Restriction map for
pFN21AE6089
(Circular) (Six-base) MAPSORT of: pFN21AE6089.seq Check: 4133 from: 1 to: 5680
pFN21AE6089 5680 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2012 3743 5127 5395 1632
Size: 380 1731 1384 268 1917
Fragments arranged by size:
1917 1731 1384 380 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 2882 1166
Size: 1716 3964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2060 1184
Size: 876 4804
AlwNI CAG_nnn'CTG
Cuts at: 3423 4995 3423
Size: 1572 4108
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2075 2967 2989 3215 3367 5476 1493
Size: 582 892 22 226 152 2109 1697
Fragments arranged by size:
2109 1697 892 582 226 152 22
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 3005 1870
Size: 73 1062 4545
Fragments arranged by size:
4545 1062 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2939 3001 4456 618
Size: 325 217 627 1152 62 1455 1842
Fragments arranged by size:
1842 1455 1152 627 325 217 62
BanII G_rGCy'C
Cuts at: 1391 2175 2999 1391
Size: 784 824 4072
Fragments arranged by size:
4072 824 784
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 928
Size: 971 116 4593
Fragments arranged by size:
4593 971 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2191 3572 4782 2191
Size: 1381 1210 3089
Fragments arranged by size:
3089 1381 1210
BclI T'GATC_A
Cuts at: 1548 2622 1548
Size: 1074 4606
BfrBI ATG'CAT
Cuts at: 2047 2047
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2944 3741 4897 1790
Size: 1154 797 1156 2573
Fragments arranged by size:
2573 1156 1154 797
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3411 4369 442
Size: 416 53 83 399 24 1994 958 1753
Fragments arranged by size:
1994 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2938 3805 4670 4968 2938
Size: 867 865 298 3650
Fragments arranged by size:
3650 867 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2202 3471 493
Size: 711 998 1269 2702
Fragments arranged by size:
2702 1269 998 711
BsaBI GATnn'nnATC
Cuts at: 2109 5674 2109
Size: 3565 2115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2821 1186
Size: 925 710 4045
Fragments arranged by size:
4045 925 710
BseYI C'CCAG_C
Cuts at: 4883 5379 4883
Size: 496 5184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3741 3826 4897 3741
Size: 85 1071 4524
Fragments arranged by size:
4524 1071 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2944 2999 3741 3826 4897 1391
Size: 399 385 769 55 742 85 1071 2174
Fragments arranged by size:
2174 1071 769 742 399 385 85 55
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3035 844
Size: 2191 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2287
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 3010 3377 3762 3779 5231 5317 5329 5675
Size: 1038 367 385 17 1452 86 12 346
Cuts at: 5675 1972
Size: 1977
Fragments arranged by size:
1977 1452 1038 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2844 2844
Size: 5680
BtgI C'CryG_G
Cuts at: 513 1065 2394 513
Size: 552 1329 3799
Fragments arranged by size:
3799 1329 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3990 4010 3196
Size: 794 20 4866
Fragments arranged by size:
4866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DraIII CAC_nnn'GTG
Cuts at: 2413 2413
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3127 4016 5645
Size: 54 1425 50 275 18 1297 889 1629
Cuts at: 5645 8
Size: 43
Fragments arranged by size:
1629 1425 1297 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 2549 3610 1353
Size: 878 318 1061 3423
Fragments arranged by size:
3423 1061 878 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 3743 4340 5127 5395 1239
Size: 393 380 1731 597 787 268 1524
Fragments arranged by size:
1731 1524 787 597 393 380 268
EcoRI G'AATT_C
Cuts at: 2075 2989 3367 2075
Size: 914 378 4388
Fragments arranged by size:
4388 914 378
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 4827 1678
Size: 3149 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 4321 4353 4395
Size: 32 135 32 671 32 2046 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 2046 671 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 2537 3279 2537
Size: 742 4938
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2292 4794 4978 1305
Size: 987 2502 184 2007
Fragments arranged by size:
2502 2007 987 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3858
Fragments arranged by size:
3858 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2342 2882 3638 3997 4156 518
Size: 648 1014 162 540 756 359 159 2042
Fragments arranged by size:
2042 1014 756 648 540 359 162 159
MspA1I CmG'CkG
Cuts at: 1606 3096 3773 4921 5166 1606
Size: 1490 677 1148 245 2120
Fragments arranged by size:
2120 1490 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2394 513
Size: 552 1329 3799
Fragments arranged by size:
3799 1329 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NsiI A_TGCA'T
Cuts at: 2049 2049
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 3038 4583 1132
Size: 97 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2413 1108
Size: 1305 4375
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 3032 830
Size: 2202 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SapI GCTCTTCn'nnn_
Cuts at: 2549 2549
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SexAI A'CCwGG_T
Cuts at: 2148 2148
Size: 5680
SfcI C'TryA_G
Cuts at: 826 1045 2911 3028 4163 4844 5035 826
Size: 219 1866 117 1135 681 191 1471
Fragments arranged by size:
1866 1471 1135 681 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2917 3784 4685 4947 820
Size: 197 926 974 867 901 262 1553
Fragments arranged by size:
1553 974 926 901 867 262 197
SnaBI TAC'GTA
Cuts at: 493 2202 3471 493
Size: 1709 1269 2702
Fragments arranged by size:
2702 1709 1269
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 2296 2503 3604 5
Size: 47 2244 207 1101 2081
Fragments arranged by size:
2244 2081 1101 207 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2394 3119 513
Size: 552 1329 725 3074
Fragments arranged by size:
3074 1329 725 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2905 3707 3724 3877 4062 4507 325
Size: 1255 1325 802 17 153 185 445 1498
Fragments arranged by size:
1498 1325 1255 802 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 2817 3926
Size: 274 80 33 51 1443 369 471 1109
Cuts at: 3926 5638 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1109 471 369 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 2079 2753 2968 3809 2079
Size: 674 215 841 3950
Fragments arranged by size:
3950 841 674 215
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI XcmI