Restriction map for
pFN21AE6163
(Circular) (Six-base) MAPSORT of: pFN21AE6163.seq Check: 2943 from: 1 to: 4870
pFN21AE6163 4870 bp
With 182 enzymes: SgfI *
December 8, 2014 13:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2680 278
Size: 53 83 186 936 1144 2468
Fragments arranged by size:
2468 1144 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2213 1937
Size: 276 4594
Acc65I G'GTAC_C
Cuts at: 2191 2191
Size: 4870
AclI AA'CG_TT
Cuts at: 1563 2997 3370 1563
Size: 1434 373 3063
Fragments arranged by size:
3063 1434 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2933 4317 4585 1632
Size: 1301 1384 268 1917
Fragments arranged by size:
1917 1384 1301 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4673
AflIII A'CryG_T
Cuts at: 1203 3769 4746 1203
Size: 2566 977 1327
Fragments arranged by size:
2566 1327 977
AgeI A'CCGG_T
Cuts at: 3701 3701
Size: 4870
AhdI GACnn_n'nnGTC
Cuts at: 3599 3599
Size: 4870
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4870
AlwNI CAG_nnn'CTG
Cuts at: 2613 4185 2613
Size: 1572 3298
ApaLI G'TGCA_C
Cuts at: 2927 4083 2927
Size: 1156 3714
ApoI r'AATT_y
Cuts at: 1493 2179 2405 2557 4666 1493
Size: 686 226 152 2109 1697
Fragments arranged by size:
2109 1697 686 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3424 160
Size: 872 2392 1606
Fragments arranged by size:
2392 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4870
AvaI C'yCGr_G
Cuts at: 1870 1943 2195 1870
Size: 73 252 4545
Fragments arranged by size:
4545 252 73
BamHI G'GATC_C
Cuts at: 2200 2567 2200
Size: 367 4503
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 3646 618
Size: 325 217 627 404 1455 1842
Fragments arranged by size:
1842 1455 627 404 325 217
BanII G_rGCy'C
Cuts at: 1391 2189 1391
Size: 798 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3061 3095 1903
Size: 34 1124 34 3678
Fragments arranged by size:
3678 1124 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2762 3972 2762
Size: 1210 3660
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4870
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3481 136
Size: 107 122 71 631 605 1809 1525
Fragments arranged by size:
1809 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4865 4865
Size: 4870
BlpI GC'TnA_GC
Cuts at: 2015 2287 2015
Size: 272 4598
Bme1580I G_kGCm'C
Cuts at: 1790 2931 4087 1790
Size: 1141 1156 2573
Fragments arranged by size:
2573 1156 1141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4870
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2601 3559 442
Size: 416 53 83 399 24 1184 958 1753
Fragments arranged by size:
1753 1184 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3530 1239
Size: 2291 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2995 3860 4158 2995
Size: 865 298 3707
Fragments arranged by size:
3707 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3533 882
Size: 617 2034 2219
Fragments arranged by size:
2219 2034 617
BsaAI yAC'GTr
Cuts at: 493 1204 2661 493
Size: 711 1457 2702
Fragments arranged by size:
2702 1457 711
BsaBI GATnn'nnATC
Cuts at: 4864 4864
Size: 4870
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2677 3059 3694 275
Size: 53 83 186 936 1144 382 635 1451
Fragments arranged by size:
1451 1144 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3303 3701 3975 4122 4533 3303
Size: 398 274 147 411 3640
Fragments arranged by size:
3640 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4645
Fragments arranged by size:
4645 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4870
BseYI C'CCAG_C
Cuts at: 2076 4073 4569 2076
Size: 1997 496 2377
Fragments arranged by size:
2377 1997 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2142 1163
Size: 573 406 3891
Fragments arranged by size:
3891 573 406
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2320 3081 4109 1540
Size: 275 186 319 761 1028 2301
Fragments arranged by size:
2301 1028 761 319 275 186
BsiHKAI G_wGCw'C
Cuts at: 2931 3016 4087 2931
Size: 85 1071 3714
Fragments arranged by size:
3714 1071 85
BsmI GAATG_Cn'
Cuts at: 2139 2381 2474 2139
Size: 242 93 4535
Fragments arranged by size:
4535 242 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 2931 3016 4087 1391
Size: 399 399 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 399 85
BspEI T'CCGG_A
Cuts at: 4533 4533
Size: 4870
BspHI T'CATG_A
Cuts at: 2759 4489 2759
Size: 1730 3140
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2225 844
Size: 1381 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2757 1394
Size: 429 934 3507
Fragments arranged by size:
3507 934 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3365 3539 65
Size: 1183 2117 174 1396
Fragments arranged by size:
2117 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3514 3701 1700
Size: 110 1704 187 2869
Fragments arranged by size:
2869 1704 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4870
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4870
BssSI C'ACGA_G
Cuts at: 2930 3942 2930
Size: 1012 3858
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4870
BstBI TT'CG_AA
Cuts at: 3674 3674
Size: 4870
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4870
BstYI r'GATC_y
Cuts at: 1972 2125 2200 2567 2952 2969 4421 4507 4519
Size: 153 75 367 385 17 1452 86 12
Cuts at: 4519 4865 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 153 86 75
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3379
Fragments arranged by size:
3379 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4131
Fragments arranged by size:
4131 683 56
BtsI GCAGTG_nn'
Cuts at: 2386 3180 3200 2386
Size: 794 20 4056
Fragments arranged by size:
4056 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4870
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4835
DraI TTT'AAA
Cuts at: 2174 2174
Size: 4870
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3877 809
Size: 507 2561 1802
Fragments arranged by size:
2561 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2317 3206 4835
Size: 54 1425 50 275 18 487 889 1629
Cuts at: 4835 8
Size: 43
Fragments arranged by size:
1629 1425 889 487 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2317 1537
Size: 275 505 4090
Fragments arranged by size:
4090 505 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2800 1353
Size: 701 746 3423
Fragments arranged by size:
3423 746 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3457 3831 3977 387
Size: 1064 138 1868 374 146 1280
Fragments arranged by size:
1868 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2933 3530 4317 4585 1239
Size: 393 1301 597 787 268 1524
Fragments arranged by size:
1524 1301 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2179 2557 2179
Size: 378 4492
FspI TGC'GCA
Cuts at: 3376 3376
Size: 4870
HaeII r_GCGC'y
Cuts at: 1678 4017 1678
Size: 2339 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3511 3543 3585 3617 1373
Size: 32 135 32 1939 32 42 32 2626
Fragments arranged by size:
2626 1939 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2214 2460 669
Size: 988 281 276 246 3079
Fragments arranged by size:
3079 988 281 276 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4870
HpaI GTT'AAC
Cuts at: 2460 2460
Size: 4870
KpnI G_GTAC'C
Cuts at: 2195 2195
Size: 4870
MfeI C'AATT_G
Cuts at: 2469 2469
Size: 4870
MluI A'CGCG_T
Cuts at: 4746 4746
Size: 4870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3984 4168 1305
Size: 2679 184 2007
Fragments arranged by size:
2679 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3048
Fragments arranged by size:
3048 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2828 3187 3346 518
Size: 648 1662 359 159 2042
Fragments arranged by size:
2042 1662 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2286 2963 4111 4356 1606
Size: 680 677 1148 245 2120
Fragments arranged by size:
2120 1148 680 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3379
Fragments arranged by size:
3379 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4870
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4870
NotI GC'GGCC_GC
Cuts at: 2317 2317
Size: 4870
NspI r_CATG'y
Cuts at: 1132 1229 2228 3773 1132
Size: 97 999 1545 2229
Fragments arranged by size:
2229 1545 999 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4870
PciI A'CATG_T
Cuts at: 3769 3769
Size: 4870
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2076 1108
Size: 968 3902
PfoI T'CCnGG_A
Cuts at: 2128 2128
Size: 4870
PmeI GTTT'AAAC
Cuts at: 2174 2174
Size: 4870
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 4452 4484 2939
Size: 32 1481 32 3325
Fragments arranged by size:
3325 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4870
PsiI TTA'TAA
Cuts at: 2440 2440
Size: 4870
PstI C_TGCA'G
Cuts at: 830 2222 830
Size: 1392 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4870
SalI G'TCGA_C
Cuts at: 1936 2212 1936
Size: 276 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 4870
SbfI CC_TGCA'GG
Cuts at: 2222 2222
Size: 4870
ScaI AGT'ACT
Cuts at: 3118 4830 3118
Size: 1712 3158
SfcI C'TryA_G
Cuts at: 826 1045 2039 2218 3353 4034 4225 826
Size: 219 994 179 1135 681 191 1471
Fragments arranged by size:
1471 1135 994 681 219 191 179
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4870
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4870
SmaI CCC'GGG
Cuts at: 1872 2197 1872
Size: 325 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2974 3875 4137 820
Size: 197 926 1031 901 262 1553
Fragments arranged by size:
1553 1031 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2661 493
Size: 2168 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4870
SphI G_CATG'C
Cuts at: 1132 2228 1132
Size: 1096 3774
SspI AAT'ATT
Cuts at: 5 52 2794 5
Size: 47 2742 2081
Fragments arranged by size:
2742 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4870
StyI C'CwwG_G
Cuts at: 513 1065 2004 2309 513
Size: 552 939 305 3074
Fragments arranged by size:
3074 939 552 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2897 2914 3067 3252 3697 325
Size: 1255 1317 17 153 185 445 1498
Fragments arranged by size:
1498 1317 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3116 4828 96
Size: 274 80 33 51 1443 1139 1712 138
Fragments arranged by size:
1712 1443 1139 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4830 4862 4830
Size: 32 4838
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4870
XbaI T'CTAG_A
Cuts at: 2206 2206
Size: 4870
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4870
XmaI C'CCGG_G
Cuts at: 1870 2195 1870
Size: 325 4545
XmnI GAAnn'nnTTC
Cuts at: 2999 2999
Size: 4870
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2678 276
Size: 53 83 186 936 1144 2468
Fragments arranged by size:
2468 1144 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI