Restriction map for
pFN21AE6159
(Circular) (Six-base) MAPSORT of: pFN21AE6159.seq Check: 327 from: 1 to: 5068
pFN21AE6159 5068 bp
With 182 enzymes: SgfI *
December 8, 2014 13:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2878 278
Size: 53 83 186 936 1342 2468
Fragments arranged by size:
2468 1342 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2411 1937
Size: 474 4594
Acc65I G'GTAC_C
Cuts at: 2389 2389
Size: 5068
AclI AA'CG_TT
Cuts at: 1563 3195 3568 1563
Size: 1632 373 3063
Fragments arranged by size:
3063 1632 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3131 4515 4783 1632
Size: 1499 1384 268 1917
Fragments arranged by size:
1917 1499 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4871
AflIII A'CryG_T
Cuts at: 1203 3967 4944 1203
Size: 2764 977 1327
Fragments arranged by size:
2764 1327 977
AgeI A'CCGG_T
Cuts at: 3899 3899
Size: 5068
AhdI GACnn_n'nnGTC
Cuts at: 3797 3797
Size: 5068
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5068
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5068
AlwNI CAG_nnn'CTG
Cuts at: 2149 2161 2811 4383 2149
Size: 12 650 1572 2834
Fragments arranged by size:
2834 1572 650 12
ApaI G_GGCC'C
Cuts at: 2080 2080
Size: 5068
ApaLI G'TGCA_C
Cuts at: 3125 4281 3125
Size: 1156 3912
ApoI r'AATT_y
Cuts at: 1493 2377 2603 2755 4864 1493
Size: 884 226 152 2109 1697
Fragments arranged by size:
2109 1697 884 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3622 160
Size: 872 2590 1606
Fragments arranged by size:
2590 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5068
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2393 1870
Size: 73 99 351 4545
Fragments arranged by size:
4545 351 99 73
BamHI G'GATC_C
Cuts at: 2398 2765 2398
Size: 367 4701
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2389 3844 618
Size: 325 217 627 602 1455 1842
Fragments arranged by size:
1842 1455 627 602 325 217
BanII G_rGCy'C
Cuts at: 1391 2051 2080 2115 2387 1391
Size: 660 29 35 272 4072
Fragments arranged by size:
4072 660 272 35 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4097
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3259 3293 1903
Size: 34 1322 34 3678
Fragments arranged by size:
3678 1322 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2960 4170 2960
Size: 1210 3858
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5068
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2311 3679 136
Size: 107 122 71 631 605 639 1368 1525
Fragments arranged by size:
1525 1368 639 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5063 5063
Size: 5068
BlpI GC'TnA_GC
Cuts at: 2114 2216 2485 2114
Size: 102 269 4697
Fragments arranged by size:
4697 269 102
Bme1580I G_kGCm'C
Cuts at: 1790 2080 3129 4285 1790
Size: 290 1049 1156 2573
Fragments arranged by size:
2573 1156 1049 290
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5068
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2799 3757 442
Size: 416 53 83 399 24 1382 958 1753
Fragments arranged by size:
1753 1382 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5068
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 3728 1239
Size: 887 1602 2579
Fragments arranged by size:
2579 1602 887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3193 4058 4356 3193
Size: 865 298 3905
Fragments arranged by size:
3905 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2298 3731 882
Size: 617 799 1433 2219
Fragments arranged by size:
2219 1433 799 617
BsaAI yAC'GTr
Cuts at: 493 1204 2859 493
Size: 711 1655 2702
Fragments arranged by size:
2702 1655 711
BsaBI GATnn'nnATC
Cuts at: 5062 5062
Size: 5068
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2875 3257 3892 275
Size: 53 83 186 936 1342 382 635 1451
Fragments arranged by size:
1451 1342 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3501 3899 4173 4320 4731 3501
Size: 398 274 147 411 3838
Fragments arranged by size:
3838 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2278 2308 1178
Size: 30 165 30 875 30 3938
Fragments arranged by size:
3938 875 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 1186
Size: 844 4224
BseYI C'CCAG_C
Cuts at: 4271 4767 4271
Size: 496 4572
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4495
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2518 3279 4307 1540
Size: 275 186 517 761 1028 2301
Fragments arranged by size:
2301 1028 761 517 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 3129 3214 4285 2115
Size: 1014 85 1071 2898
Fragments arranged by size:
2898 1071 1014 85
BsmI GAATG_Cn'
Cuts at: 2579 2672 2579
Size: 93 4975
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2080 2115 2387 3129 3214 4285
Size: 399 261 29 35 272 742 85 1071
Cuts at: 4285 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 272 261 85 35
29
BspEI T'CCGG_A
Cuts at: 4731 4731
Size: 5068
BspHI T'CATG_A
Cuts at: 2957 4687 2957
Size: 1730 3338
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2423 844
Size: 1579 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2955 1394
Size: 429 1132 3507
Fragments arranged by size:
3507 1132 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3563 3737 65
Size: 1183 2315 174 1396
Fragments arranged by size:
2315 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3712 3899 1700
Size: 110 1902 187 2869
Fragments arranged by size:
2869 1902 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5068
BssHII G'CGCG_C
Cuts at: 1925 2057 2133 1925
Size: 132 76 4860
Fragments arranged by size:
4860 132 76
BssSI C'ACGA_G
Cuts at: 3128 4140 3128
Size: 1012 4056
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5068
BstBI TT'CG_AA
Cuts at: 3872 3872
Size: 5068
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5068
BstYI r'GATC_y
Cuts at: 1972 2398 2765 3150 3167 4619 4705 4717 5063
Size: 426 367 385 17 1452 86 12 346
Cuts at: 5063 1972
Size: 1977
Fragments arranged by size:
1977 1452 426 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3577
Fragments arranged by size:
3577 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4329
Fragments arranged by size:
4329 683 56
BtsI GCAGTG_nn'
Cuts at: 2584 3378 3398 2584
Size: 794 20 4254
Fragments arranged by size:
4254 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5068
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5033
DraI TTT'AAA
Cuts at: 2372 2372
Size: 5068
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4075 809
Size: 507 2759 1802
Fragments arranged by size:
2759 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2515 3404 5033
Size: 54 1425 50 275 18 685 889 1629
Cuts at: 5033 8
Size: 43
Fragments arranged by size:
1629 1425 889 685 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2515 1537
Size: 275 703 4090
Fragments arranged by size:
4090 703 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2998 1353
Size: 1645 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2041 3655 4029 4175 387
Size: 1064 138 452 1614 374 146 1280
Fragments arranged by size:
1614 1280 1064 452 374 146 138
EcoICRI GAG'CTC
Cuts at: 2113 2113
Size: 5068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 3131 3728 4515 4783 1239
Size: 393 494 1005 597 787 268 1524
Fragments arranged by size:
1524 1005 787 597 494 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2126 2209 2126
Size: 83 4985
EcoO109I rG'GnC_Cy
Cuts at: 2076 2158 2286 2076
Size: 82 128 4858
Fragments arranged by size:
4858 128 82
EcoRI G'AATT_C
Cuts at: 2377 2755 2377
Size: 378 4690
FspI TGC'GCA
Cuts at: 3574 3574
Size: 5068
HaeII r_GCGC'y
Cuts at: 1678 2340 4215 1678
Size: 662 1875 2531
Fragments arranged by size:
2531 1875 662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2278 2310 3709 3741 3783
Size: 32 135 32 706 32 1399 32 42
Cuts at: 3783 3815 1373
Size: 32 2626
Fragments arranged by size:
2626 1399 706 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2412 2658 669
Size: 988 281 474 246 3079
Fragments arranged by size:
3079 988 474 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5068
HpaI GTT'AAC
Cuts at: 2658 2658
Size: 5068
KpnI G_GTAC'C
Cuts at: 2393 2393
Size: 5068
MfeI C'AATT_G
Cuts at: 2667 2667
Size: 5068
MluI A'CGCG_T
Cuts at: 4944 4944
Size: 5068
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2271 4182 4366 1305
Size: 966 1911 184 2007
Fragments arranged by size:
2007 1911 966 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3246
Fragments arranged by size:
3246 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3026 3385 3544 518
Size: 648 1860 359 159 2042
Fragments arranged by size:
2042 1860 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2333 2484 3161 4309 4554 1606
Size: 540 187 151 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 245 187 151
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3577
Fragments arranged by size:
3577 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5068
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5068
NotI GC'GGCC_GC
Cuts at: 2515 2515
Size: 5068
NspI r_CATG'y
Cuts at: 1132 1229 2254 2426 3971 1132
Size: 97 1025 172 1545 2229
Fragments arranged by size:
2229 1545 1025 172 97
PasI CC'CwG_GG
Cuts at: 1348 2073 2309 1348
Size: 725 236 4107
Fragments arranged by size:
4107 725 236
PciI A'CATG_T
Cuts at: 3967 3967
Size: 5068
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5068
PmeI GTTT'AAAC
Cuts at: 2372 2372
Size: 5068
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 4650 4682 3137
Size: 32 1481 32 3523
Fragments arranged by size:
3523 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2286 2286
Size: 5068
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5068
PsiI TTA'TAA
Cuts at: 2638 2638
Size: 5068
PspOMI G'GGCC_C
Cuts at: 2076 2076
Size: 5068
PstI C_TGCA'G
Cuts at: 830 2265 2347 2420 830
Size: 1435 82 73 3478
Fragments arranged by size:
3478 1435 82 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5068
PvuII CAG'CTG
Cuts at: 2333 2333
Size: 5068
SacI G_AGCT'C
Cuts at: 2115 2115
Size: 5068
SalI G'TCGA_C
Cuts at: 1936 2410 1936
Size: 474 4594
SbfI CC_TGCA'GG
Cuts at: 2265 2420 2265
Size: 155 4913
ScaI AGT'ACT
Cuts at: 3316 5028 3316
Size: 1712 3356
SexAI A'CCwGG_T
Cuts at: 2288 2288
Size: 5068
SfcI C'TryA_G
Cuts at: 826 1045 2153 2261 2343 2416 3551 4232 4423
Size: 219 1108 108 82 73 1135 681 191
Cuts at: 4423 826
Size: 1471
Fragments arranged by size:
1471 1135 1108 681 219 191 108 82
73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5068
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5068
SmaI CCC'GGG
Cuts at: 1872 2395 1872
Size: 523 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3172 4073 4335 820
Size: 197 926 1229 901 262 1553
Fragments arranged by size:
1553 1229 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2859 493
Size: 2366 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5068
SphI G_CATG'C
Cuts at: 1132 2426 1132
Size: 1294 3774
SspI AAT'ATT
Cuts at: 5 52 2992 5
Size: 47 2940 2081
Fragments arranged by size:
2940 2081 47
StuI AGG'CCT
Cuts at: 1517 2267 1517
Size: 750 4318
StyI C'CwwG_G
Cuts at: 513 1065 2004 2507 513
Size: 552 939 503 3074
Fragments arranged by size:
3074 939 552 503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3095 3112 3265 3450 3895 325
Size: 1255 1515 17 153 185 445 1498
Fragments arranged by size:
1515 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3314 5026 96
Size: 274 80 33 51 1443 1337 1712 138
Fragments arranged by size:
1712 1443 1337 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5028 5060 5028
Size: 32 5036
Tth111I GACn'n_nGTC
Cuts at: 1316 2290 1316
Size: 974 4094
XbaI T'CTAG_A
Cuts at: 2404 2404
Size: 5068
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5068
XmaI C'CCGG_G
Cuts at: 1870 2393 1870
Size: 523 4545
XmnI GAAnn'nnTTC
Cuts at: 3197 3197
Size: 5068
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2876 276
Size: 53 83 186 936 1342 2468
Fragments arranged by size:
2468 1342 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI