Restriction map for
pFN21AE6090
(Circular) (Six-base) MAPSORT of: pFN21AE6090.seq Check: 3608 from: 1 to: 5680
pFN21AE6090 5680 bp
With 182 enzymes: SgfI *
December 8, 2014 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3743 5127 5395 1632
Size: 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 2726 2737 4579 5556 1203
Size: 1523 11 1842 977 1327
Fragments arranged by size:
1842 1523 1327 977 11
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 2219 2740 1166
Size: 1053 521 4106
Fragments arranged by size:
4106 1053 521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 3423 4995 3423
Size: 1572 4108
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 3005 1870
Size: 73 1062 4545
Fragments arranged by size:
4545 1062 73
BamHI G'GATC_C
Cuts at: 2058 3010 3377 2058
Size: 952 367 4361
Fragments arranged by size:
4361 952 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2854 3001 4456 618
Size: 325 217 627 1067 147 1455 1842
Fragments arranged by size:
1842 1455 1067 627 325 217 147
BanII G_rGCy'C
Cuts at: 1391 2999 1391
Size: 1608 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2681 928
Size: 971 782 3927
Fragments arranged by size:
3927 971 782
BbvCI CC'TCA_GC
Cuts at: 2720 2720
Size: 5680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 2154 1548
Size: 606 5074
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2207 4291 136
Size: 107 122 71 631 605 535 2084 1525
Fragments arranged by size:
2084 1525 631 605 535 122 107 71
BglII A'GATC_T
Cuts at: 2901 5675 2901
Size: 2774 2906
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2814 3741 4897 1790
Size: 1024 927 1156 2573
Fragments arranged by size:
2573 1156 1024 927
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2020 3411 4369
Size: 416 53 83 399 24 603 1391 958
Cuts at: 4369 442
Size: 1753
Fragments arranged by size:
1753 1391 958 603 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
Bpu10I CC'TnA_GC
Cuts at: 2720 2720
Size: 5680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2696 3805 4670 4968 2696
Size: 1109 865 298 3408
Fragments arranged by size:
3408 1109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2231 4343 882
Size: 617 732 2112 2219
Fragments arranged by size:
2219 2112 732 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 1178
Size: 30 165 30 621 30 4804
Fragments arranged by size:
4804 621 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 1186
Size: 843 4837
BseYI C'CCAG_C
Cuts at: 2053 4883 5379 2053
Size: 2830 496 2354
Fragments arranged by size:
2830 2354 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2553 3741 3826 4897 2553
Size: 1188 85 1071 3336
Fragments arranged by size:
3336 1188 1071 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2553 2814 2999 3741 3826 4897 1391
Size: 399 763 261 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 763 742 399 261 185 85
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 2698 3569 5299 2698
Size: 871 1730 3079
Fragments arranged by size:
3079 1730 871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3035 844
Size: 2191 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2367 2646 4175 4349 65
Size: 1183 1119 279 1529 174 1396
Fragments arranged by size:
1529 1396 1183 1119 279 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 2112 96
Size: 2016 3664
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2221 2221
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2058 2901 3010 3377 3762 3779 5231 5317
Size: 86 843 109 367 385 17 1452 86
Cuts at: 5317 5329 5675 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 843 385 367 346 109 86
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2777 487
Size: 683 56 1551 3390
Fragments arranged by size:
3390 1551 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3990 4010 3196
Size: 794 20 4866
Fragments arranged by size:
4866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2642 4687 809
Size: 507 1326 2045 1802
Fragments arranged by size:
2045 1802 1326 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2208 2382
Size: 54 1425 50 275 18 369 9 174
Cuts at: 2382 2800 3127 4016 5645 8
Size: 418 327 889 1629 43
Fragments arranged by size:
1629 1425 889 418 369 327 275 174
54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2949 3610 1353
Size: 1596 661 3423
Fragments arranged by size:
3423 1596 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3743 4340 5127 5395 1239
Size: 393 2111 597 787 268 1524
Fragments arranged by size:
2111 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2412 4827 1678
Size: 734 2415 2531
Fragments arranged by size:
2531 2415 734
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 4321 4353 4395
Size: 32 135 32 699 32 2018 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 2018 699 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2210 2384 10
Size: 54 1425 343 369 9 174 3306
Fragments arranged by size:
3306 1425 369 343 174 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2309 2336 2740 2742 3638 3997
Size: 648 1053 90 27 404 2 896 359
Cuts at: 3997 4156 518
Size: 159 2042
Fragments arranged by size:
2042 1053 896 648 404 359 159 90
27 2
MspA1I CmG'CkG
Cuts at: 1606 3096 3773 4921 5166 1606
Size: 1490 677 1148 245 2120
Fragments arranged by size:
2120 1490 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5128
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2730 2741 3038 4583 1132
Size: 97 1501 11 297 1545 2229
Fragments arranged by size:
2229 1545 1501 297 97 11
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 2726 2737 4579 2726
Size: 11 1842 3827
Fragments arranged by size:
3827 1842 11
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2713 1613
Size: 1100 4580
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 3032 830
Size: 2202 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 3028 4163 4844 5035 826
Size: 219 1983 1135 681 191 1471
Fragments arranged by size:
1983 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2207 2207
Size: 5680
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2711 3784 4685 4947 820
Size: 197 926 768 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 768 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 2596 2925 3604 5
Size: 47 2544 329 679 2081
Fragments arranged by size:
2544 2081 679 329 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 3119 513
Size: 552 2054 3074
Fragments arranged by size:
3074 2054 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2039 2321 3707 3724 3877 4062 4507
Size: 1255 459 282 1386 17 153 185 445
Cuts at: 4507 325
Size: 1498
Fragments arranged by size:
1498 1386 1255 459 445 282 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 3926 5638
Size: 274 80 33 51 1443 135 1814 1712
Cuts at: 5638 96
Size: 138
Fragments arranged by size:
1814 1712 1443 274 138 135 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 5640 5672 2024
Size: 32 3584 32 2032
Fragments arranged by size:
3584 2032 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2218 2218
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 2076 3809 2076
Size: 1733 3947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfoI SrfI SwaI