Restriction map for
pFN21AE6128
(Circular) (Six-base) MAPSORT of: pFN21AE6128.seq Check: 8065 from: 1 to: 5131
pFN21AE6128 5131 bp
With 182 enzymes: SgfI *
December 8, 2014 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2941 278
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 1937
Size: 537 4594
Acc65I G'GTAC_C
Cuts at: 2452 2452
Size: 5131
AclI AA'CG_TT
Cuts at: 1563 3258 3631 1563
Size: 1695 373 3063
Fragments arranged by size:
3063 1695 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3194 4578 4846 1632
Size: 1562 1384 268 1917
Fragments arranged by size:
1917 1562 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4934
AflIII A'CryG_T
Cuts at: 1203 4030 5007 1203
Size: 2827 977 1327
Fragments arranged by size:
2827 1327 977
AgeI A'CCGG_T
Cuts at: 3962 3962
Size: 5131
AhdI GACnn_n'nnGTC
Cuts at: 3860 3860
Size: 5131
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5131
AlwNI CAG_nnn'CTG
Cuts at: 2344 2874 4446 2344
Size: 530 1572 3029
Fragments arranged by size:
3029 1572 530
ApaLI G'TGCA_C
Cuts at: 3188 4344 3188
Size: 1156 3975
ApoI r'AATT_y
Cuts at: 1493 2440 2666 2818 4927 1493
Size: 947 226 152 2109 1697
Fragments arranged by size:
2109 1697 947 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3685 160
Size: 872 2653 1606
Fragments arranged by size:
2653 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
AvaI C'yCGr_G
Cuts at: 1870 1943 2456 1870
Size: 73 513 4545
Fragments arranged by size:
4545 513 73
BamHI G'GATC_C
Cuts at: 2461 2828 2461
Size: 367 4764
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2452 3907 618
Size: 325 217 627 665 1455 1842
Fragments arranged by size:
1842 1455 665 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2385 2407 2450 1391
Size: 994 22 43 4072
Fragments arranged by size:
4072 994 43 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3322 3356 1903
Size: 34 1385 34 3678
Fragments arranged by size:
3678 1385 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3023 4233 3023
Size: 1210 3921
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5131
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3742 136
Size: 107 122 71 631 605 2070 1525
Fragments arranged by size:
2070 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5126 5126
Size: 5131
BlpI GC'TnA_GC
Cuts at: 2548 2548
Size: 5131
Bme1580I G_kGCm'C
Cuts at: 1790 3192 4348 1790
Size: 1402 1156 2573
Fragments arranged by size:
2573 1402 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5131
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2038 2862 3820
Size: 416 53 83 399 24 621 824 958
Cuts at: 3820 442
Size: 1753
Fragments arranged by size:
1753 958 824 621 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 3791 1239
Size: 881 1671 2579
Fragments arranged by size:
2579 1671 881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3256 4121 4419 3256
Size: 865 298 3968
Fragments arranged by size:
3968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 3794 882
Size: 617 624 1671 2219
Fragments arranged by size:
2219 1671 624 617
BsaAI yAC'GTr
Cuts at: 493 1204 2922 493
Size: 711 1718 2702
Fragments arranged by size:
2702 1718 711
BsaBI GATnn'nnATC
Cuts at: 5125 5125
Size: 5131
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2938 3320 3955 275
Size: 53 83 186 936 1405 382 635 1451
Fragments arranged by size:
1451 1405 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2170 2181 3564 3962 4236 4383 4794 2170
Size: 11 1383 398 274 147 411 2507
Fragments arranged by size:
2507 1383 411 398 274 147 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4906
Fragments arranged by size:
4906 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2108 2117 2132 1186
Size: 919 3 9 15 4185
Fragments arranged by size:
4185 919 15 9 3
BseYI C'CCAG_C
Cuts at: 4334 4830 4334
Size: 496 4635
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2052 1163
Size: 573 316 4242
Fragments arranged by size:
4242 573 316
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2193 2581 3342 4370 1540
Size: 275 186 192 388 761 1028 2301
Fragments arranged by size:
2301 1028 761 388 275 192 186
BsiHKAI G_wGCw'C
Cuts at: 3192 3277 4348 3192
Size: 85 1071 3975
Fragments arranged by size:
3975 1071 85
BsmI GAATG_Cn'
Cuts at: 2400 2642 2735 2400
Size: 242 93 4796
Fragments arranged by size:
4796 242 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2303 2303
Size: 5131
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 2407 2450 3192 3277 4348 1391
Size: 399 595 22 43 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 399 85 43 22
BspEI T'CCGG_A
Cuts at: 2170 4794 2170
Size: 2624 2507
BspHI T'CATG_A
Cuts at: 3020 4750 3020
Size: 1730 3401
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2486 844
Size: 1642 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3018 1394
Size: 429 1195 3507
Fragments arranged by size:
3507 1195 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3626 3800 65
Size: 1183 2378 174 1396
Fragments arranged by size:
2378 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 3775 3962 1700
Size: 110 217 1748 187 2869
Fragments arranged by size:
2869 1748 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 2235 96
Size: 2139 2992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5131
BssSI C'ACGA_G
Cuts at: 3191 4203 3191
Size: 1012 4119
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2344 794
Size: 1550 3581
BstBI TT'CG_AA
Cuts at: 3935 3935
Size: 5131
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5131
BstYI r'GATC_y
Cuts at: 1972 2461 2828 3213 3230 4682 4768 4780 5126
Size: 489 367 385 17 1452 86 12 346
Cuts at: 5126 1972
Size: 1977
Fragments arranged by size:
1977 1452 489 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2088 2088
Size: 5131
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 513
Size: 552 939 183 3457
Fragments arranged by size:
3457 939 552 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4392
Fragments arranged by size:
4392 683 56
BtsI GCAGTG_nn'
Cuts at: 2647 3441 3461 2647
Size: 794 20 4317
Fragments arranged by size:
4317 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5096
DraI TTT'AAA
Cuts at: 2435 2435
Size: 5131
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4138 809
Size: 507 2822 1802
Fragments arranged by size:
2822 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2578 3467
Size: 54 1425 50 275 18 360 388 889
Cuts at: 3467 5096 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 388 360 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2190 2578 1537
Size: 275 378 388 4090
Fragments arranged by size:
4090 388 378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3061 1353
Size: 1708 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3718 4092 4238 387
Size: 1064 138 2129 374 146 1280
Fragments arranged by size:
2129 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 3194 3791 4578 4846 1239
Size: 393 488 1074 597 787 268 1524
Fragments arranged by size:
1524 1074 787 597 488 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2376 2376
Size: 5131
EcoO109I rG'GnC_Cy
Cuts at: 2152 2369 2152
Size: 217 4914
EcoRI G'AATT_C
Cuts at: 2440 2818 2440
Size: 378 4753
FspI TGC'GCA
Cuts at: 3637 3637
Size: 5131
HaeII r_GCGC'y
Cuts at: 1678 4278 1678
Size: 2600 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 3772 3804 3846
Size: 32 135 32 548 32 1620 32 42
Cuts at: 3846 3878 1373
Size: 32 2626
Fragments arranged by size:
2626 1620 548 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2475 2721 669
Size: 988 281 537 246 3079
Fragments arranged by size:
3079 988 537 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5131
HpaI GTT'AAC
Cuts at: 2721 2721
Size: 5131
KpnI G_GTAC'C
Cuts at: 2456 2456
Size: 5131
MfeI C'AATT_G
Cuts at: 2730 2730
Size: 5131
MluI A'CGCG_T
Cuts at: 5007 5007
Size: 5131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 4245 4429 1305
Size: 813 2127 184 2007
Fragments arranged by size:
2127 2007 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3309
Fragments arranged by size:
3309 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3089 3448 3607 518
Size: 648 1923 359 159 2042
Fragments arranged by size:
2042 1923 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2189 2353 2547 3224 4372 4617 1606
Size: 583 164 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 583 245 194 164
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3640
Fragments arranged by size:
3640 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5131
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5131
NotI GC'GGCC_GC
Cuts at: 2578 2578
Size: 5131
NspI r_CATG'y
Cuts at: 1132 1229 2489 4034 1132
Size: 97 1260 1545 2229
Fragments arranged by size:
2229 1545 1260 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5131
PciI A'CATG_T
Cuts at: 4030 4030
Size: 5131
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5131
PfoI T'CCnGG_A
Cuts at: 2419 2419
Size: 5131
PmeI GTTT'AAAC
Cuts at: 2435 2435
Size: 5131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 4713 4745 3200
Size: 32 1481 32 3586
Fragments arranged by size:
3586 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2369 2369
Size: 5131
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5131
PsiI TTA'TAA
Cuts at: 2701 2701
Size: 5131
PstI C_TGCA'G
Cuts at: 830 2349 2483 830
Size: 1519 134 3478
Fragments arranged by size:
3478 1519 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5131
RsrII CG'GwC_CG
Cuts at: 2184 2184
Size: 5131
SacII CC_GC'GG
Cuts at: 2190 2190
Size: 5131
SalI G'TCGA_C
Cuts at: 1936 2473 1936
Size: 537 4594
SbfI CC_TGCA'GG
Cuts at: 2483 2483
Size: 5131
ScaI AGT'ACT
Cuts at: 3379 5091 3379
Size: 1712 3419
SfcI C'TryA_G
Cuts at: 826 1045 2345 2479 3614 4295 4486 826
Size: 219 1300 134 1135 681 191 1471
Fragments arranged by size:
1471 1300 1135 681 219 191 134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5131
SmaI CCC'GGG
Cuts at: 1872 2458 1872
Size: 586 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3235 4136 4398 820
Size: 197 926 1292 901 262 1553
Fragments arranged by size:
1553 1292 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2922 493
Size: 2429 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5131
SphI G_CATG'C
Cuts at: 1132 2489 1132
Size: 1357 3774
SspI AAT'ATT
Cuts at: 5 52 3055 5
Size: 47 3003 2081
Fragments arranged by size:
3003 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5131
StyI C'CwwG_G
Cuts at: 513 1065 2004 2570 513
Size: 552 939 566 3074
Fragments arranged by size:
3074 939 566 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3158 3175 3328 3513 3958 325
Size: 1255 1578 17 153 185 445 1498
Fragments arranged by size:
1578 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 3377 5089
Size: 274 80 33 51 1443 258 1142 1712
Cuts at: 5089 96
Size: 138
Fragments arranged by size:
1712 1443 1142 274 258 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5091 5123 5091
Size: 32 5099
Tth111I GACn'n_nGTC
Cuts at: 1316 2134 1316
Size: 818 4313
XbaI T'CTAG_A
Cuts at: 2467 2467
Size: 5131
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5131
XmaI C'CCGG_G
Cuts at: 1870 2456 1870
Size: 586 4545
XmnI GAAnn'nnTTC
Cuts at: 3260 3260
Size: 5131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2939 276
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI RsrII SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI