Restriction map for
pFN21AE6070
(Circular) (Six-base) MAPSORT of: pFN21AE6070.seq Check: 3284 from: 1 to: 5668
pFN21AE6070 5668 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2431 2431
Size: 5668
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2857 2989 2857
Size: 132 5536
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2519 3731 5115 5383 1632
Size: 887 1212 1384 268 1917
Fragments arranged by size:
1917 1384 1212 887 268
AfeI AGC'GCT
Cuts at: 2504 2504
Size: 5668
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 2950 1166
Size: 1784 3884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2414 1184
Size: 1230 4438
AlwNI CAG_nnn'CTG
Cuts at: 3411 4983 3411
Size: 1572 4096
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2904 2977 3203 3355 5464 1493
Size: 1411 73 226 152 2109 1697
Fragments arranged by size:
2109 1697 1411 226 152 73
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2993 1870
Size: 73 1050 4545
Fragments arranged by size:
4545 1050 73
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2857 2989 4444 618
Size: 325 217 627 1070 132 1455 1842
Fragments arranged by size:
1842 1455 1070 627 325 217 132
BanII G_rGCy'C
Cuts at: 1391 2610 2722 2987 1391
Size: 1219 112 265 4072
Fragments arranged by size:
4072 1219 265 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2325 2540 928
Size: 971 426 215 4056
Fragments arranged by size:
4056 971 426 215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4279 136
Size: 107 122 71 631 605 2607 1525
Fragments arranged by size:
2607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 2447 2687 3085 2447
Size: 240 398 5030
Fragments arranged by size:
5030 398 240
Bme1580I G_kGCm'C
Cuts at: 1790 3729 4885 1790
Size: 1939 1156 2573
Fragments arranged by size:
2573 1939 1156
BmgBI CAC'GTC
Cuts at: 1318 2152 1318
Size: 834 4834
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3399 4357 442
Size: 416 53 83 399 24 1982 958 1753
Fragments arranged by size:
1982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2210 1056
Size: 1154 4514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2576 4328 1239
Size: 1337 1752 2579
Fragments arranged by size:
2579 1752 1337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 4331 882
Size: 617 582 2250 2219
Fragments arranged by size:
2250 2219 617 582
BsaAI yAC'GTr
Cuts at: 493 1204 2380 3459 493
Size: 711 1176 1079 2702
Fragments arranged by size:
2702 1176 1079 711
BsaBI GATnn'nnATC
Cuts at: 2288 5662 2288
Size: 3374 2294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2504 2534 1178
Size: 30 165 30 1101 30 4312
Fragments arranged by size:
4312 1101 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2462 1186
Size: 1276 4392
BseYI C'CCAG_C
Cuts at: 2254 4871 5367 2254
Size: 2617 496 2555
Fragments arranged by size:
2617 2555 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2608 1163
Size: 573 872 4223
Fragments arranged by size:
4223 872 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2470 2688 3729 3814 4885 2470
Size: 218 1041 85 1071 3253
Fragments arranged by size:
3253 1071 1041 218 85
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2470 2610 2688 2722 2987 3729 3814
Size: 399 680 140 78 34 265 742 85
Cuts at: 3814 4885 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 680 399 265 140 85
78 34
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 2562 3557 5287 2562
Size: 995 1730 2943
Fragments arranged by size:
2943 1730 995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2431 3023 844
Size: 1587 592 3489
Fragments arranged by size:
3489 1587 592
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2691 4163 4337 65
Size: 1183 1443 1472 174 1396
Fragments arranged by size:
1472 1443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 2824 96
Size: 2728 2940
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstXI CCAn_nnnn'nTGG
Cuts at: 2254 2254
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2088 2998 3365 3750 3767 5219 5305 5317
Size: 116 910 367 385 17 1452 86 12
Cuts at: 5317 5663 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 910 385 367 346 116 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2080 2176 2628 513
Size: 552 1015 96 452 3553
Fragments arranged by size:
3553 1015 552 452 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2780 487
Size: 683 56 1554 3375
Fragments arranged by size:
3375 1554 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 2396 3184 3978 3998 2240
Size: 156 788 794 20 3910
Fragments arranged by size:
3910 794 788 156 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2138 2173 529
Size: 35 1574 35 4024
Fragments arranged by size:
4024 1574 35 35
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2411 4675 809
Size: 507 1095 2264 1802
Fragments arranged by size:
2264 1802 1095 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 3115 4004
Size: 54 1425 50 275 18 552 733 889
Cuts at: 4004 5633 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 733 552 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 2765 2837 3598 1353
Size: 965 447 72 761 3423
Fragments arranged by size:
3423 965 761 447 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2576 3731 4328 5115 5383 1239
Size: 393 887 57 1155 597 787 268 1524
Fragments arranged by size:
1524 1155 887 787 597 393 268 57
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2058 2058
Size: 5668
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2506 4815 1678
Size: 828 2309 2531
Fragments arranged by size:
2531 2309 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373
Size: 32 135 32 2737 32 42 32 2626
Fragments arranged by size:
2737 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2861 2993 2861
Size: 132 5536
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3846
Fragments arranged by size:
3846 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2425 2523 2601 2745 2771 2950 3626
Size: 648 1259 98 78 144 26 179 676
Cuts at: 3626 3985 4144 518
Size: 359 159 2042
Fragments arranged by size:
2042 1259 676 648 359 179 159 144
98 78 26
MspA1I CmG'CkG
Cuts at: 1606 3084 3761 4909 5154 1606
Size: 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2080 2176 513
Size: 552 1015 96 4005
Fragments arranged by size:
4005 1015 552 96
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 2206 1052
Size: 1154 4514
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2206 2430 3026 4571 1132
Size: 97 977 224 596 1545 2229
Fragments arranged by size:
2229 1545 977 596 224 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2530 1108
Size: 1422 4246
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 2939 3238 2939
Size: 299 5369
PstI C_TGCA'G
Cuts at: 830 3020 830
Size: 2190 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SapI GCTCTTCn'nnn_
Cuts at: 2318 2765 2318
Size: 447 5221
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 2884 3016 4151 4832 5023 826
Size: 219 1839 132 1135 681 191 1471
Fragments arranged by size:
1839 1471 1135 681 219 191 132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3772 4673 4935 820
Size: 197 926 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 2206 3026 1132
Size: 1074 820 3774
Fragments arranged by size:
3774 1074 820
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 2226 1517
Size: 709 4959
StyI C'CwwG_G
Cuts at: 513 1065 2062 2080 2176 3107 513
Size: 552 997 18 96 931 3074
Fragments arranged by size:
3074 997 931 552 96 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2621 3695 3712 3865 4050 4495 325
Size: 1255 1041 1074 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1074 1041 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2159 2187 2752
Size: 274 80 33 51 1443 182 28 565
Cuts at: 2752 2824 3914 5626 96
Size: 72 1090 1712 138
Fragments arranged by size:
1712 1443 1090 565 274 182 138 80
72 51 33 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 2068 3004 2068
Size: 936 4732
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 2504 3797 2504
Size: 1293 4375
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI