203 4117 5094 1203
Size: 2914 977 1327
Fragments arranged by size:
2914 1327 977
AgeI A'CCGG_T
Cuts at: 4049 4049
Size: 5218
AhdI GACnn_n'nnGTC
Cuts at: 3947 3947
Size: 5218
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5218
AlwNI CAG_nnn'CTG
Cuts at: 2961 4533 2961
Size: 1572 3646
ApaLI G'TGCA_C
Cuts at: 3275 4431 3275
Size: 1156 4062
ApoI r'AATT_y
Cuts at: 1493 2527 2753 2905 5014 1493
Size: 1034 226 152 2109 1697
Fragments arranged by size:
2109 1697 1034 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3772 160
Size: 872 2740 1606
Fragments arranged by size:
2740 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5218
AvaI C'yCGr_G
Cuts at: 1870 1943 2543 1870
Size: 73 600 4545
Fragments arranged by size:
4545 600 73
BamHI G'GATC_C
Cuts at: 2302 2548 2915 2302
Size: 246 367 4605
Fragments arranged by size:
4605 367 246
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2539 3994 618
Size: 325 217 627 752 1455 1842
Fragments arranged by size:
1842 1455 752 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2066 2509 2537 1391
Size: 675 443 28 4072
Fragments arranged by size:
4072 675 443 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2362 928
Size: 971 463 3784
Fragments arranged by size:
3784 971 463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3409 3443 1903
Size: 34 1472 34 3678
Fragments arranged by size:
3678 1472 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3110 4320 3110
Size: 1210 4008
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5218
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2315 3829 136
Size: 107 122 71 631 605 643 1514 1525
Fragments arranged by size:
1525 1514 643 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5213 5213
Size: 5218
BlpI GC'TnA_GC
Cuts at: 2635 2635
Size: 5218
Bme1580I G_kGCm'C
Cuts at: 1790 3279 4435 1790
Size: 1489 1156 2573
Fragments arranged by size:
2573 1489 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5218
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2512 2949 3907
Size: 416 53 83 399 24 1095 437 958
Cuts at: 3907 442
Size: 1753
Fragments arranged by size:
1753 1095 958 437 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3878 1239
Size: 2639 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 3343 4208 4506 2111
Size: 1232 865 298 2823
Fragments arranged by size:
2823 1232 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3881 882
Size: 617 2382 2219
Fragments arranged by size:
2382 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3009 493
Size: 711 1805 2702
Fragments arranged by size:
2702 1805 711
BsaBI GATnn'nnATC
Cuts at: 5212 5212
Size: 5218
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3025 3407 4042 275
Size: 53 83 186 936 1492 382 635 1451
Fragments arranged by size:
1492 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3651 4049 4323 4470 4881 3651
Size: 398 274 147 411 3988
Fragments arranged by size:
3988 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4993
Fragments arranged by size:
4993 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5218
BseYI C'CCAG_C
Cuts at: 4421 4917 4421
Size: 496 4722
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4645
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2668 3429 4457 1540
Size: 275 186 667 761 1028 2301
Fragments arranged by size:
2301 1028 761 667 275 186
BsiHKAI G_wGCw'C
Cuts at: 3279 3364 4435 3279
Size: 85 1071 4062
Fragments arranged by size:
4062 1071 85
BsmI GAATG_Cn'
Cuts at: 2729 2822 2729
Size: 93 5125
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2509 2537 3279 3364 4435 1391
Size: 399 276 443 28 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 276 85 28
BspEI T'CCGG_A
Cuts at: 4881 4881
Size: 5218
BspHI T'CATG_A
Cuts at: 3107 4837 3107
Size: 1730 3488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2573 844
Size: 1729 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3105 1394
Size: 429 1282 3507
Fragments arranged by size:
3507 1282 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2340 3713 3887 65
Size: 1183 1092 1373 174 1396
Fragments arranged by size:
1396 1373 1183 1092 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3862 4049 1700
Size: 110 2052 187 2869
Fragments arranged by size:
2869 2052 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5218
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5218
BssSI C'ACGA_G
Cuts at: 2205 3278 4290 2205
Size: 1073 1012 3133
Fragments arranged by size:
3133 1073 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5218
BstBI TT'CG_AA
Cuts at: 4022 4022
Size: 5218
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5218
BstYI r'GATC_y
Cuts at: 1972 2302 2548 2915 3300 3317 4769 4855 4867
Size: 330 246 367 385 17 1452 86 12
Cuts at: 4867 5213 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 330 246 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2313 513
Size: 552 939 309 3418
Fragments arranged by size:
3418 939 552 309
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4479
Fragments arranged by size:
4479 683 56
BtsI GCAGTG_nn'
Cuts at: 2153 2734 3528 3548 2153
Size: 581 794 20 3823
Fragments arranged by size:
3823 794 581 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5218
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5183
DraI TTT'AAA
Cuts at: 2522 2522
Size: 5218
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4225 809
Size: 507 2909 1802
Fragments arranged by size:
2909 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2665 3554 5183
Size: 54 1425 50 275 18 835 889 1629
Cuts at: 5183 8
Size: 43
Fragments arranged by size:
1629 1425 889 835 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2665 1537
Size: 275 853 4090
Fragments arranged by size:
4090 853 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3148 1353
Size: 1795 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3805 4179 4325 387
Size: 1064 138 2216 374 146 1280
Fragments arranged by size:
2216 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3281 3878 4665 4933 1239
Size: 393 1649 597 787 268 1524
Fragments arranged by size:
1649 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2527 2905 2527
Size: 378 4840
FspI TGC'GCA
Cuts at: 3724 3724
Size: 5218
HaeII r_GCGC'y
Cuts at: 1678 4365 1678
Size: 2687 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 3859 3891 3933
Size: 32 135 32 665 32 1590 32 42
Cuts at: 3933 3965 1373
Size: 32 2626
Fragments arranged by size:
2626 1590 665 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2562 2808 669
Size: 988 281 624 246 3079
Fragments arranged by size:
3079 988 624 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5218
HpaI GTT'AAC
Cuts at: 2808 2808
Size: 5218
KpnI G_GTAC'C
Cuts at: 2543 2543
Size: 5218
MfeI C'AATT_G
Cuts at: 2817 2817
Size: 5218
MluI A'CGCG_T
Cuts at: 5094 5094
Size: 5218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4332 4516 1305
Size: 3027 184 2007
Fragments arranged by size:
3027 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3396
Fragments arranged by size:
3396 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3176 3535 3694 518
Size: 648 2010 359 159 2042
Fragments arranged by size:
2042 2010 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2634 3311 4459 4704 1606
Size: 1028 677 1148 245 2120
Fragments arranged by size:
2120 1148 1028 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3727
Fragments arranged by size:
3727 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5218
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5218
NotI GC'GGCC_GC
Cuts at: 2665 2665
Size: 5218
NspI r_CATG'y
Cuts at: 1132 1229 2576 4121 1132
Size: 97 1347 1545 2229
Fragments arranged by size:
2229 1545 1347 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5218
PciI A'CATG_T
Cuts at: 4117 4117
Size: 5218
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5218
PfoI T'CCnGG_A
Cuts at: 2196 2196
Size: 5218
PmeI GTTT'AAAC
Cuts at: 2522 2522
Size: 5218
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 4800 4832 3287
Size: 32 1481 32 3673
Fragments arranged by size:
3673 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5218
PsiI TTA'TAA
Cuts at: 2788 2788
Size: 5218
PstI C_TGCA'G
Cuts at: 830 2570 830
Size: 1740 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5218
SalI G'TCGA_C
Cuts at: 1936 2560 1936
Size: 624 4594
SbfI CC_TGCA'GG
Cuts at: 2570 2570
Size: 5218
ScaI AGT'ACT
Cuts at: 3466 5178 3466
Size: 1712 3506
SexAI A'CCwGG_T
Cuts at: 2442 2442
Size: 5218
SfcI C'TryA_G
Cuts at: 826 1045 2079 2566 3701 4382 4573 826
Size: 219 1034 487 1135 681 191 1471
Fragments arranged by size:
1471 1135 1034 681 487 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5218
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5218
SmaI CCC'GGG
Cuts at: 1872 2545 1872
Size: 673 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 3322 4223 4485 820
Size: 197 926 147 1232 901 262 1553
Fragments arranged by size:
1553 1232 926 901 262 197 147
SnaBI TAC'GTA
Cuts at: 493 3009 493
Size: 2516 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5218
SphI G_CATG'C
Cuts at: 1132 2576 1132
Size: 1444 3774
SspI AAT'ATT
Cuts at: 5 52 3142 5
Size: 47 3090 2081
Fragments arranged by size:
3090 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5218
StyI C'CwwG_G
Cuts at: 513 1065 2004 2491 2657 513
Size: 552 939 487 166 3074
Fragments arranged by size:
3074 939 552 487 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3245 3262 3415 3600 4045 325
Size: 1255 1665 17 153 185 445 1498
Fragments arranged by size:
1665 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 3464 5176
Size: 274 80 33 51 1443 504 983 1712
Cuts at: 5176 96
Size: 138
Fragments arranged by size:
1712 1443 983 504 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5178 5210 5178
Size: 32 5186
Tth111I GACn'n_nGTC
Cuts at: 1316 2444 1316
Size: 1128 4090
XbaI T'CTAG_A
Cuts at: 2554 2554
Size: 5218
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5218
XmaI C'CCGG_G
Cuts at: 1870 2543 1870
Size: 673 4545
XmnI GAAnn'nnTTC
Cuts at: 3347 3347
Size: 5218
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3026 276
Size: 53 83 186 936 1492 2468
Fragments arranged by size:
2468 1492 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI