Restriction map for
pFN21AE6160
(Circular) (Six-base) MAPSORT of: pFN21AE6160.seq Check: 112 from: 1 to: 5053
pFN21AE6160 5053 bp
With 182 enzymes: SgfI *
December 8, 2014 13:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2089 2089
Size: 5053
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2863 278
Size: 53 83 186 936 1327 2468
Fragments arranged by size:
2468 1327 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2396 1937
Size: 459 4594
Acc65I G'GTAC_C
Cuts at: 2374 2374
Size: 5053
AclI AA'CG_TT
Cuts at: 1563 3180 3553 1563
Size: 1617 373 3063
Fragments arranged by size:
3063 1617 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 3116 4500 4768 1632
Size: 734 750 1384 268 1917
Fragments arranged by size:
1917 1384 750 734 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4856
AflIII A'CryG_T
Cuts at: 1203 3952 4929 1203
Size: 2749 977 1327
Fragments arranged by size:
2749 1327 977
AgeI A'CCGG_T
Cuts at: 3884 3884
Size: 5053
AhdI GACnn_n'nnGTC
Cuts at: 3782 3782
Size: 5053
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5053
AlwNI CAG_nnn'CTG
Cuts at: 2796 4368 2796
Size: 1572 3481
ApaLI G'TGCA_C
Cuts at: 3110 4266 3110
Size: 1156 3897
ApoI r'AATT_y
Cuts at: 1493 2322 2362 2588 2740 4849 1493
Size: 829 40 226 152 2109 1697
Fragments arranged by size:
2109 1697 829 226 152 40
AseI AT'TA_AT
Cuts at: 160 1032 3607 160
Size: 872 2575 1606
Fragments arranged by size:
2575 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5053
AvaI C'yCGr_G
Cuts at: 1870 1943 2378 1870
Size: 73 435 4545
Fragments arranged by size:
4545 435 73
BamHI G'GATC_C
Cuts at: 2383 2750 2383
Size: 367 4686
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2374 3829 618
Size: 325 217 627 587 1455 1842
Fragments arranged by size:
1842 1455 627 587 325 217
BanII G_rGCy'C
Cuts at: 1391 2229 2372 1391
Size: 838 143 4072
Fragments arranged by size:
4072 838 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3244 3278 1903
Size: 34 1307 34 3678
Fragments arranged by size:
3678 1307 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2945 4155 2945
Size: 1210 3843
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5053
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3664 136
Size: 107 122 71 631 605 1992 1525
Fragments arranged by size:
1992 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2299 5048 2299
Size: 2749 2304
BlpI GC'TnA_GC
Cuts at: 2051 2312 2470 2051
Size: 261 158 4634
Fragments arranged by size:
4634 261 158
Bme1580I G_kGCm'C
Cuts at: 1790 3114 4270 1790
Size: 1324 1156 2573
Fragments arranged by size:
2573 1324 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5053
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2784 3742 442
Size: 416 53 83 399 24 1367 958 1753
Fragments arranged by size:
1753 1367 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 3713 1239
Size: 813 1661 2579
Fragments arranged by size:
2579 1661 813
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 3178 4043 4341 2213
Size: 965 865 298 2925
Fragments arranged by size:
2925 965 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3716 882
Size: 617 2217 2219
Fragments arranged by size:
2219 2217 617
BsaAI yAC'GTr
Cuts at: 493 1204 2844 493
Size: 711 1640 2702
Fragments arranged by size:
2702 1640 711
BsaBI GATnn'nnATC
Cuts at: 5047 5047
Size: 5053
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2860 3242 3877 275
Size: 53 83 186 936 1327 382 635 1451
Fragments arranged by size:
1451 1327 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3486 3884 4158 4305 4716 3486
Size: 398 274 147 411 3823
Fragments arranged by size:
3823 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4828
Fragments arranged by size:
4828 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2001 2024 2027 2112 1186
Size: 815 23 3 85 4127
Fragments arranged by size:
4127 815 85 23 3
BseYI C'CCAG_C
Cuts at: 4256 4752 4256
Size: 496 4557
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2503 3264 4292 1540
Size: 275 186 502 761 1028 2301
Fragments arranged by size:
2301 1028 761 502 275 186
BsiHKAI G_wGCw'C
Cuts at: 3114 3199 4270 3114
Size: 85 1071 3897
Fragments arranged by size:
3897 1071 85
BsmI GAATG_Cn'
Cuts at: 2564 2657 2564
Size: 93 4960
BsmBI CGTCTCn'nnnn_
Cuts at: 2267 2267
Size: 5053
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2372 3114 3199 4270 1391
Size: 399 439 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 143 85
BspEI T'CCGG_A
Cuts at: 4716 4716
Size: 5053
BspHI T'CATG_A
Cuts at: 2942 4672 2942
Size: 1730 3323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2089 2408 844
Size: 1245 319 3489
Fragments arranged by size:
3489 1245 319
BsrBI CCG'CTC
Cuts at: 1394 1823 2940 1394
Size: 429 1117 3507
Fragments arranged by size:
3507 1117 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3548 3722 65
Size: 1183 2300 174 1396
Fragments arranged by size:
2300 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3697 3884 1700
Size: 110 1887 187 2869
Fragments arranged by size:
2869 1887 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5053
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5053
BssSI C'ACGA_G
Cuts at: 3113 4125 3113
Size: 1012 4041
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5053
BstBI TT'CG_AA
Cuts at: 3857 3857
Size: 5053
BstEII G'GTnAC_C
Cuts at: 1184 2162 1184
Size: 978 4075
BstYI r'GATC_y
Cuts at: 1972 2196 2299 2383 2750 3135 3152 4604 4690
Size: 224 103 84 367 385 17 1452 86
Cuts at: 4690 4702 5048 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 224 103 86
84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3562
Fragments arranged by size:
3562 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4314
Fragments arranged by size:
4314 683 56
BtsI GCAGTG_nn'
Cuts at: 2569 3363 3383 2569
Size: 794 20 4239
Fragments arranged by size:
4239 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5053
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5018
DraI TTT'AAA
Cuts at: 2357 2357
Size: 5053
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4060 809
Size: 507 2744 1802
Fragments arranged by size:
2744 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2500 3389 5018
Size: 54 1425 50 275 18 670 889 1629
Cuts at: 5018 8
Size: 43
Fragments arranged by size:
1629 1425 889 670 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2500 1537
Size: 275 688 4090
Fragments arranged by size:
4090 688 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2983 1353
Size: 1630 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3640 4014 4160 387
Size: 1064 138 2051 374 146 1280
Fragments arranged by size:
2051 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2366 3116 3713 4500 4768 1239
Size: 393 420 314 750 597 787 268 1524
Fragments arranged by size:
1524 787 750 597 420 393 314 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2210 2257 2210
Size: 47 5006
EcoO109I rG'GnC_Cy
Cuts at: 2135 2135
Size: 5053
EcoRI G'AATT_C
Cuts at: 2362 2740 2362
Size: 378 4675
FspI TGC'GCA
Cuts at: 3559 3559
Size: 5053
HaeII r_GCGC'y
Cuts at: 1678 4200 1678
Size: 2522 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 3694 3726 3768
Size: 32 135 32 712 32 1378 32 42
Cuts at: 3768 3800 1373
Size: 32 2626
Fragments arranged by size:
2626 1378 712 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2397 2643 669
Size: 988 281 459 246 3079
Fragments arranged by size:
3079 988 459 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5053
HpaI GTT'AAC
Cuts at: 2643 2643
Size: 5053
KpnI G_GTAC'C
Cuts at: 2378 2378
Size: 5053
MfeI C'AATT_G
Cuts at: 2652 2652
Size: 5053
MluI A'CGCG_T
Cuts at: 4929 4929
Size: 5053
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4167 4351 1305
Size: 2862 184 2007
Fragments arranged by size:
2862 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3231
Fragments arranged by size:
3231 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3011 3370 3529 518
Size: 648 1845 359 159 2042
Fragments arranged by size:
2042 1845 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2469 3146 4294 4539 1606
Size: 863 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3562
Fragments arranged by size:
3562 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5053
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5053
NotI GC'GGCC_GC
Cuts at: 2500 2500
Size: 5053
NspI r_CATG'y
Cuts at: 1132 1229 2311 2411 3956 1132
Size: 97 1082 100 1545 2229
Fragments arranged by size:
2229 1545 1082 100 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5053
PciI A'CATG_T
Cuts at: 3952 3952
Size: 5053
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2294 1108
Size: 1186 3867
PmeI GTTT'AAAC
Cuts at: 2357 2357
Size: 5053
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3154 4635 4667 3122
Size: 32 1481 32 3508
Fragments arranged by size:
3508 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2135 2135
Size: 5053
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5053
PsiI TTA'TAA
Cuts at: 2623 2623
Size: 5053
PstI C_TGCA'G
Cuts at: 830 2405 830
Size: 1575 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5053
SalI G'TCGA_C
Cuts at: 1936 2395 1936
Size: 459 4594
SanDI GG'GwC_CC
Cuts at: 2135 2135
Size: 5053
SbfI CC_TGCA'GG
Cuts at: 2405 2405
Size: 5053
ScaI AGT'ACT
Cuts at: 3301 5013 3301
Size: 1712 3341
SfcI C'TryA_G
Cuts at: 826 1045 2401 3536 4217 4408 826
Size: 219 1356 1135 681 191 1471
Fragments arranged by size:
1471 1356 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5053
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5053
SmaI CCC'GGG
Cuts at: 1872 2380 1872
Size: 508 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 3157 4058 4320 820
Size: 197 926 249 965 901 262 1553
Fragments arranged by size:
1553 965 926 901 262 249 197
SnaBI TAC'GTA
Cuts at: 493 2844 493
Size: 2351 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5053
SphI G_CATG'C
Cuts at: 1132 2311 2411 1132
Size: 1179 100 3774
Fragments arranged by size:
3774 1179 100
SspI AAT'ATT
Cuts at: 5 52 2977 5
Size: 47 2925 2081
Fragments arranged by size:
2925 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5053
StyI C'CwwG_G
Cuts at: 513 1065 2004 2328 2492 513
Size: 552 939 324 164 3074
Fragments arranged by size:
3074 939 552 324 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 3080 3097 3250 3435 3880 325
Size: 1255 570 930 17 153 185 445 1498
Fragments arranged by size:
1498 1255 930 570 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3299 5011 96
Size: 274 80 33 51 1443 1322 1712 138
Fragments arranged by size:
1712 1443 1322 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5013 5045 5013
Size: 32 5021
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5053
XbaI T'CTAG_A
Cuts at: 2389 2389
Size: 5053
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5053
XmaI C'CCGG_G
Cuts at: 1870 2378 1870
Size: 508 4545
XmnI GAAnn'nnTTC
Cuts at: 3182 3182
Size: 5053
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2861 276
Size: 53 83 186 936 1327 2468
Fragments arranged by size:
2468 1327 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI