Restriction map for
pFN21AE6168
(Circular) (Six-base) MAPSORT of: pFN21AE6168.seq Check: 3344 from: 1 to: 5128
pFN21AE6168 5128 bp
With 182 enzymes: SgfI *
December 8, 2014 13:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2938 278
Size: 53 83 186 936 1402 2468
Fragments arranged by size:
2468 1402 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2471 1937
Size: 534 4594
Acc65I G'GTAC_C
Cuts at: 2277 2449 2277
Size: 172 4956
AclI AA'CG_TT
Cuts at: 1563 3255 3628 1563
Size: 1692 373 3063
Fragments arranged by size:
3063 1692 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3191 4575 4843 1632
Size: 1559 1384 268 1917
Fragments arranged by size:
1917 1559 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4931
AflIII A'CryG_T
Cuts at: 1203 4027 5004 1203
Size: 2824 977 1327
Fragments arranged by size:
2824 1327 977
AgeI A'CCGG_T
Cuts at: 3959 3959
Size: 5128
AhdI GACnn_n'nnGTC
Cuts at: 3857 3857
Size: 5128
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5128
AlwNI CAG_nnn'CTG
Cuts at: 2871 4443 2871
Size: 1572 3556
ApaLI G'TGCA_C
Cuts at: 3185 4341 3185
Size: 1156 3972
ApoI r'AATT_y
Cuts at: 1493 2437 2663 2815 4924 1493
Size: 944 226 152 2109 1697
Fragments arranged by size:
2109 1697 944 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3682 160
Size: 872 2650 1606
Fragments arranged by size:
2650 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5128
AvaI C'yCGr_G
Cuts at: 1870 1943 2453 1870
Size: 73 510 4545
Fragments arranged by size:
4545 510 73
BamHI G'GATC_C
Cuts at: 2458 2825 2458
Size: 367 4761
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2277 2449 3904 618
Size: 325 217 627 490 172 1455 1842
Fragments arranged by size:
1842 1455 627 490 325 217 172
BanII G_rGCy'C
Cuts at: 1391 2447 1391
Size: 1056 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4157
BbvCI CC'TCA_GC
Cuts at: 2252 2263 2252
Size: 11 5117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2288 2322 3319 3353 1903
Size: 34 351 34 997 34 3678
Fragments arranged by size:
3678 997 351 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3020 4230 3020
Size: 1210 3918
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5128
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3739 136
Size: 107 122 71 631 605 2067 1525
Fragments arranged by size:
2067 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5123 5123
Size: 5128
BlpI GC'TnA_GC
Cuts at: 2545 2545
Size: 5128
Bme1580I G_kGCm'C
Cuts at: 1790 3189 4345 1790
Size: 1399 1156 2573
Fragments arranged by size:
2573 1399 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5128
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2859 3817 442
Size: 416 53 83 399 24 1442 958 1753
Fragments arranged by size:
1753 1442 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2223 3788 1239
Size: 905 79 1565 2579
Fragments arranged by size:
2579 1565 905 79
Bpu10I CC'TnA_GC
Cuts at: 2252 2263 2310 2252
Size: 11 47 5070
Fragments arranged by size:
5070 47 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3253 4118 4416 3253
Size: 865 298 3965
Fragments arranged by size:
3965 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3791 882
Size: 617 2292 2219
Fragments arranged by size:
2292 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2919 493
Size: 711 1715 2702
Fragments arranged by size:
2702 1715 711
BsaBI GATnn'nnATC
Cuts at: 5122 5122
Size: 5128
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2935 3317 3952 275
Size: 53 83 186 936 1402 382 635 1451
Fragments arranged by size:
1451 1402 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3561 3959 4233 4380 4791 3561
Size: 398 274 147 411 3898
Fragments arranged by size:
3898 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2349 2351 2379 1178
Size: 30 165 30 918 28 2 28 3927
Fragments arranged by size:
3927 918 165 30 30 28 28 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2248 2251 2375 1186
Size: 1062 3 124 3939
Fragments arranged by size:
3939 1062 124 3
BseYI C'CCAG_C
Cuts at: 2340 2349 2402 4331 4827 2340
Size: 9 53 1929 496 2641
Fragments arranged by size:
2641 1929 496 53 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2122 1163
Size: 573 386 4169
Fragments arranged by size:
4169 573 386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2578 3339 4367 1540
Size: 275 186 577 761 1028 2301
Fragments arranged by size:
2301 1028 761 577 275 186
BsiHKAI G_wGCw'C
Cuts at: 3189 3274 4345 3189
Size: 85 1071 3972
Fragments arranged by size:
3972 1071 85
BsmI GAATG_Cn'
Cuts at: 2639 2732 2639
Size: 93 5035
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2447 3189 3274 4345 1391
Size: 399 657 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 399 85
BspEI T'CCGG_A
Cuts at: 4791 4791
Size: 5128
BspHI T'CATG_A
Cuts at: 3017 4747 3017
Size: 1730 3398
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2483 844
Size: 1639 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2275 3015 1394
Size: 429 452 740 3507
Fragments arranged by size:
3507 740 452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3623 3797 65
Size: 1183 2375 174 1396
Fragments arranged by size:
2375 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3772 3959 1700
Size: 110 1962 187 2869
Fragments arranged by size:
2869 1962 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5128
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5128
BssSI C'ACGA_G
Cuts at: 3188 4200 3188
Size: 1012 4116
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5128
BstBI TT'CG_AA
Cuts at: 3932 3932
Size: 5128
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5128
BstYI r'GATC_y
Cuts at: 1972 2458 2825 3210 3227 4679 4765 4777 5123
Size: 486 367 385 17 1452 86 12 346
Cuts at: 5123 1972
Size: 1977
Fragments arranged by size:
1977 1452 486 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2387 2387
Size: 5128
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 513
Size: 552 939 183 3454
Fragments arranged by size:
3454 939 552 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4389
Fragments arranged by size:
4389 683 56
BtsI GCAGTG_nn'
Cuts at: 2134 2644 3438 3458 2134
Size: 510 794 20 3804
Fragments arranged by size:
3804 794 510 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5093
DraI TTT'AAA
Cuts at: 2432 2432
Size: 5128
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4135 809
Size: 507 2819 1802
Fragments arranged by size:
2819 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2575 3464
Size: 54 1425 50 275 18 465 280 889
Cuts at: 3464 5093 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 465 280 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2575 1537
Size: 275 763 4090
Fragments arranged by size:
4090 763 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3058 1353
Size: 1705 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3715 4089 4235 387
Size: 1064 138 2126 374 146 1280
Fragments arranged by size:
2126 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2223 3191 3788 4575 4843 1239
Size: 393 512 79 968 597 787 268 1524
Fragments arranged by size:
1524 968 787 597 512 393 268 79
EcoNI CCTnn'n_nnAGG
Cuts at: 2297 2373 2297
Size: 76 5052
EcoO109I rG'GnC_Cy
Cuts at: 2248 2248
Size: 5128
EcoRI G'AATT_C
Cuts at: 2437 2815 2437
Size: 378 4750
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 5128
FspI TGC'GCA
Cuts at: 3634 3634
Size: 5128
HaeII r_GCGC'y
Cuts at: 1678 4275 1678
Size: 2597 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 3769 3801 3843
Size: 32 135 32 749 32 1416 32 42
Cuts at: 3843 3875 1373
Size: 32 2626
Fragments arranged by size:
2626 1416 749 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2472 2718 669
Size: 988 281 534 246 3079
Fragments arranged by size:
3079 988 534 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5128
HpaI GTT'AAC
Cuts at: 2718 2718
Size: 5128
KpnI G_GTAC'C
Cuts at: 2281 2453 2281
Size: 172 4956
MfeI C'AATT_G
Cuts at: 2727 2727
Size: 5128
MluI A'CGCG_T
Cuts at: 5004 5004
Size: 5128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4242 4426 1305
Size: 2937 184 2007
Fragments arranged by size:
2937 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3306
Fragments arranged by size:
3306 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3086 3445 3604 518
Size: 648 1920 359 159 2042
Fragments arranged by size:
2042 1920 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2544 3221 4369 4614 1606
Size: 938 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3637
Fragments arranged by size:
3637 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5128
NotI GC'GGCC_GC
Cuts at: 2575 2575
Size: 5128
NspI r_CATG'y
Cuts at: 1132 1229 2486 4031 1132
Size: 97 1257 1545 2229
Fragments arranged by size:
2229 1545 1257 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5128
PciI A'CATG_T
Cuts at: 4027 4027
Size: 5128
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 1108
Size: 1215 3913
PmeI GTTT'AAAC
Cuts at: 2432 2432
Size: 5128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 4710 4742 3197
Size: 32 1481 32 3583
Fragments arranged by size:
3583 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2248
Size: 5128
PshAI GACnn'nnGTC
Cuts at: 1613 2246 1613
Size: 633 4495
PsiI TTA'TAA
Cuts at: 2698 2698
Size: 5128
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2270 2302 2270
Size: 32 5096
PstI C_TGCA'G
Cuts at: 830 2480 830
Size: 1650 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5128
SalI G'TCGA_C
Cuts at: 1936 2470 1936
Size: 534 4594
SbfI CC_TGCA'GG
Cuts at: 2480 2480
Size: 5128
ScaI AGT'ACT
Cuts at: 3376 5088 3376
Size: 1712 3416
SfcI C'TryA_G
Cuts at: 826 1045 2476 3611 4292 4483 826
Size: 219 1431 1135 681 191 1471
Fragments arranged by size:
1471 1431 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5128
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5128
SmaI CCC'GGG
Cuts at: 1872 2455 1872
Size: 583 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3232 4133 4395 820
Size: 197 926 1289 901 262 1553
Fragments arranged by size:
1553 1289 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2919 493
Size: 2426 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5128
SphI G_CATG'C
Cuts at: 1132 2486 1132
Size: 1354 3774
SspI AAT'ATT
Cuts at: 5 52 3052 5
Size: 47 3000 2081
Fragments arranged by size:
3000 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5128
StyI C'CwwG_G
Cuts at: 513 1065 2004 2567 513
Size: 552 939 563 3074
Fragments arranged by size:
3074 939 563 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3155 3172 3325 3510 3955 325
Size: 1255 417 1158 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1158 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 3374 5086
Size: 274 80 33 51 1443 54 1343 1712
Cuts at: 5086 96
Size: 138
Fragments arranged by size:
1712 1443 1343 274 138 80 54 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2349 2381 5088 5120 2349
Size: 32 2707 32 2357
Fragments arranged by size:
2707 2357 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5128
XbaI T'CTAG_A
Cuts at: 2464 2464
Size: 5128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 2290
Size: 5128
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5128
XmaI C'CCGG_G
Cuts at: 1870 2453 1870
Size: 583 4545
XmnI GAAnn'nnTTC
Cuts at: 3257 3257
Size: 5128
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2936 276
Size: 53 83 186 936 1402 2468
Fragments arranged by size:
2468 1402 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI