Restriction map for
pFN21AE6186
(Circular) (Six-base) MAPSORT of: pFN21AE6186.seq Check: 826 from: 1 to: 5209
pFN21AE6186 5209 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2134 2164 2134
Size: 30 5179
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3019 278
Size: 53 83 186 936 1483 2468
Fragments arranged by size:
2468 1483 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2552 1937
Size: 615 4594
Acc65I G'GTAC_C
Cuts at: 2530 2530
Size: 5209
AclI AA'CG_TT
Cuts at: 1563 3336 3709 1563
Size: 1773 373 3063
Fragments arranged by size:
3063 1773 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3272 4656 4924 1632
Size: 1640 1384 268 1917
Fragments arranged by size:
1917 1640 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5012
AflIII A'CryG_T
Cuts at: 1203 2373 4108 5085 1203
Size: 1170 1735 977 1327
Fragments arranged by size:
1735 1327 1170 977
AgeI A'CCGG_T
Cuts at: 4040 4040
Size: 5209
AhdI GACnn_n'nnGTC
Cuts at: 3938 3938
Size: 5209
AleI CACnn'nnGTG
Cuts at: 1166 2061 2144 1166
Size: 895 83 4231
Fragments arranged by size:
4231 895 83
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5209
AlwNI CAG_nnn'CTG
Cuts at: 2242 2952 4524 2242
Size: 710 1572 2927
Fragments arranged by size:
2927 1572 710
ApaLI G'TGCA_C
Cuts at: 3266 4422 3266
Size: 1156 4053
ApoI r'AATT_y
Cuts at: 1493 2518 2744 2896 5005 1493
Size: 1025 226 152 2109 1697
Fragments arranged by size:
2109 1697 1025 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3763 160
Size: 872 2731 1606
Fragments arranged by size:
2731 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5209
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2534 1870
Size: 73 89 502 4545
Fragments arranged by size:
4545 502 89 73
BamHI G'GATC_C
Cuts at: 2539 2906 2539
Size: 367 4842
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2279 2530 3985 618
Size: 325 217 627 372 120 251 1455 1842
Fragments arranged by size:
1842 1455 627 372 325 251 217 120
BanII G_rGCy'C
Cuts at: 1391 2528 1391
Size: 1137 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3400 3434 1903
Size: 34 50 34 1379 34 3678
Fragments arranged by size:
3678 1379 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3101 4311 3101
Size: 1210 3999
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5209
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 2357 2453
Size: 107 122 71 631 605 418 267 96
Cuts at: 2453 2486 3820 136
Size: 33 1334 1525
Fragments arranged by size:
1525 1334 631 605 418 267 122 107
96 71 33
BglII A'GATC_T
Cuts at: 5204 5204
Size: 5209
BlpI GC'TnA_GC
Cuts at: 2626 2626
Size: 5209
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2229 3270 4426 1790
Size: 397 42 1041 1156 2573
Fragments arranged by size:
2573 1156 1041 397 42
BmgBI CAC'GTC
Cuts at: 1318 2182 1318
Size: 864 4345
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2940 3898 442
Size: 416 53 83 399 24 1523 958 1753
Fragments arranged by size:
1753 1523 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2525 3869 1239
Size: 1286 1344 2579
Fragments arranged by size:
2579 1344 1286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3334 4199 4497 3334
Size: 865 298 4046
Fragments arranged by size:
4046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3872 882
Size: 617 2373 2219
Fragments arranged by size:
2373 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2224 3000 493
Size: 711 1020 776 2702
Fragments arranged by size:
2702 1020 776 711
BsaBI GATnn'nnATC
Cuts at: 2465 2471 5203 2465
Size: 6 2732 2471
Fragments arranged by size:
2732 2471 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3016 3398 4033 275
Size: 53 83 186 936 1483 382 635 1451
Fragments arranged by size:
1483 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2195 3642 4040 4314 4461 4872 2195
Size: 1447 398 274 147 411 2532
Fragments arranged by size:
2532 1447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4984
Fragments arranged by size:
4984 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5209
BseYI C'CCAG_C
Cuts at: 4412 4908 4412
Size: 496 4713
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 1163
Size: 573 624 4012
Fragments arranged by size:
4012 624 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2249 2659 3420 4448 1540
Size: 275 186 248 410 761 1028 2301
Fragments arranged by size:
2301 1028 761 410 275 248 186
BsiHKAI G_wGCw'C
Cuts at: 3270 3355 4426 3270
Size: 85 1071 4053
Fragments arranged by size:
4053 1071 85
BsmI GAATG_Cn'
Cuts at: 2720 2813 2720
Size: 93 5116
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2229 2528 3270 3355 4426 1391
Size: 399 397 42 299 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 299 85 42
BspEI T'CCGG_A
Cuts at: 4872 4872
Size: 5209
BspHI T'CATG_A
Cuts at: 3098 4828 3098
Size: 1730 3479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 2164 2227 2564 844
Size: 1290 30 63 337 3489
Fragments arranged by size:
3489 1290 337 63 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3096 1394
Size: 429 1273 3507
Fragments arranged by size:
3507 1273 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3704 3878 65
Size: 1183 2456 174 1396
Fragments arranged by size:
2456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3853 4040 1700
Size: 110 2043 187 2869
Fragments arranged by size:
2869 2043 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5209
BssSI C'ACGA_G
Cuts at: 3269 4281 3269
Size: 1012 4197
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 794
Size: 1448 3761
BstBI TT'CG_AA
Cuts at: 4013 4013
Size: 5209
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5209
BstYI r'GATC_y
Cuts at: 1972 2460 2466 2539 2906 3291 3308 4760 4846
Size: 488 6 73 367 385 17 1452 86
Cuts at: 4846 4858 5204 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 488 385 367 346 86 73
17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4657
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2299 487
Size: 683 56 1073 3397
Fragments arranged by size:
3397 1073 683 56
BtsI GCAGTG_nn'
Cuts at: 2325 2725 3519 3539 2325
Size: 400 794 20 3995
Fragments arranged by size:
3995 794 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5209
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5174
DraI TTT'AAA
Cuts at: 2513 2513
Size: 5209
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4216 809
Size: 507 2900 1802
Fragments arranged by size:
2900 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2246 2349
Size: 54 1425 50 275 18 211 205 103
Cuts at: 2349 2656 3545 5174 8
Size: 307 889 1629 43
Fragments arranged by size:
1629 1425 889 307 275 211 205 103
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2246 2656 1537
Size: 275 434 410 4090
Fragments arranged by size:
4090 434 410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2338 2349 3139 1353
Size: 985 11 790 3423
Fragments arranged by size:
3423 985 790 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2015 2042 2435 3796 4170 4316
Size: 1064 138 426 27 393 1361 374 146
Cuts at: 4316 387
Size: 1280
Fragments arranged by size:
1361 1280 1064 426 393 374 146 138
27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2525 3272 3869 4656 4924 1239
Size: 393 893 747 597 787 268 1524
Fragments arranged by size:
1524 893 787 747 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2495 2495
Size: 5209
EcoO109I rG'GnC_Cy
Cuts at: 2239 2488 2500 2239
Size: 249 12 4948
Fragments arranged by size:
4948 249 12
EcoRI G'AATT_C
Cuts at: 2518 2896 2518
Size: 378 4831
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 5209
FspI TGC'GCA
Cuts at: 3715 3715
Size: 5209
HaeII r_GCGC'y
Cuts at: 1678 4356 1678
Size: 2678 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3850 3882 3924 3956 1373
Size: 32 135 32 2278 32 42 32 2626
Fragments arranged by size:
2626 2278 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2553 2799 669
Size: 988 281 615 246 3079
Fragments arranged by size:
3079 988 615 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5209
HpaI GTT'AAC
Cuts at: 2799 2799
Size: 5209
KpnI G_GTAC'C
Cuts at: 2534 2534
Size: 5209
MfeI C'AATT_G
Cuts at: 2808 2808
Size: 5209
MluI A'CGCG_T
Cuts at: 5085 5085
Size: 5209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 4323 4507 1305
Size: 1053 1965 184 2007
Fragments arranged by size:
2007 1965 1053 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3387
Fragments arranged by size:
3387 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2061 2144 2318 3167 3526 3685 518
Size: 648 895 83 174 849 359 159 2042
Fragments arranged by size:
2042 895 849 648 359 174 159 83
MspA1I CmG'CkG
Cuts at: 1606 2307 2438 2625 3302 4450 4695 1606
Size: 701 131 187 677 1148 245 2120
Fragments arranged by size:
2120 1148 701 677 245 187 131
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4657
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5209
NotI GC'GGCC_GC
Cuts at: 2656 2656
Size: 5209
NspI r_CATG'y
Cuts at: 1132 1229 2270 2377 2567 4112 1132
Size: 97 1041 107 190 1545 2229
Fragments arranged by size:
2229 1545 1041 190 107 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5209
PciI A'CATG_T
Cuts at: 2373 4108 2373
Size: 1735 3474
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5209
PmeI GTTT'AAAC
Cuts at: 2513 2513
Size: 5209
PmlI CAC'GTG
Cuts at: 2224 2224
Size: 5209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2315 2325 2347 3278 3310 4791 4823 2293
Size: 22 10 22 931 32 1481 32 2679
Fragments arranged by size:
2679 1481 931 32 32 22 22 10
PpuMI rG'GwC_Cy
Cuts at: 2239 2239
Size: 5209
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5209
PsiI TTA'TAA
Cuts at: 2779 2779
Size: 5209
PstI C_TGCA'G
Cuts at: 830 2238 2561 830
Size: 1408 323 3478
Fragments arranged by size:
3478 1408 323
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5209
PvuII CAG'CTG
Cuts at: 2438 2438
Size: 5209
SalI G'TCGA_C
Cuts at: 1936 2551 1936
Size: 615 4594
SbfI CC_TGCA'GG
Cuts at: 2561 2561
Size: 5209
ScaI AGT'ACT
Cuts at: 3457 5169 3457
Size: 1712 3497
SfcI C'TryA_G
Cuts at: 826 1045 2234 2557 3692 4373 4564 826
Size: 219 1189 323 1135 681 191 1471
Fragments arranged by size:
1471 1189 1135 681 323 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2357 2486 2357
Size: 129 5080
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5209
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5209
SmaI CCC'GGG
Cuts at: 1872 2034 2536 1872
Size: 162 502 4545
Fragments arranged by size:
4545 502 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3313 4214 4476 820
Size: 197 926 1370 901 262 1553
Fragments arranged by size:
1553 1370 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3000 493
Size: 2507 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5209
SphI G_CATG'C
Cuts at: 1132 2270 2567 1132
Size: 1138 297 3774
Fragments arranged by size:
3774 1138 297
SspI AAT'ATT
Cuts at: 5 52 3133 5
Size: 47 3081 2081
Fragments arranged by size:
3081 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5209
StyI C'CwwG_G
Cuts at: 513 1065 2394 2648 513
Size: 552 1329 254 3074
Fragments arranged by size:
3074 1329 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3236 3253 3406 3591 4036 325
Size: 1255 1656 17 153 185 445 1498
Fragments arranged by size:
1656 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3455 5167 96
Size: 274 80 33 51 1443 1478 1712 138
Fragments arranged by size:
1712 1478 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5169 5201 5169
Size: 32 5177
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5209
XbaI T'CTAG_A
Cuts at: 2545 2545
Size: 5209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2488 2401
Size: 87 5122
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5209
XmaI C'CCGG_G
Cuts at: 1870 2032 2534 1870
Size: 162 502 4545
Fragments arranged by size:
4545 502 162
XmnI GAAnn'nnTTC
Cuts at: 3338 3338
Size: 5209
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3017 276
Size: 53 83 186 936 1483 2468
Fragments arranged by size:
2468 1483 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfoI SrfI
SwaI