Restriction map for
pFN21AE6148
(Circular) (Six-base) MAPSORT of: pFN21AE6148.seq Check: 8922 from: 1 to: 5011
pFN21AE6148 5011 bp
With 182 enzymes: SgfI *
December 8, 2014 13:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2158 2158
Size: 5011
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2821 278
Size: 53 83 186 936 1285 2468
Fragments arranged by size:
2468 1285 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2354 1937
Size: 417 4594
Acc65I G'GTAC_C
Cuts at: 2332 2332
Size: 5011
AclI AA'CG_TT
Cuts at: 1563 3138 3511 1563
Size: 1575 373 3063
Fragments arranged by size:
3063 1575 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2291 3074 4458 4726 1632
Size: 492 167 783 1384 268 1917
Fragments arranged by size:
1917 1384 783 492 268 167
AflII C'TTAA_G
Cuts at: 820 1017 2015 820
Size: 197 998 3816
Fragments arranged by size:
3816 998 197
AflIII A'CryG_T
Cuts at: 1203 3910 4887 1203
Size: 2707 977 1327
Fragments arranged by size:
2707 1327 977
AgeI A'CCGG_T
Cuts at: 3842 3842
Size: 5011
AhdI GACnn_n'nnGTC
Cuts at: 3740 3740
Size: 5011
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5011
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5011
AlwNI CAG_nnn'CTG
Cuts at: 2754 4326 2754
Size: 1572 3439
ApaLI G'TGCA_C
Cuts at: 3068 4224 3068
Size: 1156 3855
ApoI r'AATT_y
Cuts at: 1493 2320 2546 2698 4807 1493
Size: 827 226 152 2109 1697
Fragments arranged by size:
2109 1697 827 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3565 160
Size: 872 2533 1606
Fragments arranged by size:
2533 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5011
AvaI C'yCGr_G
Cuts at: 1870 1943 2287 2336 1870
Size: 73 344 49 4545
Fragments arranged by size:
4545 344 73 49
BamHI G'GATC_C
Cuts at: 2341 2708 2341
Size: 367 4644
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2332 3787 618
Size: 325 217 627 545 1455 1842
Fragments arranged by size:
1842 1455 627 545 325 217
BanII G_rGCy'C
Cuts at: 1391 2184 2286 2330 1391
Size: 793 102 44 4072
Fragments arranged by size:
4072 793 102 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2074 2115 928
Size: 971 175 41 3824
Fragments arranged by size:
3824 971 175 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2195 2229 3202 3236 1903
Size: 34 258 34 973 34 3678
Fragments arranged by size:
3678 973 258 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2903 4113 2903
Size: 1210 3801
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5011
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3622 136
Size: 107 122 71 631 605 1950 1525
Fragments arranged by size:
1950 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5006 5006
Size: 5011
BlpI GC'TnA_GC
Cuts at: 2428 2428
Size: 5011
Bme1580I G_kGCm'C
Cuts at: 1790 3072 4228 1790
Size: 1282 1156 2573
Fragments arranged by size:
2573 1282 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5011
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2742 3700 442
Size: 416 53 83 399 24 1325 958 1753
Fragments arranged by size:
1753 1325 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5011
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3671 1239
Size: 2432 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3136 4001 4299 3136
Size: 865 298 3848
Fragments arranged by size:
3848 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3674 882
Size: 617 2175 2219
Fragments arranged by size:
2219 2175 617
BsaAI yAC'GTr
Cuts at: 493 1204 2802 493
Size: 711 1598 2702
Fragments arranged by size:
2702 1598 711
BsaBI GATnn'nnATC
Cuts at: 5005 5005
Size: 5011
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2818 3200 3835 275
Size: 53 83 186 936 1285 382 635 1451
Fragments arranged by size:
1451 1285 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3444 3842 4116 4263 4674 3444
Size: 398 274 147 411 3781
Fragments arranged by size:
3781 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4786
Fragments arranged by size:
4786 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 1186
Size: 1110 3901
BseYI C'CCAG_C
Cuts at: 4214 4710 4214
Size: 496 4515
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4438
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2461 3222 4250 1540
Size: 275 186 460 761 1028 2301
Fragments arranged by size:
2301 1028 761 460 275 186
BsiHKAI G_wGCw'C
Cuts at: 2286 3072 3157 4228 2286
Size: 786 85 1071 3069
Fragments arranged by size:
3069 1071 786 85
BsmI GAATG_Cn'
Cuts at: 2522 2615 2522
Size: 93 4918
BsmBI CGTCTCn'nnnn_
Cuts at: 2104 2104
Size: 5011
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2286 2330 3072 3157 4228 1391
Size: 399 394 102 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 102 85 44
BspEI T'CCGG_A
Cuts at: 4674 4674
Size: 5011
BspHI T'CATG_A
Cuts at: 2278 2900 4630 2278
Size: 622 1730 2659
Fragments arranged by size:
2659 1730 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2068 2158 2366 844
Size: 1224 90 208 3489
Fragments arranged by size:
3489 1224 208 90
BsrBI CCG'CTC
Cuts at: 1394 1823 2898 1394
Size: 429 1075 3507
Fragments arranged by size:
3507 1075 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3506 3680 65
Size: 1183 2258 174 1396
Fragments arranged by size:
2258 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3655 3842 1700
Size: 110 1845 187 2869
Fragments arranged by size:
2869 1845 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5011
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5011
BssSI C'ACGA_G
Cuts at: 3071 4083 3071
Size: 1012 3999
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5011
BstBI TT'CG_AA
Cuts at: 3815 3815
Size: 5011
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5011
BstYI r'GATC_y
Cuts at: 1972 2029 2341 2708 3093 3110 4562 4648 4660
Size: 57 312 367 385 17 1452 86 12
Cuts at: 4660 5006 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 312 86 57
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3520
Fragments arranged by size:
3520 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4272
Fragments arranged by size:
4272 683 56
BtsI GCAGTG_nn'
Cuts at: 2527 3321 3341 2527
Size: 794 20 4197
Fragments arranged by size:
4197 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5011
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4976
DraI TTT'AAA
Cuts at: 2315 2315
Size: 5011
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4018 809
Size: 507 2702 1802
Fragments arranged by size:
2702 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2250 2458
Size: 54 1425 50 275 18 405 15 208
Cuts at: 2458 3347 4976 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 405 275 208 54 50
43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2458 1537
Size: 275 646 4090
Fragments arranged by size:
4090 646 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2016 2941 1353
Size: 663 925 3423
Fragments arranged by size:
3423 925 663
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3598 3972 4118 387
Size: 1064 138 2009 374 146 1280
Fragments arranged by size:
2009 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2284 2284
Size: 5011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2291 3074 3671 4458 4726 1239
Size: 393 492 167 783 597 787 268 1524
Fragments arranged by size:
1524 787 783 597 492 393 268 167
EcoRI G'AATT_C
Cuts at: 2320 2698 2320
Size: 378 4633
FspI TGC'GCA
Cuts at: 3517 3517
Size: 5011
HaeII r_GCGC'y
Cuts at: 1678 4158 1678
Size: 2480 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3652 3684 3726 3758 1373
Size: 32 135 32 2080 32 42 32 2626
Fragments arranged by size:
2626 2080 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2355 2601 669
Size: 988 281 417 246 3079
Fragments arranged by size:
3079 988 417 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5011
HpaI GTT'AAC
Cuts at: 2601 2601
Size: 5011
KpnI G_GTAC'C
Cuts at: 2336 2336
Size: 5011
MfeI C'AATT_G
Cuts at: 2610 2610
Size: 5011
MluI A'CGCG_T
Cuts at: 4887 4887
Size: 5011
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4125 4309 1305
Size: 2820 184 2007
Fragments arranged by size:
2820 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 2252 10
Size: 54 1425 343 405 15 2769
Fragments arranged by size:
2769 1425 405 343 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2969 3328 3487 518
Size: 648 1803 359 159 2042
Fragments arranged by size:
2042 1803 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2427 3104 4252 4497 1606
Size: 821 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3520
Fragments arranged by size:
3520 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5011
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5011
NotI GC'GGCC_GC
Cuts at: 2458 2458
Size: 5011
NspI r_CATG'y
Cuts at: 1132 1229 2369 3914 1132
Size: 97 1140 1545 2229
Fragments arranged by size:
2229 1545 1140 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5011
PciI A'CATG_T
Cuts at: 3910 3910
Size: 5011
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5011
PmeI GTTT'AAAC
Cuts at: 2315 2315
Size: 5011
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3080 3112 4593 4625 3080
Size: 32 1481 32 3466
Fragments arranged by size:
3466 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5011
PsiI TTA'TAA
Cuts at: 2581 2581
Size: 5011
PstI C_TGCA'G
Cuts at: 830 2208 2363 830
Size: 1378 155 3478
Fragments arranged by size:
3478 1378 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5011
SacI G_AGCT'C
Cuts at: 2286 2286
Size: 5011
SalI G'TCGA_C
Cuts at: 1936 2353 1936
Size: 417 4594
SapI GCTCTTCn'nnn_
Cuts at: 2016 2016
Size: 5011
SbfI CC_TGCA'GG
Cuts at: 2363 2363
Size: 5011
ScaI AGT'ACT
Cuts at: 3259 4971 3259
Size: 1712 3299
SfcI C'TryA_G
Cuts at: 826 1045 2204 2359 3494 4175 4366 826
Size: 219 1159 155 1135 681 191 1471
Fragments arranged by size:
1471 1159 1135 681 219 191 155
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5011
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5011
SmaI CCC'GGG
Cuts at: 1872 2338 1872
Size: 466 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 3115 4016 4278 820
Size: 197 926 72 1100 901 262 1553
Fragments arranged by size:
1553 1100 926 901 262 197 72
SnaBI TAC'GTA
Cuts at: 493 2802 493
Size: 2309 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5011
SphI G_CATG'C
Cuts at: 1132 2369 1132
Size: 1237 3774
SspI AAT'ATT
Cuts at: 5 52 2935 5
Size: 47 2883 2081
Fragments arranged by size:
2883 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5011
StyI C'CwwG_G
Cuts at: 513 1065 2004 2450 513
Size: 552 939 446 3074
Fragments arranged by size:
3074 939 552 446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2310 3038 3055 3208 3393 3838 325
Size: 1255 730 728 17 153 185 445 1498
Fragments arranged by size:
1498 1255 730 728 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 3257 4969
Size: 274 80 33 51 1443 279 1001 1712
Cuts at: 4969 96
Size: 138
Fragments arranged by size:
1712 1443 1001 279 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4971 5003 4971
Size: 32 4979
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5011
XbaI T'CTAG_A
Cuts at: 2347 2347
Size: 5011
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2245 2245
Size: 5011
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5011
XmaI C'CCGG_G
Cuts at: 1870 2336 1870
Size: 466 4545
XmnI GAAnn'nnTTC
Cuts at: 2054 3140 2054
Size: 1086 3925
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2819 276
Size: 53 83 186 936 1285 2468
Fragments arranged by size:
2468 1285 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI