Restriction map for
pFN21AE6111
(Circular) (Six-base) MAPSORT of: pFN21AE6111.seq Check: 1495 from: 1 to: 5131
pFN21AE6111 5131 bp
With 182 enzymes: SgfI *
December 8, 2014 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2941 278
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 1937
Size: 537 4594
Acc65I G'GTAC_C
Cuts at: 2452 2452
Size: 5131
AclI AA'CG_TT
Cuts at: 1563 3258 3631 1563
Size: 1695 373 3063
Fragments arranged by size:
3063 1695 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3194 4578 4846 1632
Size: 1562 1384 268 1917
Fragments arranged by size:
1917 1562 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4934
AflIII A'CryG_T
Cuts at: 1203 4030 5007 1203
Size: 2827 977 1327
Fragments arranged by size:
2827 1327 977
AgeI A'CCGG_T
Cuts at: 3962 3962
Size: 5131
AhdI GACnn_n'nnGTC
Cuts at: 2284 3860 2284
Size: 1576 3555
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5131
AlwNI CAG_nnn'CTG
Cuts at: 2029 2874 4446 2029
Size: 845 1572 2714
Fragments arranged by size:
2714 1572 845
ApaI G_GGCC'C
Cuts at: 2317 2317
Size: 5131
ApaLI G'TGCA_C
Cuts at: 3188 4344 3188
Size: 1156 3975
ApoI r'AATT_y
Cuts at: 1493 2440 2666 2818 4927 1493
Size: 947 226 152 2109 1697
Fragments arranged by size:
2109 1697 947 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3685 160
Size: 872 2653 1606
Fragments arranged by size:
2653 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2317 2456 1870
Size: 73 114 260 139 4545
Fragments arranged by size:
4545 260 139 114 73
BamHI G'GATC_C
Cuts at: 2461 2828 2461
Size: 367 4764
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 2452 3907 618
Size: 325 217 627 537 128 1455 1842
Fragments arranged by size:
1842 1455 627 537 325 217 128
BanII G_rGCy'C
Cuts at: 1391 2317 2360 2450 1391
Size: 926 43 90 4072
Fragments arranged by size:
4072 926 90 43
BbeI G_GCGC'C
Cuts at: 2328 2328
Size: 5131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2107 2141 3322 3356 1903
Size: 34 170 34 1181 34 3678
Fragments arranged by size:
3678 1181 170 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3023 4233 3023
Size: 1210 3921
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5131
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2222 3742 136
Size: 107 122 71 631 605 550 1520 1525
Fragments arranged by size:
1525 1520 631 605 550 122 107 71
BglII A'GATC_T
Cuts at: 5126 5126
Size: 5131
BlpI GC'TnA_GC
Cuts at: 2421 2548 2421
Size: 127 5004
Bme1580I G_kGCm'C
Cuts at: 1790 2317 3192 4348 1790
Size: 527 875 1156 2573
Fragments arranged by size:
2573 1156 875 527
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5131
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2862 3820 442
Size: 416 53 83 399 24 1445 958 1753
Fragments arranged by size:
1753 1445 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3791 1239
Size: 2552 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3256 4121 4419 3256
Size: 865 298 3968
Fragments arranged by size:
3968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3794 882
Size: 617 2295 2219
Fragments arranged by size:
2295 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2922 493
Size: 711 1718 2702
Fragments arranged by size:
2702 1718 711
BsaBI GATnn'nnATC
Cuts at: 5125 5125
Size: 5131
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2178 2325 2340 2938
Size: 53 83 186 936 645 147 15 598
Cuts at: 2938 3320 3955 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 645 635 598 382 186 147
83 53 15
BsaWI w'CCGG_w
Cuts at: 3564 3962 4236 4383 4794 3564
Size: 398 274 147 411 3901
Fragments arranged by size:
3901 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 1178
Size: 30 165 30 834 30 4042
Fragments arranged by size:
4042 834 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5131
BseYI C'CCAG_C
Cuts at: 2108 4334 4830 2108
Size: 2226 496 2409
Fragments arranged by size:
2409 2226 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 1163
Size: 573 312 4246
Fragments arranged by size:
4246 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2581 3342 4370 1540
Size: 275 186 580 761 1028 2301
Fragments arranged by size:
2301 1028 761 580 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 3192 3277 4348 2307
Size: 885 85 1071 3090
Fragments arranged by size:
3090 1071 885 85
BsmI GAATG_Cn'
Cuts at: 2642 2735 2642
Size: 93 5038
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2317 2360 2450 3192 3277 4348
Size: 399 517 10 43 90 742 85 1071
Cuts at: 4348 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 517 399 90 85 43
10
BspEI T'CCGG_A
Cuts at: 4794 4794
Size: 5131
BspHI T'CATG_A
Cuts at: 2202 3020 4750 2202
Size: 818 1730 2583
Fragments arranged by size:
2583 1730 818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2486 844
Size: 1642 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2253 2425 3018 1394
Size: 429 430 172 593 3507
Fragments arranged by size:
3507 593 430 429 172
BsrDI GCAATG_nn'
Cuts at: 65 1248 3626 3800 65
Size: 1183 2378 174 1396
Fragments arranged by size:
2378 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3775 3962 1700
Size: 110 1965 187 2869
Fragments arranged by size:
2869 1965 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5131
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5131
BssSI C'ACGA_G
Cuts at: 3191 4203 3191
Size: 1012 4119
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5131
BstBI TT'CG_AA
Cuts at: 3935 3935
Size: 5131
BstEII G'GTnAC_C
Cuts at: 1184 2155 1184
Size: 971 4160
BstXI CCAn_nnnn'nTGG
Cuts at: 2089 2089
Size: 5131
BstYI r'GATC_y
Cuts at: 1972 2461 2828 3213 3230 4682 4768 4780 5126
Size: 489 367 385 17 1452 86 12 346
Cuts at: 5126 1972
Size: 1977
Fragments arranged by size:
1977 1452 489 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2352 513
Size: 552 1287 3292
Fragments arranged by size:
3292 1287 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4392
Fragments arranged by size:
4392 683 56
BtsI GCAGTG_nn'
Cuts at: 2647 3441 3461 2647
Size: 794 20 4317
Fragments arranged by size:
4317 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5096
DraI TTT'AAA
Cuts at: 2435 2435
Size: 5131
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4138 809
Size: 507 2822 1802
Fragments arranged by size:
2822 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2578 3467
Size: 54 1425 50 275 18 393 355 889
Cuts at: 3467 5096 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 393 355 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2578 1537
Size: 275 766 4090
Fragments arranged by size:
4090 766 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3061 1353
Size: 1708 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3718 4092 4238 387
Size: 1064 138 2129 374 146 1280
Fragments arranged by size:
2129 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3194 3791 4578 4846 1239
Size: 393 1562 597 787 268 1524
Fragments arranged by size:
1562 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 2411
Size: 5131
EcoO109I rG'GnC_Cy
Cuts at: 2314 2314
Size: 5131
EcoRI G'AATT_C
Cuts at: 2440 2818 2440
Size: 378 4753
FspI TGC'GCA
Cuts at: 3637 3637
Size: 5131
HaeII r_GCGC'y
Cuts at: 1678 2097 2166 2328 2421 4278 1678
Size: 419 69 162 93 1857 2531
Fragments arranged by size:
2531 1857 419 162 93 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2220 2252 2270 2302 3772
Size: 32 135 32 648 32 18 32 1470
Cuts at: 3772 3804 3846 3878 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1470 648 135 42 32 32 32
32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 2475 2721 669
Size: 988 281 537 246 3079
Fragments arranged by size:
3079 988 537 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5131
HpaI GTT'AAC
Cuts at: 2721 2721
Size: 5131
KasI G'GCGC_C
Cuts at: 2324 2324
Size: 5131
KpnI G_GTAC'C
Cuts at: 2456 2456
Size: 5131
MfeI C'AATT_G
Cuts at: 2730 2730
Size: 5131
MluI A'CGCG_T
Cuts at: 5007 5007
Size: 5131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4245 4429 1305
Size: 2940 184 2007
Fragments arranged by size:
2940 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3309
Fragments arranged by size:
3309 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 3089 3448 3607 518
Size: 648 951 972 359 159 2042
Fragments arranged by size:
2042 972 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2085 2354 2547 3224 4372 4617 1606
Size: 420 59 269 193 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 420 269 245 193 59
NarI GG'CG_CC
Cuts at: 2325 2325
Size: 5131
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4579
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5131
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5131
NotI GC'GGCC_GC
Cuts at: 2578 2578
Size: 5131
NspI r_CATG'y
Cuts at: 1132 1229 2489 4034 1132
Size: 97 1260 1545 2229
Fragments arranged by size:
2229 1545 1260 97
PasI CC'CwG_GG
Cuts at: 1348 2413 1348
Size: 1065 4066
PciI A'CATG_T
Cuts at: 4030 4030
Size: 5131
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5131
PmeI GTTT'AAAC
Cuts at: 2435 2435
Size: 5131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 4713 4745 3200
Size: 32 1481 32 3586
Fragments arranged by size:
3586 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5131
PsiI TTA'TAA
Cuts at: 2701 2701
Size: 5131
PspOMI G'GGCC_C
Cuts at: 2313 2313
Size: 5131
PspXI vC'TCGA_Gb
Cuts at: 2057 2057
Size: 5131
PstI C_TGCA'G
Cuts at: 830 2015 2483 830
Size: 1185 468 3478
Fragments arranged by size:
3478 1185 468
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5131
SacII CC_GC'GG
Cuts at: 2355 2355
Size: 5131
SalI G'TCGA_C
Cuts at: 1936 2473 1936
Size: 537 4594
SbfI CC_TGCA'GG
Cuts at: 2483 2483
Size: 5131
ScaI AGT'ACT
Cuts at: 3379 5091 3379
Size: 1712 3419
SfcI C'TryA_G
Cuts at: 826 1045 2011 2479 3614 4295 4486 826
Size: 219 966 468 1135 681 191 1471
Fragments arranged by size:
1471 1135 966 681 468 219 191
SfoI GGC'GCC
Cuts at: 2326 2326
Size: 5131
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5131
SmaI CCC'GGG
Cuts at: 1872 2319 2458 1872
Size: 447 139 4545
Fragments arranged by size:
4545 447 139
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 3235 4136 4398 820
Size: 197 926 114 1178 901 262 1553
Fragments arranged by size:
1553 1178 926 901 262 197 114
SnaBI TAC'GTA
Cuts at: 493 2922 493
Size: 2429 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5131
SphI G_CATG'C
Cuts at: 1132 2489 1132
Size: 1357 3774
SspI AAT'ATT
Cuts at: 5 52 3055 5
Size: 47 3003 2081
Fragments arranged by size:
3003 2081 47
StuI AGG'CCT
Cuts at: 1517 2232 1517
Size: 715 4416
StyI C'CwwG_G
Cuts at: 513 1065 2166 2274 2570 513
Size: 552 1101 108 296 3074
Fragments arranged by size:
3074 1101 552 296 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3158 3175 3328 3513 3958 325
Size: 1255 1578 17 153 185 445 1498
Fragments arranged by size:
1578 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3377 5089 96
Size: 274 80 33 51 1443 1400 1712 138
Fragments arranged by size:
1712 1443 1400 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5091 5123 5091
Size: 32 5099
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5131
XbaI T'CTAG_A
Cuts at: 2467 2467
Size: 5131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 2116
Size: 5131
XhoI C'TCGA_G
Cuts at: 1943 2057 1943
Size: 114 5017
XmaI C'CCGG_G
Cuts at: 1870 2317 2456 1870
Size: 447 139 4545
Fragments arranged by size:
4545 447 139
XmnI GAAnn'nnTTC
Cuts at: 3260 3260
Size: 5131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2939 276
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI