Restriction map for
pFN21AE6075
(Circular) (Six-base) MAPSORT of: pFN21AE6075.seq Check: 9071 from: 1 to: 5674
pFN21AE6075 5674 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2526 3017 1937
Size: 589 491 4594
Fragments arranged by size:
4594 589 491
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 3737 5121 5389 1632
Size: 629 1476 1384 268 1917
Fragments arranged by size:
1917 1476 1384 629 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 4573 5550 1203
Size: 3370 977 1327
Fragments arranged by size:
3370 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2822 1184
Size: 1638 4036
AlwNI CAG_nnn'CTG
Cuts at: 3417 4989 3417
Size: 1572 4102
ApaLI G'TGCA_C
Cuts at: 2797 3731 4887 2797
Size: 934 1156 3584
Fragments arranged by size:
3584 1156 934
ApoI r'AATT_y
Cuts at: 1493 2857 2983 3209 3361 5470 1493
Size: 1364 126 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152 126
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2967 2999 1870
Size: 73 1024 32 4545
Fragments arranged by size:
4545 1024 73 32
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2759 2995 4450 618
Size: 325 217 627 972 236 1455 1842
Fragments arranged by size:
1842 1455 972 627 325 236 217
BanII G_rGCy'C
Cuts at: 1391 2055 2993 1391
Size: 664 938 4072
Fragments arranged by size:
4072 938 664
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 2650 928
Size: 971 199 552 3952
Fragments arranged by size:
3952 971 552 199
BbvCI CC'TCA_GC
Cuts at: 2915 2915
Size: 5674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2476 2510 3865 3899 1903
Size: 34 539 34 1355 34 3678
Fragments arranged by size:
3678 1355 539 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BfrBI ATG'CAT
Cuts at: 2221 2221
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 2192 3091 2192
Size: 899 4775
Bme1580I G_kGCm'C
Cuts at: 1790 2801 3735 4891 1790
Size: 1011 934 1156 2573
Fragments arranged by size:
2573 1156 1011 934
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2788 2929 3405
Size: 416 53 83 399 24 1371 141 476
Cuts at: 3405 4363 442
Size: 958 1753
Fragments arranged by size:
1753 1371 958 476 416 399 141 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4334 1239
Size: 3095 2579
Bpu10I CC'TnA_GC
Cuts at: 2895 2915 2895
Size: 20 5654
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 3799 4664 4962 2066
Size: 1733 865 298 2778
Fragments arranged by size:
2778 1733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2585 2776 4337 882
Size: 617 1086 191 1561 2219
Fragments arranged by size:
2219 1561 1086 617 191
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2877 4107 4505 4779 4926 5337 2877
Size: 1230 398 274 147 411 3214
Fragments arranged by size:
3214 1230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2609 2612 2615 2932 1186
Size: 949 474 3 3 317 3928
Fragments arranged by size:
3928 949 474 317 3 3
BseYI C'CCAG_C
Cuts at: 2163 2301 2548 4877 5373 2163
Size: 138 247 2329 496 2464
Fragments arranged by size:
2464 2329 496 247 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 2801 3735 3820 4891 2409
Size: 392 934 85 1071 3192
Fragments arranged by size:
3192 1071 934 392 85
BsmI GAATG_Cn'
Cuts at: 2613 2940 3185 3278 2613
Size: 327 245 93 5009
Fragments arranged by size:
5009 327 245 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2409 2801 2993 3735 3820 4891
Size: 399 265 354 392 192 742 85 1071
Cuts at: 4891 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 392 354 265 192
85
BspEI T'CCGG_A
Cuts at: 2877 5337 2877
Size: 2460 3214
BspHI T'CATG_A
Cuts at: 2409 3563 5293 2409
Size: 1154 1730 2790
Fragments arranged by size:
2790 1730 1154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 844
Size: 2185 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4318 4505 1700
Size: 110 2508 187 2869
Fragments arranged by size:
2869 2508 187 110
BsrGI T'GTAC_A
Cuts at: 96 2601 96
Size: 2505 3169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 2212 3004 3371 3756 3773 5225 5311 5323
Size: 240 792 367 385 17 1452 86 12
Cuts at: 5323 5669 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 792 385 367 346 240 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2097 513
Size: 552 1032 4090
Fragments arranged by size:
4090 1032 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 3190 3984 4004 2390
Size: 800 794 20 4060
Fragments arranged by size:
4060 800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DraIII CAC_nnn'GTG
Cuts at: 2937 2937
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639
Size: 54 1425 50 275 18 1291 889 1629
Cuts at: 5639 8
Size: 43
Fragments arranged by size:
1629 1425 1291 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2902 3604 1353
Size: 707 842 702 3423
Fragments arranged by size:
3423 842 707 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 3737 4334 5121 5389 1239
Size: 393 629 1476 597 787 268 1524
Fragments arranged by size:
1524 1476 787 629 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2790 2790
Size: 5674
EcoRI G'AATT_C
Cuts at: 2857 2983 3361 2857
Size: 126 378 5170
Fragments arranged by size:
5170 378 126
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2857 2889 2913 2945 4315
Size: 32 135 32 1285 32 24 32 1370
Cuts at: 4315 4347 4389 4421 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1370 1285 135 42 32 32 32
32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 3018 3264 669
Size: 988 281 1080 246 3079
Fragments arranged by size:
3079 1080 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 2954 3273 2954
Size: 319 5355
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4788 4972 1305
Size: 3483 184 2007
Fragments arranged by size:
3483 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 2681 3632 3991 4150 518
Size: 648 1416 99 951 359 159 2042
Fragments arranged by size:
2042 1416 951 648 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 2552 3090 3767 4915 5160 1606
Size: 946 538 677 1148 245 2120
Fragments arranged by size:
2120 1148 946 677 538 245
NcoI C'CATG_G
Cuts at: 513 1065 2097 513
Size: 552 1032 4090
Fragments arranged by size:
4090 1032 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NsiI A_TGCA'T
Cuts at: 2223 2223
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2225 3032 4577 1132
Size: 97 996 807 1545 2229
Fragments arranged by size:
2229 1545 996 807 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2790 2790
Size: 5674
PshAI GACnn'nnGTC
Cuts at: 1613 2788 1613
Size: 1175 4499
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PstI C_TGCA'G
Cuts at: 830 3026 830
Size: 2196 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
PvuII CAG'CTG
Cuts at: 2552 2552
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2060
Size: 5674
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2833 3022 4157 4838 5029 826
Size: 219 1788 189 1135 681 191 1471
Fragments arranged by size:
1788 1471 1135 681 219 191 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 3778 4679 4941 820
Size: 197 926 102 1733 901 262 1553
Fragments arranged by size:
1733 1553 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2097 3113 513
Size: 552 1032 1016 3074
Fragments arranged by size:
3074 1032 1016 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2948 3701 3718 3871 4056 4501 325
Size: 1255 1368 753 17 153 185 445 1498
Fragments arranged by size:
1498 1368 1255 753 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 3920 5632
Size: 274 80 33 51 1443 624 1319 1712
Cuts at: 5632 96
Size: 138
Fragments arranged by size:
1712 1443 1319 624 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 3803 2340
Size: 1463 4211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI