Restriction map for
pFN21AE6093
(Circular) (Six-base) MAPSORT of: pFN21AE6093.seq Check: 978 from: 1 to: 5683
pFN21AE6093 5683 bp
With 182 enzymes: SgfI *
December 8, 2014 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5130 5398 1632
Size: 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2229 4582 5559 1203
Size: 1026 2353 977 1327
Fragments arranged by size:
2353 1327 1026 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 2756 4412 2756
Size: 1656 4027
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2487 1184
Size: 1303 4380
AlwNI CAG_nnn'CTG
Cuts at: 3426 4998 3426
Size: 1572 4111
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2056 2081 2242 2992 3218 3370 5479 1493
Size: 563 25 161 750 226 152 2109 1697
Fragments arranged by size:
2109 1697 750 563 226 161 152 25
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 1870
Size: 73 1065 4545
Fragments arranged by size:
4545 1065 73
BamHI G'GATC_C
Cuts at: 2696 3013 3380 2696
Size: 317 367 4999
Fragments arranged by size:
4999 367 317
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3004 4459 618
Size: 325 217 627 1217 1455 1842
Fragments arranged by size:
1842 1455 1217 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2834 3002 1391
Size: 1443 168 4072
Fragments arranged by size:
4072 1443 168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2752 928
Size: 971 853 3859
Fragments arranged by size:
3859 971 853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 2410 1548
Size: 862 4821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2121 5678 2121
Size: 3557 2126
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 3744 4900 1790
Size: 1954 1156 2573
Fragments arranged by size:
2573 1954 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2469 2754 3414
Size: 416 53 83 399 24 1052 285 660
Cuts at: 3414 4372 442
Size: 958 1753
Fragments arranged by size:
1753 1052 958 660 416 399 285 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 2909 4343 1239
Size: 1091 579 1434 2579
Fragments arranged by size:
2579 1434 1091 579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2781 4346 882
Size: 617 1282 1565 2219
Fragments arranged by size:
2219 1565 1282 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5458
Fragments arranged by size:
5458 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5683
BseYI C'CCAG_C
Cuts at: 2493 4886 5382 2493
Size: 2393 496 2794
Fragments arranged by size:
2794 2393 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3744 3829 4900 3744
Size: 85 1071 4527
Fragments arranged by size:
4527 1071 85
BsmI GAATG_Cn'
Cuts at: 2387 3194 3287 2387
Size: 807 93 4783
Fragments arranged by size:
4783 807 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2834 3002 3744 3829 4900 1391
Size: 399 1044 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 1044 742 399 168 85
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 2454 3572 5302 2454
Size: 1118 1730 2835
Fragments arranged by size:
2835 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2014 3038 844
Size: 1170 1024 3489
Fragments arranged by size:
3489 1170 1024
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2181 4178 4352 65
Size: 1183 933 1997 174 1396
Fragments arranged by size:
1997 1396 1183 933 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 4327 4514 1700
Size: 110 395 2122 187 2869
Fragments arranged by size:
2869 2122 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 2226 96
Size: 2130 3553
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 2890 1184
Size: 1706 3977
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 2293
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 2121 2696 3013 3380 3765 3782 5234 5320
Size: 149 575 317 367 385 17 1452 86
Cuts at: 5320 5332 5678 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 575 385 367 346 317 149
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2951 2951
Size: 5683
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3993 4013 3199
Size: 794 20 4869
Fragments arranged by size:
4869 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648
Size: 54 1425 50 275 18 1300 889 1629
Cuts at: 5648 8
Size: 43
Fragments arranged by size:
1629 1425 1300 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 2278 2906 3613 1353
Size: 782 143 628 707 3423
Fragments arranged by size:
3423 782 707 628 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2909 3746 4343 5130 5398 1239
Size: 393 698 579 837 597 787 268 1524
Fragments arranged by size:
1524 837 787 698 597 579 393 268
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 4830 1678
Size: 3152 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2328 2360 2449 2481 2742
Size: 32 135 32 756 32 89 32 261
Cuts at: 2742 2774 4324 4356 4398 4430 1373
Size: 32 1550 32 42 32 2626
Fragments arranged by size:
2626 1550 756 261 135 89 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2299 3027 3273 669
Size: 988 281 361 728 246 3079
Fragments arranged by size:
3079 988 728 361 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2659 2997 4797 4981 1305
Size: 1354 338 1800 184 2007
Fragments arranged by size:
2007 1800 1354 338 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2228 2380 2469 2825 3641 4000
Size: 648 1056 6 152 89 356 816 359
Cuts at: 4000 4159 518
Size: 159 2042
Fragments arranged by size:
2042 1056 816 648 359 356 159 152
89 6
MspA1I CmG'CkG
Cuts at: 1606 3099 3776 4924 5169 1606
Size: 1493 677 1148 245 2120
Fragments arranged by size:
2120 1493 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5131
NdeI CA'TA_TG
Cuts at: 387 2152 387
Size: 1765 3918
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2233 3041 4586 1132
Size: 97 1004 808 1545 2229
Fragments arranged by size:
2229 1545 1004 808 97
PasI CC'CwG_GG
Cuts at: 1348 2591 1348
Size: 1243 4440
PciI A'CATG_T
Cuts at: 2229 4582 2229
Size: 2353 3330
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 3035 830
Size: 2205 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2277 2803 2850 2917 3031 4166 4847
Size: 219 1232 526 47 67 114 1135 681
Cuts at: 4847 5038 826
Size: 191 1471
Fragments arranged by size:
1471 1232 1135 681 526 219 191 114
67 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 2092 3122 513
Size: 552 1027 1030 3074
Fragments arranged by size:
3074 1030 1027 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3710 3727 3880 4065 4510 325
Size: 1255 2130 17 153 185 445 1498
Fragments arranged by size:
2130 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2155 2226 3929
Size: 274 80 33 51 1443 178 71 1703
Cuts at: 3929 5641 96
Size: 1712 138
Fragments arranged by size:
1712 1703 1443 274 178 138 80 71
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI