Restriction map for
pFN21AE6154
(Circular) (Six-base) MAPSORT of: pFN21AE6154.seq Check: 1073 from: 1 to: 4939
pFN21AE6154 4939 bp
With 182 enzymes: SgfI *
December 8, 2014 13:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2749 278
Size: 53 83 186 936 1213 2468
Fragments arranged by size:
2468 1213 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2282 1937
Size: 345 4594
Acc65I G'GTAC_C
Cuts at: 2260 2260
Size: 4939
AclI AA'CG_TT
Cuts at: 1563 3066 3439 1563
Size: 1503 373 3063
Fragments arranged by size:
3063 1503 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 3002 4386 4654 1632
Size: 456 914 1384 268 1917
Fragments arranged by size:
1917 1384 914 456 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4742
AflIII A'CryG_T
Cuts at: 1203 3838 4815 1203
Size: 2635 977 1327
Fragments arranged by size:
2635 1327 977
AgeI A'CCGG_T
Cuts at: 3770 3770
Size: 4939
AhdI GACnn_n'nnGTC
Cuts at: 3668 3668
Size: 4939
AleI CACnn'nnGTG
Cuts at: 1166 2075 1166
Size: 909 4030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4939
AlwNI CAG_nnn'CTG
Cuts at: 2682 4254 2682
Size: 1572 3367
ApaLI G'TGCA_C
Cuts at: 2996 4152 2996
Size: 1156 3783
ApoI r'AATT_y
Cuts at: 1493 2248 2474 2626 4735 1493
Size: 755 226 152 2109 1697
Fragments arranged by size:
2109 1697 755 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2154 3493 160
Size: 872 1122 1339 1606
Fragments arranged by size:
1606 1339 1122 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4939
AvaI C'yCGr_G
Cuts at: 1870 1943 2264 1870
Size: 73 321 4545
Fragments arranged by size:
4545 321 73
BamHI G'GATC_C
Cuts at: 2269 2636 2269
Size: 367 4572
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2260 3715 618
Size: 325 217 627 473 1455 1842
Fragments arranged by size:
1842 1455 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 2258 1391
Size: 867 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3968
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3130 3164 1903
Size: 34 1193 34 3678
Fragments arranged by size:
3678 1193 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2831 4041 2831
Size: 1210 3729
BclI T'GATC_A
Cuts at: 1548 2211 1548
Size: 663 4276
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3550 136
Size: 107 122 71 631 605 1878 1525
Fragments arranged by size:
1878 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4934 4934
Size: 4939
BlpI GC'TnA_GC
Cuts at: 2356 2356
Size: 4939
Bme1580I G_kGCm'C
Cuts at: 1790 3000 4156 1790
Size: 1210 1156 2573
Fragments arranged by size:
2573 1210 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4939
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2670 3628 442
Size: 416 53 83 399 24 1253 958 1753
Fragments arranged by size:
1753 1253 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3599 1239
Size: 2360 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3064 3929 4227 3064
Size: 865 298 3776
Fragments arranged by size:
3776 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3602 882
Size: 617 2103 2219
Fragments arranged by size:
2219 2103 617
BsaAI yAC'GTr
Cuts at: 493 1204 2730 493
Size: 711 1526 2702
Fragments arranged by size:
2702 1526 711
BsaBI GATnn'nnATC
Cuts at: 4933 4933
Size: 4939
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2746 3128 3763 275
Size: 53 83 186 936 1213 382 635 1451
Fragments arranged by size:
1451 1213 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3372 3770 4044 4191 4602 3372
Size: 398 274 147 411 3709
Fragments arranged by size:
3709 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4714
Fragments arranged by size:
4714 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4939
BseYI C'CCAG_C
Cuts at: 4142 4638 4142
Size: 496 4443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4366
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2389 3150 4178 1540
Size: 275 186 388 761 1028 2301
Fragments arranged by size:
2301 1028 761 388 275 186
BsiHKAI G_wGCw'C
Cuts at: 3000 3085 4156 3000
Size: 85 1071 3783
Fragments arranged by size:
3783 1071 85
BsmI GAATG_Cn'
Cuts at: 2450 2543 2450
Size: 93 4846
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2258 3000 3085 4156 1391
Size: 399 468 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 468 399 85
BspEI T'CCGG_A
Cuts at: 4602 4602
Size: 4939
BspHI T'CATG_A
Cuts at: 2828 4558 2828
Size: 1730 3209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2294 844
Size: 1450 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2826 1394
Size: 429 1003 3507
Fragments arranged by size:
3507 1003 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 3434 3608 65
Size: 1183 840 1346 174 1396
Fragments arranged by size:
1396 1346 1183 840 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3583 3770 1700
Size: 110 1773 187 2869
Fragments arranged by size:
2869 1773 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4939
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4939
BssSI C'ACGA_G
Cuts at: 2172 2999 4011 2172
Size: 827 1012 3100
Fragments arranged by size:
3100 1012 827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4939
BstBI TT'CG_AA
Cuts at: 3743 3743
Size: 4939
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4939
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2077
Size: 4939
BstYI r'GATC_y
Cuts at: 1972 2269 2636 3021 3038 4490 4576 4588 4934
Size: 297 367 385 17 1452 86 12 346
Cuts at: 4934 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 297 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2180 487
Size: 683 56 954 3246
Fragments arranged by size:
3246 954 683 56
BtsI GCAGTG_nn'
Cuts at: 2455 3249 3269 2455
Size: 794 20 4125
Fragments arranged by size:
4125 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4904
DraI TTT'AAA
Cuts at: 2243 2243
Size: 4939
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3946 809
Size: 507 2630 1802
Fragments arranged by size:
2630 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2386 3275 4904
Size: 54 1425 50 275 18 556 889 1629
Cuts at: 4904 8
Size: 43
Fragments arranged by size:
1629 1425 889 556 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2386 1537
Size: 275 574 4090
Fragments arranged by size:
4090 574 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2104 2869 1353
Size: 751 765 3423
Fragments arranged by size:
3423 765 751
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3526 3900 4046 387
Size: 1064 138 1937 374 146 1280
Fragments arranged by size:
1937 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 3002 3599 4386 4654 1239
Size: 393 456 914 597 787 268 1524
Fragments arranged by size:
1524 914 787 597 456 393 268
EcoRI G'AATT_C
Cuts at: 2248 2626 2248
Size: 378 4561
FspI TGC'GCA
Cuts at: 3445 3445
Size: 4939
HaeII r_GCGC'y
Cuts at: 1678 4086 1678
Size: 2408 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3580 3612 3654 3686 1373
Size: 32 135 32 2008 32 42 32 2626
Fragments arranged by size:
2626 2008 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2283 2529 669
Size: 988 281 345 246 3079
Fragments arranged by size:
3079 988 345 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4939
HpaI GTT'AAC
Cuts at: 2529 2529
Size: 4939
KpnI G_GTAC'C
Cuts at: 2264 2264
Size: 4939
MfeI C'AATT_G
Cuts at: 2538 2538
Size: 4939
MluI A'CGCG_T
Cuts at: 4815 4815
Size: 4939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2185 4053 4237 1305
Size: 880 1868 184 2007
Fragments arranged by size:
2007 1868 880 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3117
Fragments arranged by size:
3117 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2897 3256 3415 518
Size: 648 909 822 359 159 2042
Fragments arranged by size:
2042 909 822 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2355 3032 4180 4425 1606
Size: 749 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4387
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4939
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4939
NotI GC'GGCC_GC
Cuts at: 2386 2386
Size: 4939
NspI r_CATG'y
Cuts at: 1132 1229 2297 3842 1132
Size: 97 1068 1545 2229
Fragments arranged by size:
2229 1545 1068 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4939
PciI A'CATG_T
Cuts at: 3838 3838
Size: 4939
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4939
PmeI GTTT'AAAC
Cuts at: 2243 2243
Size: 4939
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 4521 4553 3008
Size: 32 1481 32 3394
Fragments arranged by size:
3394 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4939
PsiI TTA'TAA
Cuts at: 2509 2509
Size: 4939
PstI C_TGCA'G
Cuts at: 830 2291 830
Size: 1461 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4939
SalI G'TCGA_C
Cuts at: 1936 2281 1936
Size: 345 4594
SbfI CC_TGCA'GG
Cuts at: 2291 2291
Size: 4939
ScaI AGT'ACT
Cuts at: 3187 4899 3187
Size: 1712 3227
SfcI C'TryA_G
Cuts at: 826 1045 2287 3422 4103 4294 826
Size: 219 1242 1135 681 191 1471
Fragments arranged by size:
1471 1242 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4939
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4939
SmaI CCC'GGG
Cuts at: 1872 2266 1872
Size: 394 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3043 3944 4206 820
Size: 197 926 1100 901 262 1553
Fragments arranged by size:
1553 1100 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2730 493
Size: 2237 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4939
SphI G_CATG'C
Cuts at: 1132 2297 1132
Size: 1165 3774
SspI AAT'ATT
Cuts at: 5 52 2863 5
Size: 47 2811 2081
Fragments arranged by size:
2811 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4939
StyI C'CwwG_G
Cuts at: 513 1065 2378 513
Size: 552 1313 3074
Fragments arranged by size:
3074 1313 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2966 2983 3136 3321 3766 325
Size: 1255 1386 17 153 185 445 1498
Fragments arranged by size:
1498 1386 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3185 4897 96
Size: 274 80 33 51 1443 1208 1712 138
Fragments arranged by size:
1712 1443 1208 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4899 4931 4899
Size: 32 4907
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4939
XbaI T'CTAG_A
Cuts at: 2275 2275
Size: 4939
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4939
XmaI C'CCGG_G
Cuts at: 1870 2264 1870
Size: 394 4545
XmnI GAAnn'nnTTC
Cuts at: 3068 3068
Size: 4939
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2747 276
Size: 53 83 186 936 1213 2468
Fragments arranged by size:
2468 1213 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI