Restriction map for
pFN21AE6122
(Circular) (Six-base) MAPSORT of: pFN21AE6122.seq Check: 4700 from: 1 to: 4963
pFN21AE6122 4963 bp
With 182 enzymes: SgfI *
December 8, 2014 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2773 278
Size: 53 83 186 936 1237 2468
Fragments arranged by size:
2468 1237 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2306 1937
Size: 369 4594
Acc65I G'GTAC_C
Cuts at: 2172 2284 2172
Size: 112 4851
AclI AA'CG_TT
Cuts at: 1563 3090 3463 1563
Size: 1527 373 3063
Fragments arranged by size:
3063 1527 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2259 3026 4410 4678 1632
Size: 627 767 1384 268 1917
Fragments arranged by size:
1917 1384 767 627 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4766
AflIII A'CryG_T
Cuts at: 1203 3862 4839 1203
Size: 2659 977 1327
Fragments arranged by size:
2659 1327 977
AgeI A'CCGG_T
Cuts at: 3794 3794
Size: 4963
AhdI GACnn_n'nnGTC
Cuts at: 3692 3692
Size: 4963
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4963
AlwNI CAG_nnn'CTG
Cuts at: 2706 4278 2706
Size: 1572 3391
ApaLI G'TGCA_C
Cuts at: 3020 4176 3020
Size: 1156 3807
ApoI r'AATT_y
Cuts at: 1493 2272 2498 2650 4759 1493
Size: 779 226 152 2109 1697
Fragments arranged by size:
2109 1697 779 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3517 160
Size: 872 2485 1606
Fragments arranged by size:
2485 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4963
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 2288 1870
Size: 73 309 36 4545
Fragments arranged by size:
4545 309 73 36
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2164 2197 2164
Size: 33 4930
BamHI G'GATC_C
Cuts at: 2293 2660 2293
Size: 367 4596
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 2284 3739 618
Size: 325 217 627 385 112 1455 1842
Fragments arranged by size:
1842 1455 627 385 325 217 112
BanII G_rGCy'C
Cuts at: 1391 2282 1391
Size: 891 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 928
Size: 971 120 3872
Fragments arranged by size:
3872 971 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3154 3188 1903
Size: 34 1217 34 3678
Fragments arranged by size:
3678 1217 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2855 4065 2855
Size: 1210 3753
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4963
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3574 136
Size: 107 122 71 631 605 1902 1525
Fragments arranged by size:
1902 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4958 4958
Size: 4963
BlpI GC'TnA_GC
Cuts at: 2380 2380
Size: 4963
Bme1580I G_kGCm'C
Cuts at: 1790 3024 4180 1790
Size: 1234 1156 2573
Fragments arranged by size:
2573 1234 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4963
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2230 2694 3652
Size: 416 53 83 399 24 813 464 958
Cuts at: 3652 442
Size: 1753
Fragments arranged by size:
1753 958 813 464 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3623 1239
Size: 2384 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3088 3953 4251 3088
Size: 865 298 3800
Fragments arranged by size:
3800 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 3626 882
Size: 617 516 1611 2219
Fragments arranged by size:
2219 1611 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 2754 493
Size: 711 1550 2702
Fragments arranged by size:
2702 1550 711
BsaBI GATnn'nnATC
Cuts at: 4957 4957
Size: 4963
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2770 3152 3787 275
Size: 53 83 186 936 1237 382 635 1451
Fragments arranged by size:
1451 1237 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3396 3794 4068 4215 4626 3396
Size: 398 274 147 411 3733
Fragments arranged by size:
3733 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4738
Fragments arranged by size:
4738 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4963
BseYI C'CCAG_C
Cuts at: 4166 4662 4166
Size: 496 4467
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4390
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2413 3174 4202 1540
Size: 275 186 412 761 1028 2301
Fragments arranged by size:
2301 1028 761 412 275 186
BsiHKAI G_wGCw'C
Cuts at: 3024 3109 4180 3024
Size: 85 1071 3807
Fragments arranged by size:
3807 1071 85
BsmI GAATG_Cn'
Cuts at: 2474 2567 2474
Size: 93 4870
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2282 3024 3109 4180 1391
Size: 399 492 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 85
BspEI T'CCGG_A
Cuts at: 4626 4626
Size: 4963
BspHI T'CATG_A
Cuts at: 2852 4582 2852
Size: 1730 3233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2318 844
Size: 1474 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2850 1394
Size: 429 1027 3507
Fragments arranged by size:
3507 1027 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2139 3458 3632 65
Size: 1183 891 1319 174 1396
Fragments arranged by size:
1396 1319 1183 891 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3607 3794 1700
Size: 110 1797 187 2869
Fragments arranged by size:
2869 1797 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4963
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4963
BssSI C'ACGA_G
Cuts at: 3023 4035 3023
Size: 1012 3951
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4963
BstBI TT'CG_AA
Cuts at: 3767 3767
Size: 4963
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4963
BstYI r'GATC_y
Cuts at: 1972 2293 2660 3045 3062 4514 4600 4612 4958
Size: 321 367 385 17 1452 86 12 346
Cuts at: 4958 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 321 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3472
Fragments arranged by size:
3472 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4224
Fragments arranged by size:
4224 683 56
BtsI GCAGTG_nn'
Cuts at: 2479 3273 3293 2479
Size: 794 20 4149
Fragments arranged by size:
4149 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4963
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4928
DraI TTT'AAA
Cuts at: 2267 2267
Size: 4963
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3970 809
Size: 507 2654 1802
Fragments arranged by size:
2654 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2410 3299 4928
Size: 54 1425 50 275 18 580 889 1629
Cuts at: 4928 8
Size: 43
Fragments arranged by size:
1629 1425 889 580 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2410 1537
Size: 275 598 4090
Fragments arranged by size:
4090 598 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2893 1353
Size: 1540 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3550 3924 4070 387
Size: 1064 138 435 1526 374 146 1280
Fragments arranged by size:
1526 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 3026 3623 4410 4678 1239
Size: 393 627 767 597 787 268 1524
Fragments arranged by size:
1524 787 767 627 597 393 268
EcoRI G'AATT_C
Cuts at: 2272 2650 2272
Size: 378 4585
EcoRV GAT'ATC
Cuts at: 2155 2155
Size: 4963
FspI TGC'GCA
Cuts at: 3469 3469
Size: 4963
HaeII r_GCGC'y
Cuts at: 1678 4110 1678
Size: 2432 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2139 2171 3604 3636 3678
Size: 32 135 32 567 32 1433 32 42
Cuts at: 3678 3710 1373
Size: 32 2626
Fragments arranged by size:
2626 1433 567 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2307 2553 669
Size: 988 281 369 246 3079
Fragments arranged by size:
3079 988 369 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4963
HpaI GTT'AAC
Cuts at: 2553 2553
Size: 4963
KpnI G_GTAC'C
Cuts at: 2176 2288 2176
Size: 112 4851
MfeI C'AATT_G
Cuts at: 2562 2562
Size: 4963
MluI A'CGCG_T
Cuts at: 4839 4839
Size: 4963
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4077 4261 1305
Size: 2772 184 2007
Fragments arranged by size:
2772 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3141
Fragments arranged by size:
3141 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2921 3280 3439 518
Size: 648 1755 359 159 2042
Fragments arranged by size:
2042 1755 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2121 2379 3056 4204 4449 1606
Size: 515 258 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 515 258 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3472
Fragments arranged by size:
3472 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4963
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4963
NotI GC'GGCC_GC
Cuts at: 2410 2410
Size: 4963
NspI r_CATG'y
Cuts at: 1132 1229 2321 3866 1132
Size: 97 1092 1545 2229
Fragments arranged by size:
2229 1545 1092 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4963
PciI A'CATG_T
Cuts at: 3862 3862
Size: 4963
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2208 1108
Size: 1100 3863
PmeI GTTT'AAAC
Cuts at: 2267 2267
Size: 4963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3032 3064 4545 4577 3032
Size: 32 1481 32 3418
Fragments arranged by size:
3418 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4963
PsiI TTA'TAA
Cuts at: 2533 2533
Size: 4963
PstI C_TGCA'G
Cuts at: 830 2315 830
Size: 1485 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4963
SalI G'TCGA_C
Cuts at: 1936 2305 1936
Size: 369 4594
SbfI CC_TGCA'GG
Cuts at: 2315 2315
Size: 4963
ScaI AGT'ACT
Cuts at: 3211 4923 3211
Size: 1712 3251
SfcI C'TryA_G
Cuts at: 826 1045 2311 3446 4127 4318 826
Size: 219 1266 1135 681 191 1471
Fragments arranged by size:
1471 1266 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4963
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4963
SmaI CCC'GGG
Cuts at: 1872 2254 2290 1872
Size: 382 36 4545
Fragments arranged by size:
4545 382 36
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3067 3968 4230 820
Size: 197 926 1124 901 262 1553
Fragments arranged by size:
1553 1124 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2754 493
Size: 2261 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4963
SphI G_CATG'C
Cuts at: 1132 2321 1132
Size: 1189 3774
SspI AAT'ATT
Cuts at: 5 52 2887 5
Size: 47 2835 2081
Fragments arranged by size:
2835 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4963
StyI C'CwwG_G
Cuts at: 513 1065 2004 2402 513
Size: 552 939 398 3074
Fragments arranged by size:
3074 939 552 398
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2990 3007 3160 3345 3790 325
Size: 1255 1410 17 153 185 445 1498
Fragments arranged by size:
1498 1410 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3209 4921 96
Size: 274 80 33 51 1443 1232 1712 138
Fragments arranged by size:
1712 1443 1232 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4923 4955 4923
Size: 32 4931
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4963
XbaI T'CTAG_A
Cuts at: 2299 2299
Size: 4963
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4963
XmaI C'CCGG_G
Cuts at: 1870 2252 2288 1870
Size: 382 36 4545
Fragments arranged by size:
4545 382 36
XmnI GAAnn'nnTTC
Cuts at: 3092 3092
Size: 4963
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2771 276
Size: 53 83 186 936 1237 2468
Fragments arranged by size:
2468 1237 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI