Restriction map for
pFN21AE6079
(Circular) (Six-base) MAPSORT of: pFN21AE6079.seq Check: 7036 from: 1 to: 5677
pFN21AE6079 5677 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2178 2178
Size: 5677
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2704 3740 5124 5392 1632
Size: 1072 1036 1384 268 1917
Fragments arranged by size:
1917 1384 1072 1036 268
AflII C'TTAA_G
Cuts at: 820 1017 2134 820
Size: 197 1117 4363
Fragments arranged by size:
4363 1117 197
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 2370 4508 2370
Size: 2138 3539
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 2030 1166
Size: 864 4813
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2411 1184
Size: 1227 4450
AlwNI CAG_nnn'CTG
Cuts at: 3420 4992 3420
Size: 1572 4105
ApaI G_GGCC'C
Cuts at: 2520 2520
Size: 5677
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2039 2986 3212 3364 5473 1493
Size: 546 947 226 152 2109 1697
Fragments arranged by size:
2109 1697 947 546 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 3002 1870
Size: 73 990 69 4545
Fragments arranged by size:
4545 990 73 69
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2447 2998 4453 618
Size: 325 217 627 660 551 1455 1842
Fragments arranged by size:
1842 1455 660 627 551 325 217
BanII G_rGCy'C
Cuts at: 1391 2109 2520 2996 1391
Size: 718 411 476 4072
Fragments arranged by size:
4072 718 476 411
BbeI G_GCGC'C
Cuts at: 2451 2451
Size: 5677
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2308 928
Size: 971 409 4297
Fragments arranged by size:
4297 971 409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 2526 1548
Size: 978 4699
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 2695 3094 2695
Size: 399 5278
Bme1580I G_kGCm'C
Cuts at: 1790 2520 3738 4894 1790
Size: 730 1218 1156 2573
Fragments arranged by size:
2573 1218 1156 730
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2787 3408 4366
Size: 416 53 83 399 24 1370 621 958
Cuts at: 4366 442
Size: 1753
Fragments arranged by size:
1753 1370 958 621 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2538 4337 1239
Size: 1299 1799 2579
Fragments arranged by size:
2579 1799 1299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2448 3484 3866 4501
Size: 53 83 186 936 915 1036 382 635
Cuts at: 4501 275
Size: 1451
Fragments arranged by size:
1451 1036 936 915 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2370 4110 4508 4782 4929 5340 2370
Size: 1740 398 274 147 411 2707
Fragments arranged by size:
2707 1740 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5452
Fragments arranged by size:
5452 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5677
BseYI C'CCAG_C
Cuts at: 4880 5376 4880
Size: 496 5181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 1163
Size: 573 420 4684
Fragments arranged by size:
4684 573 420
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 2345 3188 3281 2345
Size: 843 93 4741
Fragments arranged by size:
4741 843 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2520 2996 3738 3823 4894 1391
Size: 399 319 411 476 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 411 399 319 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 3032 844
Size: 1334 854 3489
Fragments arranged by size:
3489 1334 854
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2142 4172 4346 65
Size: 1183 894 2030 174 1396
Fragments arranged by size:
2030 1396 1183 894 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2370 4321 4508 1700
Size: 110 560 1951 187 2869
Fragments arranged by size:
2869 1951 560 187 110
BsrGI T'GTAC_A
Cuts at: 96 2781 96
Size: 2685 2992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 2599 1184
Size: 1415 4262
BstXI CCAn_nnnn'nTGG
Cuts at: 2596 2596
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2856 2919 513
Size: 552 939 852 63 3271
Fragments arranged by size:
3271 939 852 552 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 3987 4007 3193
Size: 794 20 4863
Fragments arranged by size:
4863 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2433 2981 2433
Size: 548 5129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642
Size: 54 1425 50 275 18 1294 889 1629
Cuts at: 5642 8
Size: 43
Fragments arranged by size:
1629 1425 1294 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2762 3607 1353
Size: 1409 845 3423
Fragments arranged by size:
3423 1409 845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2538 2704 3740 4337 5124 5392 1239
Size: 393 906 166 1036 597 787 268 1524
Fragments arranged by size:
1524 1036 906 787 597 393 268 166
EcoO109I rG'GnC_Cy
Cuts at: 2517 2929 2517
Size: 412 5265
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2451 4824 1678
Size: 773 2373 2531
Fragments arranged by size:
2531 2373 773
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 4318 4350 4392
Size: 32 135 32 577 32 2137 32 42
Cuts at: 4392 4424 1373
Size: 32 2626
Fragments arranged by size:
2626 2137 577 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KasI G'GCGC_C
Cuts at: 2447 2447
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 2028 3276 2028
Size: 1248 4429
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2605 4791 4975 1305
Size: 1300 2186 184 2007
Fragments arranged by size:
2186 2007 1300 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3855
Fragments arranged by size:
3855 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2156 2180 2783 2963 3635 3994
Size: 648 864 126 24 603 180 672 359
Cuts at: 3994 4153 518
Size: 159 2042
Fragments arranged by size:
2042 864 672 648 603 359 180 159
126 24
MspA1I CmG'CkG
Cuts at: 1606 3093 3770 4918 5163 1606
Size: 1487 677 1148 245 2120
Fragments arranged by size:
2120 1487 1148 677 245
NarI GG'CG_CC
Cuts at: 2448 2448
Size: 5677
NcoI C'CATG_G
Cuts at: 513 1065 2004 2919 513
Size: 552 939 915 3271
Fragments arranged by size:
3271 939 915 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2179 2788 3035 4580 1132
Size: 97 950 609 247 1545 2229
Fragments arranged by size:
2229 1545 950 609 247 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5677
PfoI T'CCnGG_A
Cuts at: 2211 2211
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2929 2929
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PspOMI G'GGCC_C
Cuts at: 2516 2516
Size: 5677
PstI C_TGCA'G
Cuts at: 830 3029 830
Size: 2199 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SanDI GG'GwC_CC
Cuts at: 2929 2929
Size: 5677
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 2309 3925 5637 2309
Size: 1616 1712 2349
Fragments arranged by size:
2349 1712 1616
SfcI C'TryA_G
Cuts at: 826 1045 2010 2700 2875 3025 4160 4841 5032
Size: 219 965 690 175 150 1135 681 191
Cuts at: 5032 826
Size: 1471
Fragments arranged by size:
1471 1135 965 690 681 219 191 175
150
SfoI GGC'GCC
Cuts at: 2449 2449
Size: 5677
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 2935 3004 1872
Size: 1063 69 4545
Fragments arranged by size:
4545 1063 69
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2134 3781 4682 4944 820
Size: 197 926 191 1647 901 262 1553
Fragments arranged by size:
1647 1553 926 901 262 197 191
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 2717 1517
Size: 1200 4477
StyI C'CwwG_G
Cuts at: 513 1065 2004 2844 2919 2970 3116 513
Size: 552 939 840 75 51 146 3074
Fragments arranged by size:
3074 939 840 552 146 75 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2447 3704 3721 3874 4059 4504 325
Size: 1255 867 1257 17 153 185 445 1498
Fragments arranged by size:
1498 1257 1255 867 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2307 2781
Size: 274 80 33 51 1443 207 123 474
Cuts at: 2781 3923 5635 96
Size: 1142 1712 138
Fragments arranged by size:
1712 1443 1142 474 274 207 138 123
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 2933 3002 1870
Size: 1063 69 4545
Fragments arranged by size:
4545 1063 69
XmnI GAAnn'nnTTC
Cuts at: 2081 2343 3806 2081
Size: 262 1463 3952
Fragments arranged by size:
3952 1463 262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI XcmI