Restriction map for
pFN21AE6172
(Circular) (Six-base) MAPSORT of: pFN21AE6172.seq Check: 4355 from: 1 to: 4951
pFN21AE6172 4951 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2761 278
Size: 53 83 186 936 1225 2468
Fragments arranged by size:
2468 1225 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2294 1937
Size: 357 4594
Acc65I G'GTAC_C
Cuts at: 2272 2272
Size: 4951
AclI AA'CG_TT
Cuts at: 1563 3078 3451 1563
Size: 1515 373 3063
Fragments arranged by size:
3063 1515 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 3014 4398 4666 1632
Size: 585 797 1384 268 1917
Fragments arranged by size:
1917 1384 797 585 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4754
AflIII A'CryG_T
Cuts at: 1203 3850 4827 1203
Size: 2647 977 1327
Fragments arranged by size:
2647 1327 977
AgeI A'CCGG_T
Cuts at: 3782 3782
Size: 4951
AhdI GACnn_n'nnGTC
Cuts at: 3680 3680
Size: 4951
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4951
AlwNI CAG_nnn'CTG
Cuts at: 2694 4266 2694
Size: 1572 3379
ApaLI G'TGCA_C
Cuts at: 3008 4164 3008
Size: 1156 3795
ApoI r'AATT_y
Cuts at: 1493 2260 2486 2638 4747 1493
Size: 767 226 152 2109 1697
Fragments arranged by size:
2109 1697 767 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3505 160
Size: 872 2473 1606
Fragments arranged by size:
2473 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4951
AvaI C'yCGr_G
Cuts at: 1870 1943 2276 1870
Size: 73 333 4545
Fragments arranged by size:
4545 333 73
BamHI G'GATC_C
Cuts at: 2281 2648 2281
Size: 367 4584
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 2272 3727 618
Size: 325 217 627 365 120 1455 1842
Fragments arranged by size:
1842 1455 627 365 325 217 120
BanII G_rGCy'C
Cuts at: 1391 2270 1391
Size: 879 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 928
Size: 971 187 3793
Fragments arranged by size:
3793 971 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3142 3176 1903
Size: 34 1205 34 3678
Fragments arranged by size:
3678 1205 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2843 4053 2843
Size: 1210 3741
BclI T'GATC_A
Cuts at: 1548 2046 1548
Size: 498 4453
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3562 136
Size: 107 122 71 631 605 1890 1525
Fragments arranged by size:
1890 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4946 4946
Size: 4951
BlpI GC'TnA_GC
Cuts at: 2368 2368
Size: 4951
Bme1580I G_kGCm'C
Cuts at: 1790 3012 4168 1790
Size: 1222 1156 2573
Fragments arranged by size:
2573 1222 1156
BmgBI CAC'GTC
Cuts at: 1318 2092 1318
Size: 774 4177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2682 3640 442
Size: 416 53 83 399 24 1265 958 1753
Fragments arranged by size:
1753 1265 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3611 1239
Size: 2372 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3076 3941 4239 3076
Size: 865 298 3788
Fragments arranged by size:
3788 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3614 882
Size: 617 2115 2219
Fragments arranged by size:
2219 2115 617
BsaAI yAC'GTr
Cuts at: 493 1204 2742 493
Size: 711 1538 2702
Fragments arranged by size:
2702 1538 711
BsaBI GATnn'nnATC
Cuts at: 4945 4945
Size: 4951
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2758 3140 3775 275
Size: 53 83 186 936 1225 382 635 1451
Fragments arranged by size:
1451 1225 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3384 3782 4056 4203 4614 3384
Size: 398 274 147 411 3721
Fragments arranged by size:
3721 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4726
Fragments arranged by size:
4726 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4951
BseYI C'CCAG_C
Cuts at: 4154 4650 4154
Size: 496 4455
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2401 3162 4190 1540
Size: 275 186 400 761 1028 2301
Fragments arranged by size:
2301 1028 761 400 275 186
BsiHKAI G_wGCw'C
Cuts at: 3012 3097 4168 3012
Size: 85 1071 3795
Fragments arranged by size:
3795 1071 85
BsmI GAATG_Cn'
Cuts at: 2127 2462 2555 2127
Size: 335 93 4523
Fragments arranged by size:
4523 335 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2270 3012 3097 4168 1391
Size: 399 480 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 399 85
BspEI T'CCGG_A
Cuts at: 4614 4614
Size: 4951
BspHI T'CATG_A
Cuts at: 2840 4570 2840
Size: 1730 3221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2306 844
Size: 1462 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2838 1394
Size: 429 1015 3507
Fragments arranged by size:
3507 1015 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3446 3620 65
Size: 1183 2198 174 1396
Fragments arranged by size:
2198 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3595 3782 1700
Size: 110 1785 187 2869
Fragments arranged by size:
2869 1785 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4951
BssHII G'CGCG_C
Cuts at: 1925 2077 1925
Size: 152 4799
BssSI C'ACGA_G
Cuts at: 3011 4023 3011
Size: 1012 3939
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4951
BstBI TT'CG_AA
Cuts at: 3755 3755
Size: 4951
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4951
BstYI r'GATC_y
Cuts at: 1972 2210 2281 2648 3033 3050 4502 4588 4600
Size: 238 71 367 385 17 1452 86 12
Cuts at: 4600 4946 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 238 86 71
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3460
Fragments arranged by size:
3460 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4212
Fragments arranged by size:
4212 683 56
BtsI GCAGTG_nn'
Cuts at: 2467 3261 3281 2467
Size: 794 20 4137
Fragments arranged by size:
4137 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4951
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4916
DraI TTT'AAA
Cuts at: 2255 2255
Size: 4951
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3958 809
Size: 507 2642 1802
Fragments arranged by size:
2642 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2398 3287 4916
Size: 54 1425 50 275 18 568 889 1629
Cuts at: 4916 8
Size: 43
Fragments arranged by size:
1629 1425 889 568 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2398 1537
Size: 275 586 4090
Fragments arranged by size:
4090 586 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2193 2881 1353
Size: 840 688 3423
Fragments arranged by size:
3423 840 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3538 3912 4058 387
Size: 1064 138 1949 374 146 1280
Fragments arranged by size:
1949 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 3014 3611 4398 4666 1239
Size: 393 585 797 597 787 268 1524
Fragments arranged by size:
1524 797 787 597 585 393 268
EcoRI G'AATT_C
Cuts at: 2260 2638 2260
Size: 378 4573
FspI TGC'GCA
Cuts at: 3457 3457
Size: 4951
HaeII r_GCGC'y
Cuts at: 1678 4098 1678
Size: 2420 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2241 3592 3624 3666
Size: 32 135 32 637 32 1351 32 42
Cuts at: 3666 3698 1373
Size: 32 2626
Fragments arranged by size:
2626 1351 637 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2295 2541 669
Size: 988 281 357 246 3079
Fragments arranged by size:
3079 988 357 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4951
HpaI GTT'AAC
Cuts at: 2541 2541
Size: 4951
KpnI G_GTAC'C
Cuts at: 2276 2276
Size: 4951
MfeI C'AATT_G
Cuts at: 2550 2550
Size: 4951
MluI A'CGCG_T
Cuts at: 4827 4827
Size: 4951
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4065 4249 1305
Size: 2760 184 2007
Fragments arranged by size:
2760 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3129
Fragments arranged by size:
3129 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2909 3268 3427 518
Size: 648 1743 359 159 2042
Fragments arranged by size:
2042 1743 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2231 2367 3044 4192 4437 1606
Size: 625 136 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 625 245 136
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3460
Fragments arranged by size:
3460 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4951
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4951
NotI GC'GGCC_GC
Cuts at: 2398 2398
Size: 4951
NspI r_CATG'y
Cuts at: 1132 1229 2045 2309 3854 1132
Size: 97 816 264 1545 2229
Fragments arranged by size:
2229 1545 816 264 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4951
PciI A'CATG_T
Cuts at: 3850 3850
Size: 4951
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4951
PmeI GTTT'AAAC
Cuts at: 2255 2255
Size: 4951
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3020 3052 4533 4565 3020
Size: 32 1481 32 3406
Fragments arranged by size:
3406 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4951
PsiI TTA'TAA
Cuts at: 2521 2521
Size: 4951
PstI C_TGCA'G
Cuts at: 830 2303 830
Size: 1473 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4951
PvuII CAG'CTG
Cuts at: 2231 2231
Size: 4951
SalI G'TCGA_C
Cuts at: 1936 2293 1936
Size: 357 4594
SbfI CC_TGCA'GG
Cuts at: 2303 2303
Size: 4951
ScaI AGT'ACT
Cuts at: 3199 4911 3199
Size: 1712 3239
SfcI C'TryA_G
Cuts at: 826 1045 2053 2299 3434 4115 4306 826
Size: 219 1008 246 1135 681 191 1471
Fragments arranged by size:
1471 1135 1008 681 246 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4951
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4951
SmaI CCC'GGG
Cuts at: 1872 2278 1872
Size: 406 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3055 3956 4218 820
Size: 197 926 1112 901 262 1553
Fragments arranged by size:
1553 1112 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2742 493
Size: 2249 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4951
SphI G_CATG'C
Cuts at: 1132 2309 1132
Size: 1177 3774
SspI AAT'ATT
Cuts at: 5 52 2875 5
Size: 47 2823 2081
Fragments arranged by size:
2823 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4951
StyI C'CwwG_G
Cuts at: 513 1065 2004 2390 513
Size: 552 939 386 3074
Fragments arranged by size:
3074 939 552 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2978 2995 3148 3333 3778 325
Size: 1255 1398 17 153 185 445 1498
Fragments arranged by size:
1498 1398 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3197 4909 96
Size: 274 80 33 51 1443 1220 1712 138
Fragments arranged by size:
1712 1443 1220 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4911 4943 4911
Size: 32 4919
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4951
XbaI T'CTAG_A
Cuts at: 2287 2287
Size: 4951
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4951
XmaI C'CCGG_G
Cuts at: 1870 2276 1870
Size: 406 4545
XmnI GAAnn'nnTTC
Cuts at: 3080 3080
Size: 4951
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2759 276
Size: 53 83 186 936 1225 2468
Fragments arranged by size:
2468 1225 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI