Restriction map for
pFN21AE6182
(Circular) (Six-base) MAPSORT of: pFN21AE6182.seq Check: 3224 from: 1 to: 5131
pFN21AE6182 5131 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2228 2228
Size: 5131
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2941 278
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 1937
Size: 537 4594
Acc65I G'GTAC_C
Cuts at: 2452 2452
Size: 5131
AclI AA'CG_TT
Cuts at: 1563 3258 3631 1563
Size: 1695 373 3063
Fragments arranged by size:
3063 1695 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 2397 3194 4578 4846 1632
Size: 719 46 797 1384 268 1917
Fragments arranged by size:
1917 1384 797 719 268 46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4934
AflIII A'CryG_T
Cuts at: 1203 4030 5007 1203
Size: 2827 977 1327
Fragments arranged by size:
2827 1327 977
AgeI A'CCGG_T
Cuts at: 3962 3962
Size: 5131
AhdI GACnn_n'nnGTC
Cuts at: 3860 3860
Size: 5131
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5131
AlwNI CAG_nnn'CTG
Cuts at: 2243 2341 2874 4446 2243
Size: 98 533 1572 2928
Fragments arranged by size:
2928 1572 533 98
ApaI G_GGCC'C
Cuts at: 2149 2149
Size: 5131
ApaLI G'TGCA_C
Cuts at: 3188 4344 3188
Size: 1156 3975
ApoI r'AATT_y
Cuts at: 1493 2225 2440 2666 2818 4927 1493
Size: 732 215 226 152 2109 1697
Fragments arranged by size:
2109 1697 732 226 215 152
AseI AT'TA_AT
Cuts at: 160 1032 3685 160
Size: 872 2653 1606
Fragments arranged by size:
2653 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
AvaI C'yCGr_G
Cuts at: 1870 1943 2456 1870
Size: 73 513 4545
Fragments arranged by size:
4545 513 73
BamHI G'GATC_C
Cuts at: 2054 2461 2828 2054
Size: 407 367 4357
Fragments arranged by size:
4357 407 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2452 3907 618
Size: 325 217 627 665 1455 1842
Fragments arranged by size:
1842 1455 665 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2129 2149 2450 1391
Size: 738 20 301 4072
Fragments arranged by size:
4072 738 301 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3322 3356 1903
Size: 34 1385 34 3678
Fragments arranged by size:
3678 1385 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3023 4233 3023
Size: 1210 3921
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5131
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3742 136
Size: 107 122 71 631 605 2070 1525
Fragments arranged by size:
2070 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5126 5126
Size: 5131
BlpI GC'TnA_GC
Cuts at: 2548 2548
Size: 5131
Bme1580I G_kGCm'C
Cuts at: 1790 2149 3192 4348 1790
Size: 359 1043 1156 2573
Fragments arranged by size:
2573 1156 1043 359
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5131
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2862 3820 442
Size: 416 53 83 399 24 1445 958 1753
Fragments arranged by size:
1753 1445 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2139 3791 1239
Size: 900 1652 2579
Fragments arranged by size:
2579 1652 900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3256 4121 4419 3256
Size: 865 298 3968
Fragments arranged by size:
3968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2393 3794 882
Size: 617 894 1401 2219
Fragments arranged by size:
2219 1401 894 617
BsaAI yAC'GTr
Cuts at: 493 1204 2922 493
Size: 711 1718 2702
Fragments arranged by size:
2702 1718 711
BsaBI GATnn'nnATC
Cuts at: 5125 5125
Size: 5131
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2938 3320 3955 275
Size: 53 83 186 936 1405 382 635 1451
Fragments arranged by size:
1451 1405 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3564 3962 4236 4383 4794 3564
Size: 398 274 147 411 3901
Fragments arranged by size:
3901 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4906
Fragments arranged by size:
4906 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5131
BseYI C'CCAG_C
Cuts at: 4334 4830 4334
Size: 496 4635
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2014 2256 1163
Size: 573 278 242 4038
Fragments arranged by size:
4038 573 278 242
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2581 3342 4370 1540
Size: 275 186 580 761 1028 2301
Fragments arranged by size:
2301 1028 761 580 275 186
BsiHKAI G_wGCw'C
Cuts at: 3192 3277 4348 3192
Size: 85 1071 3975
Fragments arranged by size:
3975 1071 85
BsmI GAATG_Cn'
Cuts at: 2642 2735 2642
Size: 93 5038
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2149 2450 3192 3277 4348 1391
Size: 399 339 20 301 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 339 301 85 20
BspEI T'CCGG_A
Cuts at: 4794 4794
Size: 5131
BspHI T'CATG_A
Cuts at: 3020 4750 3020
Size: 1730 3401
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2228 2486 844
Size: 1384 258 3489
Fragments arranged by size:
3489 1384 258
BsrBI CCG'CTC
Cuts at: 1394 1823 3018 1394
Size: 429 1195 3507
Fragments arranged by size:
3507 1195 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3626 3800 65
Size: 1183 2378 174 1396
Fragments arranged by size:
2378 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3775 3962 1700
Size: 110 1965 187 2869
Fragments arranged by size:
2869 1965 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5131
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5131
BssSI C'ACGA_G
Cuts at: 3191 4203 3191
Size: 1012 4119
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2243 794
Size: 1449 3682
BstBI TT'CG_AA
Cuts at: 3935 3935
Size: 5131
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5131
BstYI r'GATC_y
Cuts at: 1972 2054 2461 2828 3213 3230 4682 4768 4780
Size: 82 407 367 385 17 1452 86 12
Cuts at: 4780 5126 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 407 385 367 346 86 82
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2427 513
Size: 552 1362 3217
Fragments arranged by size:
3217 1362 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4392
Fragments arranged by size:
4392 683 56
BtsI GCAGTG_nn'
Cuts at: 2647 3441 3461 2647
Size: 794 20 4317
Fragments arranged by size:
4317 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5096
DraI TTT'AAA
Cuts at: 2435 2435
Size: 5131
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4138 809
Size: 507 2822 1802
Fragments arranged by size:
2822 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2578 3467 5096
Size: 54 1425 50 275 18 748 889 1629
Cuts at: 5096 8
Size: 43
Fragments arranged by size:
1629 1425 889 748 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2578 1537
Size: 275 766 4090
Fragments arranged by size:
4090 766 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3061 1353
Size: 1708 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3718 4092 4238 387
Size: 1064 138 2129 374 146 1280
Fragments arranged by size:
2129 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2351 2397 3194 3791 4578 4846
Size: 393 507 212 46 797 597 787 268
Cuts at: 4846 1239
Size: 1524
Fragments arranged by size:
1524 797 787 597 507 393 268 212
46
EcoO109I rG'GnC_Cy
Cuts at: 2145 2145
Size: 5131
EcoRI G'AATT_C
Cuts at: 2440 2818 2440
Size: 378 4753
FspI TGC'GCA
Cuts at: 3637 3637
Size: 5131
HaeII r_GCGC'y
Cuts at: 1678 4278 1678
Size: 2600 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3772 3804 3846 3878 1373
Size: 32 135 32 2200 32 42 32 2626
Fragments arranged by size:
2626 2200 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2475 2721 669
Size: 988 281 537 246 3079
Fragments arranged by size:
3079 988 537 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5131
HpaI GTT'AAC
Cuts at: 2721 2721
Size: 5131
KpnI G_GTAC'C
Cuts at: 2456 2456
Size: 5131
MfeI C'AATT_G
Cuts at: 2730 2730
Size: 5131
MluI A'CGCG_T
Cuts at: 5007 5007
Size: 5131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4245 4429 1305
Size: 2940 184 2007
Fragments arranged by size:
2940 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3309
Fragments arranged by size:
3309 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3089 3448 3607 518
Size: 648 1923 359 159 2042
Fragments arranged by size:
2042 1923 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2341 2547 3224 4372 4617 1606
Size: 735 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 735 677 245 206
NcoI C'CATG_G
Cuts at: 513 1065 2427 513
Size: 552 1362 3217
Fragments arranged by size:
3217 1362 552
NdeI CA'TA_TG
Cuts at: 387 2350 387
Size: 1963 3168
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5131
NotI GC'GGCC_GC
Cuts at: 2578 2578
Size: 5131
NspI r_CATG'y
Cuts at: 1132 1229 2489 4034 1132
Size: 97 1260 1545 2229
Fragments arranged by size:
2229 1545 1260 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5131
PciI A'CATG_T
Cuts at: 4030 4030
Size: 5131
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5131
PmeI GTTT'AAAC
Cuts at: 2435 2435
Size: 5131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 4713 4745 3200
Size: 32 1481 32 3586
Fragments arranged by size:
3586 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5131
PsiI TTA'TAA
Cuts at: 2701 2701
Size: 5131
PspOMI G'GGCC_C
Cuts at: 2145 2145
Size: 5131
PstI C_TGCA'G
Cuts at: 830 2483 830
Size: 1653 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5131
PvuII CAG'CTG
Cuts at: 2341 2341
Size: 5131
SalI G'TCGA_C
Cuts at: 1936 2473 1936
Size: 537 4594
SbfI CC_TGCA'GG
Cuts at: 2483 2483
Size: 5131
ScaI AGT'ACT
Cuts at: 3379 5091 3379
Size: 1712 3419
SfcI C'TryA_G
Cuts at: 826 1045 2479 3614 4295 4486 826
Size: 219 1434 1135 681 191 1471
Fragments arranged by size:
1471 1434 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5131
SmaI CCC'GGG
Cuts at: 1872 2458 1872
Size: 586 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3235 4136 4398 820
Size: 197 926 1292 901 262 1553
Fragments arranged by size:
1553 1292 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2922 493
Size: 2429 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5131
SphI G_CATG'C
Cuts at: 1132 2489 1132
Size: 1357 3774
SspI AAT'ATT
Cuts at: 5 52 3055 5
Size: 47 3003 2081
Fragments arranged by size:
3003 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5131
StyI C'CwwG_G
Cuts at: 513 1065 2427 2570 513
Size: 552 1362 143 3074
Fragments arranged by size:
3074 1362 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3158 3175 3328 3513 3958 325
Size: 1255 1578 17 153 185 445 1498
Fragments arranged by size:
1578 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3377 5089 96
Size: 274 80 33 51 1443 1400 1712 138
Fragments arranged by size:
1712 1443 1400 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5091 5123 5091
Size: 32 5099
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5131
XbaI T'CTAG_A
Cuts at: 2467 2467
Size: 5131
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5131
XmaI C'CCGG_G
Cuts at: 1870 2456 1870
Size: 586 4545
XmnI GAAnn'nnTTC
Cuts at: 2323 3260 2323
Size: 937 4194
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2939 276
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI